Restriction map for
pFN21AB7788
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccIII AciI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BclI BcnI
BfrI BglI BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmAI BsmBI BsoBI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstEII BstPI BstXI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraIII
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HapII HhaI Hin1I
HinP1I HpaI HpaII KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MspI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NsiI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 1001 GTCTAC GT^[AC][GT]AC
AccII 351 CGCG CG^CG
AclI 1484 AACGTT AA^CGTT
AcsI 117 GAATTT [GA]^AATT[CT]
AocI 74 CCTCAGG CC^T[ACGT]AGG
ApoI 117 GAATTT [GA]^AATT[CT]
AseI 1027 ATTAAT AT^TAAT
AsnI 1027 ATTAAT AT^TAAT
AspHI 381 GTGCTC G[AT]GC[AT]^C
BbsI 15 GAAGAC GAAGAC(2/6)
BbvI 102 GCTGC GCAGC(8/12)
BciVI 1365 GTATCC GTATCC(6/5)
BmyI 381 GTGCTC G[AGT]GC[ACT]^C
BpuAI 15 GAAGAC GAAGAC(2/6)
BsiHKAI 381 GTGCTC G[AT]GC[AT]^C
BsiYI 504 CCATTGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 504 CCATTGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 1270 GGGAC GGGAC(10/14)
Bsp1286I 381 GTGCTC G[AGT]GC[ACT]^C
BstAPI 563 GCATTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 474 TTCGAA TT^CGAA
BstF5I 604 GGATG GGATG(2/0)
BstUI 351 CGCG CG^CG
Bsu36I 74 CCTCAGG CC^T[ACGT]AGG
DraII 1152 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 1154 GACCTGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 74 CCTCAGG CC^T[ACGT]AGG
EcoO109I 1152 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 1401 CCTTGG C^C[AT][AT]GG
Fnu4HI 102 GCTGC GC^[ACGT]GC
FokI 604 GGATG GGATG(9/13)
HaeIII 620 GGCC GG^CC
HgaI 1128 GCGTC GACGC(5/10)
HincII 837 GTTGAC GT[CT]^[GA]AC
HindII 837 GTTGAC GT[CT]^[GA]AC
HindIII 942 AAGCTT A^AGCTT
ItaI 102 GCTGC GC^[ACGT]GC
MaeII 1485 ACGT A^CGT
MaeIII 1406 GTGAC ^GT[ACGT]AC
MspA1I 226 CAGCTG C[AC]G^C[GT]G
MvnI 351 CGCG CG^CG
NlaIV 272 GGAACC GG[ACGT]^[ACGT]CC
NruI 350 TCGCGA TCG^CGA
NspV 474 TTCGAA TT^CGAA
PpuMI 1152 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 1027 ATTAAT AT^TAAT
Psp1406I 1484 AACGTT AA^CGTT
PvuII 226 CAGCTG CAG^CTG
SexAI 1155 ACCTGGT A^CC[AT]GGT
SfuI 474 TTCGAA TT^CGAA
StyI 1401 CCTTGG C^C[AT][AT]GG
TaqI 475 TCGA T^CGA
TseI 102 GCTGC G^C[AT]GC
Tsp45I 1406 GTGAC ^GT[GC]AC
TthHB8I 475 TCGA T^CGA
XcmI 259 CCAAGATCATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwNI 554 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
1268 CAGGGACTG
AvaII 894 GGACC G^G[AT]CC
1153 GGACC
BfaI 425 CTAG C^TAG
713 CTAG
BglII 741 AGATCT A^GATCT
1177 AGATCT
BsaJI 1369 CCAGGG C^C[ACGT][ACGT]GG
1401 CCTTGG
Bsp1407I 408 TGTACA T^GTACA
1445 TGTACA
BsrGI 408 TGTACA T^GTACA
1445 TGTACA
BstYI 741 AGATCT [GA]^GATC[CT]
1177 AGATCT
Cfr13I 894 GGACC G^G[ACGT]CC
1153 GGACC
DraI 1060 TTTAAA TTT^AAA
1488 TTTAAA
MaeI 425 CTAG C^TAG
713 CTAG
MflI 741 AGATCT [GA]^GATC[CT]
1177 AGATCT
MwoI 343 GCAAATATCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
563 GCATTTTCTGC
NspI 218 GCATGT [GA]CATG^[CT]
572 GCATGT
PstI 103 CTGCAG CTGCA^G
1094 CTGCAG
Sau96I 894 GGACC G^G[ACGT]CC
1153 GGACC
SfcI 103 CTGCAG C^T[GA][CT]AG
1094 CTGCAG
SspBI 408 TGTACA T^GTACA
1445 TGTACA
XhoII 741 AGATCT [GA]^GATC[CT]
1177 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AfaI AluI AlwI Asp700I BsmI
BsrI BssKI BstNI Cac8I DdeI
DpnII EarI EcoRII HinfI HphI
Ksp632I MboI MboII MnlI MseI
MslI MvaI NdeII NlaIII PleI
RsaI Sau3AI ScrFI SfaNI TfiI
Tru9I Tsp509I TspRI XmnI