(Circular) (Six-base) MAPSORT of: pFN21AE0470.seq Check: 2488 from: 1 to: 5608 pFN21AE0470 5608 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3418 278 Size: 53 83 186 936 1882 2468 Fragments arranged by size: 2468 1882 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2951 1937 Size: 1014 4594 Acc65I G'GTAC_C Cuts at: 2085 2929 2085 Size: 844 4764 AclI AA'CG_TT Cuts at: 1563 3735 4108 1563 Size: 2172 373 3063 Fragments arranged by size: 3063 2172 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3671 5055 5323 1632 Size: 2039 1384 268 1917 Fragments arranged by size: 2039 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5411 AflIII A'CryG_T Cuts at: 1203 2769 4507 5484 1203 Size: 1566 1738 977 1327 Fragments arranged by size: 1738 1566 1327 977 AgeI A'CCGG_T Cuts at: 4439 4439 Size: 5608 AhdI GACnn_n'nnGTC Cuts at: 4337 4337 Size: 5608 AleI CACnn'nnGTG Cuts at: 1166 2534 2654 1166 Size: 1368 120 4120 Fragments arranged by size: 4120 1368 120 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5608 AlwNI CAG_nnn'CTG Cuts at: 2807 3351 4923 2807 Size: 544 1572 3492 Fragments arranged by size: 3492 1572 544 ApaLI G'TGCA_C Cuts at: 3665 4821 3665 Size: 1156 4452 ApoI r'AATT_y Cuts at: 1493 2903 2917 3143 3295 5404 1493 Size: 1410 14 226 152 2109 1697 Fragments arranged by size: 2109 1697 1410 226 152 14 AseI AT'TA_AT Cuts at: 160 1032 4162 160 Size: 872 3130 1606 Fragments arranged by size: 3130 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5608 AvaI C'yCGr_G Cuts at: 1870 1943 2933 1870 Size: 73 990 4545 Fragments arranged by size: 4545 990 73 BamHI G'GATC_C Cuts at: 2938 3305 2938 Size: 367 5241 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2085 2294 2578 2810 2929 Size: 325 217 627 298 209 284 232 119 Cuts at: 2929 4384 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 325 298 284 232 217 209 119 BanII G_rGCy'C Cuts at: 1391 2927 1391 Size: 1536 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4637 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2887 2921 3799 3833 1903 Size: 34 950 34 878 34 3678 Fragments arranged by size: 3678 950 878 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3500 4710 3500 Size: 1210 4398 BclI T'GATC_A Cuts at: 1548 1548 Size: 5608 BfrBI ATG'CAT Cuts at: 2505 2505 Size: 5608 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4219 136 Size: 107 122 71 631 605 2547 1525 Fragments arranged by size: 2547 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2664 5603 2664 Size: 2939 2669 BlpI GC'TnA_GC Cuts at: 2351 3025 2351 Size: 674 4934 Bme1580I G_kGCm'C Cuts at: 1790 2583 3669 4825 1790 Size: 793 1086 1156 2573 Fragments arranged by size: 2573 1156 1086 793 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5608 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3339 4297 442 Size: 416 53 83 399 24 1922 958 1753 Fragments arranged by size: 1922 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2157 1056 Size: 1101 4507 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2759 4268 1239 Size: 1520 1509 2579 Fragments arranged by size: 2579 1520 1509 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2182 2330 3733 4598 4896 2182 Size: 148 1403 865 298 2894 Fragments arranged by size: 2894 1403 865 298 148 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4271 882 Size: 617 2772 2219 Fragments arranged by size: 2772 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3399 493 Size: 711 2195 2702 Fragments arranged by size: 2702 2195 711 BsaBI GATnn'nnATC Cuts at: 5602 5602 Size: 5608 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3415 3797 4432 275 Size: 53 83 186 936 1882 382 635 1451 Fragments arranged by size: 1882 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4041 4439 4713 4860 5271 4041 Size: 398 274 147 411 4378 Fragments arranged by size: 4378 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5383 Fragments arranged by size: 5383 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2220 1186 Size: 1034 4574 BseYI C'CCAG_C Cuts at: 2554 4811 5307 2554 Size: 2257 496 2855 Fragments arranged by size: 2855 2257 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2453 2475 2820 2881 1163 Size: 573 717 22 345 61 3890 Fragments arranged by size: 3890 717 573 345 61 22 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3058 3819 4847 1540 Size: 275 186 1057 761 1028 2301 Fragments arranged by size: 2301 1057 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3669 3754 4825 3669 Size: 85 1071 4452 Fragments arranged by size: 4452 1071 85 BsmI GAATG_Cn' Cuts at: 2189 2710 3119 3212 2189 Size: 521 409 93 4585 Fragments arranged by size: 4585 521 409 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2583 2927 3669 3754 4825 1391 Size: 399 793 344 742 85 1071 2174 Fragments arranged by size: 2174 1071 793 742 399 344 85 BspEI T'CCGG_A Cuts at: 5271 5271 Size: 5608 BspHI T'CATG_A Cuts at: 3497 5227 3497 Size: 1730 3878 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2073 2963 844 Size: 1229 890 3489 Fragments arranged by size: 3489 1229 890 BsrBI CCG'CTC Cuts at: 1394 1823 3495 1394 Size: 429 1672 3507 Fragments arranged by size: 3507 1672 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2820 4103 4277 65 Size: 1183 1572 1283 174 1396 Fragments arranged by size: 1572 1396 1283 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4252 4439 1700 Size: 110 2442 187 2869 Fragments arranged by size: 2869 2442 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5608 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5608 BssSI C'ACGA_G Cuts at: 3668 4680 3668 Size: 1012 4596 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5608 BstBI TT'CG_AA Cuts at: 4412 4412 Size: 5608 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5608 BstXI CCAn_nnnn'nTGG Cuts at: 2656 2656 Size: 5608 BstYI r'GATC_y Cuts at: 1972 2664 2938 3305 3690 3707 5159 5245 5257 Size: 692 274 367 385 17 1452 86 12 Cuts at: 5257 5603 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 692 385 367 346 274 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2298 2649 513 Size: 552 939 294 351 3472 Fragments arranged by size: 3472 939 552 351 294 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4869 Fragments arranged by size: 4869 683 56 BtsI GCAGTG_nn' Cuts at: 2463 3124 3918 3938 2463 Size: 661 794 20 4133 Fragments arranged by size: 4133 794 661 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5608 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2803 2838 529 Size: 35 2239 35 3299 Fragments arranged by size: 3299 2239 35 35 DraI TTT'AAA Cuts at: 2482 2912 2482 Size: 430 5178 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4615 809 Size: 507 3299 1802 Fragments arranged by size: 3299 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2229 2497 3055 Size: 54 1425 50 275 18 399 268 558 Cuts at: 3055 3944 5573 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 558 399 275 268 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3055 1537 Size: 275 1243 4090 Fragments arranged by size: 4090 1243 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3538 1353 Size: 2185 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4195 4569 4715 387 Size: 1064 138 2606 374 146 1280 Fragments arranged by size: 2606 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2759 3671 4268 5055 5323 1239 Size: 393 1127 912 597 787 268 1524 Fragments arranged by size: 1524 1127 912 787 597 393 268 EcoRI G'AATT_C Cuts at: 2917 3295 2917 Size: 378 5230 EcoRV GAT'ATC Cuts at: 2677 2677 Size: 5608 FspI TGC'GCA Cuts at: 4114 4114 Size: 5608 HaeII r_GCGC'y Cuts at: 1678 4755 1678 Size: 3077 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4249 4281 4323 4355 1373 Size: 32 135 32 2677 32 42 32 2626 Fragments arranged by size: 2677 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2610 2952 3198 669 Size: 988 281 672 342 246 3079 Fragments arranged by size: 3079 988 672 342 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5608 HpaI GTT'AAC Cuts at: 3198 3198 Size: 5608 KpnI G_GTAC'C Cuts at: 2089 2933 2089 Size: 844 4764 MfeI C'AATT_G Cuts at: 3207 3207 Size: 5608 MluI A'CGCG_T Cuts at: 5484 5484 Size: 5608 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2704 4722 4906 1305 Size: 1399 2018 184 2007 Fragments arranged by size: 2018 2007 1399 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2231 10 Size: 54 1425 343 399 3387 Fragments arranged by size: 3387 1425 399 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2534 2654 3566 3925 4084 518 Size: 648 1368 120 912 359 159 2042 Fragments arranged by size: 2042 1368 912 648 359 159 120 MspA1I CmG'CkG Cuts at: 1606 2576 3024 3701 4849 5094 1606 Size: 970 448 677 1148 245 2120 Fragments arranged by size: 2120 1148 970 677 448 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4117 Fragments arranged by size: 4117 939 552 NdeI CA'TA_TG Cuts at: 387 2603 387 Size: 2216 3392 NheI G'CTAG_C Cuts at: 1052 2153 1052 Size: 1101 4507 NotI GC'GGCC_GC Cuts at: 3055 3055 Size: 5608 NsiI A_TGCA'T Cuts at: 2507 2507 Size: 5608 NspI r_CATG'y Cuts at: 1132 1229 2282 2773 2966 4511 1132 Size: 97 1053 491 193 1545 2229 Fragments arranged by size: 2229 1545 1053 491 193 97 PasI CC'CwG_GG Cuts at: 1348 2140 1348 Size: 792 4816 PciI A'CATG_T Cuts at: 2769 4507 2769 Size: 1738 3870 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5608 PmeI GTTT'AAAC Cuts at: 2912 2912 Size: 5608 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3677 3709 5190 5222 3677 Size: 32 1481 32 4063 Fragments arranged by size: 4063 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5608 PsiI TTA'TAA Cuts at: 3178 3178 Size: 5608 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2105 2137 2105 Size: 32 5576 PstI C_TGCA'G Cuts at: 830 2017 2960 830 Size: 1187 943 3478 Fragments arranged by size: 3478 1187 943 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5608 SalI G'TCGA_C Cuts at: 1936 2950 1936 Size: 1014 4594 SbfI CC_TGCA'GG Cuts at: 2960 2960 Size: 5608 ScaI AGT'ACT Cuts at: 3856 5568 3856 Size: 1712 3896 SfcI C'TryA_G Cuts at: 826 1045 2013 2956 4091 4772 4963 826 Size: 219 968 943 1135 681 191 1471 Fragments arranged by size: 1471 1135 968 943 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5608 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5608 SmaI CCC'GGG Cuts at: 1872 2935 1872 Size: 1063 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2161 2309 3712 4613 4875 820 Size: 197 926 218 148 1403 901 262 1553 Fragments arranged by size: 1553 1403 926 901 262 218 197 148 SnaBI TAC'GTA Cuts at: 493 3399 493 Size: 2906 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5608 SphI G_CATG'C Cuts at: 1132 2966 1132 Size: 1834 3774 SspI AAT'ATT Cuts at: 5 52 3532 5 Size: 47 3480 2081 Fragments arranged by size: 3480 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5608 StyI C'CwwG_G Cuts at: 513 1065 2004 2699 3047 513 Size: 552 939 695 348 3074 Fragments arranged by size: 3074 939 695 552 348 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2546 3635 3652 3805 3990 4435 325 Size: 1255 966 1089 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1089 966 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3854 5566 96 Size: 274 80 33 51 1443 1877 1712 138 Fragments arranged by size: 1877 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2638 2670 5568 5600 2638 Size: 32 2898 32 2646 Fragments arranged by size: 2898 2646 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5608 XbaI T'CTAG_A Cuts at: 2944 2944 Size: 5608 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5608 XmaI C'CCGG_G Cuts at: 1870 2933 1870 Size: 1063 4545 XmnI GAAnn'nnTTC Cuts at: 2708 3737 2708 Size: 1029 4579 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3416 276 Size: 53 83 186 936 1882 2468 Fragments arranged by size: 2468 1882 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI