Restriction map for
pFN21AE0369
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BamHI BanI BbeI BbrPI BbsI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HincII
HindII HindIII HinfI HpaI KasI
KpnI KspI MamI MfeI MroI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacII
SalI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI StuI SwaI TfiI XbaI
XhoI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AfaI 567 GTAC GT^AC
AflIII 853 ACGCGT A^C[GA][CT]GT
ApaI 14 GGGCCC GGGCC^C
Asp700I 534 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 139 GAGCTC G[AT]GC[AT]^C
AspI 1228 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 295 GGACC G^G[AT]CC
BsaI 821 GAGACC GGTCTC(1/5)
BsiEI 195 CGGTCG CG[GA][CT]^CG
BsiHKAI 139 GAGCTC G[AT]GC[AT]^C
BspHI 800 TCATGA T^CATGA
BssHII 311 GCGCGC G^CGCGC
BstXI 1044 CCAGAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 1360 TTTAAA TTT^AAA
DraIII 393 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 375 CCTCAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 363 CCCGC CCCGC(4/6)
HaeII 505 AGCGCC [GA]GCGC^[CT]
HgaI 441 GACGC GACGC(5/10)
MluI 853 ACGCGT A^CGCGT
MslI 916 CACCTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 91 CAGCGG C[AC]G^C[GT]G
PflFI 1228 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 381 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 294 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 800 TCATGA T^CATGA
RsaI 567 GTAC GT^AC
SacI 139 GAGCTC GAGCT^C
SbfI 48 CCTGCAGG CCTGCA^GG
ScaI 566 AGTACT AGT^ACT
SmlI 826 CTCAAG C^T[CT][GA]AG
Sse8387I 48 CCTGCAGG CCTGCA^GG
TaqI 542 TCGA T^CGA
Tth111I 1228 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 542 TCGA T^CGA
Van91I 381 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 534 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 1237 AAATTC [GA]^AATT[CT]
1486 GAATTT
ApoI 1237 AAATTC [GA]^AATT[CT]
1486 GAATTT
AvaI 118 CCCGGG C^[CT]CG[GA]G
354 CCCGGG
BanII 14 GGGCCC G[GA]GC[CT]^C
139 GAGCTC
BbvCI 375 CCTCAGC CCTCAGC(-5/-2)
454 GCTGAGG
BfaI 220 CTAG C^TAG
259 CTAG
BseRI 289 GAGGAG GAGGAG(10/8)
1618 GAGGAG
BsgI 913 CTGCAC GTGCAG(16/14)
268 GTGCAG
BsmAI 821 GAGAC GTCTC(1/5)
754 GTCTC
BsoBI 118 CCCGGG C^[CT]CG[GA]G
354 CCCGGG
BspMI 47 ACCTGC ACCTGC(4/8)
989 GCAGGT
BstYI 1079 AGATCC [GA]^GATC[CT]
1203 GGATCT
MaeI 220 CTAG C^TAG
259 CTAG
MflI 1079 AGATCC [GA]^GATC[CT]
1203 GGATCT
MseI 488 TTAA T^TAA
1361 TTAA
NcoI 461 CCATGG C^CATGG
1127 CCATGG
SapI 421 GAAGAGC GCTCTTC(1/4)
1284 GCTCTTC
SmaI 118 CCCGGG CCC^GGG
354 CCCGGG
Tru9I 488 TTAA T^TAA
1361 TTAA
XcmI 894 CCAGATAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1058 CCAATGGTTACATGG
XhoII 1079 AGATCC [GA]^GATC[CT]
1203 GGATCT
XmaCI 118 CCCGGG C^CCGGG
354 CCCGGG
XmaI 118 CCCGGG C^CCGGG
354 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
BalI BbvI BcnI BmyI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MluNI MnlI MscI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PstI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TseI Tsp45I
Tsp509I TspRI