(Circular) (Six-base) MAPSORT of: pFN21AB6507.seq Check: 6802 from: 1 to: 5782 pFN21AB6507 5782 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3592 278 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2241 3125 1937 Size: 304 884 4594 Fragments arranged by size: 4594 884 304 Acc65I G'GTAC_C Cuts at: 3103 3103 Size: 5782 AclI AA'CG_TT Cuts at: 1563 3909 4282 1563 Size: 2346 373 3063 Fragments arranged by size: 3063 2346 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2954 3845 5229 5497 1632 Size: 1322 891 1384 268 1917 Fragments arranged by size: 1917 1384 1322 891 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5585 AflIII A'CryG_T Cuts at: 1203 2990 4681 5658 1203 Size: 1787 1691 977 1327 Fragments arranged by size: 1787 1691 1327 977 AgeI A'CCGG_T Cuts at: 4613 4613 Size: 5782 AhdI GACnn_n'nnGTC Cuts at: 4511 4511 Size: 5782 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5782 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5782 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2660 2692 2660 Size: 32 5750 AlwNI CAG_nnn'CTG Cuts at: 3525 5097 3525 Size: 1572 4210 ApaLI G'TGCA_C Cuts at: 3839 4995 3839 Size: 1156 4626 ApoI r'AATT_y Cuts at: 1493 3091 3317 3469 5578 1493 Size: 1598 226 152 2109 1697 Fragments arranged by size: 2109 1697 1598 226 152 AseI AT'TA_AT Cuts at: 160 1032 4336 160 Size: 872 3304 1606 Fragments arranged by size: 3304 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 AvaI C'yCGr_G Cuts at: 1870 1943 2419 2882 3107 1870 Size: 73 476 463 225 4545 Fragments arranged by size: 4545 476 463 225 73 BamHI G'GATC_C Cuts at: 2652 3112 3479 2652 Size: 460 367 4955 Fragments arranged by size: 4955 460 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2195 3103 4558 618 Size: 325 217 627 408 908 1455 1842 Fragments arranged by size: 1842 1455 908 627 408 325 217 BanII G_rGCy'C Cuts at: 1391 2124 3101 1391 Size: 733 977 4072 Fragments arranged by size: 4072 977 733 BbeI G_GCGC'C Cuts at: 2199 2199 Size: 5782 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4811 BbvCI CC'TCA_GC Cuts at: 2699 2699 Size: 5782 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3973 4007 1903 Size: 34 2036 34 3678 Fragments arranged by size: 3678 2036 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3674 4884 3674 Size: 1210 4572 BclI T'GATC_A Cuts at: 1548 1548 Size: 5782 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2267 2915 4393 Size: 107 122 71 631 605 595 648 1478 Cuts at: 4393 136 Size: 1525 Fragments arranged by size: 1525 1478 648 631 605 595 122 107 71 BglII A'GATC_T Cuts at: 5777 5777 Size: 5782 BlpI GC'TnA_GC Cuts at: 3199 3199 Size: 5782 Bme1580I G_kGCm'C Cuts at: 1790 2559 3843 4999 1790 Size: 769 1284 1156 2573 Fragments arranged by size: 2573 1284 1156 769 BmgBI CAC'GTC Cuts at: 1318 2356 2539 1318 Size: 1038 183 4561 Fragments arranged by size: 4561 1038 183 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3513 4471 442 Size: 416 53 83 399 24 2096 958 1753 Fragments arranged by size: 2096 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5782 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2033 2612 2861 4442 1239 Size: 794 579 249 1581 2579 Fragments arranged by size: 2579 1581 794 579 249 Bpu10I CC'TnA_GC Cuts at: 2036 2699 2036 Size: 663 5119 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2108 2279 3047 3907 4772 5070 2108 Size: 171 768 860 865 298 2820 Fragments arranged by size: 2820 865 860 768 298 171 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4445 882 Size: 617 2946 2219 Fragments arranged by size: 2946 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2991 3573 493 Size: 711 1787 582 2702 Fragments arranged by size: 2702 1787 711 582 BsaBI GATnn'nnATC Cuts at: 2183 5776 2183 Size: 3593 2189 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2109 2196 2907 3589 Size: 53 83 186 936 576 87 711 682 Cuts at: 3589 3971 4606 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 711 682 635 576 382 186 87 83 53 BsaWI w'CCGG_w Cuts at: 2607 2742 2769 4215 4613 4887 5034 5445 2607 Size: 135 27 1446 398 274 147 411 2944 Fragments arranged by size: 2944 1446 411 398 274 147 135 27 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2447 2477 1178 Size: 30 165 30 1044 30 4483 Fragments arranged by size: 4483 1044 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2225 2765 2794 1186 Size: 1039 540 29 4174 Fragments arranged by size: 4174 1039 540 29 BseYI C'CCAG_C Cuts at: 2529 2898 4985 5481 2529 Size: 369 2087 496 2830 Fragments arranged by size: 2830 2087 496 369 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5209 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2443 2636 3033 3232 3993 5021 Size: 275 186 442 193 397 199 761 1028 Cuts at: 5021 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 442 397 275 199 193 186 BsiHKAI G_wGCw'C Cuts at: 2124 2165 3843 3928 4999 2124 Size: 41 1678 85 1071 2907 Fragments arranged by size: 2907 1678 1071 85 41 BsmI GAATG_Cn' Cuts at: 2438 3293 3386 2438 Size: 855 93 4834 Fragments arranged by size: 4834 855 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2282 2282 Size: 5782 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2124 2165 2559 3101 3843 3928 4999 Size: 399 334 41 394 542 742 85 1071 Cuts at: 4999 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 542 399 394 334 85 41 BspEI T'CCGG_A Cuts at: 2607 2769 5445 2607 Size: 162 2676 2944 Fragments arranged by size: 2944 2676 162 BspHI T'CATG_A Cuts at: 3671 5401 3671 Size: 1730 4052 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2529 2844 3137 844 Size: 1685 315 293 3489 Fragments arranged by size: 3489 1685 315 293 BsrBI CCG'CTC Cuts at: 1394 1823 2154 2641 3669 1394 Size: 429 331 487 1028 3507 Fragments arranged by size: 3507 1028 487 429 331 BsrDI GCAATG_nn' Cuts at: 65 1248 4277 4451 65 Size: 1183 3029 174 1396 Fragments arranged by size: 3029 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2198 2564 4426 4613 1700 Size: 110 388 366 1862 187 2869 Fragments arranged by size: 2869 1862 388 366 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5782 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5782 BssSI C'ACGA_G Cuts at: 3842 4854 3842 Size: 1012 4770 BstAPI GCAn_nnn'nTGC Cuts at: 794 2539 794 Size: 1745 4037 BstBI TT'CG_AA Cuts at: 4586 4586 Size: 5782 BstEII G'GTnAC_C Cuts at: 1184 2365 1184 Size: 1181 4601 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5782 BstYI r'GATC_y Cuts at: 1972 2298 2652 2850 3112 3479 3864 3881 5333 Size: 326 354 198 262 367 385 17 1452 Cuts at: 5333 5419 5431 5777 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 354 346 326 262 198 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2191 2283 513 Size: 552 1126 92 4012 Fragments arranged by size: 4012 1126 552 92 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2055 2488 487 Size: 683 56 829 433 3781 Fragments arranged by size: 3781 829 683 433 56 BtsI GCAGTG_nn' Cuts at: 2656 3298 4092 4112 2656 Size: 642 794 20 4326 Fragments arranged by size: 4326 794 642 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5782 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5747 DraI TTT'AAA Cuts at: 3086 3086 Size: 5782 DraIII CAC_nnn'GTG Cuts at: 2869 2869 Size: 5782 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2014 4789 809 Size: 507 698 2775 1802 Fragments arranged by size: 2775 1802 698 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3030 3229 4118 Size: 54 1425 50 275 18 1200 199 889 Cuts at: 4118 5747 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1200 889 275 199 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3030 3229 1537 Size: 275 1218 199 4090 Fragments arranged by size: 4090 1218 275 199 EarI CTCTTCn'nnn_ Cuts at: 1353 2153 2822 3712 1353 Size: 800 669 890 3423 Fragments arranged by size: 3423 890 800 669 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4369 4743 4889 387 Size: 1064 138 2780 374 146 1280 Fragments arranged by size: 2780 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2122 2122 Size: 5782 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2033 2612 2861 2954 3845 4442 5229 Size: 393 401 579 249 93 891 597 787 Cuts at: 5229 5497 1239 Size: 268 1524 Fragments arranged by size: 1524 891 787 597 579 401 393 268 249 93 EcoNI CCTnn'n_nnAGG Cuts at: 2411 2840 2411 Size: 429 5353 EcoO109I rG'GnC_Cy Cuts at: 2416 2833 2416 Size: 417 5365 EcoRI G'AATT_C Cuts at: 3091 3469 3091 Size: 378 5404 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2242 2242 Size: 5782 FspI TGC'GCA Cuts at: 2183 4288 2183 Size: 2105 3677 FspAI rTGC'GCAy Cuts at: 2183 2183 Size: 5782 HaeII r_GCGC'y Cuts at: 1678 2199 4929 1678 Size: 521 2730 2531 Fragments arranged by size: 2730 2531 521 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4423 4455 4497 4529 1373 Size: 32 135 32 2851 32 42 32 2626 Fragments arranged by size: 2851 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2497 3126 3372 669 Size: 988 281 559 629 246 3079 Fragments arranged by size: 3079 988 629 559 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5782 HpaI GTT'AAC Cuts at: 3372 3372 Size: 5782 KasI G'GCGC_C Cuts at: 2195 2195 Size: 5782 KpnI G_GTAC'C Cuts at: 3107 3107 Size: 5782 MfeI C'AATT_G Cuts at: 3381 3381 Size: 5782 MluI A'CGCG_T Cuts at: 5658 5658 Size: 5782 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2490 2616 2709 4896 5080 1305 Size: 1185 126 93 2187 184 2007 Fragments arranged by size: 2187 2007 1185 184 126 93 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3960 Fragments arranged by size: 3960 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2261 3740 4099 4258 518 Size: 648 1095 1479 359 159 2042 Fragments arranged by size: 2042 1479 1095 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2089 2632 2902 3198 3875 5023 5268 1606 Size: 483 543 270 296 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 543 483 296 270 245 NaeI GCC'GGC Cuts at: 2200 2200 Size: 5782 NarI GG'CG_CC Cuts at: 2196 2196 Size: 5782 NcoI C'CATG_G Cuts at: 513 1065 2283 513 Size: 552 1218 4012 Fragments arranged by size: 4012 1218 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5782 NgoMIV G'CCGG_C Cuts at: 2198 2198 Size: 5782 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5782 NotI GC'GGCC_GC Cuts at: 3229 3229 Size: 5782 NspI r_CATG'y Cuts at: 1132 1229 3140 4685 1132 Size: 97 1911 1545 2229 Fragments arranged by size: 2229 1911 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5782 PciI A'CATG_T Cuts at: 4681 4681 Size: 5782 PflMI CCAn_nnn'nTGG Cuts at: 1108 2719 1108 Size: 1611 4171 PfoI T'CCnGG_A Cuts at: 2343 2427 2343 Size: 84 5698 PmeI GTTT'AAAC Cuts at: 3086 3086 Size: 5782 PmlI CAC'GTG Cuts at: 2991 2991 Size: 5782 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3851 3883 5364 5396 3851 Size: 32 1481 32 4237 Fragments arranged by size: 4237 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2416 2833 2416 Size: 417 5365 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5782 PsiI TTA'TAA Cuts at: 3352 3352 Size: 5782 PstI C_TGCA'G Cuts at: 830 3054 3134 830 Size: 2224 80 3478 Fragments arranged by size: 3478 2224 80 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5782 PvuII CAG'CTG Cuts at: 2089 2089 Size: 5782 SacI G_AGCT'C Cuts at: 2124 2124 Size: 5782 SalI G'TCGA_C Cuts at: 1936 3124 1936 Size: 1188 4594 SapI GCTCTTCn'nnn_ Cuts at: 2153 2153 Size: 5782 SbfI CC_TGCA'GG Cuts at: 3134 3134 Size: 5782 ScaI AGT'ACT Cuts at: 2480 4030 5742 2480 Size: 1550 1712 2520 Fragments arranged by size: 2520 1712 1550 SexAI A'CCwGG_T Cuts at: 2865 3075 2865 Size: 210 5572 SfcI C'TryA_G Cuts at: 826 1045 3050 3130 4265 4946 5137 826 Size: 219 2005 80 1135 681 191 1471 Fragments arranged by size: 2005 1471 1135 681 219 191 80 SfoI GGC'GCC Cuts at: 2197 2197 Size: 5782 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5782 SmaI CCC'GGG Cuts at: 1872 2421 3109 1872 Size: 549 688 4545 Fragments arranged by size: 4545 688 549 SmlI C'TyrA_G Cuts at: 820 1017 1943 2123 2294 3062 3886 4787 5049 Size: 197 926 180 171 768 824 901 262 Cuts at: 5049 820 Size: 1553 Fragments arranged by size: 1553 926 901 824 768 262 197 180 171 SnaBI TAC'GTA Cuts at: 493 3573 493 Size: 3080 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5782 SphI G_CATG'C Cuts at: 1132 3140 1132 Size: 2008 3774 SspI AAT'ATT Cuts at: 5 52 3706 5 Size: 47 3654 2081 Fragments arranged by size: 3654 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5782 StyI C'CwwG_G Cuts at: 513 1065 2283 2511 3221 513 Size: 552 1218 228 710 3074 Fragments arranged by size: 3074 1218 710 552 228 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2504 2543 3809 3826 3979 4164 4609 Size: 1255 924 39 1266 17 153 185 445 Cuts at: 4609 325 Size: 1498 Fragments arranged by size: 1498 1266 1255 924 445 185 153 39 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2478 2856 3069 Size: 274 80 33 51 1443 501 378 213 Cuts at: 3069 4028 5740 96 Size: 959 1712 138 Fragments arranged by size: 1712 1443 959 501 378 274 213 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5742 5774 5742 Size: 32 5750 Tth111I GACn'n_nGTC Cuts at: 1316 2714 1316 Size: 1398 4384 XbaI T'CTAG_A Cuts at: 3118 3118 Size: 5782 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5782 XmaI C'CCGG_G Cuts at: 1870 2419 3107 1870 Size: 549 688 4545 Fragments arranged by size: 4545 688 549 XmnI GAAnn'nnTTC Cuts at: 2252 2939 3911 2252 Size: 687 972 4123 Fragments arranged by size: 4123 972 687 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3590 276 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I EcoRV FseI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI XcmI