Restriction map for
pFN21AB7324
(Linear) (Six-base) MAPSORT of: KIBB7324.seq Check: 494 from: 1 to: 3837
KIBB7324.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2481 3365 3401 3837
Size: 2481 884 36 436
Fragments arranged by size:
2481 884 436 36
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 249 1128 1953 2188 3000 3391 3837
Size: 249 879 825 235 812 391 446
Fragments arranged by size:
879 825 812 446 391 249 235
AfeI AGC'GCT
Cuts at: 0 1628 3671 3837
Size: 1628 2043 166
Fragments arranged by size:
2043 1628 166
AflIII A'CryG_T
Cuts at: 0 2225 3092 3837
Size: 2225 867 745
Fragments arranged by size:
2225 867 745
AleI CACnn'nnGTG
Cuts at: 0 319 2728 2749 3837
Size: 319 2409 21 1088
Fragments arranged by size:
2409 1088 319 21
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2443 2767 3837
Size: 2443 324 1070
Fragments arranged by size:
2443 1070 324
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 349 381 1544 1576 1765 1797 1826 1858
Size: 349 32 1163 32 189 32 29 32
Cuts at: 1858 1887 1919 3837
Size: 29 32 1918
Fragments arranged by size:
1918 1163 349 189 32 32 32 32
32 29 29
AlwNI CAG_nnn'CTG
Cuts at: 0 1065 1367 1468 2166 2553 3714 3837
Size: 1065 302 101 698 387 1161 123
Fragments arranged by size:
1161 1065 698 387 302 123 101
ApaI G_GGCC'C
Cuts at: 0 139 713 1396 2137 3238 3680 3837
Size: 139 574 683 741 1101 442 157
Fragments arranged by size:
1101 741 683 574 442 157 139
ApaLI G'TGCA_C
Cuts at: 0 89 2229 2281 3352 3837
Size: 89 2140 52 1071 485
Fragments arranged by size:
2140 1071 485 89 52
ApoI r'AATT_y
Cuts at: 0 2331 3837
Size: 2331 1506
AvaI C'yCGr_G
Cuts at: 0 158 317 680 840 1037 1650 2130 2800
Size: 158 159 363 160 197 613 480 670
Cuts at: 2800 2827 3457 3607 3673 3837
Size: 27 630 150 66 164
Fragments arranged by size:
670 630 613 480 363 197 164 160
159 158 150 66 27
AvrII C'CTAG_G
Cuts at: 0 474 3837
Size: 474 3363
BamHI G'GATC_C
Cuts at: 0 2843 3837
Size: 2843 994
BanI G'GyrC_C
Cuts at: 0 574 649 796 1041 1349 2686 2743 2762
Size: 574 75 147 245 308 1337 57 19
Cuts at: 2762 2854 2881 3031 3370 3401 3440 3781 3837
Size: 92 27 150 339 31 39 341 56
Fragments arranged by size:
1337 574 341 339 308 245 150 147
92 75 57 56 39 31 27 19
BanII G_rGCy'C
Cuts at: 0 139 248 407 679 713 841 928 1006
Size: 139 109 159 272 34 128 87 78
Cuts at: 1006 1396 1763 2129 2137 2678 3238 3458 3628
Size: 390 367 366 8 541 560 220 170
Cuts at: 3628 3680 3837
Size: 52 157
Fragments arranged by size:
560 541 390 367 366 272 220 170
159 157 139 128 109 87 78 52
34 8
BbeI G_GCGC'C
Cuts at: 0 2690 2766 2885 3035 3374 3837
Size: 2690 76 119 150 339 463
Fragments arranged by size:
2690 463 339 150 119 76
BbsI GAAGACnn'nnnn_
Cuts at: 0 255 2176 3006 3837
Size: 255 1921 830 831
Fragments arranged by size:
1921 831 830 255
BbvCI CC'TCA_GC
Cuts at: 0 1786 1847 1908 3837
Size: 1786 61 61 1929
Fragments arranged by size:
1929 1786 61 61
BciVI GTATCCnnnnn_n'
Cuts at: 0 1567 3837
Size: 1567 2270
BglI GCCn_nnn'nGGC
Cuts at: 0 104 683 775 1381 1400 2312 2965 2974
Size: 104 579 92 606 19 912 653 9
Cuts at: 2974 3029 3160 3410 3491 3837
Size: 55 131 250 81 346
Fragments arranged by size:
912 653 606 579 346 250 131 104
92 81 55 19 9
BlpI GC'TnA_GC
Cuts at: 0 1417 3837
Size: 1417 2420
Bme1580I G_kGCm'C
Cuts at: 0 93 139 654 713 1044 1396 2137 2233
Size: 93 46 515 59 331 352 741 96
Cuts at: 2233 2285 2746 3238 3356 3383 3680 3837
Size: 52 461 492 118 27 297 157
Fragments arranged by size:
741 515 492 461 352 331 297 157
118 96 93 59 52 46 27
BmgBI CAC'GTC
Cuts at: 0 3093 3837
Size: 3093 744
BmrI ACTGGGnnnn_n'
Cuts at: 0 3211 3837
Size: 3211 626
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 308 3837
Size: 308 3529
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 661 711 757 807 951 1014 3837
Size: 661 50 46 50 144 63 2823
Fragments arranged by size:
2823 661 144 63 50 50 46
Bpu10I CC'TnA_GC
Cuts at: 0 1786 1847 1908 2557 3837
Size: 1786 61 61 649 1280
Fragments arranged by size:
1786 1280 649 61 61
BsaI GGTCTCn'nnnn_
Cuts at: 0 1234 3837
Size: 1234 2603
BsaAI yAC'GTr
Cuts at: 0 1596 2751 2963 3351 3837
Size: 1596 1155 212 388 486
Fragments arranged by size:
1596 1155 486 388 212
BsaHI Gr'CG_yC
Cuts at: 0 450 783 2687 2763 2882 3032 3134 3152
Size: 450 333 1904 76 119 150 102 18
Cuts at: 3152 3371 3837
Size: 219 466
Fragments arranged by size:
1904 466 450 333 219 150 119 102
76 18
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1449 1479 1544 1574 3445 3475 3837
Size: 1449 30 65 30 1871 30 362
Fragments arranged by size:
1871 1449 362 65 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 415 904 1216 1444 2818 3379 3466 3837
Size: 415 489 312 228 1374 561 87 371
Fragments arranged by size:
1374 561 489 415 371 312 228 87
BseYI C'CCAG_C
Cuts at: 0 145 406 586 645 662 732 1220 1665
Size: 145 261 180 59 17 70 488 445
Cuts at: 1665 2677 2700 3168 3444 3526 3602 3730 3837
Size: 1012 23 468 276 82 76 128 107
Fragments arranged by size:
1012 488 468 445 276 261 180 145
128 107 82 76 70 59 23 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 437 732 1911 2194 2413 2773 3172 3379
Size: 437 295 1179 283 219 360 399 207
Cuts at: 3379 3555 3837
Size: 176 282
Fragments arranged by size:
1179 437 399 360 295 283 282 219
207 176
BsiEI CG_ry'CG
Cuts at: 0 236 414 719 2237 2838 3837
Size: 236 178 305 1518 601 999
Fragments arranged by size:
1518 999 601 305 236 178
BsiHKAI G_wGCw'C
Cuts at: 0 93 1072 2037 2129 2180 2233 2285 2906
Size: 93 979 965 92 51 53 52 621
Cuts at: 2906 3065 3356 3837
Size: 159 291 481
Fragments arranged by size:
979 965 621 481 291 159 93 92
53 52 51
BsmI GAATG_Cn'
Cuts at: 0 1981 3837
Size: 1981 1856
BsmBI CGTCTCn'nnnn_
Cuts at: 0 315 3837
Size: 315 3522
Bsp1286I G_dGCh'C
Cuts at: 0 93 139 248 407 654 679 713 841
Size: 93 46 109 159 247 25 34 128
Cuts at: 841 928 1006 1044 1072 1396 1763 2037 2129
Size: 87 78 38 28 324 367 274 92
Cuts at: 2129 2137 2180 2233 2285 2678 2746 2906 3065
Size: 8 43 53 52 393 68 160 159
Cuts at: 3065 3238 3356 3383 3458 3628 3680 3837
Size: 173 118 27 75 170 52 157
Fragments arranged by size:
393 367 324 274 247 173 170 160
159 159 157 128 118 109 93 92
87 78 75 68 53 52 52 46
43 38 34 28 27 25 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 398 2481 2768 3263 3365 3401 3837
Size: 398 2083 287 495 102 36 436
Fragments arranged by size:
2083 495 436 398 287 102 36
BsrFI r'CCGG_y
Cuts at: 0 176 2315 2371 3291 3437 3793 3837
Size: 176 2139 56 920 146 356 44
Fragments arranged by size:
2139 920 356 176 146 56 44
BssHII G'CGCG_C
Cuts at: 0 2925 3837
Size: 2925 912
BssSI C'ACGA_G
Cuts at: 0 672 1087 3837
Size: 672 415 2750
Fragments arranged by size:
2750 672 415
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1468 2553 2872 3837
Size: 1468 1085 319 965
Fragments arranged by size:
1468 1085 965 319
BstEII G'GTnAC_C
Cuts at: 0 1744 2493 2721 3597 3837
Size: 1744 749 228 876 240
Fragments arranged by size:
1744 876 749 240 228
BstXI CCAn_nnnn'nTGG
Cuts at: 0 321 831 1252 1702 3534 3837
Size: 321 510 421 450 1832 303
Fragments arranged by size:
1832 510 450 421 321 303
BstYI r'GATC_y
Cuts at: 0 2163 2843 3837
Size: 2163 680 994
Fragments arranged by size:
2163 994 680
Bsu36I CC'TnA_GG
Cuts at: 0 1427 2109 3837
Size: 1427 682 1728
Fragments arranged by size:
1728 1427 682
BtgI C'CryG_G
Cuts at: 0 9 713 752 1245 2046 2216 2838 3211
Size: 9 704 39 493 801 170 622 373
Cuts at: 3211 3593 3837
Size: 382 244
Fragments arranged by size:
801 704 622 493 382 373 244 170
39 9
BtsI GCAGTG_nn'
Cuts at: 0 93 2018 3072 3106 3318 3621 3837
Size: 93 1925 1054 34 212 303 216
Fragments arranged by size:
1925 1054 303 216 212 93 34
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2084 2119 3262 3297 3837
Size: 2084 35 1143 35 540
Fragments arranged by size:
2084 1143 540 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 2217 3302 3837
Size: 2217 1085 535
Fragments arranged by size:
2217 1085 535
DrdI GACnn_nn'nnGTC
Cuts at: 0 117 3837
Size: 117 3720
EaeI y'GGCC_r
Cuts at: 0 233 411 716 1342 1373 1485 1692 2234
Size: 233 178 305 626 31 112 207 542
Cuts at: 2234 2835 2848 2957 3293 3681 3837
Size: 601 13 109 336 388 156
Fragments arranged by size:
626 601 542 388 336 305 233 207
178 156 112 109 31 13
EagI C'GGCC_G
Cuts at: 0 233 411 716 2234 2835 3837
Size: 233 178 305 1518 601 1002
Fragments arranged by size:
1518 1002 601 305 233 178
EarI CTCTTCn'nnn_
Cuts at: 0 299 1522 1991 3061 3837
Size: 299 1223 469 1070 776
Fragments arranged by size:
1223 1070 776 469 299
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1552 3352 3837
Size: 1552 1800 485
Fragments arranged by size:
1800 1552 485
EcoICRI GAG'CTC
Cuts at: 0 2127 3837
Size: 2127 1710
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 249 661 711 757 807 951 1014 1128
Size: 249 412 50 46 50 144 63 114
Cuts at: 1128 1953 2188 3000 3391 3837
Size: 825 235 812 391 446
Fragments arranged by size:
825 812 446 412 391 249 235 144
114 63 50 50 46
EcoNI CCTnn'n_nnAGG
Cuts at: 0 981 1344 1426 2407 3361 3837
Size: 981 363 82 981 954 476
Fragments arranged by size:
981 981 954 476 363 82
EcoO109I rG'GnC_Cy
Cuts at: 0 135 189 373 986 1317 1364 1476 1647
Size: 135 54 184 613 331 47 112 171
Cuts at: 1647 1656 2073 2505 2583 3235 3811 3837
Size: 9 417 432 78 652 576 26
Fragments arranged by size:
652 613 576 432 417 331 184 171
135 112 78 54 47 26 9
FspI TGC'GCA
Cuts at: 0 2791 3837
Size: 2791 1046
HaeII r_GCGC'y
Cuts at: 0 1026 1605 1630 2294 2690 2766 2885 2980
Size: 1026 579 25 664 396 76 119 95
Cuts at: 2980 3035 3374 3673 3837
Size: 55 339 299 164
Fragments arranged by size:
1026 664 579 396 339 299 164 119
95 76 55 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1195 1227 2148 2180 3837
Size: 1195 32 921 32 1657
Fragments arranged by size:
1657 1195 921 32 32
KasI G'GCGC_C
Cuts at: 0 2686 2762 2881 3031 3370 3837
Size: 2686 76 119 150 339 467
Fragments arranged by size:
2686 467 339 150 119 76
MluI A'CGCG_T
Cuts at: 0 2225 3837
Size: 2225 1612
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1775 1836 1897 3837
Size: 1775 61 61 1940
Fragments arranged by size:
1940 1775 61 61
MslI CAynn'nnrTG
Cuts at: 0 255 319 1700 2728 2749 3837
Size: 255 64 1381 1028 21 1088
Fragments arranged by size:
1381 1088 1028 255 64 21
MspA1I CmG'CkG
Cuts at: 0 145 410 433 1224 1409 1665 1680 1977
Size: 145 265 23 791 185 256 15 297
Cuts at: 1977 2139 2576 2757 2811 2922 3020 3641 3837
Size: 162 437 181 54 111 98 621 196
Fragments arranged by size:
791 621 437 297 265 256 196 185
181 162 145 111 98 54 23 15
NaeI GCC'GGC
Cuts at: 0 2317 2373 3439 3837
Size: 2317 56 1066 398
Fragments arranged by size:
2317 1066 398 56
NarI GG'CG_CC
Cuts at: 0 2687 2763 2882 3032 3371 3837
Size: 2687 76 119 150 339 466
Fragments arranged by size:
2687 466 339 150 119 76
NcoI C'CATG_G
Cuts at: 0 752 3593 3837
Size: 752 2841 244
Fragments arranged by size:
2841 752 244
NgoMIV G'CCGG_C
Cuts at: 0 2315 2371 3437 3837
Size: 2315 56 1066 400
Fragments arranged by size:
2315 1066 400 56
NruI TCG'CGA
Cuts at: 0 1163 3837
Size: 1163 2674
NspI r_CATG'y
Cuts at: 0 3116 3837
Size: 3116 721
PasI CC'CwG_GG
Cuts at: 0 654 788 2197 2770 3048 3576 3837
Size: 654 134 1409 573 278 528 261
Fragments arranged by size:
1409 654 573 528 278 261 134
PflMI CCAn_nnn'nTGG
Cuts at: 0 145 2415 2456 3003 3837
Size: 145 2270 41 547 834
Fragments arranged by size:
2270 834 547 145 41
PfoI T'CCnGG_A
Cuts at: 0 210 606 928 3002 3837
Size: 210 396 322 2074 835
Fragments arranged by size:
2074 835 396 322 210
PmlI CAC'GTG
Cuts at: 0 2963 3351 3837
Size: 2963 388 486
Fragments arranged by size:
2963 486 388
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1544 1576 2890 2922 3837
Size: 1544 32 1314 32 915
Fragments arranged by size:
1544 1314 915 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 373 986 1476 1647 1656 2583 3811 3837
Size: 373 613 490 171 9 927 1228 26
Fragments arranged by size:
1228 927 613 490 373 171 26 9
PspOMI G'GGCC_C
Cuts at: 0 135 709 1392 2133 3234 3676 3837
Size: 135 574 683 741 1101 442 161
Fragments arranged by size:
1101 741 683 574 442 161 135
PstI C_TGCA'G
Cuts at: 0 1408 1664 1805 1866 1927 2028 2897 3260
Size: 1408 256 141 61 61 101 869 363
Cuts at: 3260 3362 3837
Size: 102 475
Fragments arranged by size:
1408 869 475 363 256 141 102 101
61 61
PvuII CAG'CTG
Cuts at: 0 433 1665 1977 2757 2922 3020 3641 3837
Size: 433 1232 312 780 165 98 621 196
Fragments arranged by size:
1232 780 621 433 312 196 165 98
SacI G_AGCT'C
Cuts at: 0 2129 3837
Size: 2129 1708
SanDI GG'GwC_CC
Cuts at: 0 1476 1647 2583 3811 3837
Size: 1476 171 936 1228 26
Fragments arranged by size:
1476 1228 936 171 26
SapI GCTCTTCn'nnn_
Cuts at: 0 299 1522 3837
Size: 299 1223 2315
Fragments arranged by size:
2315 1223 299
SbfI CC_TGCA'GG
Cuts at: 0 3260 3362 3837
Size: 3260 102 475
Fragments arranged by size:
3260 475 102
SfcI C'TryA_G
Cuts at: 0 1404 1660 1801 1862 1923 2024 2893 3256
Size: 1404 256 141 61 61 101 869 363
Cuts at: 3256 3358 3636 3837
Size: 102 278 201
Fragments arranged by size:
1404 869 363 278 256 201 141 102
101 61 61
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 775 1381 2965 3837
Size: 775 606 1584 872
Fragments arranged by size:
1584 872 775 606
SfoI GGC'GCC
Cuts at: 0 2688 2764 2883 3033 3372 3837
Size: 2688 76 119 150 339 465
Fragments arranged by size:
2688 465 339 150 119 76
SgrAI Cr'CCGG_yG
Cuts at: 0 2371 3837
Size: 2371 1466
SmaI CCC'GGG
Cuts at: 0 160 682 1039 1652 3837
Size: 160 522 357 613 2185
Fragments arranged by size:
2185 613 522 357 160
SphI G_CATG'C
Cuts at: 0 3116 3837
Size: 3116 721
StuI AGG'CCT
Cuts at: 0 1181 1234 1339 1384 3770 3837
Size: 1181 53 105 45 2386 67
Fragments arranged by size:
2386 1181 105 67 53 45
StyI C'CwwG_G
Cuts at: 0 474 752 1154 1196 1251 1376 3593 3749
Size: 474 278 402 42 55 125 2217 156
Cuts at: 3749 3837
Size: 88
Fragments arranged by size:
2217 474 402 278 156 125 88 55
42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 581 637 958 2783 3242 3458 3589 3616
Size: 581 56 321 1825 459 216 131 27
Cuts at: 3616 3837
Size: 221
Fragments arranged by size:
1825 581 459 321 221 216 131 56
27
TatI w'GTAC_w
Cuts at: 0 1261 2732 3837
Size: 1261 1471 1105
Fragments arranged by size:
1471 1261 1105
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1712 1744 2027 2059 2085 2117 3044 3076
Size: 1712 32 283 32 26 32 927 32
Cuts at: 3076 3463 3495 3837
Size: 387 32 342
Fragments arranged by size:
1712 927 387 342 283 32 32 32
32 32 26
Tth111I GACn'n_nGTC
Cuts at: 0 3010 3708 3837
Size: 3010 698 129
Fragments arranged by size:
3010 698 129
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2715 3837
Size: 2715 1122
XmaI C'CCGG_G
Cuts at: 0 158 680 1037 1650 3837
Size: 158 522 357 613 2187
Fragments arranged by size:
2187 613 522 357 158
Enzymes that do cut:
AarI AcuI AfeI AflIII AleI AlfI AloI AlwNI
ApaI ApaLI ApoI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BciVI BglI BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtsI CspCI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I KasI MluI MmeI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NspI PasI PflMI PfoI PmlI
PpiI PpuMI PspOMI PstI PvuII SacI SanDI SapI
SbfI SfcI SfiI SfoI SgrAI SmaI SphI StuI
StyI TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AscI
AseI AsiSI BaeI BcgI BclI BfrBI BglII BmtI
BpuEI BsaBI BsaWI BsiWI BspEI BspHI BsrBI BsrDI
BsrGI BstBI BstZ17I BtgZI ClaI DraI EcoRI EcoRV
FalI FseI FspAI HincII HindIII HpaI KpnI MfeI
MscI NdeI NheI NotI NsiI PacI PciI PmeI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
ScaI SexAI SgfI SmlI SnaBI SpeI SrfI SspI
SwaI XbaI XhoI XmnI ZraI