################################## # NO CUT ENZYMES ( 182 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI AluI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BfrI BglI BlnI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI ItaI KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MseI MslI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I TseI TspRI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AciI 71 CCGC CCGC(-3/-1) AcsI 317 AAATTC [GA]^AATT[CT] AlwI 192 GATCC GGATC(4/5) ApoI 317 AAATTC [GA]^AATT[CT] BanII 220 GGGCTC G[GA]GC[CT]^C BcnI 84 CCGGG CC^[GC]GG BfaI 196 CTAG C^TAG BglII 294 AGATCT A^GATCT BmyI 220 GGGCTC G[AGT]GC[ACT]^C BpmI 62 CTCCAG CTGGAG(16/14) BsmBI 268 GAGACG CGTCTC(1/5) Bsp1286I 220 GGGCTC G[AGT]GC[ACT]^C BspMI 75 ACCTGC ACCTGC(4/8) BstEII 157 GGTGACC G^GT[ACGT]ACC BstF5I 68 CATCC GGATG(2/0) BstPI 157 GGTGACC G^GT[ACGT]ACC DraII 129 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoO109I 129 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoO65I 157 GGTGACC G^GT[ACGT]ACC EcoT14I 323 CCAAGG C^C[AT][AT]GG FokI 68 CATCC GGATG(9/13) HaeIII 210 GGCC GG^CC HapII 84 CCGG C^CGG HinfI 60 GACTC G^A[ACGT]TC HpaII 84 CCGG C^CGG HphI 157 GGTGA GGTGA(8/7) MaeI 196 CTAG C^TAG MaeII 271 ACGT A^CGT MaeIII 158 GTGAC ^GT[ACGT]AC MboII 331 GAAGA GAAGA(8/7) MspI 84 CCGG C^CGG MwoI 302 GCATGCTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 84 CCGGG CC^[GC]GG NspI 302 GCATGC [GA]CATG^[CT] PflMI 283 CCAGAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 60 GACTC GAGTC(4/5) PpuMI 129 GGGTCCC [GA]G^G[AT]CC[CT] SfaNI 67 GCATC GCATC(5/9) SmlI 187 CTTGAG C^T[CT][GA]AG SphI 302 GCATGC GCATG^C StyI 323 CCAAGG C^C[AT][AT]GG TaqI 215 TCGA T^CGA Tsp45I 158 GTGAC ^GT[GC]AC Tsp509I 318 AATT ^AATT TthHB8I 215 TCGA T^CGA Van91I 283 CCAGAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### BlpI 45 GCTGAGC GC^T[ACGT]AGC 306 GCTCAGC Bpu1102I 45 GCTGAGC GC^T[ACGT]AGC 306 GCTCAGC BstNI 21 CCTGG CC^[AT]GG 51 CCTGG BstYI 191 AGATCC [GA]^GATC[CT] 294 AGATCT Cac8I 115 GCTGGC GC[ACGT]^[ACGT]GC 302 GCATGC CelII 45 GCTGAGC GC^T[ACGT]AGC 306 GCTCAGC DdeI 46 CTGAG C^T[ACGT]AG 307 CTCAG DpnII 192 GATC ^GATC 295 GATC EcoNI 201 CCTGTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 248 CCTCCAAGAGG EcoRII 21 CCTGG ^CC[AT]GG 51 CCTGG MboI 192 GATC ^GATC 295 GATC MflI 191 AGATCC [GA]^GATC[CT] 294 AGATCT MvaI 21 CCTGG CC^[AT]GG 51 CCTGG NdeII 192 GATC ^GATC 295 GATC NlaIII 303 CATG CATG^ 312 CATG Sau3AI 192 GATC ^GATC 295 GATC XhoII 191 AGATCC [GA]^GATC[CT] 294 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 13 enzymes) # ######################################### AvaII BsaJI BseRI BsiYI BslI BsmAI BsmFI BssKI Cfr13I MnlI NlaIV Sau96I ScrFI