################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AatII AccIII AclI AcsI AflII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI AspEI AvrII BamHI BciVI BfrI BlnI BsaI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI BstAPI BstBI BstEII BstPI Bsu36I ClaI CpoI DraI DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoT22I FseI MfeI MluI MroI MunI NheI NotI NruI NsiI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI ScaI SfuI SgrAI SnaBI SpeI SspBI SspI SwaI Tsp509I XbaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 1172 GGTACC G^GTACC Asp700I 2343 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1172 GGTACC G^GTACC BbrPI 1711 CACGTG CAC^GTG BbvCI 2013 GCTGAGG CCTCAGC(-5/-2) BcgI 672 CGAGGACGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1922 TGATCA T^GATCA BglII 758 AGATCT A^GATCT BsaBI 2490 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1034 ACCGGA [AT]^CCGG[AT] BsgI 1305 CTGCAC GTGCAG(16/14) BspHI 1058 TCATGA T^CATGA Bst1107I 691 GTATAC GTA^TAC BstZ17I 691 GTATAC GTA^TAC EarI 958 CTCTTC CTCTTC(1/4) EcoNI 885 CCTCGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 2068 GATATC GAT^ATC FbaI 1922 TGATCA T^GATCA HindIII 1875 AAGCTT A^AGCTT HpaI 2563 GTTAAC GTT^AAC KpnI 1172 GGTACC GGTAC^C Ksp632I 958 CTCTTC CTCTTC(1/4) KspI 913 CCGCGG CCGC^GG MamI 2490 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 2564 TTAA T^TAA NcoI 2709 CCATGG C^CATGG NdeI 2113 CATATG CA^TATG PmaCI 1711 CACGTG CAC^GTG PmlI 1711 CACGTG CAC^GTG PpuMI 239 AGGACCT [GA]G^G[AT]CC[CT] RcaI 1058 TCATGA T^CATGA SacII 913 CCGCGG CCGC^GG SapI 957 GCTCTTC GCTCTTC(1/4) SbfI 2583 CCTGCAGG CCTGCA^GG SfiI 2148 GGCCATCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 647 CCCGGG CCC^GGG Sse8387I 2583 CCTGCAGG CCTGCA^GG Tru9I 2564 TTAA T^TAA XcmI 1919 CCATGATCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 647 CCCGGG C^CCGGG XmaI 647 CCCGGG C^CCGGG XmnI 2343 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AlwI 1206 GATCC GGATC(4/5) 2480 GGATC AlwNI 1606 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG 2295 CAGCCCCTG ApaI 1312 GGGCCC GGGCC^C 2624 GGGCCC AspI 1285 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1803 GACCCTGTC AviII 413 TGCGCA TGC^GCA 2285 TGCGCA BfaI 271 CTAG C^TAG 2702 CTAG BsaAI 733 TACGTG [CT]AC^GT[GA] 1711 CACGTG BsiEI 1758 CGGCCG CG[GA][CT]^CG 1975 CGGCCG BsmBI 2313 CGTCTC CGTCTC(1/5) 541 GAGACG BstXI 212 CCATCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2337 CCACGAGAATGG BstYI 758 AGATCT [GA]^GATC[CT] 2480 GGATCT EagI 1758 CGGCCG C^GGCCG 1975 CGGCCG EclXI 1758 CGGCCG C^GGCCG 1975 CGGCCG Eco52I 1758 CGGCCG C^GGCCG 1975 CGGCCG EcoT14I 566 CCAAGG C^C[AT][AT]GG 2709 CCATGG FspI 413 TGCGCA TGC^GCA 2285 TGCGCA MaeI 271 CTAG C^TAG 2702 CTAG MflI 758 AGATCT [GA]^GATC[CT] 2480 GGATCT NaeI 331 GCCGGC GCC^GGC 1909 GCCGGC NgoMIV 331 GCCGGC G^CCGGC 1909 GCCGGC PaeR7I 886 CTCGAG C^TCGAG 1166 CTCGAG PflFI 1285 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1803 GACCCTGTC PflMI 443 CCAGCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 935 CCAACATCTGG PvuII 474 CAGCTG CAG^CTG 2545 CAGCTG SacI 880 GAGCTC GAGCT^C 2058 GAGCTC SexAI 1430 ACCTGGT A^CC[AT]GGT 2234 ACCTGGT SphI 2178 GCATGC GCATG^C 2282 GCATGC StuI 2216 AGGCCT AGG^CCT 2610 AGGCCT StyI 566 CCAAGG C^C[AT][AT]GG 2709 CCATGG TfiI 171 GATTC G^A[AT]TC 1635 GATTC Tth111I 1285 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1803 GACCCTGTC Van91I 443 CCAGCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 935 CCAACATCTGG XhoI 886 CTCGAG C^TCGAG 1166 CTCGAG XhoII 758 AGATCT [GA]^GATC[CT] 2480 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AflIII AluI Alw44I ApaLI AspHI AvaI AvaII BalI BanI BanII BbeI BbsI BbvI BcnI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssHII BssKI BssSI BstF5I BstNI BstUI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI TaqI TseI Tsp45I TspRI TthHB8I