Restriction map for
pFN21AB6414
(Linear) (Six-base) MAPSORT of: KIBB6414.seq Check: 1114 from: 1 to: 1005
KIBB6414.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 242 686 1005
Size: 230 12 444 319
Fragments arranged by size:
444 319 230 12
AfeI AGC'GCT
Cuts at: 0 372 1005
Size: 372 633
ApaLI G'TGCA_C
Cuts at: 0 137 305 1005
Size: 137 168 700
Fragments arranged by size:
700 168 137
ApoI r'AATT_y
Cuts at: 0 276 500 797 1005
Size: 276 224 297 208
Fragments arranged by size:
297 276 224 208
BanI G'GyrC_C
Cuts at: 0 117 1005
Size: 117 888
BanII G_rGCy'C
Cuts at: 0 129 626 1005
Size: 129 497 379
Fragments arranged by size:
497 379 129
BbvCI CC'TCA_GC
Cuts at: 0 17 602 908 1005
Size: 17 585 306 97
Fragments arranged by size:
585 306 97 17
BfrBI ATG'CAT
Cuts at: 0 346 1005
Size: 346 659
BglII A'GATC_T
Cuts at: 0 393 1005
Size: 393 612
BlpI GC'TnA_GC
Cuts at: 0 51 1005
Size: 51 954
Bme1580I G_kGCm'C
Cuts at: 0 120 141 309 1005
Size: 120 21 168 696
Fragments arranged by size:
696 168 120 21
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 66 1005
Size: 66 939
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 638 1005
Size: 638 367
Bpu10I CC'TnA_GC
Cuts at: 0 17 602 908 1005
Size: 17 585 306 97
Fragments arranged by size:
585 306 97 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 446 1005
Size: 167 279 559
Fragments arranged by size:
559 279 167
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 82 449 1005
Size: 55 27 367 556
Fragments arranged by size:
556 367 55 27
BseYI C'CCAG_C
Cuts at: 0 367 1005
Size: 367 638
BsiEI CG_ry'CG
Cuts at: 0 35 294 1005
Size: 35 259 711
Fragments arranged by size:
711 259 35
BsiHKAI G_wGCw'C
Cuts at: 0 141 309 626 1005
Size: 141 168 317 379
Fragments arranged by size:
379 317 168 141
Bsp1286I G_dGCh'C
Cuts at: 0 120 129 141 309 626 1005
Size: 120 9 12 168 317 379
Fragments arranged by size:
379 317 168 120 12 9
BstYI r'GATC_y
Cuts at: 0 393 1005
Size: 393 612
BtgI C'CryG_G
Cuts at: 0 35 121 1005
Size: 35 86 884
Fragments arranged by size:
884 86 35
DraIII CAC_nnn'GTG
Cuts at: 0 136 475 1005
Size: 136 339 530
Fragments arranged by size:
530 339 136
EaeI y'GGCC_r
Cuts at: 0 32 895 1005
Size: 32 863 110
Fragments arranged by size:
863 110 32
EagI C'GGCC_G
Cuts at: 0 32 1005
Size: 32 973
EarI CTCTTCn'nnn_
Cuts at: 0 548 1005
Size: 548 457
EcoICRI GAG'CTC
Cuts at: 0 624 1005
Size: 624 381
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 242 638 686 1005
Size: 230 12 396 48 319
Fragments arranged by size:
396 319 230 48 12
EcoRI G'AATT_C
Cuts at: 0 276 1005
Size: 276 729
EcoRV GAT'ATC
Cuts at: 0 540 1005
Size: 540 465
HaeII r_GCGC'y
Cuts at: 0 374 1005
Size: 374 631
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 529 554 561 586 1005
Size: 529 25 7 25 419
Fragments arranged by size:
529 419 25 25 7
HincII GTy'rAC
Cuts at: 0 323 1005
Size: 323 682
HindIII A'AGCT_T
Cuts at: 0 734 881 1005
Size: 734 147 124
Fragments arranged by size:
734 147 124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 1005
Size: 182 823
MscI TGG'CCA
Cuts at: 0 897 1005
Size: 897 108
MspA1I CmG'CkG
Cuts at: 0 25 912 1005
Size: 25 887 93
Fragments arranged by size:
887 93 25
NcoI C'CATG_G
Cuts at: 0 121 1005
Size: 121 884
NsiI A_TGCA'T
Cuts at: 0 348 1005
Size: 348 657
NspI r_CATG'y
Cuts at: 0 313 346 441 1005
Size: 313 33 95 564
Fragments arranged by size:
564 313 95 33
PflMI CCAn_nnn'nTGG
Cuts at: 0 475 1005
Size: 475 530
PvuII CAG'CTG
Cuts at: 0 25 912 1005
Size: 25 887 93
Fragments arranged by size:
887 93 25
SacI G_AGCT'C
Cuts at: 0 626 1005
Size: 626 379
ScaI AGT'ACT
Cuts at: 0 819 1005
Size: 819 186
SfcI C'TryA_G
Cuts at: 0 26 1005
Size: 26 979
SmlI C'TyrA_G
Cuts at: 0 182 461 1005
Size: 182 279 544
Fragments arranged by size:
544 279 182
SphI G_CATG'C
Cuts at: 0 441 1005
Size: 441 564
SspI AAT'ATT
Cuts at: 0 519 1005
Size: 519 486
StyI C'CwwG_G
Cuts at: 0 121 1005
Size: 121 884
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 308 1005
Size: 308 697
TatI w'GTAC_w
Cuts at: 0 817 1005
Size: 817 188
XmnI GAAnn'nnTTC
Cuts at: 0 215 503 557 863 1005
Size: 215 288 54 306 142
Fragments arranged by size:
306 288 215 142 54
Enzymes that do cut:
AcuI AfeI ApaLI ApoI BanI BanII BbvCI BfrBI
BglII BlpI Bme1580I BplI BpmI Bpu10I BpuEI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BstYI BtgI DraIII EaeI
EagI EarI EcoICRI Eco57MI EcoRI EcoRV HaeII Hin4I
HincII HindIII MmeI MscI MspA1I NcoI NsiI NspI
PflMI PvuII SacI ScaI SfcI SmlI SphI SspI
StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BcgI
BciVI BclI BglI BmgBI BmrI BmtI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BsgI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DrdI EciI EcoNI EcoO109I
FalI FseI FspI FspAI HpaI KasI KpnI MfeI
MluI MslI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI ZraI