Restriction map for
pFN21AE0609
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# NO CUT ENZYMES ( 174 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmAI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeIII MamI MfeI MflI
MluI MroI MslI MspA1I MunI
MvnI MwoI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI TaqI Tsp45I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AciI 356 GCGG CCGC(-3/-1)
AcsI 403 GAATTT [GA]^AATT[CT]
AfaI 223 GTAC GT^AC
AlwNI 67 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 403 GAATTT [GA]^AATT[CT]
AspHI 396 GAGCAC G[AT]GC[AT]^C
BalI 2 TGGCCA TGG^CCA
BcnI 219 CCCGG CC^[GC]GG
BfaI 148 CTAG C^TAG
BmyI 396 GAGCAC G[AGT]GC[ACT]^C
BpmI 55 CTGGAG CTGGAG(16/14)
BseRI 58 GAGGAG GAGGAG(10/8)
BsgI 233 GTGCAG GTGCAG(16/14)
BsiHKAI 396 GAGCAC G[AT]GC[AT]^C
Bsp1286I 396 GAGCAC G[AGT]GC[ACT]^C
BspLU11I 373 ACATGT A^CATGT
BspMI 235 GCAGGT ACCTGC(4/8)
BsrI 72 ACTGG ACTGG(1/-1)
BstXI 250 CCAGAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 315 GCAAGC GC[ACGT]^[ACGT]GC
DdeI 394 CTGAG C^T[ACGT]AG
DraI 469 TTTAAA TTT^AAA
DraII 214 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 127 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 137 CCGTGG C^C[GA][CT]GG
EaeI 2 TGGCCA [CT]^GGCC[GA]
EarI 190 GAAGAG CTCTTC(1/4)
EcoO109I 214 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 310 CCTTGG C^C[AT][AT]GG
HapII 220 CCGG C^CGG
HpaII 220 CCGG C^CGG
Ksp632I 190 GAAGAG CTCTTC(1/4)
MaeI 148 CTAG C^TAG
MaeII 35 ACGT A^CGT
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MseI 470 TTAA T^TAA
MspI 220 CCGG C^CGG
NciI 219 CCCGG CC^[GC]GG
NspI 373 ACATGT [GA]CATG^[CT]
PpuMI 214 GGGACCC [GA]G^G[AT]CC[CT]
RsaI 223 GTAC GT^AC
SmlI 112 CTTGAG C^T[CT][GA]AG
SspI 434 AATATT AAT^ATT
StyI 310 CCTTGG C^C[AT][AT]GG
Tru9I 470 TTAA T^TAA
Tsp509I 404 AATT ^AATT
TspRI 341 CACTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AflIII 35 ACGTGT A^C[GA][CT]GT
373 ACATGT
AluI 318 AGCT AG^CT
494 AGCT
BbvI 66 GCAGC GCAGC(8/12)
319 GCTGC
BsiYI 93 CCTCTACAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
218 CCCCGGTACGG
BslI 93 CCTCTACAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
218 CCCCGGTACGG
BsmI 322 GCATTC GAATGC(1/-1)
454 GCATTC
DpnII 168 GATC ^GATC
280 GATC
Fnu4HI 66 GCAGC GC^[ACGT]GC
319 GCTGC
HaeIII 3 GGCC GG^CC
248 GGCC
ItaI 66 GCAGC GC^[ACGT]GC
319 GCTGC
MboI 168 GATC ^GATC
280 GATC
NdeII 168 GATC ^GATC
280 GATC
Sau3AI 168 GATC ^GATC
280 GATC
SexAI 128 ACCTGGT A^CC[AT]GGT
183 ACCAGGT
SfcI 96 CTACAG C^T[GA][CT]AG
120 CTACAG
TseI 66 GCAGC G^C[AT]GC
319 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AvaII BsaJI BsmFI BssKI BstF5I
BstNI Cfr13I EcoRII FokI HinfI
HphI MboII MnlI MvaI NlaIII
NlaIV PleI Sau96I ScrFI TfiI