(Circular) (Six-base) MAPSORT of: pFN21AB3014.seq Check: 6807 from: 1 to: 7735 pFN21AB3014 7735 bp With 182 enzymes: SgfI * August 16, 2010 14:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5545 278 Size: 53 83 186 936 4009 2468 Fragments arranged by size: 4009 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5078 1937 Size: 3141 4594 Acc65I G'GTAC_C Cuts at: 5056 5056 Size: 7735 AclI AA'CG_TT Cuts at: 1563 5862 6235 1563 Size: 4299 373 3063 Fragments arranged by size: 4299 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2415 2753 3669 4335 4866 4952 5798 7182 Size: 783 338 916 666 531 86 846 1384 Cuts at: 7182 7450 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 916 846 783 666 531 338 268 86 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7538 AflIII A'CryG_T Cuts at: 1203 3732 4024 4327 6634 7611 1203 Size: 2529 292 303 2307 977 1327 Fragments arranged by size: 2529 2307 1327 977 303 292 AgeI A'CCGG_T Cuts at: 6566 6566 Size: 7735 AhdI GACnn_n'nnGTC Cuts at: 6464 6464 Size: 7735 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7735 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2993 1184 Size: 1809 5926 AlwNI CAG_nnn'CTG Cuts at: 2120 3119 3932 4252 4547 4820 5478 7050 2120 Size: 999 813 320 295 273 658 1572 2805 Fragments arranged by size: 2805 1572 999 813 658 320 295 273 ApaLI G'TGCA_C Cuts at: 4825 5792 6948 4825 Size: 967 1156 5612 Fragments arranged by size: 5612 1156 967 ApoI r'AATT_y Cuts at: 1493 2207 3363 4622 4695 5044 5270 5422 7531 Size: 714 1156 1259 73 349 226 152 2109 Cuts at: 7531 1493 Size: 1697 Fragments arranged by size: 2109 1697 1259 1156 714 349 226 152 73 AseI AT'TA_AT Cuts at: 160 1032 4593 6289 160 Size: 872 3561 1696 1606 Fragments arranged by size: 3561 1696 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7735 AvaI C'yCGr_G Cuts at: 1870 1943 2013 4488 5060 1870 Size: 73 70 2475 572 4545 Fragments arranged by size: 4545 2475 572 73 70 BamHI G'GATC_C Cuts at: 4128 5065 5432 4128 Size: 937 367 6431 Fragments arranged by size: 6431 937 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3776 5056 6511 618 Size: 325 217 627 1989 1280 1455 1842 Fragments arranged by size: 1989 1842 1455 1280 627 325 217 BanII G_rGCy'C Cuts at: 1391 3597 5054 1391 Size: 2206 1457 4072 Fragments arranged by size: 4072 2206 1457 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3851 4132 928 Size: 971 1952 281 4531 Fragments arranged by size: 4531 1952 971 281 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5926 5960 1903 Size: 34 3989 34 3678 Fragments arranged by size: 3989 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3747 5627 6837 3747 Size: 1880 1210 4645 Fragments arranged by size: 4645 1880 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7735 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2546 2986 4433 Size: 107 122 71 631 605 874 440 1447 Cuts at: 4433 6346 136 Size: 1913 1525 Fragments arranged by size: 1913 1525 1447 874 631 605 440 122 107 71 BglII A'GATC_T Cuts at: 5013 7730 5013 Size: 2717 5018 BlpI GC'TnA_GC Cuts at: 5152 5152 Size: 7735 Bme1580I G_kGCm'C Cuts at: 1790 4829 5796 6952 1790 Size: 3039 967 1156 2573 Fragments arranged by size: 3039 2573 1156 967 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7735 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4830 5466 6424 Size: 416 53 83 399 24 3413 636 958 Cuts at: 6424 442 Size: 1753 Fragments arranged by size: 3413 1753 958 636 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7735 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2574 2690 3060 3119 3275 3345 3765 3896 Size: 1335 116 370 59 156 70 420 131 Cuts at: 3896 3909 4144 6395 1239 Size: 13 235 2251 2579 Fragments arranged by size: 2579 2251 1335 420 370 235 156 131 116 70 