Restriction map for
pFN21AB7788
(Circular) (Six-base) MAPSORT of: pFN21AB7788.seq Check: 9390 from: 1 to: 6247
pFN21AB7788 6247 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4057 278
Size: 53 83 186 936 2521 2468
Fragments arranged by size:
2521 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3007 3590 1937
Size: 1070 583 4594
Fragments arranged by size:
4594 1070 583
Acc65I G'GTAC_C
Cuts at: 3568 3568
Size: 6247
AclI AA'CG_TT
Cuts at: 1563 3490 4374 4747 1563
Size: 1927 884 373 3063
Fragments arranged by size:
3063 1927 884 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2174 2745 2858 3498 4310 5694 5962 1632
Size: 542 571 113 640 812 1384 268 1917
Fragments arranged by size:
1917 1384 812 640 571 542 268 113
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6050
AflIII A'CryG_T
Cuts at: 1203 3544 5146 6123 1203
Size: 2341 1602 977 1327
Fragments arranged by size:
2341 1602 1327 977
AgeI A'CCGG_T
Cuts at: 5078 5078
Size: 6247
AhdI GACnn_n'nnGTC
Cuts at: 4976 4976
Size: 6247
AleI CACnn'nnGTG
Cuts at: 1166 2665 1166
Size: 1499 4748
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6247
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2481 2513 2481
Size: 32 6215
AlwNI CAG_nnn'CTG
Cuts at: 2564 3278 3990 5562 2564
Size: 714 712 1572 3249
Fragments arranged by size:
3249 1572 714 712
ApaLI G'TGCA_C
Cuts at: 4304 5460 4304
Size: 1156 5091
ApoI r'AATT_y
Cuts at: 1493 2122 3556 3782 3934 6043 1493
Size: 629 1434 226 152 2109 1697
Fragments arranged by size:
2109 1697 1434 629 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3033 4801 160
Size: 872 2001 1768 1606
Fragments arranged by size:
2001 1768 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6247
AvaI C'yCGr_G
Cuts at: 1870 1943 3572 1870
Size: 73 1629 4545
Fragments arranged by size:
4545 1629 73
BamHI G'GATC_C
Cuts at: 3577 3944 3577
Size: 367 5880
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3568 5023 618
Size: 325 217 627 1781 1455 1842
Fragments arranged by size:
1842 1781 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3566 1391
Size: 2175 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2027 928
Size: 971 128 5148
Fragments arranged by size:
5148 971 128
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4438 4472 1903
Size: 34 2501 34 3678
Fragments arranged by size:
3678 2501 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3381 4139 5349 3381
Size: 758 1210 4279
Fragments arranged by size:
4279 1210 758
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6247
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4858 136
Size: 107 122 71 631 605 3186 1525
Fragments arranged by size:
3186 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2746 3182 6242 2746
Size: 436 3060 2751
Fragments arranged by size:
3060 2751 436
BlpI GC'TnA_GC
Cuts at: 3664 3664
Size: 6247
Bme1580I G_kGCm'C
Cuts at: 1790 4308 5464 1790
Size: 2518 1156 2573
Fragments arranged by size:
2573 2518 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6247
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3978 4936 442
Size: 416 53 83 399 24 2561 958 1753
Fragments arranged by size:
2561 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6247
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4907 1239
Size: 3668 2579
Bpu10I CC'TnA_GC
Cuts at: 3271 3271
Size: 6247
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4372 5237 5535 4372
Size: 865 298 5084
Fragments arranged by size:
5084 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4910 882
Size: 617 3411 2219
Fragments arranged by size:
3411 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4038 493
Size: 711 2834 2702
Fragments arranged by size:
2834 2702 711
BsaBI GATnn'nnATC
Cuts at: 6241 6241
Size: 6247
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4054 4436 5071 275
Size: 53 83 186 936 2521 382 635 1451
Fragments arranged by size:
2521 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4680 5078 5352 5499 5910 4680
Size: 398 274 147 411 5017
Fragments arranged by size:
5017 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2037 2067 1178
Size: 30 165 30 634 30 5358
Fragments arranged by size:
5358 634 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6247
BseYI C'CCAG_C
Cuts at: 5450 5946 5450
Size: 496 5751
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5674
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3697 4458 5486 1540
Size: 275 186 1696 761 1028 2301
Fragments arranged by size:
2301 1696 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2390 4308 4393 5464 2390
Size: 1918 85 1071 3173
Fragments arranged by size:
3173 1918 1071 85
BsmI GAATG_Cn'
Cuts at: 2760 2970 3325 3758 3851 2760
Size: 210 355 433 93 5156
Fragments arranged by size:
5156 433 355 210 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2390 3566 4308 4393 5464 1391
Size: 399 600 1176 742 85 1071 2174
Fragments arranged by size:
2174 1176 1071 742 600 399 85
BspEI T'CCGG_A
Cuts at: 5910 5910
Size: 6247
BspHI T'CATG_A
Cuts at: 4136 5866 4136
Size: 1730 4517
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3602 844
Size: 2758 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4134 1394
Size: 429 2311 3507
Fragments arranged by size:
3507 2311 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4742 4916 65
Size: 1183 3494 174 1396
Fragments arranged by size:
3494 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4891 5078 1700
Size: 110 3081 187 2869
Fragments arranged by size:
3081 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2413 3450 96
Size: 2317 1037 2893
Fragments arranged by size:
2893 2317 1037
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6247
BssSI C'ACGA_G
Cuts at: 4307 5319 4307
Size: 1012 5235
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2574 794
Size: 1780 4467
BstBI TT'CG_AA
Cuts at: 2480 5051 2480
Size: 2571 3676
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6247
BstYI r'GATC_y
Cuts at: 1972 2746 3182 3577 3944 4329 4346 5798 5884
Size: 774 436 395 367 385 17 1452 86
Cuts at: 5884 5896 6242 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 774 436 395 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2080 2080
Size: 6247
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4756
Fragments arranged by size:
4756 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3012 487
Size: 683 56 1786 3722
Fragments arranged by size:
3722 1786 683 56
BtsI GCAGTG_nn'
Cuts at: 2117 2390 3763 4557 4577 2117
Size: 273 1373 794 20 3787
Fragments arranged by size:
3787 1373 794 273 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6247
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6212
DraI TTT'AAA
Cuts at: 3067 3495 3551 3067
Size: 428 56 5763
Fragments arranged by size:
5763 428 56
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3165 5254 809
Size: 507 1849 2089 1802
Fragments arranged by size:
2089 1849 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3694 4583 6212
Size: 54 1425 50 275 18 1864 889 1629
Cuts at: 6212 8
Size: 43
Fragments arranged by size:
1864 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3694 1537
Size: 275 1882 4090
Fragments arranged by size:
4090 1882 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2477 2479 3235 3432 3506 4177 1353
Size: 1124 2 756 197 74 671 3423
Fragments arranged by size:
3423 1124 756 671 197 74 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4834 5208 5354 387
Size: 1064 138 3245 374 146 1280
Fragments arranged by size:
3245 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2174 2745 2858 3498 4310 4907 5694
Size: 393 542 571 113 640 812 597 787
Cuts at: 5694 5962 1239
Size: 268 1524
Fragments arranged by size:
1524 812 787 640 597 571 542 393
268 113
EcoO109I rG'GnC_Cy
Cuts at: 3158 3158
Size: 6247
EcoRI G'AATT_C
Cuts at: 3556 3934 3556
Size: 378 5869
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2289 2289
Size: 6247
FspI TGC'GCA
Cuts at: 4753 4753
Size: 6247
HaeII r_GCGC'y
Cuts at: 1678 5394 1678
Size: 3716 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2546 2578 2836 2868 4888
Size: 32 135 32 974 32 258 32 2020
Cuts at: 4888 4920 4962 4994 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2020 974 258 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2844 3591 3837 669
Size: 988 281 906 747 246 3079
Fragments arranged by size:
3079 988 906 747 281 246
HindIII A'AGCT_T
Cuts at: 748 2947 748
Size: 2199 4048
HpaI GTT'AAC
Cuts at: 3837 3837
Size: 6247
KpnI G_GTAC'C
Cuts at: 3572 3572
Size: 6247
MfeI C'AATT_G
Cuts at: 3846 3846
Size: 6247
MluI A'CGCG_T
Cuts at: 6123 6123
Size: 6247
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3488 5361 5545 1305
Size: 2183 1873 184 2007
Fragments arranged by size:
2183 2007 1873 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4425
Fragments arranged by size:
4425 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2448 2665 3024 3086 3398 4205 4564
Size: 648 1282 217 359 62 312 807 359
Cuts at: 4564 4723 518
Size: 159 2042
Fragments arranged by size:
2042 1282 807 648 359 359 312 217
159 62
MspA1I CmG'CkG
Cuts at: 1606 2233 3663 4340 5488 5733 1606
Size: 627 1430 677 1148 245 2120
Fragments arranged by size:
2120 1430 1148 677 627 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4756
Fragments arranged by size:
4756 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6247
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6247
NotI GC'GGCC_GC
Cuts at: 3694 3694
Size: 6247
NruI TCG'CGA
Cuts at: 2357 2357
Size: 6247
NspI r_CATG'y
Cuts at: 1132 1229 2227 2581 3548 3605 5150 1132
Size: 97 998 354 967 57 1545 2229
Fragments arranged by size:
2229 1545 998 967 354 97 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6247
PciI A'CATG_T
Cuts at: 3544 5146 3544
Size: 1602 4645
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 5345
PfoI T'CCnGG_A
Cuts at: 3013 3013
Size: 6247
PmeI GTTT'AAAC
Cuts at: 3551 3551
Size: 6247
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4316 4348 5829 5861 4316
Size: 32 1481 32 4702
Fragments arranged by size:
4702 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3158 3158
Size: 6247
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6247
PsiI TTA'TAA
Cuts at: 3817 3817
Size: 6247
PstI C_TGCA'G
Cuts at: 830 2112 3103 3599 830
Size: 1282 991 496 3478
Fragments arranged by size:
3478 1282 991 496
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6247
PvuII CAG'CTG
Cuts at: 2233 2233
Size: 6247
SalI G'TCGA_C
Cuts at: 1936 3589 1936
Size: 1653 4594
SbfI CC_TGCA'GG
Cuts at: 3599 3599
Size: 6247
ScaI AGT'ACT
Cuts at: 4495 6207 4495
Size: 1712 4535
SexAI A'CCwGG_T
Cuts at: 3160 3160
Size: 6247
SfcI C'TryA_G
Cuts at: 826 1045 2108 3099 3595 4730 5411 5602 826
Size: 219 1063 991 496 1135 681 191 1471
Fragments arranged by size:
1471 1135 1063 991 681 496 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6247
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6247
SmaI CCC'GGG
Cuts at: 1872 3574 1872
Size: 1702 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4351 5252 5514 820
Size: 197 926 2408 901 262 1553
Fragments arranged by size:
2408 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4038 493
Size: 3545 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6247
SphI G_CATG'C
Cuts at: 1132 3605 1132
Size: 2473 3774
SspI AAT'ATT
Cuts at: 5 52 4171 5
Size: 47 4119 2081
Fragments arranged by size:
4119 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6247
StyI C'CwwG_G
Cuts at: 513 1065 2004 3406 3686 513
Size: 552 939 1402 280 3074
Fragments arranged by size:
3074 1402 939 552 280
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4274 4291 4444 4629 5074 325
Size: 1255 2694 17 153 185 445 1498
Fragments arranged by size:
2694 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2044 2413 2833
Size: 274 80 33 51 1443 67 369 420
Cuts at: 2833 3450 4493 6205 96
Size: 617 1043 1712 138
Fragments arranged by size:
1712 1443 1043 617 420 369 274 138
80 67 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3268 3300 6207 6239 3268
Size: 32 2907 32 3276
Fragments arranged by size:
3276 2907 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6247
XbaI T'CTAG_A
Cuts at: 3583 3583
Size: 6247
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2271 2271
Size: 6247
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6247
XmaI C'CCGG_G
Cuts at: 1870 3572 1870
Size: 1702 4545
XmnI GAAnn'nnTTC
Cuts at: 2748 3323 3526 4376 2748
Size: 575 203 850 4619
Fragments arranged by size:
4619 850 575 203
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4055 276
Size: 53 83 186 936 2521 2468
Fragments arranged by size:
2521 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NruI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI