Restriction map for
pFN21AB6414
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AlwI AlwNI AocI ApaI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbsI BcgI BciVI
BclI BfrI BglI BlnI BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstNI BstPI
BstUI BstXI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraII DrdI
Eam1105I Eco81I EcoNI EcoO109I EcoO65I
EcoRII FbaI FseI FspI HgaI
Hin1I HpaI KasI KpnI KspI
MaeII MaeIII MamI MfeI MluI
MroI MslI MunI MvaI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI StuI SwaI Tsp45I
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AfaI 818 GTAC GT^AC
Aor51HI 370 AGCGCT AGC^GCT
AvaII 105 GGTCC G^G[AT]CC
BalI 895 TGGCCA TGG^CCA
BanI 117 GGCACC G^G[CT][GA]CC
BcnI 56 CCGGG CC^[GC]GG
BglII 393 AGATCT A^GATCT
BlpI 50 GCTCAGC GC^T[ACGT]AGC
BpmI 617 CTGGAG CTGGAG(16/14)
Bpu1102I 50 GCTCAGC GC^T[ACGT]AGC
BssKI 56 CCGGG ^CC[ACGT]GG
BstF5I 855 GGATG GGATG(2/0)
BstYI 393 AGATCT [GA]^GATC[CT]
CelII 50 GCTCAGC GC^T[ACGT]AGC
Cfr13I 105 GGTCC G^G[ACGT]CC
DpnII 394 GATC ^GATC
EagI 32 CGGCCG C^GGCCG
EarI 553 GAAGAG CTCTTC(1/4)
EclXI 32 CGGCCG C^GGCCG
Eco47III 370 AGCGCT AGC^GCT
Eco52I 32 CGGCCG C^GGCCG
EcoRI 276 GAATTC G^AATTC
EcoRV 538 GATATC GAT^ATC
EcoT14I 121 CCATGG C^C[AT][AT]GG
EcoT22I 344 ATGCAT ATGCA^T
FauI 7 GCGGG CCCGC(4/6)
FokI 855 GGATG GGATG(9/13)
HaeII 370 AGCGCT [GA]GCGC^[CT]
HapII 56 CCGG C^CGG
HincII 321 GTTGAC GT[CT]^[GA]AC
HindII 321 GTTGAC GT[CT]^[GA]AC
HpaII 56 CCGG C^CGG
HphI 973 GGTGA GGTGA(8/7)
Ksp632I 553 GAAGAG CTCTTC(1/4)
MboI 394 GATC ^GATC
MflI 393 AGATCT [GA]^GATC[CT]
MluNI 895 TGGCCA TGG^CCA
MscI 895 TGGCCA TGG^CCA
MspI 56 CCGG C^CGG
NciI 56 CCGGG CC^[GC]GG
NcoI 121 CCATGG C^CATGG
NdeII 394 GATC ^GATC
NsiI 344 ATGCAT ATGCA^T
PflMI 469 CCACGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 818 GTAC GT^AC
SacI 622 GAGCTC GAGCT^C
Sau3AI 394 GATC ^GATC
Sau96I 105 GGTCC G^G[ACGT]CC
ScaI 817 AGTACT AGT^ACT
ScrFI 56 CCGGG CC^[ACGT]GG
SfcI 26 CTGTAG C^T[GA][CT]AG
SphI 437 GCATGC GCATG^C
SspI 517 AATATT AAT^ATT
StyI 121 CCATGG C^C[AT][AT]GG
TfiI 785 GATTC G^A[AT]TC
TspRI 133 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 469 CCACGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 393 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
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Alw44I 137 GTGCAC G^TGCAC
305 GTGCAC
ApaLI 137 GTGCAC G^TGCAC
305 GTGCAC
BanII 125 GGGCTC G[GA]GC[CT]^C
622 GAGCTC
BsaJI 35 CCGTGG C^C[ACGT][ACGT]GG
121 CCATGG
BsiEI 32 CGGCCG CG[GA][CT]^CG
291 CGACCG
BsrI 820 ACTGG ACTGG(1/-1)
584 CCAGT
Cac8I 437 GCATGC GC[ACGT]^[ACGT]GC
485 GCAAGC
CfoI 78 GCGC GCG^C
371 GCGC
DraIII 131 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
470 CACGCTGTG
DsaI 35 CCGTGG C^C[GA][CT]GG
121 CCATGG
EaeI 32 CGGCCG [CT]^GGCC[GA]
895 TGGCCA
HhaI 78 GCGC GCG^C
371 GCGC
HinP1I 78 GCGC G^CGC
371 GCGC
HindIII 734 AAGCTT A^AGCTT
881 AAGCTT
MspA1I 23 CAGCTG C[AC]G^C[GT]G
910 CAGCTG
PleI 420 GACTC GAGTC(4/5)
569 GACTC
PvuII 23 CAGCTG CAG^CTG
910 CAGCTG
SmlI 182 CTCAAG C^T[CT][GA]AG
461 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AcsI AluI ApoI Asp700I
AspHI BbvCI BbvI BfaI BmyI
BseRI BsiHKAI BsiYI BslI BsmFI
Bsp1286I DdeI Fnu4HI HaeIII HinfI
ItaI MaeI MboII MnlI MseI
MwoI NlaIII NlaIV NspI SfaNI
TaqI Tru9I TseI Tsp509I TthHB8I
XmnI