(Circular) (Six-base) MAPSORT of: pFN21AE0527.seq Check: 1490 from: 1 to: 5437 pFN21AE0527 5437 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2476 2476 Size: 5437 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3247 278 Size: 53 83 186 936 1711 2468 Fragments arranged by size: 2468 1711 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2055 2338 2780 1937 Size: 118 283 442 4594 Fragments arranged by size: 4594 442 283 118 Acc65I G'GTAC_C Cuts at: 2758 2758 Size: 5437 AclI AA'CG_TT Cuts at: 1563 3564 3937 1563 Size: 2001 373 3063 Fragments arranged by size: 3063 2001 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3500 4884 5152 1632 Size: 1868 1384 268 1917 Fragments arranged by size: 1917 1868 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5240 AflIII A'CryG_T Cuts at: 1203 4336 5313 1203 Size: 3133 977 1327 Fragments arranged by size: 3133 1327 977 AgeI A'CCGG_T Cuts at: 4268 4268 Size: 5437 AhdI GACnn_n'nnGTC Cuts at: 2032 4166 2032 Size: 2134 3303 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5437 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2295 1184 Size: 1111 4326 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2435 2467 2435 Size: 32 5405 AlwNI CAG_nnn'CTG Cuts at: 2431 3180 4752 2431 Size: 749 1572 3116 Fragments arranged by size: 3116 1572 749 ApaLI G'TGCA_C Cuts at: 3494 4650 3494 Size: 1156 4281 ApoI r'AATT_y Cuts at: 1493 2746 2972 3124 5233 1493 Size: 1253 226 152 2109 1697 Fragments arranged by size: 2109 1697 1253 226 152 AseI AT'TA_AT Cuts at: 160 1032 3991 160 Size: 872 2959 1606 Fragments arranged by size: 2959 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5437 AvaI C'yCGr_G Cuts at: 1870 1943 2387 2762 1870 Size: 73 444 375 4545 Fragments arranged by size: 4545 444 375 73 BamHI G'GATC_C Cuts at: 2730 2767 3134 2730 Size: 37 367 5033 Fragments arranged by size: 5033 367 37 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2327 2383 2758 4213 618 Size: 325 217 627 540 56 375 1455 1842 Fragments arranged by size: 1842 1455 627 540 375 325 217 56 BanII G_rGCy'C Cuts at: 1391 2756 1391 Size: 1365 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4466 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3628 3662 1903 Size: 34 1691 34 3678 Fragments arranged by size: 3678 1691 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2268 3329 4539 2268 Size: 1061 1210 3166 Fragments arranged by size: 3166 1210 1061 BclI T'GATC_A Cuts at: 1548 1548 Size: 5437 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2705 4048 136 Size: 107 122 71 631 605 1033 1343 1525 Fragments arranged by size: 1525 1343 1033 631 605 122 107 71 BglII A'GATC_T Cuts at: 5432 5432 Size: 5437 BlpI GC'TnA_GC Cuts at: 2613 2854 2613 Size: 241 5196 Bme1580I G_kGCm'C Cuts at: 1790 2284 2388 3498 4654 1790 Size: 494 104 1110 1156 2573 Fragments arranged by size: 2573 1156 1110 494 104 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5437 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3168 4126 442 Size: 416 53 83 399 24 1751 958 1753 Fragments arranged by size: 1753 1751 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5437 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2462 2534 4097 1239 Size: 1223 72 1563 2579 Fragments arranged by size: 2579 1563 1223 72 Bpu10I CC'TnA_GC Cuts at: 2225 2225 Size: 5437 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2159 2665 3562 4427 4725 2159 Size: 506 897 865 298 2871 Fragments arranged by size: 2871 897 865 506 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4100 882 Size: 617 2601 2219 Fragments arranged by size: 2601 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3228 493 Size: 711 2024 2702 Fragments arranged by size: 2702 2024 711 BsaBI GATnn'nnATC Cuts at: 5431 5431 Size: 5437 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2243 3244 3626 4261 Size: 53 83 186 936 710 1001 382 635 Cuts at: 4261 275 Size: 1451 Fragments arranged by size: 1451 1001 936 710 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3870 4268 4542 4689 5100 3870 Size: 398 274 147 411 4207 Fragments arranged by size: 4207 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2435 2465 1178 Size: 30 165 30 1032 30 4150 Fragments arranged by size: 4150 1032 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2405 2531 1186 Size: 1219 126 4092 Fragments arranged by size: 4092 1219 126 BseYI C'CCAG_C Cuts at: 2037 2042 4640 5136 2037 Size: 5 2598 496 2338 Fragments arranged by size: 2598 2338 496 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4864 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2887 3648 4676 1540 Size: 275 186 886 761 1028 2301 Fragments arranged by size: 2301 1028 886 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3498 3583 4654 3498 Size: 85 1071 4281 Fragments arranged by size: 4281 1071 85 BsmI GAATG_Cn' Cuts at: 2948 3041 2948 Size: 93 5344 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2284 2388 2756 3498 3583 4654 1391 Size: 399 494 104 368 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 494 399 368 104 85 BspEI T'CCGG_A Cuts at: 5100 5100 Size: 5437 BspHI T'CATG_A Cuts at: 2290 3326 5056 2290 Size: 1036 1730 2671 Fragments arranged by size: 2671 1730 1036 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2277 2476 2792 844 Size: 1433 199 316 3489 Fragments arranged by size: 3489 1433 316 199 BsrBI CCG'CTC Cuts at: 1394 1823 3324 1394 Size: 429 1501 3507 Fragments arranged by size: 3507 1501 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2441 3932 4106 65 Size: 1183 1193 1491 174 1396 Fragments arranged by size: 1491 1396 1193 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2530 4081 4268 1700 Size: 110 720 1551 187 2869 Fragments arranged by size: 2869 1551 720 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5437 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5437 BssSI C'ACGA_G Cuts at: 2277 3497 4509 2277 Size: 1220 1012 3205 Fragments arranged by size: 3205 1220 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5437 BstBI TT'CG_AA Cuts at: 2719 4241 2719 Size: 1522 3915 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5437 BstYI r'GATC_y Cuts at: 1972 2730 2767 3134 3519 3536 4988 5074 5086 Size: 758 37 367 385 17 1452 86 12 Cuts at: 5086 5432 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 758 385 367 346 86 37 17 12 BstZ17I GTA'TAC Cuts at: 2339 2339 Size: 5437 BtgI C'CryG_G Cuts at: 513 1065 2004 2100 2323 2418 513 Size: 552 939 96 223 95 3532 Fragments arranged by size: 3532 939 552 223 96 95 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4698 Fragments arranged by size: 4698 683 56 BtsI GCAGTG_nn' Cuts at: 2953 3747 3767 2953 Size: 794 20 4623 Fragments arranged by size: 4623 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5437 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5402 DraI TTT'AAA Cuts at: 2741 2741 Size: 5437 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4444 809 Size: 507 3128 1802 Fragments arranged by size: 3128 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2208 2479 2564 Size: 54 1425 50 275 18 378 271 85 Cuts at: 2564 2664 2884 3773 5402 8 Size: 100 220 889 1629 43 Fragments arranged by size: 1629 1425 889 378 275 271 220 100 85 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2884 1537 Size: 275 1072 4090 Fragments arranged by size: 4090 1072 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3367 1353 Size: 2014 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4024 4398 4544 387 Size: 1064 138 2435 374 146 1280 Fragments arranged by size: 2435 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2462 2534 3500 4097 4884 5152 1239 Size: 393 830 72 966 597 787 268 1524 Fragments arranged by size: 1524 966 830 787 597 393 268 72 EcoNI CCTnn'n_nnAGG Cuts at: 2657 2657 Size: 5437 EcoO109I rG'GnC_Cy Cuts at: 2428 2466 2428 Size: 38 5399 EcoRI G'AATT_C Cuts at: 2746 3124 2746 Size: 378 5059 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2155 2155 Size: 5437 FspI TGC'GCA Cuts at: 2096 3943 2096 Size: 1847 3590 HaeII r_GCGC'y Cuts at: 1678 4584 1678 Size: 2906 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2018 2050 4078 4110 4152 Size: 32 135 32 446 32 2028 32 42 Cuts at: 4152 4184 1373 Size: 32 2626 Fragments arranged by size: 2626 2028 446 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2781 3027 669 Size: 988 281 843 246 3079 Fragments arranged by size: 3079 988 843 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5437 HpaI GTT'AAC Cuts at: 3027 3027 Size: 5437 KpnI G_GTAC'C Cuts at: 2762 2762 Size: 5437 MfeI C'AATT_G Cuts at: 3036 3036 Size: 5437 MluI A'CGCG_T Cuts at: 5313 5313 Size: 5437 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4551 4735 1305 Size: 3246 184 2007 Fragments arranged by size: 3246 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2481 10 Size: 54 1425 343 649 2966 Fragments arranged by size: 2966 1425 649 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3395 3754 3913 518 Size: 648 2229 359 159 2042 Fragments arranged by size: 2229 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2853 3530 4678 4923 1606 Size: 1247 677 1148 245 2120 Fragments arranged by size: 2120 1247 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2418 513 Size: 552 939 414 3532 Fragments arranged by size: 3532 939 552 414 NdeI CA'TA_TG Cuts at: 387 387 Size: 5437 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5437 NotI GC'GGCC_GC Cuts at: 2884 2884 Size: 5437 NspI r_CATG'y Cuts at: 1132 1229 2312 2795 4340 1132 Size: 97 1083 483 1545 2229 Fragments arranged by size: 2229 1545 1083 483 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5437 PciI A'CATG_T Cuts at: 4336 4336 Size: 5437 PflMI CCAn_nnn'nTGG Cuts at: 1108 2359 2372 1108 Size: 1251 13 4173 Fragments arranged by size: 4173 1251 13 PfoI T'CCnGG_A Cuts at: 2652 2652 Size: 5437 PmeI GTTT'AAAC Cuts at: 2741 2741 Size: 5437 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2435 2467 3506 3538 5019 5051 2435 Size: 32 1039 32 1481 32 2821 Fragments arranged by size: 2821 1481 1039 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5437 PsiI TTA'TAA Cuts at: 3007 3007 Size: 5437 PstI C_TGCA'G Cuts at: 830 2274 2789 830 Size: 1444 515 3478 Fragments arranged by size: 3478 1444 515 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5437 SalI G'TCGA_C Cuts at: 1936 2779 1936 Size: 843 4594 SbfI CC_TGCA'GG Cuts at: 2274 2789 2274 Size: 515 4922 ScaI AGT'ACT Cuts at: 3685 5397 3685 Size: 1712 3725 SexAI A'CCwGG_T Cuts at: 2028 2028 Size: 5437 SfcI C'TryA_G Cuts at: 826 1045 2270 2785 3920 4601 4792 826 Size: 219 1225 515 1135 681 191 1471 Fragments arranged by size: 1471 1225 1135 681 515 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5437 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5437 SmaI CCC'GGG Cuts at: 1872 2764 1872 Size: 892 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2174 2644 3541 4442 4704 820 Size: 197 926 231 470 897 901 262 1553 Fragments arranged by size: 1553 926 901 897 470 262 231 197 SnaBI TAC'GTA Cuts at: 493 3228 493 Size: 2735 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5437 SphI G_CATG'C Cuts at: 1132 2312 2795 1132 Size: 1180 483 3774 Fragments arranged by size: 3774 1180 483 SspI AAT'ATT Cuts at: 5 52 3361 5 Size: 47 3309 2081 Fragments arranged by size: 3309 2081 47 StuI AGG'CCT Cuts at: 1517 2699 1517 Size: 1182 4255 StyI C'CwwG_G Cuts at: 513 1065 2004 2418 2876 513 Size: 552 939 414 458 3074 Fragments arranged by size: 3074 939 552 458 414 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2213 2597 3464 3481 3634 3819 4264 Size: 1255 633 384 867 17 153 185 445 Cuts at: 4264 325 Size: 1498 Fragments arranged by size: 1498 1255 867 633 445 384 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3683 5395 96 Size: 274 80 33 51 1443 1706 1712 138 Fragments arranged by size: 1712 1706 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5397 5429 5397 Size: 32 5405 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5437 XbaI T'CTAG_A Cuts at: 2773 2773 Size: 5437 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5437 XmaI C'CCGG_G Cuts at: 1870 2762 1870 Size: 892 4545 XmnI GAAnn'nnTTC Cuts at: 3566 3566 Size: 5437 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3245 276 Size: 53 83 186 936 1711 2468 Fragments arranged by size: 2468 1711 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI