(Circular) (Six-base) MAPSORT of: pFN21AE0312.seq Check: 960 from: 1 to: 6280 pFN21AE0312 6280 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2315 3288 2315 Size: 973 5307 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4090 278 Size: 53 83 186 936 2554 2468 Fragments arranged by size: 2554 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2759 3623 1937 Size: 822 864 4594 Fragments arranged by size: 4594 864 822 Acc65I G'GTAC_C Cuts at: 3366 3601 3366 Size: 235 6045 AclI AA'CG_TT Cuts at: 1563 4407 4780 1563 Size: 2844 373 3063 Fragments arranged by size: 3063 2844 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2558 3422 3540 4343 5727 5995 1632 Size: 926 864 118 803 1384 268 1917 Fragments arranged by size: 1917 1384 926 864 803 268 118 AfeI AGC'GCT Cuts at: 2919 2919 Size: 6280 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6083 AflIII A'CryG_T Cuts at: 1203 5179 6156 1203 Size: 3976 977 1327 Fragments arranged by size: 3976 1327 977 AgeI A'CCGG_T Cuts at: 5111 5111 Size: 6280 AhdI GACnn_n'nnGTC Cuts at: 5009 5009 Size: 6280 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6280 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6280 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3153 3185 3153 Size: 32 6248 AlwNI CAG_nnn'CTG Cuts at: 2222 2735 2820 3289 4023 5595 2222 Size: 513 85 469 734 1572 2907 Fragments arranged by size: 2907 1572 734 513 469 85 ApaI G_GGCC'C Cuts at: 2173 2173 Size: 6280 ApaLI G'TGCA_C Cuts at: 4337 5493 4337 Size: 1156 5124 ApoI r'AATT_y Cuts at: 1493 3589 3815 3967 6076 1493 Size: 2096 226 152 2109 1697 Fragments arranged by size: 2109 2096 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4834 160 Size: 872 3802 1606 Fragments arranged by size: 3802 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6280 AvaI C'yCGr_G Cuts at: 1870 1943 2277 2368 3135 3605 1870 Size: 73 334 91 767 470 4545 Fragments arranged by size: 4545 767 470 334 91 73 BamHI G'GATC_C Cuts at: 3610 3977 3610 Size: 367 5913 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3215 3310 3366 3601 5056 Size: 325 217 627 1428 95 56 235 1455 Cuts at: 5056 618 Size: 1842 Fragments arranged by size: 1842 1455 1428 627 325 235 217 95 56 BanII G_rGCy'C Cuts at: 1391 2173 2465 2985 3432 3599 1391 Size: 782 292 520 447 167 4072 Fragments arranged by size: 4072 782 520 447 292 167 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5309 BbvCI CC'TCA_GC Cuts at: 2223 3276 2223 Size: 1053 5227 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4471 4505 1903 Size: 34 2534 34 3678 Fragments arranged by size: 3678 2534 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2072 4172 5382 2072 Size: 2100 1210 2970 Fragments arranged by size: 2970 2100 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6280 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3213 3394 4891 Size: 107 122 71 631 605 1541 181 1497 Cuts at: 4891 136 Size: 1525 Fragments arranged by size: 1541 1525 1497 631 605 181 122 107 71 BglII A'GATC_T Cuts at: 6275 6275 Size: 6280 BlpI GC'TnA_GC Cuts at: 3697 3697 Size: 6280 Bme1580I G_kGCm'C Cuts at: 1790 2173 3218 4341 5497 1790 Size: 383 1045 1123 1156 2573 Fragments arranged by size: 2573 1156 1123 1045 383 BmgBI CAC'GTC Cuts at: 1318 2932 1318 Size: 1614 4666 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3025 3091 4011 Size: 416 53 83 399 24 1608 66 920 Cuts at: 4011 4969 442 Size: 958 1753 Fragments arranged by size: 1753 1608 958 920 416 399 83 66 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6280 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2887 2887 Size: 6280 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2651 4940 1239 Size: 1412 2289 2579 Fragments arranged by size: 2579 2289 1412 Bpu10I CC'TnA_GC Cuts at: 2163 2223 3276 3304 2163 Size: 60 1053 28 5139 Fragments arranged by size: 5139 1053 60 28 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2325 2969 4405 5270 5568 2325 Size: 644 1436 865 298 3037 Fragments arranged by size: 3037 1436 865 644 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4943 882 Size: 617 3444 2219 Fragments arranged by size: 3444 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4071 493 Size: 711 2867 2702 Fragments arranged by size: 2867 2702 711 BsaBI GATnn'nnATC Cuts at: 6274 6274 Size: 6280 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4087 4469 5104 275 Size: 53 83 186 936 2554 382 635 1451 Fragments arranged by size: 2554 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4713 5111 5385 5532 5943 4713 Size: 398 274 147 411 5050 Fragments arranged by size: 5050 