59 13 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3069 3224 5860 6725 7023 3069 Size: 155 2636 865 298 3781 Fragments arranged by size: 3781 2636 865 298 155 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6398 882 Size: 617 4899 2219 Fragments arranged by size: 4899 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4328 5526 493 Size: 711 3124 1198 2702 Fragments arranged by size: 3124 2702 1198 711 BsaBI GATnn'nnATC Cuts at: 7729 7729 Size: 7735 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5542 5924 6559 275 Size: 53 83 186 936 4009 382 635 1451 Fragments arranged by size: 4009 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4805 6168 6566 6840 6987 7398 4805 Size: 1363 398 274 147 411 5142 Fragments arranged by size: 5142 1363 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2580 2610 4800 4830 1178 Size: 30 165 30 1177 30 2190 30 4083 Fragments arranged by size: 4083 2190 1177 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3910 1186 Size: 2724 5011 BseYI C'CCAG_C Cuts at: 2243 2644 3513 3761 4547 6938 7434 2243 Size: 401 869 248 786 2391 496 2544 Fragments arranged by size: 2544 2391 869 786 496 401 248 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2442 2953 4491 1163 Size: 573 706 511 1538 4407 Fragments arranged by size: 4407 1538 706 573 511 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5185 5946 6974 1540 Size: 275 186 3184 761 1028 2301 Fragments arranged by size: 3184 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3700 4829 5796 5881 6952 3700 Size: 1129 967 85 1071 4483 Fragments arranged by size: 4483 1129 1071 967 85 BsmI GAATG_Cn' Cuts at: 5246 5339 5246 Size: 93 7642 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3597 3700 4829 5054 5796 5881 6952 Size: 399 1807 103 1129 225 742 85 1071 Cuts at: 6952 1391 Size: 2174 Fragments arranged by size: 2174 1807 1129 1071 742 399 225 103 85 BspEI T'CCGG_A Cuts at: 7398 7398 Size: 7735 BspHI T'CATG_A Cuts at: 4689 5624 7354 4689 Size: 935 1730 5070 Fragments arranged by size: 5070 1730 935 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4473 5090 844 Size: 3629 617 3489 Fragments arranged by size: 3629 3489 617 BsrBI CCG'CTC Cuts at: 1394 1823 5622 1394 Size: 429 3799 3507 Fragments arranged by size: 3799 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2285 3564 3874 6230 6404 65 Size: 1183 1037 1279 310 2356 174 1396 Fragments arranged by size: 2356 1396 1279 1183 1037 310 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6379 6566 1700 Size: 110 4569 187 2869 Fragments arranged by size: 4569 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2515 4831 4990 96 Size: 2419 2316 159 2841 Fragments arranged by size: 2841 2419 2316 159 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7735 BssSI C'ACGA_G Cuts at: 4308 5795 6807 4308 Size: 1487 1012 5236 Fragments arranged by size: 5236 1487 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2120 2124 3119 794 Size: 1326 4 995 5410 Fragments arranged by size: 5410 1326 995 4 BstBI TT'CG_AA Cuts at: 6539 6539 Size: 7735 BstEII G'GTnAC_C Cuts at: 1184 3944 1184 Size: 2760 4975 BstXI CCAn_nnnn'nTGG Cuts at: 4049 4049 Size: 7735 BstYI r'GATC_y Cuts at: 1972 3460 4128 5013 5065 5432 5817 5834 7286 Size: 1488 668 885 52 367 385 17 1452 Cuts at: 7286 7372 7384 7730 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1488 1452 885 668 385 367 346 86 52 17 12 Bsu36I CC'TnA_GG Cuts at: 2261 2724 3078 2261 Size: 463 354 6918 Fragments arranged by size: 6918 463 354 BtgI C'CryG_G Cuts at: 513 1065 2004 3780 513 Size: 552 939 1776 4468 Fragments arranged by size: 4468 1776 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6996 Fragments arranged by size: 6996 683 56 BtsI GCAGTG_nn' Cuts at: 2038 2118 2169 3117 4303 5251 6045 6065 2038 Size: 80 51 948 1186 948 794 20 3708 Fragments arranged by size: 3708 1186 948 948 794 80 51 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7735 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7700 DraI TTT'AAA Cuts at: 3441 5039 3441 Size: 1598 6137 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6742 809 Size: 507 5426 1802 Fragments arranged by size: 5426 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4185 4977 5182 Size: 54 1425 50 275 18 2355 792 205 Cuts at: 5182 6071 7700 8 Size: 889 1629 43 Fragments arranged by size: 2355 1629 1425 889 792 275 205 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5182 1537 Size: 275 3370 4090 Fragments arranged by size: 4090 3370 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2493 2586 3605 4173 4647 4889 5665 1353 Size: 1140 93 1019 568 474 242 776 3423 Fragments arranged by size: 3423 1140 1019 776 568 474 242 93 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6322 6696 6842 387 Size: 1064 138 4733 374 146 1280 Fragments arranged by size: 4733 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2415 2574 2690 2753 3060 3119 3275 Size: 393 783 159 116 63 307 59 156 Cuts at: 3275 3345 3669 3765 3896 3909 4144 4335 4866 Size: 70 324 96 131 13 235 191 531 Cuts at: 4866 4952 5798 6395 7182 7450 1239 Size: 86 846 597 787 268 1524 Fragments arranged by size: 1524 846 787 783 597 531 393 324 307 268 235 191 159 156 131 116 96 86 70 63 59 13 EcoNI CCTnn'n_nnAGG Cuts at: 2691 4172 2691 Size: 1481 6254 EcoRI G'AATT_C Cuts at: 4695 5044 5422 4695 Size: 349 378 7008 Fragments arranged by size: 7008 378 349 EcoRV GAT'ATC Cuts at: 3334 4261 3334 Size: 927 6808 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2597 3428 3482 2597 Size: 831 54 6850 Fragments arranged by size: 6850 831 54 FspI TGC'GCA Cuts at: 6241 6241 Size: 7735 HaeII r_GCGC'y Cuts at: 1678 4215 6882 1678 Size: 2537 2667 2531 Fragments arranged by size: 2667 2537 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2343 2375 3318 3350 3684 Size: 32 135 32 771 32 943 32 334 Cuts at: 3684 3716 4422 4454 6376 6408 6450 6482 1373 Size: 32 706 32 1922 32 42 32 2626 Fragments arranged by size: 2626 1922 943 771 706 334 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5079 5325 669 Size: 988 281 3141 246 3079 Fragments arranged by size: 3141 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 3675 748 Size: 2927 4808 HpaI GTT'AAC Cuts at: 5325 5325 Size: 7735 KpnI G_GTAC'C Cuts at: 5060 5060 Size: 7735 MfeI C'AATT_G Cuts at: 2562 4635 5334 2562 Size: 2073 699 4963 Fragments arranged by size: 4963 2073 699 MluI A'CGCG_T Cuts at: 7611 7611 Size: 7735 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2200 3472 3820 4554 6849 7033 1305 Size: 895 1272 348 734 2295 184 2007 Fragments arranged by size: 2295 2007 1272 895 734 348 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4187 4979 10 Size: 54 1425 343 2355 792 2766 Fragments arranged by size: 2766 2355 1425 792 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3867 5693 6052 6211 518 Size: 648 2701 1826 359 159 2042 Fragments arranged by size: 2701 2042 1826 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2465 2552 3341 3449 4972 5151 5828 6976 Size: 859 87 789 108 1523 179 677 1148 Cuts at: 6976 7221 1606 Size: 245 2120 Fragments arranged by size: 2120 1523 1148 859 789 677 245 179 108 87 NcoI C'CATG_G Cuts at: 513 1065 2004 3780 513 Size: 552 939 1776 4468 Fragments arranged by size: 4468 1776 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7735 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7735 NotI GC'GGCC_GC Cuts at: 5182 5182 Size: 7735 NspI r_CATG'y Cuts at: 1132 1229 2791 3736 4028 4360 5093 6638 1132 Size: 97 1562 945 292 332 733 1545 2229 Fragments