(Linear) (Six-base) MAPSORT of: KIBB3014.seq Check: 97 from: 1 to: 3030 KIBB3014.orf With 182 enzymes: SgfI * July 15, 2010 18:29 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 410 748 1664 2330 2861 2947 3030 Size: 410 338 916 666 531 86 83 Fragments arranged by size: 916 666 531 410 338 86 83 AflIII A'CryG_T Cuts at: 0 1727 2019 2322 3030 Size: 1727 292 303 708 Fragments arranged by size: 1727 708 303 292 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 988 3030 Size: 988 2042 AlwNI CAG_nnn'CTG Cuts at: 0 115 1114 1927 2247 2542 2815 3030 Size: 115 999 813 320 295 273 215 Fragments arranged by size: 999 813 320 295 273 215 115 ApaLI G'TGCA_C Cuts at: 0 2820 3030 Size: 2820 210 ApoI r'AATT_y Cuts at: 0 202 1358 2617 2690 3030 Size: 202 1156 1259 73 340 Fragments arranged by size: 1259 1156 340 202 73 AseI AT'TA_AT Cuts at: 0 2588 3030 Size: 2588 442 AvaI C'yCGr_G Cuts at: 0 8 2483 3030 Size: 8 2475 547 Fragments arranged by size: 2475 547 8 BamHI G'GATC_C Cuts at: 0 2123 3030 Size: 2123 907 BanI G'GyrC_C Cuts at: 0 1771 3030 Size: 1771 1259 BanII G_rGCy'C Cuts at: 0 1592 3030 Size: 1592 1438 BbsI GAAGACnn'nnnn_ Cuts at: 0 1846 2127 3030 Size: 1846 281 903 Fragments arranged by size: 1846 903 281 BciVI GTATCCnnnnn_n' Cuts at: 0 1742 3030 Size: 1742 1288 BglI GCCn_nnn'nGGC Cuts at: 0 541 981 2428 3030 Size: 541 440 1447 602 Fragments arranged by size: 1447 602 541 440 BglII A'GATC_T Cuts at: 0 3008 3030 Size: 3008 22 Bme1580I G_kGCm'C Cuts at: 0 2824 3030 Size: 2824 206 BmrI ACTGGGnnnn_n' Cuts at: 0 2825 3030 Size: 2825 205 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 569 685 1055 1114 1270 1340 1760 1891 Size: 569 116 370 59 156 70 420 131 Cuts at: 1891 1904 2139 3030 Size: 13 235 891 Fragments arranged by size: 891 569 420 370 235 156 131 116 70 59 13 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1064 1219 3030 Size: 1064 155 1811 Fragments arranged by size: 1811 1064 155 BsaAI yAC'GTr Cuts at: 0 2323 3030 Size: 2323 707 BsaWI w'CCGG_w Cuts at: 0 2800 3030 Size: 2800 230 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 575 605 2795 2825 3030 Size: 575 30 2190 30 205 Fragments arranged by size: 2190 575 205 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1905 3030 Size: 1905 1125 BseYI C'CCAG_C Cuts at: 0 238 639 1508 1756 2542 3030 Size: 238 401 869 248 786 488 Fragments arranged by size: 869 786 488 401 248 238 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 437 948 2486 3030 Size: 437 511 1538 544 Fragments arranged by size: 1538 544 511 437 BsiHKAI G_wGCw'C Cuts at: 0 1695 2824 3030 Size: 1695 1129 206 Fragments arranged by size: 1695 1129 206 Bsp1286I G_dGCh'C Cuts at: 0 1592 1695 2824 3030 Size: 1592 103 1129 206 Fragments arranged by size: 1592 1129 206 103 BspHI T'CATG_A Cuts at: 0 2684 3030 Size: 2684 346 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2468 3030 Size: 2468 562 BsrDI GCAATG_nn' Cuts at: 0 280 1559 1869 3030 Size: 280 1279 310 1161 Fragments arranged by size: 1279 1161 310 280 BsrGI T'GTAC_A Cuts at: 0 510 2826 2985 3030 Size: 510 2316 159 45 Fragments arranged by size: 2316 510 159 45 BssSI C'ACGA_G Cuts at: 0 2303 3030 Size: 2303 727 BstAPI GCAn_nnn'nTGC Cuts at: 0 115 119 1114 3030 Size: 115 4 995 1916 Fragments arranged by size: 1916 995 115 4 BstEII G'GTnAC_C Cuts at: 0 1939 3030 Size: 1939 1091 BstXI CCAn_nnnn'nTGG Cuts at: 0 2044 3030 Size: 2044 986 BstYI r'GATC_y Cuts at: 0 1455 2123 3008 3030 Size: 1455 668 885 22 Fragments arranged by size: 1455 885 668 22 Bsu36I CC'TnA_GG Cuts at: 0 256 719 1073 3030 Size: 256 463 354 1957 Fragments arranged by size: 1957 463 354 256 BtgI C'CryG_G Cuts at: 0 1775 3030 Size: 1775 1255 BtsI GCAGTG_nn' Cuts at: 0 33 113 164 1112 2298 3030 Size: 33 80 51 948 1186 732 Fragments arranged by size: 1186 948 732 80 51 33 DraI TTT'AAA Cuts at: 0 1436 3030 Size: 1436 1594 EaeI y'GGCC_r Cuts at: 0 2180 2972 3030 Size: 2180 792 58 Fragments arranged by size: 2180 792 58 EarI CTCTTCn'nnn_ Cuts at: 0 488 581 1600 2168 2642 2884 3030 Size: 488 93 1019 568 474 242 146 Fragments arranged by size: 1019 568 488 474 242 146 93 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 410 569 685 748 1055 1114 1270 1340 Size: 410 159 116 63 307 59 156 70 Cuts at: 1340 1664 1760 1891 1904 2139 2330 2861 2947 Size: 324 96 131 13 235 191 531 86 Cuts at: 2947 3030 Size: 83 Fragments arranged by size: 531 410 324 307 235 191 159 156 131 116 96 86 83 70 63 59 13 EcoNI CCTnn'n_nnAGG Cuts at: 0 686 2167 3030 Size: 686 1481 863 Fragments arranged by size: 1481 863 686 EcoRI G'AATT_C Cuts at: 0 2690 3030 Size: 2690 340 EcoRV GAT'ATC Cuts at: 0 1329 2256 3030 Size: 1329 927 774 Fragments arranged by size: 1329 927 774 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 592 1423 1477 3030 Size: 592 831 54 1553 Fragments arranged by size: 1553 831 592 54 HaeII r_GCGC'y Cuts at: 0 2210 3030 Size: 2210 820 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 338 370 1313 1345 1679 1711 2417 2449 Size: 338 32 943 32 334 32 706 32 Cuts at: 2449 3030 Size: 581 Fragments arranged by size: 943 706 581 338 334 32 32 32 32 HindIII A'AGCT_T Cuts at: 0 1670 3030 Size: 1670 1360 MfeI C'AATT_G Cuts at: 0 557 2630 3030 Size: 557 2073 400 Fragments arranged by size: 2073 557 400 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 195 1467 1815 2549 3030 Size: 195 1272 348 734 481 Fragments arranged by size: 1272 734 481 348 195 MscI TGG'CCA Cuts at: 0 2182 2974 3030 Size: 2182 792 56 Fragments arranged by size: 2182 792 56 MslI CAynn'nnrTG Cuts at: 0 1862 3030 Size: 1862 1168 MspA1I CmG'CkG Cuts at: 0 460 547 1336 1444 2967 3030 Size: 460 87 789 108 1523 63 Fragments arranged by size: 1523 789 460 108 87 63 NcoI C'CATG_G Cuts at: 0 1775 3030 Size: 1775 1255 NspI r_CATG'y Cuts at: 0 786 1731 2023 2355 3030 Size: 786 945 292 332 675 Fragments arranged by size: 945 786 675 332 292 PasI CC'CwG_GG Cuts at: 0 2129 3030 Size: 2129 901 PciI A'CATG_T Cuts at: 0 1727 2019 3030 Size: 1727 292 1011 Fragments arranged by size: 1727 1011 292 PfoI T'CCnGG_A Cuts at: 0 1032 1175 3030 Size: 1032 143 1855 Fragments arranged by size: 1855 1032 143 PmlI CAC'GTG Cuts at: 0 2323 3030 Size: 2323 707 PsiI TTA'TAA Cuts at: 0 19 3030 Size: 19 3011 PstI C_TGCA'G Cuts at: 0 267 403 2958 3030 Size: 267 136 2555 72 Fragments arranged by size: 2555 267 136 72 PvuII CAG'CTG Cuts at: 0 460 547 1336 1444 2967 3030 Size: 460 87 789 108 1523 63 Fragments arranged by size: 1523 789 460 108 87 63 ScaI AGT'ACT Cuts at: 0 14 1912 2481 3030 Size: 14 1898 569 549 Fragments arranged by size: 1898 569 549 14 SfcI C'TryA_G Cuts at: 0 263 399 1983 2954 3030 Size: 263 136 1584 971 76 Fragments arranged by size: 1584 971 263 136 76 SmlI C'TyrA_G Cuts at: 0 1079 1198 3030 Size: 1079 119 1832 Fragments arranged by size: 1832 1079 119 SphI G_CATG'C Cuts at: 0 786 2355 3030 Size: 786 1569 675 Fragments arranged by size: 1569 786 675 SspI AAT'ATT Cuts at: 0 2948 3030 Size: 2948 82 StuI AGG'CCT Cuts at: 0 499 3030 Size: 499 2531 StyI C'CwwG_G Cuts at: 0 1046 1698 1775 3030 Size: 1046 652 77 1255 Fragments arranged by size: 1255 1046 652 77 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2271 2759 2807 3030 Size: 2271 488 48 223 Fragments arranged by size: 2271 488 223 48 TatI w'GTAC_w Cuts at: 0 12 510 1910 2479 2826 2985 3030 Size: 12 498 1400 569 347 159 45 Fragments arranged by size: 1400 569 498 347 159 45 12 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 455 487 575 607 1082 1114 3030 Size: 455 32 88 32 475 32 1916 Fragments arranged by size: 1916 475 455 88 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 2638 2665 3030 Size: 2638 27 365 Fragments arranged by size: 2638 365 27 XbaI T'CTAG_A Cuts at: 0 858 3030 Size: 858 2172 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 336 365 2044 2719 3030 Size: 336 29 1679 675 311 Fragments arranged by size: 1679 675 336 311 29 XmnI GAAnn'nnTTC Cuts at: 0 376 572 935 1842 2694 2879 3030 Size: 376 196 363 907 852 185 151 Fragments arranged by size: 907 852 376 363 196 185 151 Enzymes that do cut: AcuI AflIII AlfI AlwNI ApaLI ApoI AseI AvaI BamHI BanI BanII BbsI BciVI BglI BglII Bme1580I BmrI BpmI BpuEI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI DraI EaeI EarI Eco57MI EcoNI EcoRI EcoRV FalI HaeII Hin4I HindIII MfeI MmeI MscI MslI MspA1I NcoI NspI PasI PciI PfoI PmlI PsiI PstI PvuII ScaI SfcI SmlI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AloI ApaI AscI AsiSI AvrII BaeI BbeI BbvCI BcgI BclI BfrBI BlpI BmgBI BmtI BplI Bpu10I BsaI BsaBI BsaHI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrFI BssHII BstBI BstZ17I BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoO109I FseI FspI FspAI HincII HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PflMI PmeI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI XhoI XmaI ZraI