################################## # NO CUT ENZYMES ( 187 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AfaI AflII AflIII AgeI AhdI AlwNI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BanII BbeI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeI MamI MboII MfeI MluI MluNI MroI MscI MseI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AccI 103 GTCTAC GT^[AC][GT]AC AcsI 364 GAATTC [GA]^AATT[CT] Alw44I 4 GTGCAC G^TGCAC ApaLI 4 GTGCAC G^TGCAC ApoI 364 GAATTC [GA]^AATT[CT] AvaII 113 GGACC G^G[AT]CC BamHI 297 GGATCC G^GATCC BbrPI 292 CACGTG CAC^GTG BsaAI 292 CACGTG [CT]AC^GT[GA] BseRI 19 GAGGAG GAGGAG(10/8) BsiYI 83 CCCTGGGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 83 CCCTGGGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsrI 376 CCAGT ACTGG(1/-1) BstF5I 63 GGATG GGATG(2/0) BstXI 348 CCATCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 80 AGGCCCT [GA]G^G[ACGT]CC[CT] DraIII 263 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO109I 80 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoRI 364 GAATTC G^AATTC EcoT14I 109 CCTTGG C^C[AT][AT]GG FokI 63 GGATG GGATG(9/13) NlaIII 190 CATG CATG^ PmaCI 292 CACGTG CAC^GTG PmlI 292 CACGTG CAC^GTG SfaNI 157 GATGC GCATC(5/9) SmlI 244 CTCAAG C^T[CT][GA]AG StyI 109 CCTTGG C^C[AT][AT]GG Tsp45I 281 GTGAC ^GT[GC]AC Tsp509I 365 AATT ^AATT ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AluI 272 AGCT AG^CT 287 AGCT AocI 16 CCTGAGG CC^T[ACGT]AGG 175 CCTAAGG AspHI 4 GTGCAC G[AT]GC[AT]^C 202 GTGCTC BanI 208 GGTGCC G^G[CT][GA]CC 250 GGCACC BsiHKAI 4 GTGCAC G[AT]GC[AT]^C 202 GTGCTC BstYI 141 GGATCT [GA]^GATC[CT] 297 GGATCC Bsu36I 16 CCTGAGG CC^T[ACGT]AGG 175 CCTAAGG DpnII 142 GATC ^GATC 298 GATC Eco81I 16 CCTGAGG CC^T[ACGT]AGG 175 CCTAAGG HinfI 12 GACTC G^A[ACGT]TC 130 GAGTC MaeIII 34 GTTAC ^GT[ACGT]AC 281 GTGAC MboI 142 GATC ^GATC 298 GATC MflI 141 GGATCT [GA]^GATC[CT] 297 GGATCC MwoI 31 GCCGTTACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 406 GCTGGTGTGGC NdeII 142 GATC ^GATC 298 GATC PleI 12 GACTC GAGTC(4/5) 130 GAGTC Sau3AI 142 GATC ^GATC 298 GATC XhoII 141 GGATCT [GA]^GATC[CT] 297 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AlwI BbvI BmyI BsaJI BsgI Bsp1286I BssKI BstNI Cac8I Cfr13I DdeI EcoRII Fnu4HI HaeIII HphI ItaI MaeII MnlI MvaI NlaIV Sau96I ScrFI TseI TspRI