################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Aor51HI ApaI AscI Asp718I AspEI AviII AvrII BbeI BbvCI BcgI BclI BfrI BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BsrBI BsrFI BssHII Bst1107I BstBI BstUI BstZ17I CelII Cfr10I ClaI CpoI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoT22I FauI FbaI FseI FspI HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MamI MluI MroI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmeI PpuMI PshAI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Alw44I 2820 GTGCAC G^TGCAC ApaLI 2820 GTGCAC G^TGCAC AseI 2587 ATTAAT AT^TAAT AsnI 2587 ATTAAT AT^TAAT BamHI 2123 GGATCC G^GATCC BanI 1771 GGTGCC G^G[CT][GA]CC BanII 1588 GAGCCC G[GA]GC[CT]^C BbrPI 2321 CACGTG CAC^GTG BciVI 1731 GTATCC GTATCC(6/5) BcnI 1176 CCGGG CC^[GC]GG BglII 3008 AGATCT A^GATCT BsaAI 2321 CACGTG [CT]AC^GT[GA] BsaWI 2800 ACCGGA [AT]^CCGG[AT] BseRI 1914 CTCCTC GAGGAG(10/8) BsmFI 1955 GGGAC GGGAC(10/14) BspHI 2684 TCATGA T^CATGA BspMI 2459 ACCTGC ACCTGC(4/8) BssSI 2303 CACGAG CACGAG(-5/-1) BstEII 1939 GGTGACC G^GT[ACGT]ACC BstPI 1939 GGTGACC G^GT[ACGT]ACC BstXI 2037 CCATATTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 2207 GCGC GCG^C DraI 1434 TTTAAA TTT^AAA DsaI 1775 CCATGG C^C[GA][CT]GG EcoO65I 1939 GGTGACC G^GT[ACGT]ACC EcoRI 2690 GAATTC G^AATTC HaeII 2206 GGCGCT [GA]GCGC^[CT] HhaI 2207 GCGC GCG^C HinP1I 2207 GCGC G^CGC HindIII 1670 AAGCTT A^AGCTT MslI 1858 CATAGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NciI 1176 CCGGG CC^[GC]GG NcoI 1775 CCATGG C^CATGG PmaCI 2321 CACGTG CAC^GTG PmlI 2321 CACGTG CAC^GTG PshBI 2587 ATTAAT AT^TAAT RcaI 2684 TCATGA T^CATGA SspI 2946 AATATT AAT^ATT StuI 497 AGGCCT AGG^CCT TaqI 2455 TCGA T^CGA TthHB8I 2455 TCGA T^CGA XbaI 858 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AspHI 1691 GTGCTC G[AT]GC[AT]^C 2820 GTGCAC AspI 2635 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2662 GACATAGTC AvaI 8 CCCGAG C^[CT]CG[GA]G 2483 CTCGGG AvaII 217 GGACC G^G[AT]CC 648 GGACC BalI 2180 TGGCCA TGG^CCA 2972 TGGCCA BbsI 1853 GTCTTC GAAGAC(2/6) 2134 GTCTTC BpuAI 1853 GTCTTC GAAGAC(2/6) 2134 GTCTTC BsiHKAI 1691 GTGCTC G[AT]GC[AT]^C 2820 GTGCAC BsmAI 1037 GAGAC GTCTC(1/5) 69 GTCTC BsoBI 8 CCCGAG C^[CT]CG[GA]G 2483 CTCGGG BspLU11I 1727 ACATGT A^CATGT 2019 ACATGT EaeI 2180 TGGCCA [CT]^GGCC[GA] 2972 TGGCCA EcoNI 682 CCTAACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2163 CCTCTCTGAGG EcoRV 1327 GATATC GAT^ATC 2254 GATATC HapII 1176 CCGG C^CGG 2801 CCGG HpaII 1176 CCGG C^CGG 2801 CCGG MaeII 2010 ACGT A^CGT 2322 ACGT MfeI 557 CAATTG C^AATTG 2630 CAATTG MluNI 2180 TGGCCA TGG^CCA 2972 TGGCCA MscI 2180 TGGCCA TGG^CCA 2972 TGGCCA MspI 1176 CCGG C^CGG 2801 CCGG MunI 557 CAATTG C^AATTG 2630 CAATTG PflFI 2635 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2662 GACATAGTC SfaNI 2107 GATGC GCATC(5/9) 2851 GATGC SmlI 1079 CTCAAG C^T[CT][GA]AG 1198 CTTGAG SphI 782 GCATGC GCATG^C 2351 GCATGC Tth111I 2635 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2662 GACATAGTC ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI Asp700I BbvI BfaI BglI BmyI BpmI BsaJI BsgI BsiYI BslI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII EarI Eco81I EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScaI ScrFI SfcI SspBI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII XmnI