Restriction map for
pFN21AE0369
(Linear) (Six-base) MAPSORT of: KIEE0369.seq Check: 9753 from: 1 to: 1659
KIEE0369.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 700 1228 1409 1659
Size: 700 528 181 250
Fragments arranged by size:
700 528 250 181
AflIII A'CryG_T
Cuts at: 0 853 1659
Size: 853 806
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 103 1659
Size: 103 1556
AlwNI CAG_nnn'CTG
Cuts at: 0 153 324 1534 1659
Size: 153 171 1210 125
Fragments arranged by size:
1210 171 153 125
ApaI G_GGCC'C
Cuts at: 0 18 1659
Size: 18 1641
ApoI r'AATT_y
Cuts at: 0 1237 1486 1659
Size: 1237 249 173
Fragments arranged by size:
1237 249 173
AvaI C'yCGr_G
Cuts at: 0 118 354 1659
Size: 118 236 1305
Fragments arranged by size:
1305 236 118
BanII G_rGCy'C
Cuts at: 0 18 143 1659
Size: 18 125 1516
Fragments arranged by size:
1516 125 18
BbvCI CC'TCA_GC
Cuts at: 0 376 455 1659
Size: 376 79 1204
Fragments arranged by size:
1204 376 79
Bme1580I G_kGCm'C
Cuts at: 0 18 233 348 853 1659
Size: 18 215 115 505 806
Fragments arranged by size:
806 505 215 115 18
BmgBI CAC'GTC
Cuts at: 0 267 1659
Size: 267 1392
BmrI ACTGGGnnnn_n'
Cuts at: 0 285 647 1659
Size: 285 362 1012
Fragments arranged by size:
1012 362 285
Bpu10I CC'TnA_GC
Cuts at: 0 376 455 1314 1659
Size: 376 79 859 345
Fragments arranged by size:
859 376 345 79
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 811 1659
Size: 811 848
BsaI GGTCTCn'nnnn_
Cuts at: 0 815 1659
Size: 815 844
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 304 1633 1659
Size: 304 1329 26
Fragments arranged by size:
1329 304 26
BseYI C'CCAG_C
Cuts at: 0 588 1659
Size: 588 1071
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 898 1659
Size: 289 609 761
Fragments arranged by size:
761 609 289
BsiEI CG_ry'CG
Cuts at: 0 198 1659
Size: 198 1461
BsiHKAI G_wGCw'C
Cuts at: 0 143 1659
Size: 143 1516
Bsp1286I G_dGCh'C
Cuts at: 0 18 143 233 348 853 1659
Size: 18 125 90 115 505 806
Fragments arranged by size:
806 505 125 115 90 18
BspHI T'CATG_A
Cuts at: 0 800 1659
Size: 800 859
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 56 980 1659
Size: 56 924 679
Fragments arranged by size:
924 679 56
BssHII G'CGCG_C
Cuts at: 0 311 1659
Size: 311 1348
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1051 1659
Size: 1051 608
BstYI r'GATC_y
Cuts at: 0 1079 1203 1659
Size: 1079 124 456
Fragments arranged by size:
1079 456 124
BtgI C'CryG_G
Cuts at: 0 386 461 1013 1127 1659
Size: 386 75 552 114 532
Fragments arranged by size:
552 532 386 114 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1431 1659
Size: 1431 228
BtsI GCAGTG_nn'
Cuts at: 0 443 535 1659
Size: 443 92 1124
Fragments arranged by size:
1124 443 92
DraI TTT'AAA
Cuts at: 0 1362 1659
Size: 1362 297
DraIII CAC_nnn'GTG
Cuts at: 0 398 1659
Size: 398 1261
EaeI y'GGCC_r
Cuts at: 0 284 329 389 446 1010 1659
Size: 284 45 60 57 564 649
Fragments arranged by size:
649 564 284 60 57 45
EarI CTCTTCn'nnn_
Cuts at: 0 211 416 544 1291 1659
Size: 211 205 128 747 368
Fragments arranged by size:
747 368 211 205 128
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 133 1659
Size: 133 1526
EcoICRI GAG'CTC
Cuts at: 0 141 1659
Size: 141 1518
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 700 1228 1409 1659
Size: 700 528 181 250
Fragments arranged by size:
700 528 250 181
EcoNI CCTnn'n_nnAGG
Cuts at: 0 379 1659
Size: 379 1280
EcoO109I rG'GnC_Cy
Cuts at: 0 14 15 295 1659
Size: 14 1 280 1364
Fragments arranged by size:
1364 280 14 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 741 1527 1659
Size: 741 786 132
Fragments arranged by size:
786 741 132
HaeII r_GCGC'y
Cuts at: 0 509 1659
Size: 509 1150
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1450 1482 1659
Size: 1450 32 177
Fragments arranged by size:
1450 177 32
MluI A'CGCG_T
Cuts at: 0 853 1659
Size: 853 806
MscI TGG'CCA
Cuts at: 0 331 391 448 1659
Size: 331 60 57 1211
Fragments arranged by size:
1211 331 60 57
MslI CAynn'nnrTG
Cuts at: 0 920 1659
Size: 920 739
MspA1I CmG'CkG
Cuts at: 0 93 1659
Size: 93 1566
NcoI C'CATG_G
Cuts at: 0 461 1127 1659
Size: 461 666 532
Fragments arranged by size:
666 532 461
PflMI CCAn_nnn'nTGG
Cuts at: 0 387 1659
Size: 387 1272
PpuMI rG'GwC_Cy
Cuts at: 0 295 1659
Size: 295 1364
PspOMI G'GGCC_C
Cuts at: 0 14 1659
Size: 14 1645
PstI C_TGCA'G
Cuts at: 0 53 92 128 320 438 530 991 1659
Size: 53 39 36 192 118 92 461 668
Fragments arranged by size:
668 461 192 118 92 53 39 36
SacI G_AGCT'C
Cuts at: 0 143 1659
Size: 143 1516
SanDI GG'GwC_CC
Cuts at: 0 295 1659
Size: 295 1364
SapI GCTCTTCn'nnn_
Cuts at: 0 416 1291 1659
Size: 416 875 368
Fragments arranged by size:
875 416 368
SbfI CC_TGCA'GG
Cuts at: 0 53 1659
Size: 53 1606
ScaI AGT'ACT
Cuts at: 0 568 1659
Size: 568 1091
SfcI C'TryA_G
Cuts at: 0 49 88 124 316 434 526 987 1541
Size: 49 39 36 192 118 92 461 554
Cuts at: 1541 1659
Size: 118
Fragments arranged by size:
554 461 192 118 118 92 49 39
36
SmaI CCC'GGG
Cuts at: 0 120 356 1659
Size: 120 236 1303
Fragments arranged by size:
1303 236 120
SmlI C'TyrA_G
Cuts at: 0 826 1659
Size: 826 833
SrfI GCCC'GGGC
Cuts at: 0 120 1659
Size: 120 1539
StyI C'CwwG_G
Cuts at: 0 449 461 1127 1659
Size: 449 12 666 532
Fragments arranged by size:
666 532 449 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 184 1057 1659
Size: 184 873 602
Fragments arranged by size:
873 602 184
TatI w'GTAC_w
Cuts at: 0 566 1659
Size: 566 1093
Tth111I GACn'n_nGTC
Cuts at: 0 1231 1659
Size: 1231 428
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 901 1065 1659
Size: 901 164 594
Fragments arranged by size:
901 594 164
XmaI C'CCGG_G
Cuts at: 0 118 354 1659
Size: 118 236 1305
Fragments arranged by size:
1305 236 118
XmnI GAAnn'nnTTC
Cuts at: 0 538 1659
Size: 538 1121
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApaI ApoI AvaI BanII
BbvCI Bme1580I BmgBI BmrI Bpu10I BpuEI BsaI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BssHII
BstXI BstYI BtgI BtgZI BtsI DraI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I MluI MscI MslI MspA1I NcoI PflMI PpuMI
PspOMI PstI SacI SanDI SapI SbfI ScaI SfcI
SmaI SmlI SrfI StyI TaqII TatI Tth111I XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmtI BplI BpmI BsaAI
BsaBI BsaHI BsaWI BsaXI BsiWI BsmI BsmBI BspEI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I ClaI CspCI DrdI EagI EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacII SalI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SspI StuI SwaI TstI XbaI
XhoI ZraI