(Circular) (Six-base) MAPSORT of: pFN21AE0002.seq Check: 6998 from: 1 to: 6442 pFN21AE0002 6442 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2104 2213 2104 Size: 109 6333 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4252 278 Size: 53 83 186 936 2716 2468 Fragments arranged by size: 2716 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3415 3785 1937 Size: 1478 370 4594 Fragments arranged by size: 4594 1478 370 Acc65I G'GTAC_C Cuts at: 3763 3763 Size: 6442 AclI AA'CG_TT Cuts at: 1563 4569 4942 1563 Size: 3006 373 3063 Fragments arranged by size: 3063 3006 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2766 4505 5889 6157 1632 Size: 1134 1739 1384 268 1917 Fragments arranged by size: 1917 1739 1384 1134 268 AfeI AGC'GCT Cuts at: 2727 2727 Size: 6442 AflII C'TTAA_G Cuts at: 820 1017 2729 820 Size: 197 1712 4533 Fragments arranged by size: 4533 1712 197 AflIII A'CryG_T Cuts at: 1203 5341 6318 1203 Size: 4138 977 1327 Fragments arranged by size: 4138 1327 977 AgeI A'CCGG_T Cuts at: 5273 5273 Size: 6442 AhdI GACnn_n'nnGTC Cuts at: 5171 5171 Size: 6442 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6442 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3179 1184 Size: 1995 4447 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2510 2542 2510 Size: 32 6410 AlwNI CAG_nnn'CTG Cuts at: 2119 2233 2515 2695 2764 4185 5757 2119 Size: 114 282 180 69 1421 1572 2804 Fragments arranged by size: 2804 1572 1421 282 180 114 69 ApaI G_GGCC'C Cuts at: 2128 2933 3223 3392 2128 Size: 805 290 169 5178 Fragments arranged by size: 5178 805 290 169 ApaLI G'TGCA_C Cuts at: 4499 5655 4499 Size: 1156 5286 ApoI r'AATT_y Cuts at: 1493 2321 3751 3977 4129 6238 1493 Size: 828 1430 226 152 2109 1697 Fragments arranged by size: 2109 1697 1430 828 226 152 AseI AT'TA_AT Cuts at: 160 1032 4996 160 Size: 872 3964 1606 Fragments arranged by size: 3964 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6442 AvaI C'yCGr_G Cuts at: 1870 1943 2069 2127 2676 3090 3365 3385 3738 Size: 73 126 58 549 414 275 20 353 Cuts at: 3738 3767 1870 Size: 29 4545 Fragments arranged by size: 4545 549 414 353 275 126 73 58 29 20 BamHI G'GATC_C Cuts at: 3772 4139 3772 Size: 367 6075 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2734 3058 3155 3283 3763 Size: 325 217 627 947 324 97 128 480 Cuts at: 3763 5218 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 947 627 480 325 324 217 128 97 BanII G_rGCy'C Cuts at: 1391 2128 2933 3082 3186 3223 3341 3372 3392 Size: 737 805 149 104 37 118 31 20 Cuts at: 3392 3761 1391 Size: 369 4072 Fragments arranged by size: 4072 805 737 369 149 118 104 37 31 20 BbeI G_GCGC'C Cuts at: 3287 3287 Size: 6442 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5471 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4633 4667 1903 Size: 34 2696 34 3678 Fragments arranged by size: 3678 2696 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4334 5544 4334 Size: 1210 5232 BclI T'GATC_A Cuts at: 1548 2235 1548 Size: 687 5755 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3577 5053 136 Size: 107 122 71 631 605 1905 1476 1525 Fragments arranged by size: 1905 1525 1476 631 605 122 107 71 BglII A'GATC_T Cuts at: 2973 6437 2973 Size: 3464 2978 BlpI GC'TnA_GC Cuts at: 3859 3859 Size: 6442 Bme1580I G_kGCm'C Cuts at: 1790 2128 2509 2933 3223 3392 3637 4503 5659 Size: 338 381 424 290 169 245 866 1156 Cuts at: 5659 1790 Size: 2573 Fragments arranged by size: 2573 1156 866 424 381 338 290 245 169 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6442 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3470 4173 5131 Size: 416 53 83 399 24 2053 703 958 Cuts at: 5131 442 Size: 1753 Fragments arranged by size: 2053 1753 958 703 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6442 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2365 2365 Size: 6442 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2360 2537 2717 2891 2902 2909 3296 3531 Size: 1121 177 180 174 11 7 387 235 Cuts at: 3531 5102 1239 Size: 1571 2579 Fragments arranged by size: 2579 1571 1121 387 235 180 177 174 11 7 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3356 4567 5432 5730 3356 Size: 1211 865 298 4068 Fragments arranged by size: 4068 1211 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2099 2906 5105 882 Size: 617 600 807 2199 2219 Fragments arranged by size: 2219 2199 807 617 600 BsaAI yAC'GTr Cuts at: 493 1204 4233 493 Size: 711 3029 2702 Fragments arranged by size: 3029 2702 711 BsaBI GATnn'nnATC Cuts at: 6436 6436 Size: 6442 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3267 3284 3381 3412 Size: 53 83 186 936 1734 17 97 31 Cuts at: 3412 4249 4631 5266 275 Size: 837 382 635 1451 Fragments arranged by size: 1734 1451 936 837 635 382 186 97 83 53 31 17 BsaWI w'CCGG_w Cuts at: 3296 4875 5273 5547 5694 6105 3296 Size: 1579 398 274 147 411 3633 Fragments arranged by size: 3633 1579 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2267 2297 2510 2540 3408 Size: 30 165 30 864 30 213 30 868 Cuts at: 3408 3438 3646 3676 1178 Size: 30 208 30 3944 Fragments arranged by size: 3944 868 864 213 208 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3143 3146 3564 3636 1186 Size: 1957 3 418 72 3992 Fragments arranged by size: 3992 1957 418 72 3 BseYI C'CCAG_C Cuts at: 2119 2358 2373 2409 3545 3562 5645 6141 2119 Size: 239 15 36 1136 17 2083 496 2420 Fragments arranged by size: 2420 2083 1136 496 239 36 17 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2121 2522 2708 3149 1163 Size: 573 385 401 186 441 4456 Fragments arranged by size: 4456 573 441 401 385 186 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2398 2955 3150 3348 3456 3892 Size: 275 186 397 557 195 198 108 436 Cuts at: 3892 4653 5681 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 761 557 436 397 275 198 195 186 108 BsiHKAI G_wGCw'C Cuts at: 2178 3186 3372 4503 4588 5659 2178 Size: 1008 186 1131 85 1071 2961 Fragments arranged by size: 2961 1131 1071 1008 186 85 BsmI GAATG_Cn' Cuts at: 3953 4046 3953 Size: 93 6349 BsmBI CGTCTCn'nnnn_ Cuts at: 2777 2777 Size: 6442 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2128 2178 2509 2933 3082 3186 3223 Size: 399 338 50 331 424 149 104 37 Cuts at: 3223 3341 3372 3392 3637 3761 4503 4588 5659 Size: 118 31 20 245 124 742 85 1071 Cuts at: 5659 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 424 399 338 331 245 149 124 118 104 85 50 37 31 20 BspEI T'CCGG_A Cuts at: 6105 6105 Size: 6442 BspHI T'CATG_A Cuts at: 4331 6061 4331 Size: 1730 4712 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2104 2213 2577 3003 3171 3343 3797 844 Size: 1260 109 364 426 168 172 454 3489 Fragments arranged by size: 3489 1260 454 426 364 172 168 109 BsrBI CCG'CTC Cuts at: 1394 1823 3136 4329 1394 Size: 429 1313 1193 3507 Fragments arranged by size: 3507 1313 1193 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4937 5111 65 Size: 1183 3689 174 1396 Fragments arranged by size: 3689 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2737 3105 3168 3312 5086 5273 1700 Size: 110 927 368 63 144 1774 187 2869 Fragments arranged by size: 2869 1774 927 368 187 144 110 63 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6442 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6442 BssSI C'ACGA_G Cuts at: 4502 5514 4502 Size: 1012 5430 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6442 BstBI TT'CG_AA Cuts at: 5246 5246 Size: 6442 BstEII G'GTnAC_C Cuts at: 1184 2086 3121 1184 Size: 902 1035 4505 Fragments arranged by size: 4505 1035 902 BstYI r'GATC_y Cuts at: 1972 2973 3772 4139 4524 4541 5993 6079 6091 Size: 1001 799 367 385 17 1452 86 12 Cuts at: 6091 6437 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1001 799 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3435 513 Size: 552 2370 3520 Fragments arranged by size: 3520 2370 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5703 Fragments arranged by size: 5703 683 56 BtsI GCAGTG_nn' Cuts at: 3637 3958 4752 4772 3637 Size: 321 794 20 5307 Fragments arranged by size: 5307 794 321 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6442 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6407 DraI TTT'AAA Cuts at: 3746 3746 Size: 6442 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2956 5449 809 Size: 507 1640 2493 1802 Fragments arranged by size: 2493 1802 1640 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2395 2952 3147 Size: 54 1425 50 275 18 565 557 195 Cuts at: 3147 3345 3432 3438 3453 3889 4778 6407 8 Size: 198 87 6 15 436 889 1629 43 Fragments arranged by size: 1629 1425 889 565 557 436 275 198 195 87 54 50 43 18 15 6 EagI C'GGCC_G Cuts at: 1537 1812 2395 2952 3147 3345 3453 3889 1537 Size: 275 583 557 195 198 108 436 4090 Fragments arranged by size: 4090 583 557 436 275 198 195 108 EarI CTCTTCn'nnn_ Cuts at: 1353 2384 3090 4372 1353 Size: 1031 706 1282 3423 Fragments arranged by size: 3423 1282 1031 706 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2510 2633 3010 5029 5403 5549 Size: 1064 138 921 123 377 2019 374 146 Cuts at: 5549 387 Size: 1280 Fragments arranged by size: 2019 1280 1064 921 377 374 146 138 123 EcoICRI GAG'CTC Cuts at: 3184 3370 3184 Size: 186 6256 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2360 2537 2717 2766 2891 2902 2909 Size: 393 728 177 180 49 125 11 7 Cuts at: 2909 3296 3531 4505 5102 5889 6157 1239 Size: 387 235 974 597 787 268 1524 Fragments arranged by size: 1524 974 787 728 597 393 387 268 235 180 177 125 49 11 7 EcoNI CCTnn'n_nnAGG Cuts at: 2270 2270 Size: 6442 EcoO109I rG'GnC_Cy Cuts at: 2124 2692 2800 2929 2930 3389 2124 Size: 568 108 129 1 459 5177 Fragments arranged by size: 5177 568 459 129 108 1 EcoRI G'AATT_C Cuts at: 3751 4129 3751 Size: 378 6064 FspI TGC'GCA Cuts at: 4948 4948 Size: 6442 HaeII r_GCGC'y Cuts at: 1678 2729 3287 5589 1678 Size: 1051 558 2302 2531 Fragments arranged by size: 2531 2302 1051 558 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2267 2299 2875 2907 3408 Size: 32 135 32 695 32 576 32 501 Cuts at: 3408 3440 5083 5115 5157 5189 1373 Size: 32 1643 32 42 32 2626 Fragments arranged by size: 2626 1643 695 576 501 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3358 3416 3786 4032 669 Size: 988 281 1420 58 370 246 3079 Fragments arranged by size: 3079 1420 988 370 281 246 58 HindIII A'AGCT_T Cuts at: 748 748 Size: 6442 HpaI GTT'AAC Cuts at: 4032 4032 Size: 6442 KasI G'GCGC_C Cuts at: 3283 3283 Size: 6442 KpnI G_GTAC'C Cuts at: 3767 3767 Size: 6442 MfeI C'AATT_G Cuts at: 4041 4041 Size: 6442 MluI A'CGCG_T Cuts at: 6318 6318 Size: 6442 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2460 5556 5740 1305 Size: 1155 3096 184 2007 Fragments arranged by size: 3096 2007 1155 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4620 Fragments arranged by size: 4620 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2966 4400 4759 4918 518 Size: 648 1800 1434 359 159 2042 Fragments arranged by size: 2042 1800 1434 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2280 2533 3263 3504 3858 4535 5683 5928 Size: 674 253 730 241 354 677 1148 245 Cuts at: 5928 1606 Size: 2120 Fragments arranged by size: 2120 1148 730 677 674 354 253 245 241 NaeI GCC'GGC Cuts at: 3107 3107 Size: 6442 NarI GG'CG_CC Cuts at: 3284 3284 Size: 6442 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5890 NdeI CA'TA_TG Cuts at: 387 387 Size: 6442 NgoMIV G'CCGG_C Cuts at: 3105 3105 Size: 6442 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6442 NotI GC'GGCC_GC Cuts at: 3889 3889 Size: 6442 NspI r_CATG'y Cuts at: 1132 1229 3800 5345 1132 Size: 97 2571 1545 2229 Fragments arranged by size: 2571 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6442 PciI A'CATG_T Cuts at: 5341 5341 Size: 6442 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6442 PfoI T'CCnGG_A Cuts at: 2062 2514 2062 Size: 452 5990 PmeI GTTT'AAAC Cuts at: 3746 3746 Size: 6442 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2510 2542 4511 4543 6024 6056 2510 Size: 32 1969 32 1481 32 2896 Fragments arranged by size: 2896 1969 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2800 2800 Size: 6442 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6442 PsiI TTA'TAA Cuts at: 4012 4012 Size: 6442 PspOMI G'GGCC_C Cuts at: 2124 2929 3219 3388 2124 Size: 805 290 169 5178 Fragments arranged by size: 5178 805 290 169 PspXI vC'TCGA_Gb Cuts at: 3738 3738 Size: 6442 PstI C_TGCA'G Cuts at: 830 2616 2652 2756 3000 3794 830 Size: 1786 36 104 244 794 3478 Fragments arranged by size: 3478 1786 794 244 104 36 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6442 SacI G_AGCT'C Cuts at: 3186 3372 3186 Size: 186 6256 SalI G'TCGA_C Cuts at: 1936 3414 3784 1936 Size: 1478 370 4594 Fragments arranged by size: 4594 1478 370 SanDI GG'GwC_CC Cuts at: 2800 2800 Size: 6442 SapI GCTCTTCn'nnn_ Cuts at: 2384 2384 Size: 6442 SbfI CC_TGCA'GG Cuts at: 3000 3794 3000 Size: 794 5648 ScaI AGT'ACT Cuts at: 4690 6402 4690 Size: 1712 4730 SfcI C'TryA_G Cuts at: 826 1045 2612 2648 2752 2996 3419 3790 4925 Size: 219 1567 36 104 244 423 371 1135 Cuts at: 4925 5606 5797 826 Size: 681 191 1471 Fragments arranged by size: 1567 1471 1135 681 423 371 244 219 191 104 36 SfiI GGCCn_nnn'nGGCC Cuts at: 3577 3577 Size: 6442 SfoI GGC'GCC Cuts at: 3285 3285 Size: 6442 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6442 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6442 SmaI CCC'GGG Cuts at: 1872 2071 2129 3092 3769 1872 Size: 199 58 963 677 4545 Fragments arranged by size: 4545 963 677 199 58 SmlI C'TyrA_G Cuts at: 820 1017 1943 2729 3371 3738 4546 5447 5709 Size: 197 926 786 642 367 808 901 262 Cuts at: 5709 820 Size: 1553 Fragments arranged by size: 1553 926 901 808 786 642 367 262 197 SnaBI TAC'GTA Cuts at: 493 4233 493 Size: 3740 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6442 SphI G_CATG'C Cuts at: 1132 3800 1132 Size: 2668 3774 SspI AAT'ATT Cuts at: 5 52 4366 5 Size: 47 4314 2081 Fragments arranged by size: 4314 2081 47 StuI AGG'CCT Cuts at: 1517 2265 1517 Size: 748 5694 StyI C'CwwG_G Cuts at: 513 1065 3881 513 Size: 552 2816 3074 Fragments arranged by size: 3074 2816 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2556 3140 3704 4469 4486 4639 4824 Size: 1255 976 584 564 765 17 153 185 Cuts at: 4824 5269 325 Size: 445 1498 Fragments arranged by size: 1498 1255 976 765 584 564 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2199 2547 4688 Size: 274 80 33 51 1443 222 348 2141 Cuts at: 4688 6400 96 Size: 1712 138 Fragments arranged by size: 2141 1712 1443 348 274 222 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2697 2729 3646 3678 6402 6434 2697 Size: 32 917 32 2724 32 2705 Fragments arranged by size: 2724 2705 917 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6442 XbaI T'CTAG_A Cuts at: 3778 3778 Size: 6442 XhoI C'TCGA_G Cuts at: 1943 3738 1943 Size: 1795 4647 XmaI C'CCGG_G Cuts at: 1870 2069 2127 3090 3767 1870 Size: 199 58 963 677 4545 Fragments arranged by size: 4545 963 677 199 58 XmnI GAAnn'nnTTC Cuts at: 4571 4571 Size: 6442 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4250 276 Size: 53 83 186 936 2716 2468 Fragments arranged by size: 2716 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PsrI PvuII RsrII SacII SexAI SrfI SwaI XcmI