arranged by size: 2229 1562 1545 945 733 332 292 97 PasI CC'CwG_GG Cuts at: 1348 4134 1348 Size: 2786 4949 PciI A'CATG_T Cuts at: 3732 4024 6634 3732 Size: 292 2610 4833 Fragments arranged by size: 4833 2610 292 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7735 PfoI T'CCnGG_A Cuts at: 3037 3180 3037 Size: 143 7592 PmeI GTTT'AAAC Cuts at: 5039 5039 Size: 7735 PmlI CAC'GTG Cuts at: 4328 4328 Size: 7735 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5804 5836 7317 7349 5804 Size: 32 1481 32 6190 Fragments arranged by size: 6190 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7735 PsiI TTA'TAA Cuts at: 2024 5305 2024 Size: 3281 4454 PstI C_TGCA'G Cuts at: 830 2272 2408 4963 5087 830 Size: 1442 136 2555 124 3478 Fragments arranged by size: 3478 2555 1442 136 124 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7735 PvuII CAG'CTG Cuts at: 2465 2552 3341 3449 4972 2465 Size: 87 789 108 1523 5228 Fragments arranged by size: 5228 1523 789 108 87 SalI G'TCGA_C Cuts at: 1936 5077 1936 Size: 3141 4594 SbfI CC_TGCA'GG Cuts at: 5087 5087 Size: 7735 ScaI AGT'ACT Cuts at: 2019 3917 4486 5983 7695 2019 Size: 1898 569 1497 1712 2059 Fragments arranged by size: 2059 1898 1712 1497 569 SfcI C'TryA_G Cuts at: 826 1045 2268 2404 3988 4959 5083 6218 6899 Size: 219 1223 136 1584 971 124 1135 681 Cuts at: 6899 7090 826 Size: 191 1471 Fragments arranged by size: 1584 1471 1223 1135 971 681 219 191 136 124 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7735 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7735 SmaI CCC'GGG Cuts at: 1872 5062 1872 Size: 3190 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3084 3203 5839 6740 7002 820 Size: 197 926 1141 119 2636 901 262 1553 Fragments arranged by size: 2636 1553 1141 926 901 262 197 119 SnaBI TAC'GTA Cuts at: 493 5526 493 Size: 5033 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7735 SphI G_CATG'C Cuts at: 1132 2791 4360 5093 1132 Size: 1659 1569 733 3774 Fragments arranged by size: 3774 1659 1569 733 SspI AAT'ATT Cuts at: 5 52 4953 5659 5 Size: 47 4901 706 2081 Fragments arranged by size: 4901 2081 706 47 StuI AGG'CCT Cuts at: 1517 2504 1517 Size: 987 6748 StyI C'CwwG_G Cuts at: 513 1065 2004 3051 3703 3780 5174 513 Size: 552 939 1047 652 77 1394 3074 Fragments arranged by size: 3074 1394 1047 939 652 552 77 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4276 4764 4812 5762 5779 5932 6117 Size: 1255 2696 488 48 950 17 153 185 Cuts at: 6117 6562 325 Size: 445 1498 Fragments arranged by size: 2696 1498 1255 950 488 445 185 153 48 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2017 2515 3915 Size: 274 80 33 51 1443 40 498 1400 Cuts at: 3915 4484 4831 4990 5981 7693 96 Size: 569 347 159 991 1712 138 Fragments arranged by size: 1712 1443 1400 991 569 498 347 274 159 138 80 51 40 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2460 2492 2580 2612 3087 3119 7695 7727 2460 Size: 32 88 32 475 32 4576 32 2468 Fragments arranged by size: 4576 2468 475 88 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4643 4670 1316 Size: 3327 27 4381 Fragments arranged by size: 4381 3327 27 XbaI T'CTAG_A Cuts at: 2863 5071 2863 Size: 2208 5527 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2341 2370 4049 4724 2341 Size: 29 1679 675 5352 Fragments arranged by size: 5352 1679 675 29 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7735 XmaI C'CCGG_G Cuts at: 1870 5060 1870 Size: 3190 4545 XmnI GAAnn'nnTTC Cuts at: 2381 2577 2940 3847 4699 4884 5864 2381 Size: 196 363 907 852 185 980 4252 Fragments arranged by size: 4252 980 907 852 363 196 185 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5543 276 Size: 53 83 186 936 4009 2468 Fragments arranged by size: 4009 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI