(Circular) (Six-base) MAPSORT of: pFN21AE0335.seq Check: 5345 from: 1 to: 7447 pFN21AE0335 7447 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5257 278 Size: 53 83 186 936 3721 2468 Fragments arranged by size: 3721 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2427 4790 1937 Size: 490 2363 4594 Fragments arranged by size: 4594 2363 490 Acc65I G'GTAC_C Cuts at: 3900 4768 3900 Size: 868 6579 AclI AA'CG_TT Cuts at: 1563 5574 5947 1563 Size: 4011 373 3063 Fragments arranged by size: 4011 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2414 2460 4413 4585 5510 6894 7162 1632 Size: 782 46 1953 172 925 1384 268 1917 Fragments arranged by size: 1953 1917 1384 925 782 268 172 46 AfeI AGC'GCT Cuts at: 4022 4022 Size: 7447 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7250 AflIII A'CryG_T Cuts at: 1203 3713 4066 6346 7323 1203 Size: 2510 353 2280 977 1327 Fragments arranged by size: 2510 2280 1327 977 353 AgeI A'CCGG_T Cuts at: 6278 6278 Size: 7447 AhdI GACnn_n'nnGTC Cuts at: 2434 6176 2434 Size: 3742 3705 AleI CACnn'nnGTG Cuts at: 1166 2966 1166 Size: 1800 5647 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2984 3603 1184 Size: 1800 619 5028 Fragments arranged by size: 5028 1800 619 AlwNI CAG_nnn'CTG Cuts at: 2073 2897 2936 5190 6762 2073 Size: 824 39 2254 1572 2758 Fragments arranged by size: 2758 2254 1572 824 39 ApaI G_GGCC'C Cuts at: 2930 2930 Size: 7447 ApaLI G'TGCA_C Cuts at: 2969 5504 6660 2969 Size: 2535 1156 3756 Fragments arranged by size: 3756 2535 1156 ApoI r'AATT_y Cuts at: 1493 2223 2464 2597 2606 3792 4756 4982 5134 Size: 730 241 133 9 1186 964 226 152 Cuts at: 5134 7243 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1186 964 730 241 226 152 133 9 AseI AT'TA_AT Cuts at: 160 1032 6001 160 Size: 872 4969 1606 Fragments arranged by size: 4969 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7447 AvaI C'yCGr_G Cuts at: 1870 1943 4772 1870 Size: 73 2829 4545 Fragments arranged by size: 4545 2829 73 BamHI G'GATC_C Cuts at: 2685 4777 5144 2685 Size: 2092 367 4988 Fragments arranged by size: 4988 2092 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3746 3900 4768 6223 618 Size: 325 217 627 1959 154 868 1455 1842 Fragments arranged by size: 1959 1842 1455 868 627 325 217 154 BanII G_rGCy'C Cuts at: 1391 2930 3624 3969 4766 1391 Size: 1539 694 345 797 4072 Fragments arranged by size: 4072 1539 797 694 345 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2172 3028 3405 928 Size: 971 273 856 377 4970 Fragments arranged by size: 4970 971 856 377 273 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5638 5672 1903 Size: 34 3701 34 3678 Fragments arranged by size: 3701 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2762 3089 5339 6549 2762 Size: 327 2250 1210 3660 Fragments arranged by size: 3660 2250 1210 327 BclI T'GATC_A Cuts at: 1548 4527 1548 Size: 2979 4468 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6058 136 Size: 107 122 71 631 605 4386 1525 Fragments arranged by size: 4386 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2575 2954 4584 7442 2575 Size: 379 1630 2858 2580 Fragments arranged by size: 2858 2580 1630 379 BlpI GC'TnA_GC Cuts at: 4864 4864 Size: 7447 Bme1580I G_kGCm'C Cuts at: 1790 2930 2973 5508 6664 1790 Size: 1140 43 2535 1156 2573 Fragments arranged by size: 2573 2535 1156 1140 43 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7447 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2840 2924 3837 Size: 416 53 83 399 24 1423 84 913 Cuts at: 3837 4286 4738 5178 6136 442 Size: 449 452 440 958 1753 Fragments arranged by size: 1753 1423 958 913 452 449 440 416 399 84 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7447 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4723 4723 Size: 7447 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3930 4718 6107 1239 Size: 2691 788 1389 2579 Fragments arranged by size: 2691 2579 1389 788 Bpu10I CC'TnA_GC Cuts at: 3278 4691 3278 Size: 1413 6034 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5572 6437 6735 5572 Size: 865 298 6284 Fragments arranged by size: 6284 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2382 3431 3691 6110 882 Size: 617 883 1049 260 2419 2219 Fragments arranged by size: 2419 2219 1049 883 617 260 BsaAI yAC'GTr Cuts at: 493 1204 4069 4668 5238 493 Size: 711 2865 599 570 2702 Fragments arranged by size: 2865 2702 711 599 570 BsaBI GATnn'nnATC Cuts at: 7441 7441 Size: 7447 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2098 5254 5636 6271 Size: 53 83 186 936 565 3156 382 635 Cuts at: 6271 275 Size: 1451 Fragments arranged by size: 3156 1451 936 635 565 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2299 5880 6278 6552 6699 7110 2299 Size: 3581 398 274 147 411 2636 Fragments arranged by size: 3581 2636 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2799 2829 1178 Size: 30 165 30 1396 30 5796 Fragments arranged by size: 5796 1396 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2265 2549 2552 3421 1186 Size: 1079 284 3 869 5212 Fragments arranged by size: 5212 1079 869 284 3 BseYI C'CCAG_C Cuts at: 4153 4562 6650 7146 4153 Size: 409 2088 496 4454 Fragments arranged by size: 4454 2088 496 409 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6874 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4897 5658 6686 1540 Size: 275 186 2896 761 1028 2301 Fragments arranged by size: 2896 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2973 3207 3615 3624 5508 5593 6664 2973 Size: 234 408 9 1884 85 1071 3756 Fragments arranged by size: 3756 1884 1071 408 234 85 9 BsmI GAATG_Cn' Cuts at: 2866 3592 4958 5051 2866 Size: 726 1366 93 5262 Fragments arranged by size: 5262 1366 726 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2930 2973 3207 3615 3624 3969 4766 Size: 399 1140 43 234 408 9 345 797 Cuts at: 4766 5508 5593 6664 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1140 1071 797 742 408 399 345 234 85 43 9 BspEI T'CCGG_A Cuts at: 2299 7110 2299 Size: 4811 2636 BspHI T'CATG_A Cuts at: 4086 5336 7066 4086 Size: 1250 1730 4467 Fragments arranged by size: 4467 1730 1250 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4802 844 Size: 3958 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5334 1394 Size: 429 3511 3507 Fragments arranged by size: 3511 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4716 5942 6116 65 Size: 1183 3468 1226 174 1396 Fragments arranged by size: 3468 1396 1226 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6091 6278 1700 Size: 110 4281 187 2869 Fragments arranged by size: 4281 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7447 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7447 BssSI C'ACGA_G Cuts at: 5507 6519 5507 Size: 1012 6435 BstAPI GCAn_nnn'nTGC Cuts at: 794 3149 3274 3577 4492 794 Size: 2355 125 303 915 3749 Fragments arranged by size: 3749 2355 915 303 125 BstBI TT'CG_AA Cuts at: 6251 6251 Size: 7447 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7447 BstXI CCAn_nnnn'nTGG Cuts at: 3193 3193 Size: 7447 BstYI r'GATC_y Cuts at: 1972 2575 2685 2954 3506 3831 4287 4584 4777 Size: 603 110 269 552 325 456 297 193 Cuts at: 4777 5144 5529 5546 6998 7084 7096 7442 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 603 552 456 385 367 346 325 297 269 193 110 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3931 513 Size: 552 939 1927 4029 Fragments arranged by size: 4029 1927 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6708 Fragments arranged by size: 6708 683 56 BtsI GCAGTG_nn' Cuts at: 2022 3150 4963 5757 5777 2022 Size: 1128 1813 794 20 3692 Fragments arranged by size: 3692 1813 1128 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7447 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4186 4221 529 Size: 35 3622 35 3755 Fragments arranged by size: 3755 3622 35 35 DraI TTT'AAA Cuts at: 4751 4751 Size: 7447 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4733 6454 809 Size: 507 3417 1721 1802 Fragments arranged by size: 3417 1802 1721 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3419 3934 4456 Size: 54 1425 50 275 18 1589 515 522 Cuts at: 4456 4894 5783 7412 8 Size: 438 889 1629 43 Fragments arranged by size: 1629 1589 1425 889 522 515 438 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4894 1537 Size: 275 3082 4090 Fragments arranged by size: 4090 3082 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2262 2439 2586 3527 5377 1353 Size: 909 177 147 941 1850 3423 Fragments arranged by size: 3423 1850 941 909 177 147 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6034 6408 6554 387 Size: 1064 138 4445 374 146 1280 Fragments arranged by size: 4445 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3622 3622 Size: 7447 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2414 2460 3930 4413 4585 4718 5510 Size: 393 782 46 1470 483 172 133 792 Cuts at: 5510 6107 6894 7162 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1470 792 787 782 597 483 393 268 172 133 46 EcoO109I rG'GnC_Cy Cuts at: 3017 3017 Size: 7447 EcoRI G'AATT_C Cuts at: 4756 5134 4756 Size: 378 7069 EcoRV GAT'ATC Cuts at: 2617 2617 Size: 7447 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2446 3241 2446 Size: 795 6652 FspI TGC'GCA Cuts at: 5953 5953 Size: 7447 HaeII r_GCGC'y Cuts at: 1678 4024 6594 1678 Size: 2346 2570 2531 Fragments arranged by size: 2570 2531 2346 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2420 2452 2528 2560 3996 Size: 32 135 32 848 32 76 32 1436 Cuts at: 3996 4028 4243 4275 6088 6120 6162 6194 1373 Size: 32 215 32 1813 32 42 32 2626 Fragments arranged by size: 2626 1813 1436 848 215 135 76 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4791 5037 669 Size: 988 281 2853 246 3079 Fragments arranged by size: 3079 2853 988 281 246 HindIII A'AGCT_T Cuts at: 748 3296 748 Size: 2548 4899 HpaI GTT'AAC Cuts at: 5037 5037 Size: 7447 KpnI G_GTAC'C Cuts at: 3904 4772 3904 Size: 868 6579 MfeI C'AATT_G Cuts at: 3604 5046 3604 Size: 1442 6005 MluI A'CGCG_T Cuts at: 7323 7323 Size: 7447 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6561 6745 1305 Size: 5256 184 2007 Fragments arranged by size: 5256 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3421 3936 4458 10 Size: 54 1425 343 1589 515 522 2999 Fragments arranged by size: 2999 1589 1425 522 515 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2679 2966 5405 5764 5923 518 Size: 648 1513 287 2439 359 159 2042 Fragments arranged by size: 2439 2042 1513 648 359 287 159 MspA1I CmG'CkG Cuts at: 1606 3111 4863 5540 6688 6933 1606 Size: 1505 1752 677 1148 245 2120 Fragments arranged by size: 2120 1752 1505 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5956 Fragments arranged by size: 5956 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7447 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7447 NotI GC'GGCC_GC Cuts at: 4894 4894 Size: 