(Circular) (Six-base) MAPSORT of: pFN21AB7324.seq Check: 2125 from: 1 to: 8542 pFN21AB7324 8542 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4486 5370 5406 4486 Size: 884 36 7622 Fragments arranged by size: 7622 884 36 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6352 278 Size: 53 83 186 936 4816 2468 Fragments arranged by size: 4816 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5885 1937 Size: 3948 4594 Acc65I G'GTAC_C Cuts at: 5863 5863 Size: 8542 AclI AA'CG_TT Cuts at: 1563 6669 7042 1563 Size: 5106 373 3063 Fragments arranged by size: 5106 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2254 3133 3958 4193 5005 5396 6605 7989 Size: 622 879 825 235 812 391 1209 1384 Cuts at: 7989 8257 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1209 879 825 812 622 391 268 235 AfeI AGC'GCT Cuts at: 3633 5676 3633 Size: 2043 6499 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8345 AflIII A'CryG_T Cuts at: 1203 4230 5097 7441 8418 1203 Size: 3027 867 2344 977 1327 Fragments arranged by size: 3027 2344 1327 977 867 AgeI A'CCGG_T Cuts at: 7373 7373 Size: 8542 AhdI GACnn_n'nnGTC Cuts at: 7271 7271 Size: 8542 AleI CACnn'nnGTG Cuts at: 1166 2324 4733 4754 1166 Size: 1158 2409 21 4954 Fragments arranged by size: 4954 2409 1158 21 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4448 4772 1184 Size: 3264 324 4954 Fragments arranged by size: 4954 3264 324 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2354 2386 3549 3581 3770 3802 3831 3863 3892 Size: 32 1163 32 189 32 29 32 29 Cuts at: 3892 3924 2354 Size: 32 6972 Fragments arranged by size: 6972 1163 189 32 32 32 32 32 29 29 AlwNI CAG_nnn'CTG Cuts at: 3070 3372 3473 4171 4558 5719 6285 7857 3070 Size: 302 101 698 387 1161 566 1572 3755 Fragments arranged by size: 3755 1572 1161 698 566 387 302 101 ApaI G_GGCC'C Cuts at: 2144 2718 3401 4142 5243 5685 2144 Size: 574 683 741 1101 442 5001 Fragments arranged by size: 5001 1101 741 683 574 442 ApaLI G'TGCA_C Cuts at: 2094 4234 4286 5357 6599 7755 2094 Size: 2140 52 1071 1242 1156 2881 Fragments arranged by size: 2881 2140 1242 1156 1071 52 ApoI r'AATT_y Cuts at: 1493 4336 5851 6077 6229 8338 1493 Size: 2843 1515 226 152 2109 1697 Fragments arranged by size: 2843 2109 1697 1515 226 152 AseI AT'TA_AT Cuts at: 160 1032 7096 160 Size: 872 6064 1606 Fragments arranged by size: 6064 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8542 AvaI C'yCGr_G Cuts at: 1870 1943 2163 2322 2685 2845 3042 3655 4135 Size: 73 220 159 363 160 197 613 480 Cuts at: 4135 4805 4832 5462 5612 5678 5867 1870 Size: 670 27 630 150 66 189 4545 Fragments arranged by size: 4545 670 630 613 480 363 220 197 189 160 159 150 73 66 27 AvrII C'CTAG_G Cuts at: 2479 2479 Size: 8542 BamHI G'GATC_C Cuts at: 4848 5872 6239 4848 Size: 1024 367 7151 Fragments arranged by size: 7151 1024 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2579 2654 2801 3046 3354 Size: 325 217 627 792 75 147 245 308 Cuts at: 3354 4691 4748 4767 4859 4886 5036 5375 5406 Size: 1337 57 19 92 27 150 339 31 Cuts at: 5406 5445 5786 5863 7318 618 Size: 39 341 77 1455 1842 Fragments arranged by size: 1842 1455 1337 792 627 341 339 325 308 245 217 150 147 92 77 75 57 39 31 27 19 BanII G_rGCy'C Cuts at: 1391 2144 2253 2412 2684 2718 2846 2933 3011 Size: 753 109 159 272 34 128 87 78 Cuts at: 3011 3401 3768 4134 4142 4683 5243 5463 5633 Size: 390 367 366 8 541 560 220 170 Cuts at: 5633 5685 5861 1391 Size: 52 176 4072 Fragments arranged by size: 4072 753 560 541 390 367 366 272 220 176 170 159 128 109 87 78 52 34 8 BbeI G_GCGC'C Cuts at: 4695 4771 4890 5040 5379 4695 Size: 76 119 150 339 7858 Fragments arranged by size: 7858 339 150 119 76 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2260 4181 5011 928 Size: 971 361 1921 830 4459 Fragments arranged by size: 4459 1921 971 830 361 BbvCI CC'TCA_GC Cuts at: 3791 3852 3913 3791 Size: 61 61 8420 Fragments arranged by size: 8420 61 61 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 6733 6767 1903 Size: 34 50 34 4712 34 3678 Fragments arranged by size: 4712 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3572 6434 7644 3572 Size: 2862 1210 4470 Fragments arranged by size: 4470 2862 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8542 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2109 2688 2780 Size: 107 122 71 631 605 437 579 92 Cuts at: 2780 3386 3405 4317 4970 4979 5034 5165 5415 Size: 606 19 912 653 9 55 131 250 Cuts at: 5415 5496 7153 136 Size: 81 1657 1525 Fragments arranged by size: 1657 1525 912 653 631 606 605 579 437 250 131 122 107 92 81 71 55 19 9 BglII A'GATC_T Cuts at: 8537 8537 Size: 8542 BlpI GC'TnA_GC Cuts at: 3422 5959 3422 Size: 2537 6005 Bme1580I G_kGCm'C Cuts at: 1790 2098 2144 2659 2718 3049 3401 4142 4238 Size: 308 46 515 59 331 352 741 96 Cuts at: 4238 4290 4751 5243 5361 5388 5685 6603 7759 Size: 52 461 492 118 27 297 918 1156 Cuts at: 7759 1790 Size: 2573 Fragments arranged by size: 2573 1156 918 741 515 492 461 352 331 308 297 118 96 59 52 46 27 BmgBI CAC'GTC Cuts at: 1318 5098 1318 Size: 3780 4762 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5216 6273 7231 Size: 416 53 83 399 24 3799 1057 958 Cuts at: 7231 442 Size: 1753 Fragments arranged by size: 3799 1753 1057 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8542 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2313 2313 Size: 8542 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2666 2716 2762 2812 2956 3019 7202 1239 Size: 1427 50 46 50 144 63 4183 2579 Fragments arranged by size: 4183 2579 1427 144 63 50 50 46 Bpu10I CC'TnA_GC Cuts at: 3791 3852 3913 4562 3791 Size: 61 61 649 7771 Fragments arranged by size: 7771 649 61 61 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6667 7532 7830 6667 Size: 865 298 7379 Fragments arranged by size: 7379 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3239 7205 882 Size: 617 1740 3966 2219 Fragments arranged by size: 3966 2219 1740 617 BsaAI yAC'GTr Cuts at: 493 1204 3601 4756 4968 5356 6333 493 Size: 711 2397 1155 212 388 977 2702 Fragments arranged by size: 2702 2397 1155 977 711 388 212 BsaBI GATnn'nnATC Cuts at: 8536 8536 Size: 8542 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2455 2788 4692 4768 Size: 53 83 186 936 922 333 1904 76 Cuts at: 4768 4887 5037 5139 5157 5376 6349 6731 7366 Size: 119 150 102 18 219 973 382 635 Cuts at: 7366 275 Size: 1451 Fragments arranged by size: 1904 1451 973 936 922 635 382 333 219 186 150 119 102 83 76 53 18 BsaWI w'CCGG_w Cuts at: 6975 7373 7647 7794 8205 6975 Size: 398 274 147 411 7312 Fragments arranged by size: 7312 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3454 3484 3549 3579 5450 Size: 30 165 30 2051 30 65 30 1871 Cuts at: 5450 5480 1178 Size: 30 4240 Fragments arranged by size: 4240 2051 1871 165 65 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2420 2909 3221 3449 4823 5384 5471 1186 Size: 1234 489 312 228 1374 561 87 4257 Fragments arranged by size: 4257 1374 1234 561 489 312 228 87 BseYI C'CCAG_C Cuts at: 2150 2411 2591 2650 2667 2737 3225 3670 4682 Size: 261 180 59 17 70 488 445 1012 Cuts at: 4682 4705 5173 5449 5531 5607 5735 7745 8241 Size: 23 468 276 82 76 128 2010 496 Cuts at: 8241 2150 Size: 2451 Fragments arranged by size: 2451 2010 1012 496 488 468 445 276 261 180 128 82 76 70 59 23 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2442 2737 3916 4199 4418 4778 5177 Size: 573 706 295 1179 283 219 360 399 Cuts at: 5177 5384 5560 1163 Size: 207 176 4145 Fragments arranged by size: 4145 1179 706 573 399 360 295 283 219 207 176 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2241 2419 2724 4242 4843 5992 Size: 275 186 240 178 305 1518 601 1149 Cuts at: 5992 6753 7781 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1518 1149 1028 761 601 305 275 240 186 178 BsiHKAI G_wGCw'C Cuts at: 2098 3077 4042 4134 4185 4238 4290 4911 5070 Size: 979 965 92 51 53 52 621 159 Cuts at: 5070 5361 6603 6688 7759 2098 Size: 291 1242 85 1071 2881 Fragments arranged by size: 2881 1242 1071 979 965 621 291 159 92 85 53 52 51 BsmI GAATG_Cn' Cuts at: 3986 6053 6146 3986 Size: 2067 93 6382 Fragments arranged by size: 6382 2067 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2320 2320 Size: 8542 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2098 2144 2253 2412 2659 2684 2718 Size: 399 308 46 109 159 247 25 34 Cuts at: 2718 2846 2933 3011 3049 3077 3401 3768 4042 Size: 128 87 78 38 28 324 367 274 Cuts at: 4042 4134 4142 4185 4238 4290 4683 4751 4911 Size: 92 8 43 53 52 393 68 160 Cuts at: 4911 5070 5243 5361 5388 5463 5633 5685 5861 Size: 159 173 118 27 75 170 52 176 Cuts at: 5861 6603 6688 7759 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 393 367 324 308 274 247 176 173 170 160 159 159 128 118 109 92 87 85 78 75 68 53 52 52 46 43 38 34 28 27 25 8 BspEI T'CCGG_A Cuts at: 8205 8205 Size: 8542 BspHI T'CATG_A Cuts at: 6431 8161 6431 Size: 1730 6812 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2403 4486 4773 5268 5370 5406 5897 844 Size: 1559 2083 287 495 102 36 491 3489 Fragments arranged by size: 3489 2083 1559 495 491 287 102 36 BsrBI CCG'CTC Cuts at: 1394 1823 6429 1394 Size: 429 4606 3507 Fragments arranged by size: 4606 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 7037 7211 65 Size: 1183 5789 174 1396 Fragments arranged by size: 5789 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2181 4320 4376 5296 5442 5798 7186 Size: 110 371 2139 56 920 146 356 1388 Cuts at: 7186 7373 1700 Size: 187 2869 Fragments arranged by size: 2869 2139 1388 920 371 356 187 146 110 56 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8542 BssHII G'CGCG_C Cuts at: 1925 4930 1925 Size: 3005 5537 BssSI C'ACGA_G Cuts at: 2677 3092 6602 7614 2677 Size: 415 3510 1012 3605 Fragments arranged by size: 3605 3510 1012 415 BstAPI GCAn_nnn'nTGC Cuts at: 794 3473 4558 4877 794 Size: 2679 1085 319 4459 Fragments arranged by size: 4459 2679 1085 319 BstBI TT'CG_AA Cuts at: 7346 7346 Size: 8542 BstEII G'GTnAC_C Cuts at: 1184 3749 4498 4726 5602 1184 Size: 2565 749 228 876 4124 Fragments arranged by size: 4124 2565 876 749 228 BstXI CCAn_nnnn'nTGG Cuts at: 2326 2836 3257 3707 5539 2326 Size: 510 421 450 1832 5329 Fragments arranged by size: 5329 1832 510 450 421 BstYI r'GATC_y Cuts at: 1972 4168 4848 5872 6239 6624 6641 8093 8179 Size: 2196 680 1024 367 385 17 1452 86 Cuts at: 8179 8191 8537 1972 Size: 12 346 1977 Fragments arranged by size: 2196 1977 1452 1024 680 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3432 4114 3432 Size: 682 7860 BtgI C'CryG_G Cuts at: 513 1065 2014 2718 2757 3250 4051 4221 4843 Size: 552 949 704 39 493 801 170 622 Cuts at: 4843 5216 5598 513 Size: 373 382 3457 Fragments arranged by size: 3457 949 801 704 622 552 493 382 373 170 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7803 Fragments arranged by size: 7803 683 56 BtsI GCAGTG_nn' Cuts at: 2098 4023 5077 5111 5323 5626 6058 6852 6872 Size: 1925 1054 34 212 303 432 794 20 Cuts at: 6872 2098 Size: 3768 Fragments arranged by size: 3768 1925 1054 794 432 303 212 34 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8542 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4089 4124 5267 5302 529 Size: 35 3525 35 1143 35 3769 Fragments arranged by size: 3769 3525 1143 35 35 35 DraI TTT'AAA Cuts at: 5846 5846 Size: 8542 DraIII CAC_nnn'GTG Cuts at: 4222 5307 4222 Size: 1085 7457 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2122 7549 809 Size: 507 806 5427 1802 Fragments arranged by size: 5427 1802 806 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2238 2416 2721 Size: 54 1425 50 275 18 408 178 305 Cuts at: 2721 3347 3378 3490 3697 4239 4840 4853 4962 Size: 626 31 112 207 542 601 13 109 Cuts at: 4962 5298 5686 5989 6878 8507 8 Size: 336 388 303 889 1629 43 Fragments arranged by size: 1629 1425 889 626 601 542 408 388 336 305 303 275 207 178 112 109 54 50 43 31 18 13 EagI C'GGCC_G Cuts at: 1537 1812 2238 2416 2721 4239 4840 5989 1537 Size: 275 426 178 305 1518 601 1149 4090 Fragments arranged by size: 4090 1518 1149 601 426 305 275 178 EarI CTCTTCn'nnn_ Cuts at: 1353 2304 3527 3996 5066 6472 1353 Size: 951 1223 469 1070 1406 3423 Fragments arranged by size: 3423 1406 1223 1070 951 469 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3557 5357 7129 7503 7649 387 Size: 1064 138 1968 1800 1772 374 146 1280 Fragments arranged by size: 1968 1800 1772 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4132 4132 Size: 8542 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2254 2666 2716 2762 2812 2956 3019 Size: 393 622 412 50 46 50 144 63 Cuts at: 3019 3133 3958 4193 5005 5396 6605 7202 7989 Size: 114 825 235 812 391 1209 597 787 Cuts at: 7989 8257 1239 Size: 268 1524 Fragments arranged by size: 1524 1209 825 812 787 622 597 412 393 391 268 235 144 114 63 50 50 46 EcoNI CCTnn'n_nnAGG Cuts at: 2986 3349 3431 4412 5366 2986 Size: 363 82 981 954 6162 Fragments arranged by size: 6162 981 954 363 82 EcoO109I rG'GnC_Cy Cuts at: 2140 2194 2378 2991 3322 3369 3481 3652 3661 Size: 54 184 613 331 47 112 171 9 Cuts at: 3661 4078 4510 4588 5240 5816 2140 Size: 417 432 78 652 576 4866 Fragments arranged by size: 4866 652 613 576 432 417 331 184 171 112 78 54 47 9 EcoRI G'AATT_C Cuts at: 5851 6229 5851 Size: 378 8164 FspI TGC'GCA Cuts at: 4796 7048 4796 Size: 2252 6290 HaeII r_GCGC'y Cuts at: 1678 3031 3610 3635 4299 4695 4771 4890 4985 Size: 1353 579 25 664 396 76 119 95 Cuts at: 4985 5040 5379 5678 7689 1678 Size: 55 339 299 2011 2531 Fragments arranged by size: 2531 2011 1353 664 579 396 339 299 119 95 76 55 25 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3200 3232 4153 4185 7183 Size: 32 135 32 1628 32 921 32 2998 Cuts at: 7183 7215 7257 7289 1373 Size: 32 42 32 2626 Fragments arranged by size: 2998 2626 1628 921 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5886 6132 669 Size: 988 281 3948 246 3079 Fragments arranged by size: 3948 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8542 HpaI GTT'AAC Cuts at: 6132 6132 Size: 8542 KasI G'GCGC_C Cuts at: 4691 4767 4886 5036 5375 4691 Size: 76 119 150 339 7858 Fragments arranged by size: 7858 339 150 119 76 KpnI G_GTAC'C Cuts at: 5867 5867 Size: 8542 MfeI C'AATT_G Cuts at: 6141 6141 Size: 8542 MluI A'CGCG_T Cuts at: 4230 8418 4230 Size: 4188 4354 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3780 3841 3902 7656 7840 1305 Size: 2475 61 61 3754 184 2007 Fragments arranged by size: 3754 2475 2007 184 61 61 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6720 Fragments arranged by size: 6720 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2260 2324 3705 4733 4754 6500 6859 Size: 648 1094 64 1381 1028 21 1746 359 Cuts at: 6859 7018 518 Size: 159 2042 Fragments arranged by size: 2042 1746 1381 1094 1028 648 359 159 64 21 MspA1I CmG'CkG Cuts at: 1606 2150 2415 2438 3229 3414 3670 3685 3982 Size: 544 265 23 791 185 256 15 297 Cuts at: 3982 4144 4581 4762 4816 4927 5025 5646 5958 Size: 162 437 181 54 111 98 621 312 Cuts at: 5958 6635 7783 8028 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 791 677 621 544 437 312 297 265 256 245 185 181 162 111 98 54 23 15 NaeI GCC'GGC Cuts at: 4322 4378 5444 4322 Size: 56 1066 7420 Fragments arranged by size: 7420 1066 56 NarI GG'CG_CC Cuts at: 4692 4768 4887 5037 5376 4692 Size: 76 119 150 339 7858 Fragments arranged by size: 7858 339 150 119 76 NcoI C'CATG_G Cuts at: 513 1065 2757 5598 513 Size: 552 1692 2841 3457 Fragments arranged by size: 3457 2841 1692 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8542 NgoMIV G'CCGG_C Cuts at: 4320 4376 5442 4320 Size: 56 1066 7420 Fragments arranged by size: 7420 1066 56 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8542 NotI GC'GGCC_GC Cuts at: 5989 5989 Size: 8542 NruI TCG'CGA Cuts at: 3168 3168 Size: 8542 NspI r_CATG'y Cuts at: 1132 1229 5121 5900 7445 1132 Size: 97 3892 779 1545 2229 Fragments arranged by size: 3892 2229 1545 779 97 PasI CC'CwG_GG Cuts at: 1348 2659 2793 4202 4775 5053 5581 1348 Size: 1311 134 1409 573 278 528 4309 Fragments arranged by size: 4309 1409 1311 573 528 278 134 PciI A'CATG_T Cuts at: 7441 7441 Size: 8542 PflMI CCAn_nnn'nTGG Cuts at: 1108 2150 4420 4461 5008 1108 Size: 1042 2270 41 547 4642 Fragments arranged by size: 4642 2270 1042 547 41 PfoI T'CCnGG_A Cuts at: 2215 2611 2933 5007 2215 Size: 396 322 2074 5750 Fragments arranged by size: 5750 2074 396 322 PmeI GTTT'AAAC Cuts at: 5846 5846 Size: 8542 PmlI CAC'GTG Cuts at: 4968 5356 4968 Size: 388 8154 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3549 3581 4895 4927 6611 6643 8124 8156 3549 Size: 32 1314 32 1684 32 1481 32 3935 Fragments arranged by size: 3935 1684 1481 1314 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2378 2991 3481 3652 3661 4588 5816 2378 Size: 613 490 171 9 927 1228 5104 Fragments arranged by size: 5104 1228 927 613 490 171 9 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8542 PsiI TTA'TAA Cuts at: 6112 6112 Size: 8542 PspOMI G'GGCC_C Cuts at: 2140 2714 3397 4138 5239 5681 2140 Size: 574 683 741 1101 442 5001 Fragments arranged by size: 5001 1101 741 683 574 442 PstI C_TGCA'G Cuts at: 830 3413 3669 3810 3871 3932 4033 4902 5265 Size: 2583 256 141 61 61 101 869 363 Cuts at: 5265 5367 5894 830 Size: 102 527 3478 Fragments arranged by size: 3478 2583 869 527 363 256 141 102 101 61 61 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8542 PvuII CAG'CTG Cuts at: 2438 3670 3982 4762 4927 5025 5646 2438 Size: 1232 312 780 165 98 621 5334 Fragments arranged by size: 5334 1232 780 621 312 165 98 SacI G_AGCT'C Cuts at: 4134 4134 Size: 8542 SalI G'TCGA_C Cuts at: 1936 5884 1936 Size: 3948 4594 SanDI GG'GwC_CC Cuts at: 3481 3652 4588 5816 3481 Size: 171 936 1228 6207 Fragments arranged by size: 6207 1228 936 171 SapI GCTCTTCn'nnn_ Cuts at: 2304 3527 2304 Size: 1223 7319 SbfI CC_TGCA'GG Cuts at: 5265 5367 5894 5265 Size: 102 527 7913 Fragments arranged by size: 7913 527 102 ScaI AGT'ACT Cuts at: 6790 8502 6790 Size: 1712 6830 SfcI C'TryA_G Cuts at: 826 1045 3409 3665 3806 3867 3928 4029 4898 Size: 219 2364 256 141 61 61 101 869 Cuts at: 4898 5261 5363 5641 5890 7025 7706 7897 826 Size: 363 102 278 249 1135 681 191 1471 Fragments arranged by size: 2364 1471 1135 869 681 363 278 256 249 219 191 141 102 101 61 61 SfiI GGCCn_nnn'nGGCC Cuts at: 2780 3386 4970 2780 Size: 606 1584 6352 Fragments arranged by size: 6352 1584 606 SfoI GGC'GCC Cuts at: 4693 4769 4888 5038 5377 4693 Size: 76 119 150 339 7858 Fragments arranged by size: 7858 339 150 119 76 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8542 SgrAI Cr'CCGG_yG Cuts at: 1700 4376 1700 Size: 2676 5866 SmaI CCC'GGG Cuts at: 1872 2165 2687 3044 3657 5869 1872 Size: 293 522 357 613 2212 4545 Fragments arranged by size: 4545 2212 613 522 357 293 SmlI C'TyrA_G Cuts at: 820 1017 1943 6646 7547 7809 820 Size: 197 926 4703 901 262 1553 Fragments arranged by size: 4703 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6333 493 Size: 5840 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8542 SphI G_CATG'C Cuts at: 1132 5121 5900 1132 Size: 3989 779 3774 Fragments arranged by size: 3989 3774 779 SspI AAT'ATT Cuts at: 5 52 6466 5 Size: 47 6414 2081 Fragments arranged by size: 6414 2081 47 StuI AGG'CCT Cuts at: 1517 3186 3239 3344 3389 5775 1517 Size: 1669 53 105 45 2386 4284 Fragments arranged by size: 4284 2386 1669 105 53 45 StyI C'CwwG_G Cuts at: 513 1065 2479 2757 3159 3201 3256 3381 5598 Size: 552 1414 278 402 42 55 125 2217 Cuts at: 5598 5754 5981 513 Size: 156 227 3074 Fragments arranged by size: 3074 2217 1414 552 402 278 227 156 125 55 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2586 2642 2963 4788 5247 5463 5594 Size: 1255 1006 56 321 1825 459 216 131 Cuts at: 5594 5621 6569 6586 6739 6924 7369 325 Size: 27 948 17 153 185 445 1498 Fragments arranged by size: 1825 1498 1255 1006 948 459 445 321 216 185 153 131 56 27 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3266 4737 6788 Size: 274 80 33 51 1443 1289 1471 2051 Cuts at: 6788 8500 96 Size: 1712 138 Fragments arranged by size: 2051 1712 1471 1443 1289 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3717 3749 4032 4064 4090 4122 5049 5081 5468 Size: 32 283 32 26 32 927 32 387 Cuts at: 5468 5500 8502 8534 3717 Size: 32 3002 32 3725 Fragments arranged by size: 3725 3002 927 387 283 32 32 32 32 32 32 26 Tth111I GACn'n_nGTC Cuts at: 1316 5015 5713 1316 Size: 3699 698 4145 Fragments arranged by size: 4145 3699 698 XbaI T'CTAG_A Cuts at: 5878 5878 Size: 8542 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4720 4720 Size: 8542 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8542 XmaI C'CCGG_G Cuts at: 1870 2163 2685 3042 3655 5867 1870 Size: 293 522 357 613 2212 4545 Fragments arranged by size: 4545 2212 613 522 357 293 XmnI GAAnn'nnTTC Cuts at: 6671 6671 Size: 8542 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6350 276 Size: 53 83 186 936 4816 2468 Fragments arranged by size: 4816 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BfrBI BsiWI BstZ17I EcoRV FalI FseI FspAI NsiI PacI PspXI PsrI RsrII SacII SexAI SrfI SwaI