(Linear) (Six-base) MAPSORT of: KIBB3278.seq Check: 1698 from: 1 to: 2727 KIBB3278.orf With 182 enzymes: SgfI * October 13, 2009 15:05 .. AccI GT'mk_AC Cuts at: 0 260 470 692 2727 Size: 260 210 222 2035 Fragments arranged by size: 2035 260 222 210 Acc65I G'GTAC_C Cuts at: 0 1172 2727 Size: 1172 1555 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1213 1731 2727 Size: 1213 518 996 Fragments arranged by size: 1213 996 518 AflIII A'CryG_T Cuts at: 0 734 1322 2567 2727 Size: 734 588 1245 160 Fragments arranged by size: 1245 734 588 160 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 367 1099 1399 2299 2540 2727 Size: 367 732 300 900 241 187 Fragments arranged by size: 900 732 367 300 241 187 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1024 1056 2727 Size: 1024 32 1671 Fragments arranged by size: 1671 1024 32 AlwNI CAG_nnn'CTG Cuts at: 0 1611 2300 2727 Size: 1611 689 427 Fragments arranged by size: 1611 689 427 ApaI G_GGCC'C Cuts at: 0 1316 2628 2727 Size: 1316 1312 99 Fragments arranged by size: 1316 1312 99 ApaLI G'TGCA_C Cuts at: 0 874 1426 1708 2727 Size: 874 552 282 1019 Fragments arranged by size: 1019 874 552 282 AvaI C'yCGr_G Cuts at: 0 647 762 799 886 1166 2265 2727 Size: 647 115 37 87 280 1099 462 Fragments arranged by size: 1099 647 462 280 115 87 37 BanI G'GyrC_C Cuts at: 0 1105 1172 1350 1542 1864 2081 2616 2727 Size: 1105 67 178 192 322 217 535 111 Fragments arranged by size: 1105 535 322 217 192 178 111 67 BanII G_rGCy'C Cuts at: 0 727 884 1220 1316 2062 2628 2727 Size: 727 157 336 96 746 566 99 Fragments arranged by size: 746 727 566 336 157 99 96 BbeI G_GCGC'C Cuts at: 0 1546 1868 2085 2727 Size: 1546 322 217 642 Fragments arranged by size: 1546 642 322 217 BbsI GAAGACnn'nnnn_ Cuts at: 0 952 971 2363 2439 2727 Size: 952 19 1392 76 288 Fragments arranged by size: 1392 952 288 76 19 BbvCI CC'TCA_GC Cuts at: 0 2014 2727 Size: 2014 713 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 661 695 2727 Size: 661 34 2032 Fragments arranged by size: 2032 661 34 BclI T'GATC_A Cuts at: 0 1922 2727 Size: 1922 805 BglI GCCn_nnn'nGGC Cuts at: 0 1757 1951 2155 2727 Size: 1757 194 204 572 Fragments arranged by size: 1757 572 204 194 BglII A'GATC_T Cuts at: 0 758 2727 Size: 758 1969 BlpI GC'TnA_GC Cuts at: 0 97 1516 2095 2727 Size: 97 1419 579 632 Fragments arranged by size: 1419 632 579 97 Bme1580I G_kGCm'C Cuts at: 0 878 1316 1430 1712 2621 2628 2727 Size: 878 438 114 282 909 7 99 Fragments arranged by size: 909 878 438 282 114 99 7 BmgBI CAC'GTC Cuts at: 0 1113 1325 2372 2727 Size: 1113 212 1047 355 Fragments arranged by size: 1113 1047 355 212 BmrI ACTGGGnnnn_n' Cuts at: 0 1282 2727 Size: 1282 1445 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 234 559 571 661 2227 2267 2717 2727 Size: 234 325 12 90 1566 40 450 10 Fragments arranged by size: 1566 450 325 234 90 40 12 10 Bpu10I CC'TnA_GC Cuts at: 0 403 2014 2727 Size: 403 1611 713 Fragments arranged by size: 1611 713 403 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2314 2727 Size: 2314 413 BsaAI yAC'GTr Cuts at: 0 735 1713 2727 Size: 735 978 1014 Fragments arranged by size: 1014 978 735 BsaBI GATnn'nnATC Cuts at: 0 2494 2727 Size: 2494 233 BsaHI Gr'CG_yC Cuts at: 0 848 1543 1835 1865 2082 2727 Size: 848 695 292 30 217 645 Fragments arranged by size: 848 695 645 292 217 30 BsaWI w'CCGG_w Cuts at: 0 1034 2727 Size: 1034 1693 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 232 262 547 577 2727 Size: 232 30 285 30 2150 Fragments arranged by size: 2150 285 232 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 568 874 1279 1795 1798 1801 1804 1996 Size: 568 306 405 516 3 3 3 192 Cuts at: 1996 2636 2727 Size: 640 91 Fragments arranged by size: 640 568 516 405 306 192 91 3 3 3 BseYI C'CCAG_C Cuts at: 0 354 2461 2727 Size: 354 2107 266 Fragments arranged by size: 2107 354 266 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1290 2727 Size: 1290 1437 BsiEI CG_ry'CG Cuts at: 0 1761 1978 2727 Size: 1761 217 749 Fragments arranged by size: 1761 749 217 BsiHKAI G_wGCw'C Cuts at: 0 7 103 878 884 1430 1648 1681 1712 Size: 7 96 775 6 546 218 33 31 Cuts at: 1712 2062 2101 2727 Size: 350 39 626 Fragments arranged by size: 775 626 546 350 218 96 39 33 31 7 6 BsmBI CGTCTCn'nnnn_ Cuts at: 0 535 2319 2727 Size: 535 1784 408 Fragments arranged by size: 1784 535 408 Bsp1286I G_dGCh'C Cuts at: 0 7 103 727 878 884 1220 1316 1430 Size: 7 96 624 151 6 336 96 114 Cuts at: 1430 1648 1681 1712 2062 2101 2621 2628 2727 Size: 218 33 31 350 39 520 7 99 Fragments arranged by size: 624 520 350 336 218 151 114 99 96 96 39 33 31 7 7 6 BspHI T'CATG_A Cuts at: 0 1058 2727 Size: 1058 1669 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1073 1391 1742 2577 2591 2727 Size: 1073 318 351 835 14 136 Fragments arranged by size: 1073 835 351 318 136 14 BsrBI CCG'CTC Cuts at: 0 957 1464 2264 2727 Size: 957 507 800 463 Fragments arranged by size: 957 800 507 463 BsrFI r'CCGG_y Cuts at: 0 331 1760 1909 1973 2108 2727 Size: 331 1429 149 64 135 619 Fragments arranged by size: 1429 619 331 149 135 64 BssHII G'CGCG_C Cuts at: 0 1566 1568 1885 2727 Size: 1566 2 317 842 Fragments arranged by size: 1566 842 317 2 BssSI C'ACGA_G Cuts at: 0 112 511 877 2338 2727 Size: 112 399 366 1461 389 Fragments arranged by size: 1461 399 389 366 112 BstXI CCAn_nnnn'nTGG Cuts at: 0 219 2344 2727 Size: 219 2125 383 Fragments arranged by size: 2125 383 219 BstYI r'GATC_y Cuts at: 0 758 2480 2727 Size: 758 1722 247 Fragments arranged by size: 1722 758 247 BstZ17I GTA'TAC Cuts at: 0 693 2727 Size: 693 2034 BtgI C'CryG_G Cuts at: 0 913 2045 2063 2637 2663 2709 2727 Size: 913 1132 18 574 26 46 18 Fragments arranged by size: 1132 913 574 46 26 18 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 721 2727 Size: 721 2006 DraIII CAC_nnn'GTG Cuts at: 0 1350 1434 1685 1716 2727 Size: 1350 84 251 31 1011 Fragments arranged by size: 1350 1011 251 84 31 EaeI y'GGCC_r Cuts at: 0 1141 1718 1758 1814 1975 2438 2474 2634 Size: 1141 577 40 56 161 463 36 160 Cuts at: 2634 2712 2727 Size: 78 15 Fragments arranged by size: 1141 577 463 161 160 78 56 40 36 15 EagI C'GGCC_G Cuts at: 0 1758 1975 2727 Size: 1758 217 752 Fragments arranged by size: 1758 752 217 EarI CTCTTCn'nnn_ Cuts at: 0 964 2727 Size: 964 1763 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1163 2158 2589 2727 Size: 1163 995 431 138 Fragments arranged by size: 1163 995 431 138 EcoICRI GAG'CTC Cuts at: 0 882 2060 2727 Size: 882 1178 667 Fragments arranged by size: 1178 882 667 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 234 559 571 661 1213 1731 2227 2267 Size: 234 325 12 90 552 518 496 40 Cuts at: 2267 2717 2727 Size: 450 10 Fragments arranged by size: 552 518 496 450 325 234 90 40 12 10 EcoNI CCTnn'n_nnAGG Cuts at: 0 889 2727 Size: 889 1838 EcoO109I rG'GnC_Cy Cuts at: 0 240 1608 2624 2727 Size: 240 1368 1016 103 Fragments arranged by size: 1368 1016 240 103 EcoRV GAT'ATC Cuts at: 0 2070 2727 Size: 2070 657 FspI TGC'GCA Cuts at: 0 415 2287 2727 Size: 415 1872 440 Fragments arranged by size: 1872 440 415 HaeII r_GCGC'y Cuts at: 0 1546 1868 2085 2727 Size: 1546 322 217 642 Fragments arranged by size: 1546 642 322 217 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 232 264 547 579 2727 Size: 232 32 283 32 2148 Fragments arranged by size: 2148 283 232 32 32 HincII GTy'rAC Cuts at: 0 81 1278 2565 2727 Size: 81 1197 1287 162 Fragments arranged by size: 1287 1197 162 81 HindIII A'AGCT_T Cuts at: 0 1875 2727 Size: 1875 852 HpaI GTT'AAC Cuts at: 0 2565 2727 Size: 2565 162 KasI G'GCGC_C Cuts at: 0 1542 1864 2081 2727 Size: 1542 322 217 646 Fragments arranged by size: 1542 646 322 217 KpnI G_GTAC'C Cuts at: 0 1176 2727 Size: 1176 1551 MscI TGG'CCA Cuts at: 0 1143 2636 2714 2727 Size: 1143 1493 78 13 Fragments arranged by size: 1493 1143 78 13 MslI CAynn'nnrTG Cuts at: 0 217 349 370 1537 2281 2342 2522 2727 Size: 217 132 21 1167 744 61 180 205 Fragments arranged by size: 1167 744 217 205 180 132 61 21 MspA1I CmG'CkG Cuts at: 0 476 915 1520 2547 2727 Size: 476 439 605 1027 180 Fragments arranged by size: 1027 605 476 439 180 NaeI GCC'GGC Cuts at: 0 333 1911 2727 Size: 333 1578 816 Fragments arranged by size: 1578 816 333 NarI GG'CG_CC Cuts at: 0 1543 1865 2082 2727 Size: 1543 322 217 645 Fragments arranged by size: 1543 645 322 217 NcoI C'CATG_G Cuts at: 0 2709 2727 Size: 2709 18 NdeI CA'TA_TG Cuts at: 0 2114 2727 Size: 2114 613 NgoMIV G'CCGG_C Cuts at: 0 331 1909 2727 Size: 331 1578 818 Fragments arranged by size: 1578 818 331 NspI r_CATG'y Cuts at: 0 375 1259 2182 2286 2727 Size: 375 884 923 104 441 Fragments arranged by size: 923 884 441 375 104 PasI CC'CwG_GG Cuts at: 0 595 2621 2727 Size: 595 2026 106 Fragments arranged by size: 2026 595 106 PflMI CCAn_nnn'nTGG Cuts at: 0 449 941 2727 Size: 449 492 1786 Fragments arranged by size: 1786 492 449 PfoI T'CCnGG_A Cuts at: 0 163 2727 Size: 163 2564 PmlI CAC'GTG Cuts at: 0 1713 2727 Size: 1713 1014 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 106 138 2727 Size: 106 32 2589 Fragments arranged by size: 2589 106 32 PpuMI rG'GwC_Cy Cuts at: 0 240 2727 Size: 240 2487 PspOMI G'GGCC_C Cuts at: 0 1312 2624 2727 Size: 1312 1312 103 Fragments arranged by size: 1312 1312 103 PspXI vC'TCGA_Gb Cuts at: 0 1166 2727 Size: 1166 1561 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1402 1434 2727 Size: 1402 32 1293 Fragments arranged by size: 1402 1293 32 PstI C_TGCA'G Cuts at: 0 587 809 1070 2588 2727 Size: 587 222 261 1518 139 Fragments arranged by size: 1518 587 261 222 139 PvuII CAG'CTG Cuts at: 0 476 2547 2727 Size: 476 2071 180 Fragments arranged by size: 2071 476 180 SacI G_AGCT'C Cuts at: 0 884 2062 2727 Size: 884 1178 665 Fragments arranged by size: 1178 884 665 SacII CC_GC'GG Cuts at: 0 916 2727 Size: 916 1811 SapI GCTCTTCn'nnn_ Cuts at: 0 964 2727 Size: 964 1763 SbfI CC_TGCA'GG Cuts at: 0 2588 2727 Size: 2588 139 SexAI A'CCwGG_T Cuts at: 0 1430 2234 2727 Size: 1430 804 493 Fragments arranged by size: 1430 804 493 SfcI C'TryA_G Cuts at: 0 471 583 805 1050 1066 2584 2727 Size: 471 112 222 245 16 1518 143 Fragments arranged by size: 1518 471 245 222 143 112 16 SfiI GGCCn_nnn'nGGCC Cuts at: 0 2155 2727 Size: 2155 572 SfoI GGC'GCC Cuts at: 0 1544 1866 2083 2727 Size: 1544 322 217 644 Fragments arranged by size: 1544 644 322 217 SmaI CCC'GGG Cuts at: 0 649 2727 Size: 649 2078 SmlI C'TyrA_G Cuts at: 0 886 1166 2329 2727 Size: 886 280 1163 398 Fragments arranged by size: 1163 886 398 280 SphI G_CATG'C Cuts at: 0 2182 2286 2727 Size: 2182 104 441 Fragments arranged by size: 2182 441 104 StuI AGG'CCT Cuts at: 0 2218 2612 2727 Size: 2218 394 115 Fragments arranged by size: 2218 394 115 StyI C'CwwG_G Cuts at: 0 566 2709 2727 Size: 566 2143 18 Fragments arranged by size: 2143 566 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1123 2727 Size: 1123 1604 TatI w'GTAC_w Cuts at: 0 1574 2727 Size: 1574 1153 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2356 2388 2727 Size: 2356 32 339 Fragments arranged by size: 2356 339 32 Tth111I GACn'n_nGTC Cuts at: 0 1288 1806 2727 Size: 1288 518 921 Fragments arranged by size: 1288 921 518 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1926 2727 Size: 1926 801 XhoI C'TCGA_G Cuts at: 0 886 1166 2727 Size: 886 280 1561 Fragments arranged by size: 1561 886 280 XmaI C'CCGG_G Cuts at: 0 647 2727 Size: 647 2080 XmnI GAAnn'nnTTC Cuts at: 0 2347 2727 Size: 2347 380 Enzymes that do cut: AccI Acc65I AcuI AflIII AlfI AloI AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BssHII BssSI BstXI BstYI BstZ17I BtgI BtgZI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PspXI PsrI PstI PvuII SacI SacII SapI SbfI SexAI SfcI SfiI SfoI SmaI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AclI AfeI AflII AgeI AhdI AleI ApoI AscI AseI AsiSI AvrII BaeI BamHI BciVI BfrBI BmtI BplI BsaI BsiWI BsmI BspEI BsrDI BsrGI BstAPI BstBI BstEII Bsu36I BtsI ClaI CspCI DraI DrdI EcoRI FalI FseI FspAI MfeI MluI MmeI NheI NotI NruI NsiI PacI PciI PmeI PshAI PsiI PvuI RsrII SalI SanDI ScaI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XbaI ZraI