7447 NspI r_CATG'y Cuts at: 1132 1229 2699 4153 4805 6350 1132 Size: 97 1470 1454 652 1545 2229 Fragments arranged by size: 2229 1545 1470 1454 652 97 PasI CC'CwG_GG Cuts at: 1348 2470 1348 Size: 1122 6325 PciI A'CATG_T Cuts at: 6346 6346 Size: 7447 PflMI CCAn_nnn'nTGG Cuts at: 1108 2830 4374 4728 1108 Size: 1722 1544 354 3827 Fragments arranged by size: 3827 1722 1544 354 PfoI T'CCnGG_A Cuts at: 4219 4219 Size: 7447 PmeI GTTT'AAAC Cuts at: 4751 4751 Size: 7447 PmlI CAC'GTG Cuts at: 4069 4069 Size: 7447 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5516 5548 7029 7061 5516 Size: 32 1481 32 5902 Fragments arranged by size: 5902 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3017 3017 Size: 7447 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7447 PsiI TTA'TAA Cuts at: 2751 5017 2751 Size: 2266 5181 PspOMI G'GGCC_C Cuts at: 2926 2926 Size: 7447 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4037 4069 4037 Size: 32 7415 PstI C_TGCA'G Cuts at: 830 2902 3664 4799 830 Size: 2072 762 1135 3478 Fragments arranged by size: 3478 2072 1135 762 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7447 SacI G_AGCT'C Cuts at: 3624 3624 Size: 7447 SalI G'TCGA_C Cuts at: 1936 4789 1936 Size: 2853 4594 SbfI CC_TGCA'GG Cuts at: 4799 4799 Size: 7447 ScaI AGT'ACT Cuts at: 3047 5695 7407 3047 Size: 2648 1712 3087 Fragments arranged by size: 3087 2648 1712 SexAI A'CCwGG_T Cuts at: 2174 2174 Size: 7447 SfcI C'TryA_G Cuts at: 826 1045 2049 2495 2841 2898 3660 4356 4795 Size: 219 1004 446 346 57 762 696 439 Cuts at: 4795 5930 6611 6802 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1004 762 696 681 446 439 346 219 191 57 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7447 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7447 SmaI CCC'GGG Cuts at: 1872 4774 1872 Size: 2902 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5551 6452 6714 820 Size: 197 926 3608 901 262 1553 Fragments arranged by size: 3608 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4668 5238 493 Size: 4175 570 2702 Fragments arranged by size: 4175 2702 570 SpeI A'CTAG_T Cuts at: 152 152 Size: 7447 SphI G_CATG'C Cuts at: 1132 4805 1132 Size: 3673 3774 SspI AAT'ATT Cuts at: 5 52 5371 5 Size: 47 5319 2081 Fragments arranged by size: 5319 2081 47 StuI AGG'CCT Cuts at: 1517 4093 1517 Size: 2576 4871 StyI C'CwwG_G Cuts at: 513 1065 2004 3456 4299 4886 513 Size: 552 939 1452 843 587 3074 Fragments arranged by size: 3074 1452 939 843 587 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4424 5474 5491 5644 5829 6274 325 Size: 1255 2844 1050 17 153 185 445 1498 Fragments arranged by size: 2844 1498 1255 1050 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2907 3045 4277 Size: 274 80 33 51 1443 930 138 1232 Cuts at: 4277 5693 7405 96 Size: 1416 1712 138 Fragments arranged by size: 1712 1443 1416 1232 930 274 138 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2595 2627 4058 4090 4259 4291 7407 7439 2595 Size: 32 1431 32 169 32 3116 32 2603 Fragments arranged by size: 3116 2603 1431 169 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7447 XbaI T'CTAG_A Cuts at: 4783 4783 Size: 7447 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4570 4637 4693 4570 Size: 67 56 7324 Fragments arranged by size: 7324 67 56 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7447 XmaI C'CCGG_G Cuts at: 1870 4772 1870 Size: 2902 4545 XmnI GAAnn'nnTTC Cuts at: 5576 5576 Size: 7447 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5255 276 Size: 53 83 186 936 3721 2468 Fragments arranged by size: 3721 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI