(Circular) (Six-base) MAPSORT of: pFN21AE0369.seq Check: 4037 from: 1 to: 6364 pFN21AE0369 6364 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4174 278 Size: 53 83 186 936 2638 2468 Fragments arranged by size: 2638 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3707 1937 Size: 1770 4594 Acc65I G'GTAC_C Cuts at: 3685 3685 Size: 6364 AclI AA'CG_TT Cuts at: 1563 4491 4864 1563 Size: 2928 373 3063 Fragments arranged by size: 3063 2928 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2705 3233 3414 4427 5811 6079 1632 Size: 1073 528 181 1013 1384 268 1917 Fragments arranged by size: 1917 1384 1073 1013 528 268 181 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6167 AflIII A'CryG_T Cuts at: 1203 2858 5263 6240 1203 Size: 1655 2405 977 1327 Fragments arranged by size: 2405 1655 1327 977 AgeI A'CCGG_T Cuts at: 5195 5195 Size: 6364 AhdI GACnn_n'nnGTC Cuts at: 5093 5093 Size: 6364 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6364 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2108 1184 Size: 924 5440 AlwNI CAG_nnn'CTG Cuts at: 2158 2329 3539 4107 5679 2158 Size: 171 1210 568 1572 2843 Fragments arranged by size: 2843 1572 1210 568 171 ApaI G_GGCC'C Cuts at: 2023 2023 Size: 6364 ApaLI G'TGCA_C Cuts at: 4421 5577 4421 Size: 1156 5208 ApoI r'AATT_y Cuts at: 1493 3242 3491 3673 3899 4051 6160 1493 Size: 1749 249 182 226 152 2109 1697 Fragments arranged by size: 2109 1749 1697 249 226 182 152 AseI AT'TA_AT Cuts at: 160 1032 4918 160 Size: 872 3886 1606 Fragments arranged by size: 3886 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6364 AvaI C'yCGr_G Cuts at: 1870 1943 2123 2359 3689 1870 Size: 73 180 236 1330 4545 Fragments arranged by size: 4545 1330 236 180 73 BamHI G'GATC_C Cuts at: 3694 4061 3694 Size: 367 5997 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3685 5140 618 Size: 325 217 627 1898 1455 1842 Fragments arranged by size: 1898 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2023 2148 3683 1391 Size: 632 125 1535 4072 Fragments arranged by size: 4072 1535 632 125 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5393 BbvCI CC'TCA_GC Cuts at: 2381 2460 2381 Size: 79 6285 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4555 4589 1903 Size: 34 2618 34 3678 Fragments arranged by size: 3678 2618 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4256 5466 4256 Size: 1210 5154 BclI T'GATC_A Cuts at: 1548 1548 Size: 6364 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4975 136 Size: 107 122 71 631 605 3303 1525 Fragments arranged by size: 3303 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6359 6359 Size: 6364 BlpI GC'TnA_GC Cuts at: 3781 3781 Size: 6364 Bme1580I G_kGCm'C Cuts at: 1790 2023 2238 2353 2858 4425 5581 1790 Size: 233 215 115 505 1567 1156 2573 Fragments arranged by size: 2573 1567 1156 505 233 215 115 BmgBI CAC'GTC Cuts at: 1318 2272 1318 Size: 954 5410 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2290 2652 4095 Size: 416 53 83 399 24 873 362 1443 Cuts at: 4095 5053 442 Size: 958 1753 Fragments arranged by size: 1753 1443 958 873 416 399 362 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6364 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5024 1239 Size: 3785 2579 Bpu10I CC'TnA_GC Cuts at: 2381 2460 3319 2381 Size: 79 859 5426 Fragments arranged by size: 5426 859 79 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2816 4489 5354 5652 2816 Size: 1673 865 298 3528 Fragments arranged by size: 3528 1673 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2820 5027 882 Size: 617 1321 2207 2219 Fragments arranged by size: 2219 2207 1321 617 BsaAI yAC'GTr Cuts at: 493 1204 4155 493 Size: 711 2951 2702 Fragments arranged by size: 2951 2702 711 BsaBI GATnn'nnATC Cuts at: 6358 6358 Size: 6364 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4171 4553 5188 275 Size: 53 83 186 936 2638 382 635 1451 Fragments arranged by size: 2638 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4797 5195 5469 5616 6027 4797 Size: 398 274 147 411 5134 Fragments arranged by size: 5134 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6139 Fragments arranged by size: 6139 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2309 3638 1186 Size: 1123 1329 3912 Fragments arranged by size: 3912 1329 1123 BseYI C'CCAG_C Cuts at: 2593 5567 6063 2593 Size: 2974 496 2894 Fragments arranged by size: 2974 2894 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2294 2903 1163 Size: 573 558 609 4624 Fragments arranged by size: 4624 609 573 558 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2203 3814 4575 5603 1540 Size: 275 186 202 1611 761 1028 2301 Fragments arranged by size: 2301 1611 1028 761 275 202 186 BsiHKAI G_wGCw'C Cuts at: 2148 4425 4510 5581 2148 Size: 2277 85 1071 2931 Fragments arranged by size: 2931 2277 1071 85 BsmI GAATG_Cn' Cuts at: 3875 3968 3875 Size: 93 6271 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2023 2148 2238 2353 2858 3683 4425 Size: 399 233 125 90 115 505 825 742 Cuts at: 4425 4510 5581 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 825 742 505 399 233 125 115 90 85 BspEI T'CCGG_A Cuts at: 6027 6027 Size: 6364 BspHI T'CATG_A Cuts at: 2805 4253 5983 2805 Size: 1448 1730 3186 Fragments arranged by size: 3186 1730 1448 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2061 2985 3719 844 Size: 1217 924 734 3489 Fragments arranged by size: 3489 1217 924 734 BsrBI CCG'CTC Cuts at: 1394 1823 4251 1394 Size: 429 2428 3507 Fragments arranged by size: 3507 2428 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4859 5033 65 Size: 1183 3611 174 1396 Fragments arranged by size: 3611 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5008 5195 1700 Size: 110 3198 187 2869 Fragments arranged by size: 3198 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6364 BssHII G'CGCG_C Cuts at: 1925 2316 1925 Size: 391 5973 BssSI C'ACGA_G Cuts at: 4424 5436 4424 Size: 1012 5352 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6364 BstBI TT'CG_AA Cuts at: 5168 5168 Size: 6364 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6364 BstXI CCAn_nnnn'nTGG Cuts at: 3056 3056 Size: 6364 BstYI r'GATC_y Cuts at: 1972 3084 3208 3694 4061 4446 4463 5915 6001 Size: 1112 124 486 367 385 17 1452 86 Cuts at: 6001 6013 6359 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1112 486 385 367 346 124 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2391 2466 3018 3132 513 Size: 552 939 387 75 552 114 3745 Fragments arranged by size: 3745 939 552 552 387 114 75 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3436 487 Size: 683 56 2210 3415 Fragments arranged by size: 3415 2210 683 56 BtsI GCAGTG_nn' Cuts at: 2448 2540 3880 4674 4694 2448 Size: 92 1340 794 20 4118 Fragments arranged by size: 4118 1340 794 92 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6364 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6329 DraI TTT'AAA Cuts at: 3367 3668 3367 Size: 301 6063 DraIII CAC_nnn'GTG Cuts at: 2403 2403 Size: 6364 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5371 809 Size: 507 4055 1802 Fragments arranged by size: 4055 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2289 2334 2394 Size: 54 1425 50 275 18 459 45 60 Cuts at: 2394 2451 3015 3811 4700 6329 8 Size: 57 564 796 889 1629 43 Fragments arranged by size: 1629 1425 889 796 564 459 275 60 57 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3811 1537 Size: 275 1999 4090 Fragments arranged by size: 4090 1999 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2216 2421 2549 3296 4294 1353 Size: 863 205 128 747 998 3423 Fragments arranged by size: 3423 998 863 747 205 128 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2138 4951 5325 5471 387 Size: 1064 138 549 2813 374 146 1280 Fragments arranged by size: 2813 1280 1064 549 374 146 138 EcoICRI GAG'CTC Cuts at: 2146 2146 Size: 6364 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2705 3233 3414 4427 5024 5811 6079 Size: 393 1073 528 181 1013 597 787 268 Cuts at: 6079 1239 Size: 1524 Fragments arranged by size: 1524 1073 1013 787 597 528 393 268 181 EcoNI CCTnn'n_nnAGG Cuts at: 2384 2384 Size: 6364 EcoO109I rG'GnC_Cy Cuts at: 2019 2020 2300 2019 Size: 1 280 6083 Fragments arranged by size: 6083 280 1 EcoRI G'AATT_C Cuts at: 3673 4051 3673 Size: 378 5986 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2746 3532 2746 Size: 786 5578 FspI TGC'GCA Cuts at: 4870 4870 Size: 6364 HaeII r_GCGC'y Cuts at: 1678 2514 5511 1678 Size: 836 2997 2531 Fragments arranged by size: 2997 2531 836 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3455 3487 5005 5037 5079 Size: 32 135 32 1883 32 1518 32 42 Cuts at: 5079 5111 1373 Size: 32 2626 Fragments arranged by size: 2626 1883 1518 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3708 3954 669 Size: 988 281 1770 246 3079 Fragments arranged by size: 3079 1770 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6364 HpaI GTT'AAC Cuts at: 3954 3954 Size: 6364 KpnI G_GTAC'C Cuts at: 3689 3689 Size: 6364 MfeI C'AATT_G Cuts at: 3963 3963 Size: 6364 MluI A'CGCG_T Cuts at: 2858 6240 2858 Size: 3382 2982 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5478 5662 1305 Size: 4173 184 2007 Fragments arranged by size: 4173 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2336 2396 2453 10 Size: 54 1425 343 504 60 57 3921 Fragments arranged by size: 3921 1425 504 343 60 57 54 MslI CAynn'nnrTG Cuts at: 518 1166 2925 4322 4681 4840 518 Size: 648 1759 1397 359 159 2042 Fragments arranged by size: 2042 1759 1397 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2098 3780 4457 5605 5850 1606 Size: 492 1682 677 1148 245 2120 Fragments arranged by size: 2120 1682 1148 677 492 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2466 3132 513 Size: 552 939 462 666 3745 Fragments arranged by size: 3745 939 666 552 462 NdeI CA'TA_TG Cuts at: 387 387 Size: 6364 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6364 NotI GC'GGCC_GC Cuts at: 3811 3811 Size: 6364 NspI r_CATG'y Cuts at: 1132 1229 3722 5267 1132 Size: 97 2493 1545 2229 Fragments arranged by size: 2493 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6364 PciI A'CATG_T Cuts at: 5263 5263 Size: 6364 PflMI CCAn_nnn'nTGG Cuts at: 1108 2392 1108 Size: 1284 5080 PmeI GTTT'AAAC Cuts at: 3668 3668 Size: 6364 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4433 4465 5946 5978 4433 Size: 32 1481 32 4819 Fragments arranged by size: 4819 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2300 2300 Size: 6364 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6364 PsiI TTA'TAA Cuts at: 3934 3934 Size: 6364 PspOMI G'GGCC_C Cuts at: 2019 2019 Size: 6364 PstI C_TGCA'G Cuts at: 830 2058 2097 2133 2325 2443 2535 2996 3716 Size: 1228 39 36 192 118 92 461 720 Cuts at: 3716 830 Size: 3478 Fragments arranged by size: 3478 1228 720 461 192 118 92 39 36 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6364 SacI G_AGCT'C Cuts at: 2148 2148 Size: 6364 SalI G'TCGA_C Cuts at: 1936 3706 1936 Size: 1770 4594 SanDI GG'GwC_CC Cuts at: 2300 2300 Size: 6364 SapI GCTCTTCn'nnn_ Cuts at: 2421 3296 2421 Size: 875 5489 SbfI CC_TGCA'GG Cuts at: 2058 3716 2058 Size: 1658 4706 ScaI AGT'ACT Cuts at: 2573 4612 6324 2573 Size: 2039 1712 2613 Fragments arranged by size: 2613 2039 1712 SfcI C'TryA_G Cuts at: 826 1045 2054 2093 2129 2321 2439 2531 2992 Size: 219 1009 39 36 192 118 92 461 Cuts at: 2992 3546 3712 4847 5528 5719 826 Size: 554 166 1135 681 191 1471 Fragments arranged by size: 1471 1135 1009 681 554 461 219 192 191 166 118 92 39 36 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6364 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6364 SmaI CCC'GGG Cuts at: 1872 2125 2361 3691 1872 Size: 253 236 1330 4545 Fragments arranged by size: 4545 1330 253 236 SmlI C'TyrA_G Cuts at: 820 1017 1943 2831 4468 5369 5631 820 Size: 197 926 888 1637 901 262 1553 Fragments arranged by size: 1637 1553 926 901 888 262 197 SnaBI TAC'GTA Cuts at: 493 4155 493 Size: 3662 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6364 SphI G_CATG'C Cuts at: 1132 3722 1132 Size: 2590 3774 SrfI GCCC'GGGC Cuts at: 2125 2125 Size: 6364 SspI AAT'ATT Cuts at: 5 52 4288 5 Size: 47 4236 2081 Fragments arranged by size: 4236 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6364 StyI C'CwwG_G Cuts at: 513 1065 2004 2454 2466 3132 3803 513 Size: 552 939 450 12 666 671 3074 Fragments arranged by size: 3074 939 671 666 552 450 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2189 3062 4391 4408 4561 4746 5191 Size: 1255 609 873 1329 17 153 185 445 Cuts at: 5191 325 Size: 1498 Fragments arranged by size: 1498 1329 1255 873 609 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2571 4610 6322 Size: 274 80 33 51 1443 594 2039 1712 Cuts at: 6322 96 Size: 138 Fragments arranged by size: 2039 1712 1443 594 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6324 6356 6324 Size: 32 6332 Tth111I GACn'n_nGTC Cuts at: 1316 3236 1316 Size: 1920 4444 XbaI T'CTAG_A Cuts at: 3700 3700 Size: 6364 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2906 3070 2011 Size: 895 164 5305 Fragments arranged by size: 5305 895 164 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6364 XmaI C'CCGG_G Cuts at: 1870 2123 2359 3689 1870 Size: 253 236 1330 4545 Fragments arranged by size: 4545 1330 253 236 XmnI GAAnn'nnTTC Cuts at: 2543 4493 2543 Size: 1950 4414 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4172 276 Size: 53 83 186 936 2638 2468 Fragments arranged by size: 2638 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII SexAI SfiI SfoI SwaI