(Circular) (Six-base) MAPSORT of: pFN21AF0316.seq Check: 603 from: 1 to: 6625 pFN21AF0316 6625 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4435 278 Size: 53 83 186 936 2899 2468 Fragments arranged by size: 2899 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3968 1937 Size: 2031 4594 Acc65I G'GTAC_C Cuts at: 3946 3946 Size: 6625 AclI AA'CG_TT Cuts at: 1563 4752 5125 1563 Size: 3189 373 3063 Fragments arranged by size: 3189 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3728 4688 6072 6340 1632 Size: 2096 960 1384 268 1917 Fragments arranged by size: 2096 1917 1384 960 268 AflII C'TTAA_G Cuts at: 820 1017 3278 3728 820 Size: 197 2261 450 3717 Fragments arranged by size: 3717 2261 450 197 AflIII A'CryG_T Cuts at: 1203 5524 6501 1203 Size: 4321 977 1327 Fragments arranged by size: 4321 1327 977 AgeI A'CCGG_T Cuts at: 5456 5456 Size: 6625 AhdI GACnn_n'nnGTC Cuts at: 5354 5354 Size: 6625 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6625 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2533 1184 Size: 1349 5276 AlwNI CAG_nnn'CTG Cuts at: 2057 2464 3026 3073 3103 3559 4368 5940 2057 Size: 407 562 47 30 456 809 1572 2742 Fragments arranged by size: 2742 1572 809 562 456 407 47 30 ApaLI G'TGCA_C Cuts at: 4682 5838 4682 Size: 1156 5469 ApoI r'AATT_y Cuts at: 1493 2119 2688 2695 3182 3321 3737 3934 4160 Size: 626 569 7 487 139 416 197 226 Cuts at: 4160 4312 6421 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 626 569 487 416 226 197 152 139 7 AseI AT'TA_AT Cuts at: 160 1032 3774 5179 160 Size: 872 2742 1405 1606 Fragments arranged by size: 2742 1606 1405 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6625 AvaI C'yCGr_G Cuts at: 1870 1943 3950 1870 Size: 73 2007 4545 Fragments arranged by size: 4545 2007 73 BamHI G'GATC_C Cuts at: 3955 4322 3955 Size: 367 6258 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3946 5401 618 Size: 325 217 627 2159 1455 1842 Fragments arranged by size: 2159 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2255 3944 1391 Size: 864 1689 4072 Fragments arranged by size: 4072 1689 864 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3139 928 Size: 971 1240 4414 Fragments arranged by size: 4414 1240 971 BbvCI CC'TCA_GC Cuts at: 2538 2538 Size: 6625 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4816 4850 1903 Size: 34 2879 34 3678 Fragments arranged by size: 3678 2879 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4517 5727 4517 Size: 1210 5415 BclI T'GATC_A Cuts at: 1548 1548 Size: 6625 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5236 136 Size: 107 122 71 631 605 3564 1525 Fragments arranged by size: 3564 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6620 6620 Size: 6625 BlpI GC'TnA_GC Cuts at: 3273 4042 3273 Size: 769 5856 Bme1580I G_kGCm'C Cuts at: 1790 2037 2301 3399 4686 5842 1790 Size: 247 264 1098 1287 1156 2573 Fragments arranged by size: 2573 1287 1156 1098 264 247 BmgBI CAC'GTC Cuts at: 1318 3112 1318 Size: 1794 4831 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4356 5314 442 Size: 416 53 83 399 24 2939 958 1753 Fragments arranged by size: 2939 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2495 3849 1056 Size: 1439 1354 3832 Fragments arranged by size: 3832 1439 1354 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2486 2530 2624 2669 3002 3080 3357 3413 Size: 1247 44 94 45 333 78 277 56 Cuts at: 3413 3519 5285 1239 Size: 106 1766 2579 Fragments arranged by size: 2579 1766 1247 333 277 106 94 78 56 45 44 Bpu10I CC'TnA_GC Cuts at: 2538 2538 Size: 6625 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3032 3588 3623 4750 5615 5913 3032 Size: 556 35 1127 865 298 3744 Fragments arranged by size: 3744 1127 865 556 298 35 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5288 882 Size: 617 3789 2219 Fragments arranged by size: 3789 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3920 4416 493 Size: 711 2716 496 2702 Fragments arranged by size: 2716 2702 711 496 BsaBI GATnn'nnATC Cuts at: 6619 6619 Size: 6625 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4432 4814 5449 275 Size: 53 83 186 936 2899 382 635 1451 Fragments arranged by size: 2899 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5058 5456 5730 5877 6288 5058 Size: 398 274 147 411 5395 Fragments arranged by size: 5395 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2597 2627 3063 3093 3216 Size: 30 165 30 1194 30 436 30 123 Cuts at: 3216 3246 1178 Size: 30 4557 Fragments arranged by size: 4557 1194 436 165 123 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6625 BseYI C'CCAG_C Cuts at: 2923 3006 3019 3066 5828 6324 2923 Size: 83 13 47 2762 496 3224 Fragments arranged by size: 3224 2762 496 83 47 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2282 2459 3050 1163 Size: 573 546 177 591 4738 Fragments arranged by size: 4738 591 573 546 177 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4075 4836 5864 1540 Size: 275 186 2074 761 1028 2301 Fragments arranged by size: 2301 2074 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2447 3159 3297 4686 4771 5842 2447 Size: 712 138 1389 85 1071 3230 Fragments arranged by size: 3230 1389 1071 712 138 85 BsmI GAATG_Cn' Cuts at: 2659 2992 4136 4229 2659 Size: 333 1144 93 5055 Fragments arranged by size: 5055 1144 333 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2255 2301 2447 3159 3297 3399 Size: 399 247 218 46 146 712 138 102 Cuts at: 3399 3944 4686 4771 5842 1391 Size: 545 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 712 545 399 247 218 146 138 102 85 46 BspEI T'CCGG_A Cuts at: 6288 6288 Size: 6625 BspHI T'CATG_A Cuts at: 2067 2211 4514 6244 2067 Size: 144 2303 1730 2448 Fragments arranged by size: 2448 2303 1730 144 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3980 844 Size: 3136 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3523 4512 1394 Size: 429 1700 989 3507 Fragments arranged by size: 3507 1700 989 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2242 3138 5120 5294 65 Size: 1183 994 896 1982 174 1396 Fragments arranged by size: 1982 1396 1183 994 896 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2760 5269 5456 1700 Size: 110 950 2509 187 2869 Fragments arranged by size: 2869 2509 950 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6625 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6625 BssSI C'ACGA_G Cuts at: 2386 4685 5697 2386 Size: 2299 1012 3314 Fragments arranged by size: 3314 2299 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6625 BstBI TT'CG_AA Cuts at: 5429 5429 Size: 6625 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6625 BstXI CCAn_nnnn'nTGG Cuts at: 2071 2071 Size: 6625 BstYI r'GATC_y Cuts at: 1972 2391 3831 3955 4322 4707 4724 6176 6262 Size: 419 1440 124 367 385 17 1452 86 Cuts at: 6262 6274 6620 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1440 419 385 367 346 124 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2160 2256 513 Size: 552 939 156 96 4882 Fragments arranged by size: 4882 939 552 156 96 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2370 487 Size: 683 56 1144 4742 Fragments arranged by size: 4742 1144 683 56 BtsI GCAGTG_nn' Cuts at: 2037 2997 3492 4141 4935 4955 2037 Size: 960 495 649 794 20 3707 Fragments arranged by size: 3707 960 794 649 495 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6625 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6590 DraI TTT'AAA Cuts at: 2787 3929 2787 Size: 1142 5483 DraIII CAC_nnn'GTG Cuts at: 2482 2482 Size: 6625 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5632 809 Size: 507 4316 1802 Fragments arranged by size: 4316 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2172 4072 4961 Size: 54 1425 50 275 18 342 1900 889 Cuts at: 4961 6590 8 Size: 1629 43 Fragments arranged by size: 1900 1629 1425 889 342 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4072 1537 Size: 275 2260 4090 Fragments arranged by size: 4090 2260 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2025 2097 3240 4555 1353 Size: 672 72 1143 1315 3423 Fragments arranged by size: 3423 1315 1143 672 72 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5212 5586 5732 387 Size: 1064 138 3623 374 146 1280 Fragments arranged by size: 3623 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2486 2530 2624 2669 3002 3080 3357 Size: 393 854 44 94 45 333 78 277 Cuts at: 3357 3413 3519 3728 4688 5285 6072 6340 1239 Size: 56 106 209 960 597 787 268 1524 Fragments arranged by size: 1524 960 854 787 597 393 333 277 268 209 106 94 78 56 45 44 EcoNI CCTnn'n_nnAGG Cuts at: 2894 3127 2894 Size: 233 6392 EcoO109I rG'GnC_Cy Cuts at: 2347 2461 3424 2347 Size: 114 963 5548 Fragments arranged by size: 5548 963 114 EcoRI G'AATT_C Cuts at: 2119 3182 3934 4312 2119 Size: 1063 752 378 4432 Fragments arranged by size: 4432 1063 752 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3244 3743 3244 Size: 499 6126 FspI TGC'GCA Cuts at: 5131 5131 Size: 6625 HaeII r_GCGC'y Cuts at: 1678 5772 1678 Size: 4094 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2703 2735 2886 2918 3333 Size: 32 135 32 1131 32 151 32 415 Cuts at: 3333 3365 3586 3618 3850 3882 3892 3924 5266 Size: 32 221 32 232 32 10 32 1342 Cuts at: 5266 5298 5340 5372 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1342 1131 415 232 221 151 135 42 32 32 32 32 32 32 32 32 32 32 10 HincII GTy'rAC Cuts at: 669 1657 1938 3969 4215 669 Size: 988 281 2031 246 3079 Fragments arranged by size: 3079 2031 988 281 246 HindIII A'AGCT_T Cuts at: 748 3194 3613 748 Size: 2446 419 3760 Fragments arranged by size: 3760 2446 419 HpaI GTT'AAC Cuts at: 4215 4215 Size: 6625 KpnI G_GTAC'C Cuts at: 3950 3950 Size: 6625 MfeI C'AATT_G Cuts at: 4224 4224 Size: 6625 MluI A'CGCG_T Cuts at: 6501 6501 Size: 6625 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5739 5923 1305 Size: 4434 184 2007 Fragments arranged by size: 4434 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2174 10 Size: 54 1425 343 342 4461 Fragments arranged by size: 4461 1425 343 342 54 MslI CAynn'nnrTG Cuts at: 518 1166 2069 2285 4583 4942 5101 518 Size: 648 903 216 2298 359 159 2042 Fragments arranged by size: 2298 2042 903 648 359 216 159 MspA1I CmG'CkG Cuts at: 1606 2110 2473 3023 3518 3542 4041 4718 5866 Size: 504 363 550 495 24 499 677 1148 Cuts at: 5866 6111 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 550 504 499 495 363 245 24 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5134 Fragments arranged by size: 5134 939 552 NdeI CA'TA_TG Cuts at: 387 2793 387 Size: 2406 4219 NheI G'CTAG_C Cuts at: 1052 2491 3845 1052 Size: 1439 1354 3832 Fragments arranged by size: 3832 1439 1354 NotI GC'GGCC_GC Cuts at: 4072 4072 Size: 6625 NspI r_CATG'y Cuts at: 1132 1229 2707 3119 3983 5528 1132 Size: 97 1478 412 864 1545 2229 Fragments arranged by size: 2229 1545 1478 864 412 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6625 PciI A'CATG_T Cuts at: 5524 5524 Size: 6625 PflMI CCAn_nnn'nTGG Cuts at: 1108 2326 2464 3061 1108 Size: 1218 138 597 4672 Fragments arranged by size: 4672 1218 597 138 PfoI T'CCnGG_A Cuts at: 3096 3334 3618 3096 Size: 238 284 6103 Fragments arranged by size: 6103 284 238 PmeI GTTT'AAAC Cuts at: 3929 3929 Size: 6625 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4694 4726 6207 6239 4694 Size: 32 1481 32 5080 Fragments arranged by size: 5080 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3424 3424 Size: 6625 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6625 PsiI TTA'TAA Cuts at: 4195 4195 Size: 6625 PstI C_TGCA'G Cuts at: 830 2032 3240 3444 3477 3977 830 Size: 1202 1208 204 33 500 3478 Fragments arranged by size: 3478 1208 1202 500 204 33 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6625 PvuII CAG'CTG Cuts at: 2110 2473 3542 2110 Size: 363 1069 5193 Fragments arranged by size: 5193 1069 363 SalI G'TCGA_C Cuts at: 1936 3967 1936 Size: 2031 4594 SapI GCTCTTCn'nnn_ Cuts at: 2097 2097 Size: 6625 SbfI CC_TGCA'GG Cuts at: 3977 3977 Size: 6625 ScaI AGT'ACT Cuts at: 4873 6585 4873 Size: 1712 4913 SexAI A'CCwGG_T Cuts at: 2041 2041 Size: 6625 SfcI C'TryA_G Cuts at: 826 1045 2028 3049 3236 3440 3473 3973 5108 Size: 219 983 1021 187 204 33 500 1135 Cuts at: 5108 5789 5980 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1021 983 681 500 219 204 191 187 33 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6625 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6625 SmaI CCC'GGG Cuts at: 1872 3952 1872 Size: 2080 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3011 3278 3603 3638 3728 4729 Size: 197 926 1068 267 325 35 90 1001 Cuts at: 4729 5630 5892 820 Size: 901 262 1553 Fragments arranged by size: 1553 1068 1001 926 901 325 267 262 197 90 35 SnaBI TAC'GTA Cuts at: 493 4416 493 Size: 3923 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6625 SphI G_CATG'C Cuts at: 1132 3119 3983 1132 Size: 1987 864 3774 Fragments arranged by size: 3774 1987 864 SspI AAT'ATT Cuts at: 5 52 2783 3263 4549 5 Size: 47 2731 480 1286 2081 Fragments arranged by size: 2731 2081 1286 480 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6625 StyI C'CwwG_G Cuts at: 513 1065 2004 4064 513 Size: 552 939 2060 3074 Fragments arranged by size: 3074 2060 939 552 SwaI ATTT'AAAT Cuts at: 2787 2787 Size: 6625 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 1997 2885 4652 4669 4822 5007 5452 Size: 1255 417 888 1767 17 153 185 445 Cuts at: 5452 325 Size: 1498 Fragments arranged by size: 1767 1498 1255 888 445 417 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4871 6583 96 Size: 274 80 33 51 1443 2894 1712 138 Fragments arranged by size: 2894 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2278 2310 3216 3248 6585 6617 2278 Size: 32 906 32 3337 32 2286 Fragments arranged by size: 3337 2286 906 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6625 XbaI T'CTAG_A Cuts at: 3961 3961 Size: 6625 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2086 2577 2086 Size: 491 6134 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6625 XmaI C'CCGG_G Cuts at: 1870 3950 1870 Size: 2080 4545 XmnI GAAnn'nnTTC Cuts at: 3669 3738 4754 3669 Size: 69 1016 5540 Fragments arranged by size: 5540 1016 69 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4433 276 Size: 53 83 186 936 2899 2468 Fragments arranged by size: 2899 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI