(Circular) (Six-base) MAPSORT of: pFN21AE0184.seq Check: 2880 from: 1 to: 5563 pFN21AE0184 5563 bp With 182 enzymes: SgfI * October 20, 2009 10:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3373 278 Size: 53 83 186 936 1837 2468 Fragments arranged by size: 2468 1837 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2906 1937 Size: 969 4594 Acc65I G'GTAC_C Cuts at: 2884 2884 Size: 5563 AclI AA'CG_TT Cuts at: 1563 3690 4063 1563 Size: 2127 373 3063 Fragments arranged by size: 3063 2127 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2623 2714 2864 3626 5010 5278 1632 Size: 991 91 150 762 1384 268 1917 Fragments arranged by size: 1917 1384 991 762 268 150 91 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5366 AflIII A'CryG_T Cuts at: 1203 2349 4462 5439 1203 Size: 1146 2113 977 1327 Fragments arranged by size: 2113 1327 1146 977 AgeI A'CCGG_T Cuts at: 4394 4394 Size: 5563 AhdI GACnn_n'nnGTC Cuts at: 4292 4292 Size: 5563 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5563 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2099 1184 Size: 915 4648 AlwNI CAG_nnn'CTG Cuts at: 2509 2526 3306 4878 2509 Size: 17 780 1572 3194 Fragments arranged by size: 3194 1572 780 17 ApaLI G'TGCA_C Cuts at: 2702 3620 4776 2702 Size: 918 1156 3489 Fragments arranged by size: 3489 1156 918 ApoI r'AATT_y Cuts at: 1493 2277 2450 2459 2872 3098 3250 5359 1493 Size: 784 173 9 413 226 152 2109 1697 Fragments arranged by size: 2109 1697 784 413 226 173 152 9 AseI AT'TA_AT Cuts at: 160 1032 4117 160 Size: 872 3085 1606 Fragments arranged by size: 3085 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5563 AvaI C'yCGr_G Cuts at: 1870 1943 2124 2888 1870 Size: 73 181 764 4545 Fragments arranged by size: 4545 764 181 73 BamHI G'GATC_C Cuts at: 2893 3260 2893 Size: 367 5196 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2884 4339 618 Size: 325 217 627 1097 1455 1842 Fragments arranged by size: 1842 1455 1097 627 325 217 BanII G_rGCy'C Cuts at: 1391 2626 2882 1391 Size: 1235 256 4072 Fragments arranged by size: 4072 1235 256 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2136 2748 2852 928 Size: 971 237 612 104 3639 Fragments arranged by size: 3639 971 612 237 104 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3754 3788 1903 Size: 34 1817 34 3678 Fragments arranged by size: 3678 1817 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3455 4665 3455 Size: 1210 4353 BclI T'GATC_A Cuts at: 1548 1548 Size: 5563 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4174 136 Size: 107 122 71 631 605 337 2165 1525 Fragments arranged by size: 2165 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2120 5558 2120 Size: 3438 2125 BlpI GC'TnA_GC Cuts at: 2980 2980 Size: 5563 Bme1580I G_kGCm'C Cuts at: 1790 2706 3624 4780 1790 Size: 916 918 1156 2573 Fragments arranged by size: 2573 1156 918 916 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5563 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2629 3294 4252 Size: 416 53 83 399 24 1212 665 958 Cuts at: 4252 442 Size: 1753 Fragments arranged by size: 1753 1212 958 665 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5563 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2407 2476 2843 4223 1239 Size: 1168 69 367 1380 2579 Fragments arranged by size: 2579 1380 1168 367 69 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3688 4553 4851 3688 Size: 865 298 4400 Fragments arranged by size: 4400 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4226 882 Size: 617 2727 2219 Fragments arranged by size: 2727 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2352 3354 493 Size: 711 1148 1002 2702 Fragments arranged by size: 2702 1148 1002 711 BsaBI GATnn'nnATC Cuts at: 5557 5557 Size: 5563 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3370 3752 4387 275 Size: 53 83 186 936 1837 382 635 1451 Fragments arranged by size: 1837 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2094 2157 2251 2549 3996 4394 4668 4815 5226 Size: 63 94 298 1447 398 274 147 411 Cuts at: 5226 2094 Size: 2431 Fragments arranged by size: 2431 1447 411 398 298 274 147 94 63 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5338 Fragments arranged by size: 5338 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5563 BseYI C'CCAG_C Cuts at: 2775 4766 5262 2775 Size: 1991 496 3076 Fragments arranged by size: 3076 1991 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4990 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3013 3774 4802 1540 Size: 275 186 1012 761 1028 2301 Fragments arranged by size: 2301 1028 1012 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2706 3624 3709 4780 2706 Size: 918 85 1071 3489 Fragments arranged by size: 3489 1071 918 85 BsmI GAATG_Cn' Cuts at: 2261 2758 3074 3167 2261 Size: 497 316 93 4657 Fragments arranged by size: 4657 497 316 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2626 2706 2882 3624 3709 4780 1391 Size: 399 836 80 176 742 85 1071 2174 Fragments arranged by size: 2174 1071 836 742 399 176 85 80 BspEI T'CCGG_A Cuts at: 2094 2157 2549 5226 2094 Size: 63 392 2677 2431 Fragments arranged by size: 2677 2431 392 63 BspHI T'CATG_A Cuts at: 2424 2832 3452 5182 2424 Size: 408 620 1730 2805 Fragments arranged by size: 2805 1730 620 408 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2918 844 Size: 2074 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2036 3450 1394 Size: 429 213 1414 3507 Fragments arranged by size: 3507 1414 429 213 BsrDI GCAATG_nn' Cuts at: 65 1248 4058 4232 65 Size: 1183 2810 174 1396 Fragments arranged by size: 2810 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2045 4207 4394 1700 Size: 110 235 2162 187 2869 Fragments arranged by size: 2869 2162 235 187 110 BsrGI T'GTAC_A Cuts at: 96 2694 96 Size: 2598 2965 BssHII G'CGCG_C Cuts at: 1925 2023 1925 Size: 98 5465 BssSI C'ACGA_G Cuts at: 2625 3623 4635 2625 Size: 998 1012 3553 Fragments arranged by size: 3553 1012 998 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5563 BstBI TT'CG_AA Cuts at: 4367 4367 Size: 5563 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5563 BstYI r'GATC_y Cuts at: 1972 2120 2546 2725 2893 3260 3645 3662 5114 Size: 148 426 179 168 367 385 17 1452 Cuts at: 5114 5200 5212 5558 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 426 385 367 346 179 168 148 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2029 513 Size: 552 939 25 4047 Fragments arranged by size: 4047 939 552 25 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4824 Fragments arranged by size: 4824 683 56 BtsI GCAGTG_nn' Cuts at: 3079 3873 3893 3079 Size: 794 20 4749 Fragments arranged by size: 4749 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5563 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5528 DraI TTT'AAA Cuts at: 2867 2867 Size: 5563 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2375 4570 809 Size: 507 1059 2195 1802 Fragments arranged by size: 2195 1802 1059 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3010 3899 5528 Size: 54 1425 50 275 18 1180 889 1629 Cuts at: 5528 8 Size: 43 Fragments arranged by size: 1629 1425 1180 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3010 1537 Size: 275 1198 4090 Fragments arranged by size: 4090 1198 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3493 1353 Size: 2140 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4150 4524 4670 387 Size: 1064 138 2561 374 146 1280 Fragments arranged by size: 2561 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2407 2476 2623 2714 2843 2864 3626 Size: 393 775 69 147 91 129 21 762 Cuts at: 3626 4223 5010 5278 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 775 762 597 393 268 147 129 91 69 21 EcoRI G'AATT_C Cuts at: 2872 3250 2872 Size: 378 5185 FspI TGC'GCA Cuts at: 4069 4069 Size: 5563 HaeII r_GCGC'y Cuts at: 1678 4710 1678 Size: 3032 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2171 2203 4204 4236 4278 Size: 32 135 32 599 32 2001 32 42 Cuts at: 4278 4310 1373 Size: 32 2626 Fragments arranged by size: 2626 2001 599 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2907 3153 669 Size: 988 281 969 246 3079 Fragments arranged by size: 3079 