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3153 3183 1178 Size: 30 165 30 1750 30 4275 Fragments arranged by size: 4275 1750 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2042 2045 2984 2987 1186 Size: 856 3 939 3 4479 Fragments arranged by size: 4479 939 856 3 3 BseYI C'CCAG_C Cuts at: 2215 2655 3270 5483 5979 2215 Size: 440 615 2213 496 2516 Fragments arranged by size: 2516 2213 615 496 440 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5707 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3730 4491 5519 1540 Size: 275 186 1729 761 1028 2301 Fragments arranged by size: 2301 1729 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2985 3305 4341 4426 5497 2985 Size: 320 1036 85 1071 3768 Fragments arranged by size: 3768 1071 1036 320 85 BsmI GAATG_Cn' Cuts at: 3791 3884 3791 Size: 93 6187 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2173 2465 2985 3218 3305 3432 3599 Size: 399 383 292 520 233 87 127 167 Cuts at: 3599 4341 4426 5497 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 520 399 383 292 233 167 127 87 85 BspEI T'CCGG_A Cuts at: 5943 5943 Size: 6280 BspHI T'CATG_A Cuts at: 4169 5899 4169 Size: 1730 4550 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2315 2449 3288 3635 844 Size: 1471 134 839 347 3489 Fragments arranged by size: 3489 1471 839 347 134 BsrBI CCG'CTC Cuts at: 1394 1823 2058 2167 2186 2947 3262 4167 1394 Size: 429 235 109 19 761 315 905 3507 Fragments arranged by size: 3507 905 761 429 315 235 109 19 BsrDI GCAATG_nn' Cuts at: 65 1248 2710 3569 4775 4949 65 Size: 1183 1462 859 1206 174 1396 Fragments arranged by size: 1462 1396 1206 1183 859 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2151 2376 4924 5111 1700 Size: 110 341 225 2548 187 2869 Fragments arranged by size: 2869 2548 341 225 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6280 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6280 BssSI C'ACGA_G Cuts at: 4340 5352 4340 Size: 1012 5268 BstAPI GCAn_nnn'nTGC Cuts at: 794 2310 794 Size: 1516 4764 BstBI TT'CG_AA Cuts at: 5084 5084 Size: 6280 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6280 BstYI r'GATC_y Cuts at: 1972 3610 3977 4362 4379 5831 5917 5929 6275 Size: 1638 367 385 17 1452 86 12 346 Cuts at: 6275 1972 Size: 1977 Fragments arranged by size: 1977 1638 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2760 2760 Size: 6280 Bsu36I CC'TnA_GG Cuts at: 2832 3315 2832 Size: 483 5797 BtgI C'CryG_G Cuts at: 513 1065 2004 3370 513 Size: 552 939 1366 3423 Fragments arranged by size: 3423 1366 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5541 Fragments arranged by size: 5541 683 56 BtsI GCAGTG_nn' Cuts at: 3479 3796 4590 4610 3479 Size: 317 794 20 5149 Fragments arranged by size: 5149 794 317 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6280 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6245 DraI TTT'AAA Cuts at: 3584 3584 Size: 6280 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5287 809 Size: 507 3971 1802 Fragments arranged by size: 3971 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2250 3334 3727 Size: 54 1425 50 275 18 420 1084 393 Cuts at: 3727 4616 6245 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1084 889 420 393 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3727 1537 Size: 275 1915 4090 Fragments arranged by size: 4090 1915 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2112 2742 2847 2856 3398 4210 1353 Size: 759 630 105 9 542 812 3423 Fragments arranged by size: 3423 812 759 630 542 105 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2039 3262 3476 4867 5241 5387 Size: 1064 138 450 1223 214 1391 374 146 Cuts at: 5387 387 Size: 1280 Fragments arranged by size: 1391 1280 1223 1064 450 374 214 146 138 EcoICRI GAG'CTC Cuts at: 2983 2983 Size: 6280 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2558 2651 3422 3540 4343 4940 5727 Size: 393 926 93 771 118 803 597 787 Cuts at: 5727 5995 1239 Size: 268 1524 Fragments arranged by size: 1524 926 803 787 771 597 393 268 118 93 EcoNI CCTnn'n_nnAGG Cuts at: 3535 3535 Size: 6280 EcoO109I rG'GnC_Cy Cuts at: 2170 3286 3319 3408 2170 Size: 1116 33 89 5042 Fragments arranged by size: 5042 1116 89 33 EcoRI G'AATT_C Cuts at: 3589 3967 3589 Size: 378 5902 FspI TGC'GCA Cuts at: 4786 4786 Size: 6280 HaeII r_GCGC'y Cuts at: 1678 2012 2698 2921 3229 5427 1678 Size: 334 686 223 308 2198 2531 Fragments arranged by size: 2531 2198 686 334 308 223 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3129 3161 3551 3583 4921 Size: 32 135 32 1557 32 390 32 1338 Cuts at: 4921 4953 4995 5027 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1557 1338 390 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3624 3870 669 Size: 988 281 1686 246 3079 Fragments arranged by size: 3079 1686 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6280 HpaI GTT'AAC Cuts at: 3870 3870 Size: 6280 KpnI G_GTAC'C Cuts at: 3370 3605 3370 Size: 235 6045 MfeI C'AATT_G Cuts at: 3879 3879 Size: 6280 MluI A'CGCG_T Cuts at: 6156 6156 Size: 6280 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2480 5394 5578 1305 Size: 1175 2914 184 2007 Fragments arranged by size: 2914 2007 1175 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3336 10 Size: 54 1425 343 1504 2954 Fragments arranged by size: 2954 1504 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2874 3098 3506 4238 4597 4756 518 Size: 648 1708 224 408 732 359 159 2042 Fragments arranged by size: 2042 1708 732 648 408 359 224 159 MspA1I CmG'CkG Cuts at: 1606 2222 2659 3696 4373 5521 5766 1606 Size: 616 437 1037 677 1148 245 2120 Fragments arranged by size: 2120 1148 1037 677 616 437 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3370 513 Size: 552 939 1366 3423 Fragments arranged by size: 3423 1366 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6280 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6280 NotI GC'GGCC_GC Cuts at: 3727 3727 Size: 6280 NspI r_CATG'y Cuts at: 1132 1229 2365 3638 5183 1132 Size: 97 1136 1273 1545 2229 Fragments arranged by size: 2229 1545 1273 1136 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6280 PciI A'CATG_T Cuts at: 5179 5179 Size: 6280 PflMI CCAn_nnn'nTGG Cuts at: 1108 3289 1108 Size: 2181 4099 PmeI GTTT'AAAC Cuts at: 3584 3584 Size: 6280 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3153 3185 4349 4381 5862 5894 3153 Size: 32 1164 32 1481 32 3539 Fragments arranged by size: 3539 1481 1164 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6280 PsiI TTA'TAA Cuts at: 3850 3850 Size: 6280 PspOMI G'GGCC_C Cuts at: 2169 2169 Size: 6280 PstI C_TGCA'G Cuts at: 830 2496 2664 3299 3632 830 Size: 1666 168 635 333 3478 Fragments arranged by size: 3478 1666 635 333 168 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6280 PvuII CAG'CTG Cuts at: 2659 2659 Size: 6280 SacI G_AGCT'C Cuts at: 2985 2985 Size: 6280 SalI G'TCGA_C Cuts at: 1936 3622 1936 Size: 1686 4594 SapI GCTCTTCn'nnn_ Cuts at: 2112 2742 2112 Size: 630 5650 SbfI CC_TGCA'GG Cuts at: 3632 3632 Size: 6280 ScaI AGT'ACT Cuts at: 4528 6240 4528 Size: 1712 4568 SexAI A'CCwGG_T Cuts at: 2046 2046 Size: 6280 SfcI C'TryA_G Cuts at: 826 1045 2492 2660 3295 3383 3628 4763 5444 Size: 219 1447 168 635 88 245 1135 681 Cuts at: 5444 5635 826 Size: 191 1471 Fragments arranged by size: 1471 1447 1135 681 635 245 219 191 168 88 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6280 SgrAI Cr'CCGG_yG Cuts at: 1700 2376 1700 Size: 676 5604 SmaI CCC'GGG Cuts at: 1872 3607 1872 Size: 1735 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2277 2340 2984 4384 5285 5547 Size: 197 926 334 63 644 1400 901 262 Cuts at: 5547 820 Size: 1553 Fragments arranged by size: 1553 1400 926 901 644 334 262 197 63 SnaBI TAC'GTA Cuts at: 493 4071 493 Size: 3578 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6280 SphI G_CATG'C Cuts at: 1132 3638 1132 Size: 2506 3774 SspI AAT'ATT Cuts at: 5 52 4204 5 Size: 47 4152 2081 Fragments arranged by size: 4152 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6280 StyI C'CwwG_G Cuts at: 513 1065 2004 2785 3234 3370 3719 513 Size: 552 939 781 449 136 349 3074 Fragments arranged by size: 3074 939 781 552 449 349 136 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2669 3514 4307 4324 4477 4662 5107 Size: 1255 1089 845 793 17 153 185 445 Cuts at: 5107 325 Size: 1498 Fragments arranged by size: 1498 1255 1089 845 793 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2364 3102 4526 Size: 274 80 33 51 1443 387 738 1424 Cuts at: 4526 6238 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1424 738 387 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6240 6272 6240 Size: 32 6248 Tth111I GACn'n_nGTC Cuts at: 1316 3143 3475 1316 Size: 1827 332 4121 Fragments arranged by size: 4121 1827 332 XbaI T'CTAG_A Cuts at: 3616 3616 Size: 6280 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2584 2584 Size: 6280 XhoI C'TCGA_G Cuts at: 1943 2277 1943 Size: 334 5946 XmaI C'CCGG_G Cuts at: 1870 3605 1870 Size: 1735 4545 XmnI GAAnn'nnTTC Cuts at: 4409 4409 Size: 6280 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4088 276 Size: 53 83 186 936 2554 2468 Fragments arranged by size: 2554 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI