(Circular) (Six-base) MAPSORT of: pFN21AA1926.seq Check: 1951 from: 1 to: 5911 pFN21AA1926 5911 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2158 2158 Size: 5911 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2298 3721 278 Size: 53 83 186 936 762 1423 2468 Fragments arranged by size: 2468 1423 936 762 186 83 53 AccI GT'mk_AC Cuts at: 1937 3087 3254 1937 Size: 1150 167 4594 Fragments arranged by size: 4594 1150 167 Acc65I G'GTAC_C Cuts at: 3232 3232 Size: 5911 AclI AA'CG_TT Cuts at: 1563 4038 4411 1563 Size: 2475 373 3063 Fragments arranged by size: 3063 2475 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2858 3974 5358 5626 1632 Size: 1226 1116 1384 268 1917 Fragments arranged by size: 1917 1384 1226 1116 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5714 AflIII A'CryG_T Cuts at: 1203 4810 5787 1203 Size: 3607 977 1327 Fragments arranged by size: 3607 1327 977 AgeI A'CCGG_T Cuts at: 4742 4742 Size: 5911 AhdI GACnn_n'nnGTC Cuts at: 4640 4640 Size: 5911 AleI CACnn'nnGTG Cuts at: 1166 2184 2511 1166 Size: 1018 327 4566 Fragments arranged by size: 4566 1018 327 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5911 AlwNI CAG_nnn'CTG Cuts at: 2081 2162 3654 5226 2081 Size: 81 1492 1572 2766 Fragments arranged by size: 2766 1572 1492 81 ApaLI G'TGCA_C Cuts at: 2114 3968 5124 2114 Size: 1854 1156 2901 Fragments arranged by size: 2901 1854 1156 ApoI r'AATT_y Cuts at: 1493 3220 3446 3598 5707 1493 Size: 1727 226 152 2109 1697 Fragments arranged by size: 2109 1727 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4465 160 Size: 872 3433 1606 Fragments arranged by size: 3433 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5911 AvaI C'yCGr_G Cuts at: 1870 1943 2016 2682 3236 1870 Size: 73 73 666 554 4545 Fragments arranged by size: 4545 666 554 73 73 BamHI G'GATC_C Cuts at: 3241 3608 3241 Size: 367 5544 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2189 3121 3232 4687 618 Size: 325 217 627 402 932 111 1455 1842 Fragments arranged by size: 1842 1455 932 627 402 325 217 111 BanII G_rGCy'C Cuts at: 1391 2141 2691 3230 1391 Size: 750 550 539 4072 Fragments arranged by size: 4072 750 550 539 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2290 928 Size: 971 391 4549 Fragments arranged by size: 4549 971 391 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2966 3000 3034 3170 3204 4102 4136 Size: 34 1029 34 34 136 34 898 34 Cuts at: 4136 1903 Size: 3678 Fragments arranged by size: 3678 1029 898 136 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3803 5013 3803 Size: 1210 4701 BclI T'GATC_A Cuts at: 1548 1548 Size: 5911 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4522 136 Size: 107 122 71 631 605 2850 1525 Fragments arranged by size: 2850 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5906 5906 Size: 5911 BlpI GC'TnA_GC Cuts at: 2352 3328 2352 Size: 976 4935 Bme1580I G_kGCm'C Cuts at: 1790 2118 3972 5128 1790 Size: 328 1854 1156 2573 Fragments arranged by size: 2573 1854 1156 328 BmgBI CAC'GTC Cuts at: 1318 2808 3208 1318 Size: 1490 400 4021 Fragments arranged by size: 4021 1490 400 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3642 4600 442 Size: 416 53 83 399 24 2225 958 1753 Fragments arranged by size: 2225 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5911 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2155 2155 Size: 5911 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2144 3087 4571 1239 Size: 905 943 1484 2579 Fragments arranged by size: 2579 1484 943 905 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2240 4036 4901 5199 2240 Size: 1796 865 298 2952 Fragments arranged by size: 2952 1796 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4574 882 Size: 617 3075 2219 Fragments arranged by size: 3075 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2119 3702 493 Size: 711 915 1583 2702 Fragments arranged by size: 2702 1583 915 711 BsaBI GATnn'nnATC Cuts at: 5905 5905 Size: 5911 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2061 2247 2295 2793 Size: 53 83 186 936 528 186 48 498 Cuts at: 2793 3063 3084 3718 4100 4735 275 Size: 270 21 634 382 635 1451 Fragments arranged by size: 1451 936 635 634 528 498 382 270 186 186 83 53 48 21 BsaWI w'CCGG_w Cuts at: 2864 3112 4344 4742 5016 5163 5574 2864 Size: 248 1232 398 274 147 411 3201 Fragments arranged by size: 3201 1232 411 398 274 248 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2117 2147 1178 Size: 30 165 30 714 30 4942 Fragments arranged by size: 4942 714 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5911 BseYI C'CCAG_C Cuts at: 2316 3132 3138 3144 3162 5114 5610 2316 Size: 816 6 6 18 1952 496 2617 Fragments arranged by size: 2617 1952 816 496 18 6 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2582 2739 2941 3188 1163 Size: 573 846 157 202 247 3886 Fragments arranged by size: 3886 846 573 247 202 157 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2901 3361 4122 5150 1540 Size: 275 186 900 460 761 1028 2301 Fragments arranged by size: 2301 1028 900 761 460 275 186 BsiHKAI G_wGCw'C Cuts at: 2118 3112 3972 4057 5128 2118 Size: 994 860 85 1071 2901 Fragments arranged by size: 2901 1071 994 860 85 BsmI GAATG_Cn' Cuts at: 2327 3422 3515 2327 Size: 1095 93 4723 Fragments arranged by size: 4723 1095 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 2141 2691 3112 3230 3972 4057 Size: 399 328 23 550 421 118 742 85 Cuts at: 4057 5128 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 550 421 399 328 118 85 23 BspEI T'CCGG_A Cuts at: 2864 5574 2864 Size: 2710 3201 BspHI T'CATG_A Cuts at: 3800 5530 3800 Size: 1730 4181 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2158 2823 3266 844 Size: 1314 665 443 3489 Fragments arranged by size: 3489 1314 665 443 BsrBI CCG'CTC Cuts at: 1394 1823 3798 1394 Size: 429 1975 3507 Fragments arranged by size: 3507 1975 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4406 4580 65 Size: 1183 3158 174 1396 Fragments arranged by size: 3158 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2676 4555 4742 1700 Size: 110 866 1879 187 2869 Fragments arranged by size: 2869 1879 866 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5911 BssHII G'CGCG_C Cuts at: 1925 2037 2551 2873 2875 2987 3014 3174 3176 Size: 112 514 322 2 112 27 160 2 Cuts at: 3176 1925 Size: 4660 Fragments arranged by size: 4660 514 322 160 112 112 27 2 2 BssSI C'ACGA_G Cuts at: 3971 4983 3971 Size: 1012 4899 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5911 BstBI TT'CG_AA Cuts at: 4715 4715 Size: 5911 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5911 BstXI CCAn_nnnn'nTGG Cuts at: 2186 2449 2513 2186 Size: 263 64 5584 Fragments arranged by size: 5584 263 64 BstYI r'GATC_y Cuts at: 1972 3241 3608 3993 4010 5462 5548 5560 5906 Size: 1269 367 385 17 1452 86 12 346 Cuts at: 5906 1972 Size: 1977 Fragments arranged by size: 1977 1452 1269 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2049 2182 2233 2341 2512 2901 Size: 552 939 45 133 51 108 171 389 Cuts at: 2901 2961 513 Size: 60 3463 Fragments arranged by size: 3463 939 552 389 171 133 108 60 51 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5172 Fragments arranged by size: 5172 683 56 BtsI GCAGTG_nn' Cuts at: 2989 3427 4221 4241 2989 Size: 438 794 20 4659 Fragments arranged by size: 4659 794 438 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5911 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2253 2288 529 Size: 35 1689 35 4152 Fragments arranged by size: 4152 1689 35 35 DraI TTT'AAA Cuts at: 3215 3215 Size: 5911 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4918 809 Size: 507 3602 1802 Fragments arranged by size: 3602 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2098 2898 2904 Size: 54 1425 50 275 18 268 800 6 Cuts at: 2904 2973 3358 4247 5876 8 Size: 69 385 889 1629 43 Fragments arranged by size: 1629 1425 889 800 385 275 268 69 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2898 3358 1537 Size: 275 1086 460 4090 Fragments arranged by size: 4090 1086 460 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3083 3841 1353 Size: 1730 758 3423 Fragments arranged by size: 3423 1730 758 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4498 4872 5018 387 Size: 1064 138 2909 374 146 1280 Fragments arranged by size: 2909 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2144 2858 3087 3974 4571 5358 5626 Size: 393 512 714 229 887 597 787 268 Cuts at: 5626 1239 Size: 1524 Fragments arranged by size: 1524 887 787 714 597 512 393 268 229 EcoO109I rG'GnC_Cy Cuts at: 2312 2746 2312 Size: 434 5477 EcoRI G'AATT_C Cuts at: 3220 3598 3220 Size: 378 5533 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2551 2551 Size: 5911 FspI TGC'GCA Cuts at: 2753 3044 4417 2753 Size: 291 1373 4247 Fragments arranged by size: 4247 1373 291 HaeII r_GCGC'y Cuts at: 1678 2162 2425 5058 1678 Size: 484 263 2633 2531 Fragments arranged by size: 2633 2531 484 263 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2117 2149 4552 4584 4626 Size: 32 135 32 545 32 2403 32 42 Cuts at: 4626 4658 1373 Size: 32 2626 Fragments arranged by size: 2626 2403 545 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3255 3501 669 Size: 988 281 1317 246 3079 Fragments arranged by size: 3079 1317 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5911 HpaI GTT'AAC Cuts at: 3501 3501 Size: 5911 KpnI G_GTAC'C Cuts at: 3236 3236 Size: 5911 MfeI C'AATT_G Cuts at: 3510 3510 Size: 5911 MluI A'CGCG_T Cuts at: 5787 5787 Size: 5911 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2835 5025 5209 1305 Size: 1530 2190 184 2007 Fragments arranged by size: 2190 2007 1530 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2906 10 Size: 54 1425 343 1074 3015 Fragments arranged by size: 3015 1425 1074 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2184 2511 3869 4228 4387 518 Size: 648 1018 327 1358 359 159 2042 Fragments arranged by size: 2042 1358 1018 648 359 327 159 MspA1I CmG'CkG Cuts at: 1606 2051 2281 2596 2845 2920 2944 2955 2963 Size: 445 230 315 249 75 24 11 8 Cuts at: 2963 3327 4004 5152 5397 1606 Size: 364 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 445 364 315 249 245 230 75 24 11 8 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4420 Fragments arranged by size: 4420 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5911 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5911 NotI GC'GGCC_GC Cuts at: 3358 3358 Size: 5911 NspI r_CATG'y Cuts at: 1132 1229 3269 4814 1132 Size: 97 2040 1545 2229 Fragments arranged by size: 2229 2040 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5911 PciI A'CATG_T Cuts at: 4810 4810 Size: 5911 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5911 PfoI T'CCnGG_A Cuts at: 3057 3103 3057 Size: 46 5865 PmeI GTTT'AAAC Cuts at: 3215 3215 Size: 5911 PmlI CAC'GTG Cuts at: 2119 2119 Size: 5911 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3980 4012 5493 5525 3980 Size: 32 1481 32 4366 Fragments arranged by size: 4366 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5911 PsiI TTA'TAA Cuts at: 3481 3481 Size: 5911 PstI C_TGCA'G Cuts at: 830 2361 2590 2943 3263 830 Size: 1531 229 353 320 3478 Fragments arranged by size: 3478 1531 353 320 229 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5911 PvuII CAG'CTG Cuts at: 2596 2920 2944 2596 Size: 324 24 5563 Fragments arranged by size: 5563 324 24 SacII CC_GC'GG Cuts at: 2052 2964 2052 Size: 912 4999 SalI G'TCGA_C Cuts at: 1936 3253 1936 Size: 1317 4594 SapI GCTCTTCn'nnn_ Cuts at: 3083 3083 Size: 5911 SbfI CC_TGCA'GG Cuts at: 3263 3263 Size: 5911 ScaI AGT'ACT Cuts at: 4159 5871 4159 Size: 1712 4199 SfcI C'TryA_G Cuts at: 826 1045 2357 2586 2939 3259 4394 5075 5266 Size: 219 1312 229 353 320 1135 681 191 Cuts at: 5266 826 Size: 1471 Fragments arranged by size: 1471 1312 1135 681 353 320 229 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5911 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5911 SmaI CCC'GGG Cuts at: 1872 2684 3238 1872 Size: 812 554 4545 Fragments arranged by size: 4545 812 554 SmlI C'TyrA_G Cuts at: 820 1017 1943 2255 4015 4916 5178 820 Size: 197 926 312 1760 901 262 1553 Fragments arranged by size: 1760 1553 926 901 312 262 197 SnaBI TAC'GTA Cuts at: 493 3702 493 Size: 3209 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5911 SphI G_CATG'C Cuts at: 1132 3269 1132 Size: 2137 3774 SspI AAT'ATT Cuts at: 5 52 3835 5 Size: 47 3783 2081 Fragments arranged by size: 3783 2081 47 StuI AGG'CCT Cuts at: 1517 3034 1517 Size: 1517 4394 StyI C'CwwG_G Cuts at: 513 1065 2004 2907 3350 513 Size: 552 939 903 443 3074 Fragments arranged by size: 3074 939 903 552 443 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2018 3938 3955 4108 4293 4738 325 Size: 1255 438 1920 17 153 185 445 1498 Fragments arranged by size: 1920 1498 1255 445 438 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4157 5869 96 Size: 274 80 33 51 1443 2180 1712 138 Fragments arranged by size: 2180 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2701 2733 5871 5903 2701 Size: 32 3138 32 2709 Fragments arranged by size: 3138 2709 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2246 1316 Size: 930 4981 XbaI T'CTAG_A Cuts at: 3247 3247 Size: 5911 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5911 XmaI C'CCGG_G Cuts at: 1870 2682 3236 1870 Size: 812 554 4545 Fragments arranged by size: 4545 812 554 XmnI GAAnn'nnTTC Cuts at: 4040 4040 Size: 5911 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2296 3719 276 Size: 53 83 186 936 762 1423 2468 Fragments arranged by size: 2468 1423 936 762 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI XcmI