988 969 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5563 HpaI GTT'AAC Cuts at: 3153 3153 Size: 5563 KpnI G_GTAC'C Cuts at: 2888 2888 Size: 5563 MfeI C'AATT_G Cuts at: 3162 3162 Size: 5563 MluI A'CGCG_T Cuts at: 5439 5439 Size: 5563 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2493 2611 2644 4677 4861 1305 Size: 1188 118 33 2033 184 2007 Fragments arranged by size: 2033 2007 1188 184 118 33 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3741 Fragments arranged by size: 3741 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2769 3521 3880 4039 518 Size: 648 1603 752 359 159 2042 Fragments arranged by size: 2042 1603 752 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2031 2062 2136 2692 2979 3656 4804 5049 Size: 425 31 74 556 287 677 1148 245 Cuts at: 5049 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 556 425 287 245 74 31 NaeI GCC'GGC Cuts at: 2047 2047 Size: 5563 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4072 Fragments arranged by size: 4072 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5563 NgoMIV G'CCGG_C Cuts at: 2045 2045 Size: 5563 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5563 NotI GC'GGCC_GC Cuts at: 3010 3010 Size: 5563 NspI r_CATG'y Cuts at: 1132 1229 2485 2921 4466 1132 Size: 97 1256 436 1545 2229 Fragments arranged by size: 2229 1545 1256 436 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5563 PciI A'CATG_T Cuts at: 4462 4462 Size: 5563 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5563 PfoI T'CCnGG_A Cuts at: 2492 2492 Size: 5563 PmeI GTTT'AAAC Cuts at: 2867 2867 Size: 5563 PmlI CAC'GTG Cuts at: 2352 2352 Size: 5563 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3632 3664 5145 5177 3632 Size: 32 1481 32 4018 Fragments arranged by size: 4018 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5563 PsiI TTA'TAA Cuts at: 3133 3133 Size: 5563 PstI C_TGCA'G Cuts at: 830 2061 2078 2915 830 Size: 1231 17 837 3478 Fragments arranged by size: 3478 1231 837 17 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5563 PvuII CAG'CTG Cuts at: 2136 2692 2136 Size: 556 5007 SacII CC_GC'GG Cuts at: 2032 2032 Size: 5563 SalI G'TCGA_C Cuts at: 1936 2905 1936 Size: 969 4594 SbfI CC_TGCA'GG Cuts at: 2915 2915 Size: 5563 ScaI AGT'ACT Cuts at: 3811 5523 3811 Size: 1712 3851 SfcI C'TryA_G Cuts at: 826 1045 2057 2074 2911 4046 4727 4918 826 Size: 219 1012 17 837 1135 681 191 1471 Fragments arranged by size: 1471 1135 1012 837 681 219 191 17 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5563 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5563 SmaI CCC'GGG Cuts at: 1872 2890 1872 Size: 1018 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2124 3667 4568 4830 820 Size: 197 926 181 1543 901 262 1553 Fragments arranged by size: 1553 1543 926 901 262 197 181 SnaBI TAC'GTA Cuts at: 493 3354 493 Size: 2861 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5563 SphI G_CATG'C Cuts at: 1132 2921 1132 Size: 1789 3774 SspI AAT'ATT Cuts at: 5 52 3487 5 Size: 47 3435 2081 Fragments arranged by size: 3435 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5563 StyI C'CwwG_G Cuts at: 513 1065 2004 3002 513 Size: 552 939 998 3074 Fragments arranged by size: 3074 998 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3590 3607 3760 3945 4390 325 Size: 1255 2010 17 153 185 445 1498 Fragments arranged by size: 2010 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2327 2694 3809 Size: 274 80 33 51 1443 350 367 1115 Cuts at: 3809 5521 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1115 367 350 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5523 5555 5523 Size: 32 5531 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5563 XbaI T'CTAG_A Cuts at: 2899 2899 Size: 5563 XhoI C'TCGA_G Cuts at: 1943 2124 1943 Size: 181 5382 XmaI C'CCGG_G Cuts at: 1870 2888 1870 Size: 1018 4545 XmnI GAAnn'nnTTC Cuts at: 3692 3692 Size: 5563 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3371 276 Size: 53 83 186 936 1837 2468 Fragments arranged by size: 2468 1837 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI