(Circular) (Six-base) MAPSORT of: pFN21AE0148.seq Check: 979 from: 1 to: 5383 pFN21AE0148 5383 bp With 182 enzymes: SgfI * October 20, 2009 10:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2637 2637 Size: 5383 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3193 278 Size: 53 83 186 936 1657 2468 Fragments arranged by size: 2468 1657 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2726 1937 Size: 789 4594 Acc65I G'GTAC_C Cuts at: 2704 2704 Size: 5383 AclI AA'CG_TT Cuts at: 1563 3510 3883 1563 Size: 1947 373 3063 Fragments arranged by size: 3063 1947 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3446 4830 5098 1632 Size: 1814 1384 268 1917 Fragments arranged by size: 1917 1814 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5186 AflIII A'CryG_T Cuts at: 1203 2589 4282 5259 1203 Size: 1386 1693 977 1327 Fragments arranged by size: 1693 1386 1327 977 AgeI A'CCGG_T Cuts at: 4214 4214 Size: 5383 AhdI GACnn_n'nnGTC Cuts at: 2047 4112 2047 Size: 2065 3318 AleI CACnn'nnGTG Cuts at: 1166 2318 1166 Size: 1152 4231 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5383 AlwNI CAG_nnn'CTG Cuts at: 3126 4698 3126 Size: 1572 3811 ApaI G_GGCC'C Cuts at: 2013 2654 2013 Size: 641 4742 ApaLI G'TGCA_C Cuts at: 3440 4596 3440 Size: 1156 4227 ApoI r'AATT_y Cuts at: 1493 2692 2918 3070 5179 1493 Size: 1199 226 152 2109 1697 Fragments arranged by size: 2109 1697 1199 226 152 AseI AT'TA_AT Cuts at: 160 1032 3937 160 Size: 872 2905 1606 Fragments arranged by size: 2905 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5383 AvaI C'yCGr_G Cuts at: 1870 1943 2358 2708 1870 Size: 73 415 350 4545 Fragments arranged by size: 4545 415 350 73 BamHI G'GATC_C Cuts at: 2713 3080 2713 Size: 367 5016 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2704 4159 618 Size: 325 217 627 917 1455 1842 Fragments arranged by size: 1842 1455 917 627 325 217 BanII G_rGCy'C Cuts at: 1391 2013 2034 2654 2702 1391 Size: 622 21 620 48 4072 Fragments arranged by size: 4072 622 620 48 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2400 928 Size: 971 501 3911 Fragments arranged by size: 3911 971 501 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3574 3608 1903 Size: 34 1637 34 3678 Fragments arranged by size: 3678 1637 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3275 4485 3275 Size: 1210 4173 BclI T'GATC_A Cuts at: 1548 1548 Size: 5383 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3994 136 Size: 107 122 71 631 605 2322 1525 Fragments arranged by size: 2322 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5378 5378 Size: 5383 BlpI GC'TnA_GC Cuts at: 2800 2800 Size: 5383 Bme1580I G_kGCm'C Cuts at: 1790 2013 2654 3444 4600 1790 Size: 223 641 790 1156 2573 Fragments arranged by size: 2573 1156 790 641 223 BmgBI CAC'GTC Cuts at: 1318 2044 1318 Size: 726 4657 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3114 4072 442 Size: 416 53 83 399 24 1697 958 1753 Fragments arranged by size: 1753 1697 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5383 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2154 2219 2366 2646 4043 1239 Size: 915 65 147 280 1397 2579 Fragments arranged by size: 2579 1397 915 280 147 65 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2492 3508 4373 4671 2492 Size: 1016 865 298 3204 Fragments arranged by size: 3204 1016 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2507 4046 882 Size: 617 1008 1539 2219 Fragments arranged by size: 2219 1539 1008 617 BsaAI yAC'GTr Cuts at: 493 1204 3174 493 Size: 711 1970 2702 Fragments arranged by size: 2702 1970 711 BsaBI GATnn'nnATC Cuts at: 2243 5377 2243 Size: 3134 2249 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3190 3572 4207 275 Size: 53 83 186 936 1657 382 635 1451 Fragments arranged by size: 1657 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2607 3816 4214 4488 4635 5046 2607 Size: 1209 398 274 147 411 2944 Fragments arranged by size: 2944 1209 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2217 2247 2339 2369 1178 Size: 30 165 30 814 30 92 30 4192 Fragments arranged by size: 4192 814 165 92 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5383 BseYI C'CCAG_C Cuts at: 2065 2126 2632 4586 5082 2065 Size: 61 506 1954 496 2366 Fragments arranged by size: 2366 1954 506 496 61 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4810 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2833 3594 4622 1540 Size: 275 186 832 761 1028 2301 Fragments arranged by size: 2301 1028 832 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2034 3444 3529 4600 2034 Size: 1410 85 1071 2817 Fragments arranged by size: 2817 1410 1071 85 BsmI GAATG_Cn' Cuts at: 2677 2894 2987 2677 Size: 217 93 5073 Fragments arranged by size: 5073 217 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2034 2654 2702 3444 3529 4600 Size: 399 223 21 620 48 742 85 1071 Cuts at: 4600 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 620 399 223 85 48 21 BspEI T'CCGG_A Cuts at: 5046 5046 Size: 5383 BspHI T'CATG_A Cuts at: 2145 3272 5002 2145 Size: 1127 1730 2526 Fragments arranged by size: 2526 1730 1127 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2121 2637 2738 844 Size: 1277 516 101 3489 Fragments arranged by size: 3489 1277 516 101 BsrBI CCG'CTC Cuts at: 1394 1823 3270 1394 Size: 429 1447 3507 Fragments arranged by size: 3507 1447 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3878 4052 65 Size: 1183 2630 174 1396 Fragments arranged by size: 2630 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2085 4027 4214 1700 Size: 110 275 1942 187 2869 Fragments arranged by size: 2869 1942 275 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5383 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5383 BssSI C'ACGA_G Cuts at: 3443 4455 3443 Size: 1012 4371 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5383 BstBI TT'CG_AA Cuts at: 4187 4187 Size: 5383 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5383 BstXI CCAn_nnnn'nTGG Cuts at: 2563 2576 2563 Size: 13 5370 BstYI r'GATC_y Cuts at: 1972 2076 2713 3080 3465 3482 4934 5020 5032 Size: 104 637 367 385 17 1452 86 12 Cuts at: 5032 5378 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 637 385 367 346 104 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2025 2562 513 Size: 552 939 21 537 3334 Fragments arranged by size: 3334 939 552 537 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4644 Fragments arranged by size: 4644 683 56 BtsI GCAGTG_nn' Cuts at: 2899 3693 3713 2899 Size: 794 20 4569 Fragments arranged by size: 4569 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5383 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5348 DraI TTT'AAA Cuts at: 2687 2687 Size: 5383 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4390 809 Size: 507 3074 1802 Fragments arranged by size: 3074 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2578 2830 3719 Size: 54 1425 50 275 18 748 252 889 Cuts at: 3719 5348 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 748 275 252 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2830 1537 Size: 275 1018 4090 Fragments arranged by size: 4090 1018 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2039 3313 1353 Size: 686 1274 3423 Fragments arranged by size: 3423 1274 686 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3970 4344 4490 387 Size: 1064 138 2381 374 146 1280 Fragments arranged by size: 2381 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2032 2032 Size: 5383 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2154 2219 2366 2646 3446 4043 4830 Size: 393 522 65 147 280 800 597 787 Cuts at: 4830 5098 1239 Size: 268 1524 Fragments arranged by size: 1524 800 787 597 522 393 280 268 147 65 EcoO109I rG'GnC_Cy Cuts at: 2009 2010 2651 2009 Size: 1 641 4741 Fragments arranged by size: 4741 641 1 EcoRI G'AATT_C Cuts at: 2692 3070 2692 Size: 378 5005 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2281 2281 Size: 5383 FspI TGC'GCA Cuts at: 3889 3889 Size: 5383 HaeII r_GCGC'y Cuts at: 1678 2489 4530 1678 Size: 811 2041 2531 Fragments arranged by size: 2531 2041 811 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2033 2065 2215 2217 2247 Size: 32 135 32 461 32 150 2 30 Cuts at: 2247 2249 2252 2284 2339 2371 4024 4056 4098 Size: 2 3 32 55 32 1653 32 42 Cuts at: 4098 4130 1373 Size: 32 2626 Fragments arranged by size: 2626 1653 461 150 135 55 42 32 32 32 32 32 32 32 30 3 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 2727 2973 669 Size: 988 281 789 246 3079 Fragments arranged by size: 3079 988 789 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5383 HpaI GTT'AAC Cuts at: 2973 2973 Size: 5383 KpnI G_GTAC'C Cuts at: 2708 2708 Size: 5383 MfeI C'AATT_G Cuts at: 2982 2982 Size: 5383 MluI A'CGCG_T Cuts at: 5259 5259 Size: 5383 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4497 4681 1305 Size: 3192 184 2007 Fragments arranged by size: 3192 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2580 10 Size: 54 1425 343 748 2813 Fragments arranged by size: 2813 1425 748 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2318 2561 2574 3341 3700 3859 518 Size: 648 1152 243 13 767 359 159 2042 Fragments arranged by size: 2042 1152 767 648 359 243 159 13 MspA1I CmG'CkG Cuts at: 1606 2799 3476 4624 4869 1606 Size: 1193 677 1148 245 2120 Fragments arranged by size: 2120 1193 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3892 Fragments arranged by size: 3892 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5383 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5383 NotI GC'GGCC_GC Cuts at: 2830 2830 Size: 5383 NspI r_CATG'y Cuts at: 1132 1229 2593 2741 4286 1132 Size: 97 1364 148 1545 2229 Fragments arranged by size: 2229 1545 1364 148 97 PasI CC'CwG_GG Cuts at: 1348 2461 1348 Size: 1113 4270 PciI A'CATG_T Cuts at: 2589 4282 2589 Size: 1693 3690 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5383 PmeI GTTT'AAAC Cuts at: 2687 2687 Size: 5383 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3452 3484 4965 4997 3452 Size: 32 1481 32 3838 Fragments arranged by size: 3838 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5383 PsiI TTA'TAA Cuts at: 2953 2953 Size: 5383 PspOMI G'GGCC_C Cuts at: 2009 2650 2009 Size: 641 4742 PstI C_TGCA'G Cuts at: 830 2118 2431 2452 2534 2735 830 Size: 1288 313 21 82 201 3478 Fragments arranged by size: 3478 1288 313 201 82 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5383 SacI G_AGCT'C Cuts at: 2034 2034 Size: 5383 SalI G'TCGA_C Cuts at: 1936 2725 1936 Size: 789 4594 SapI GCTCTTCn'nnn_ Cuts at: 2039 2039 Size: 5383 SbfI CC_TGCA'GG Cuts at: 2735 2735 Size: 5383 ScaI AGT'ACT Cuts at: 3631 5343 3631 Size: 1712 3671 SfcI C'TryA_G Cuts at: 826 1045 2114 2427 2448 2530 2731 3866 4547 Size: 219 1069 313 21 82 201 1135 681 Cuts at: 4547 4738 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1069 681 313 219 201 191 82 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5383 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5383 SmaI CCC'GGG Cuts at: 1872 2360 2710 1872 Size: 488 350 4545 Fragments arranged by size: 4545 488 350 SmlI C'TyrA_G Cuts at: 820 1017 1943 2507 3487 4388 4650 820 Size: 197 926 564 980 901 262 1553 Fragments arranged by size: 1553 980 926 901 564 262 197 SnaBI TAC'GTA Cuts at: 493 3174 493 Size: 2681 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5383 SphI G_CATG'C Cuts at: 1132 2741 1132 Size: 1609 3774 SspI AAT'ATT Cuts at: 5 52 3307 5 Size: 47 3255 2081 Fragments arranged by size: 3255 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5383 StyI C'CwwG_G Cuts at: 513 1065 2004 2256 2822 513 Size: 552 939 252 566 3074 Fragments arranged by size: 3074 939 566 552 252 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3410 3427 3580 3765 4210 325 Size: 1255 1830 17 153 185 445 1498 Fragments arranged by size: 1830 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3629 5341 96 Size: 274 80 33 51 1443 1652 1712 138 Fragments arranged by size: 1712 1652 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5343 5375 5343 Size: 32 5351 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5383 XbaI T'CTAG_A Cuts at: 2719 2719 Size: 5383 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2594 2594 Size: 5383 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5383 XmaI C'CCGG_G Cuts at: 1870 2358 2708 1870 Size: 488 350 4545 Fragments arranged by size: 4545 488 350 XmnI GAAnn'nnTTC Cuts at: 3512 3512 Size: 5383 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3191 276 Size: 53 83 186 936 1657 2468 Fragments arranged by size: 2468 1657 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI