(Circular) (Six-base) MAPSORT of: pFN21AE0108.seq Check: 1696 from: 1 to: 5659 pFN21AE0108 5659 bp With 182 enzymes: SgfI * October 20, 2009 10:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2296 2805 3469 278 Size: 53 83 186 936 760 509 664 2468 Fragments arranged by size: 2468 936 760 664 509 186 83 53 AccI GT'mk_AC Cuts at: 1937 3002 1937 Size: 1065 4594 Acc65I G'GTAC_C Cuts at: 2018 2980 2018 Size: 962 4697 AclI AA'CG_TT Cuts at: 1563 3786 4159 1563 Size: 2223 373 3063 Fragments arranged by size: 3063 2223 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2615 2918 3722 5106 5374 1632 Size: 983 303 804 1384 268 1917 Fragments arranged by size: 1917 1384 983 804 303 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5462 AflIII A'CryG_T Cuts at: 1203 4558 5535 1203 Size: 3355 977 1327 Fragments arranged by size: 3355 1327 977 AgeI A'CCGG_T Cuts at: 2010 4490 2010 Size: 2480 3179 AhdI GACnn_n'nnGTC Cuts at: 4388 4388 Size: 5659 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5659 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2205 2743 1184 Size: 1021 538 4100 Fragments arranged by size: 4100 1021 538 AlwNI CAG_nnn'CTG Cuts at: 3402 4974 3402 Size: 1572 4087 ApaLI G'TGCA_C Cuts at: 3716 4872 3716 Size: 1156 4503 ApoI r'AATT_y Cuts at: 1493 2149 2233 2340 2968 3194 3346 5455 1493 Size: 656 84 107 628 226 152 2109 1697 Fragments arranged by size: 2109 1697 656 628 226 152 107 84 AseI AT'TA_AT Cuts at: 160 1032 4213 160 Size: 872 3181 1606 Fragments arranged by size: 3181 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5659 AvaI C'yCGr_G Cuts at: 1870 1943 2984 1870 Size: 73 1041 4545 Fragments arranged by size: 4545 1041 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2751 2784 2751 Size: 33 5626 BamHI G'GATC_C Cuts at: 2892 2989 3356 2892 Size: 97 367 5195 Fragments arranged by size: 5195 367 97 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2018 2189 2980 4435 618 Size: 325 217 627 231 171 791 1455 1842 Fragments arranged by size: 1842 1455 791 627 325 231 217 171 BanII G_rGCy'C Cuts at: 1391 2978 1391 Size: 1587 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2176 928 Size: 971 277 4411 Fragments arranged by size: 4411 971 277 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3850 3884 1903 Size: 34 1913 34 3678 Fragments arranged by size: 3678 1913 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3551 4761 3551 Size: 1210 4449 BclI T'GATC_A Cuts at: 1548 1548 Size: 5659 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4270 136 Size: 107 122 71 631 605 2598 1525 Fragments arranged by size: 2598 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5654 5654 Size: 5659 BlpI GC'TnA_GC Cuts at: 2492 3076 2492 Size: 584 5075 Bme1580I G_kGCm'C Cuts at: 1790 2194 3720 4876 1790 Size: 404 1526 1156 2573 Fragments arranged by size: 2573 1526 1156 404 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5659 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2602 2726 3390 Size: 416 53 83 399 24 1185 124 664 Cuts at: 3390 4348 442 Size: 958 1753 Fragments arranged by size: 1753 1185 958 664 416 399 124 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5659 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2933 2933 Size: 5659 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2162 2736 4319 1239 Size: 923 574 1583 2579 Fragments arranged by size: 2579 1583 923 574 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2459 3784 4649 4947 2459 Size: 1325 865 298 3171 Fragments arranged by size: 3171 1325 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2473 4322 882 Size: 617 974 1849 2219 Fragments arranged by size: 2219 1849 974 617 BsaAI yAC'GTr Cuts at: 493 1204 3450 493 Size: 711 2246 2702 Fragments arranged by size: 2702 2246 711 BsaBI GATnn'nnATC Cuts at: 5653 5653 Size: 5659 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2293 2802 3466 3848 Size: 53 83 186 936 760 509 664 382 Cuts at: 3848 4483 275 Size: 635 1451 Fragments arranged by size: 1451 936 760 664 635 509 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2010 4092 4490 4764 4911 5322 2010 Size: 2082 398 274 147 411 2347 Fragments arranged by size: 2347 2082 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5434 Fragments arranged by size: 5434 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2165 1186 Size: 979 4680 BseYI C'CCAG_C Cuts at: 2193 4862 5358 2193 Size: 2669 496 2494 Fragments arranged by size: 2669 2494 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2621 1163 Size: 573 885 4201 Fragments arranged by size: 4201 885 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3109 3870 4898 1540 Size: 275 186 1108 761 1028 2301 Fragments arranged by size: 2301 1108 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3720 3805 4876 3720 Size: 85 1071 4503 Fragments arranged by size: 4503 1071 85 BsmI GAATG_Cn' Cuts at: 3170 3263 3170 Size: 93 5566 BsmBI CGTCTCn'nnnn_ Cuts at: 2284 2284 Size: 5659 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2194 2978 3720 3805 4876 1391 Size: 399 404 784 742 85 1071 2174 Fragments arranged by size: 2174 1071 784 742 404 399 85 BspEI T'CCGG_A Cuts at: 5322 5322 Size: 5659 BspHI T'CATG_A Cuts at: 3548 5278 3548 Size: 1730 3929 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3014 844 Size: 2170 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2884 3546 1394 Size: 429 1061 662 3507 Fragments arranged by size: 3507 1061 662 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2080 2732 4154 4328 65 Size: 1183 832 652 1422 174 1396 Fragments arranged by size: 1422 1396 1183 832 652 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2010 4303 4490 1700 Size: 110 200 2293 187 2869 Fragments arranged by size: 2869 2293 200 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5659 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5659 BssSI C'ACGA_G Cuts at: 3719 4731 3719 Size: 1012 4647 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5659 BstBI TT'CG_AA Cuts at: 2344 4463 2344 Size: 2119 3540 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5659 BstXI CCAn_nnnn'nTGG Cuts at: 2186 2186 Size: 5659 BstYI r'GATC_y Cuts at: 1972 2892 2989 3356 3741 3758 5210 5296 5308 Size: 920 97 367 385 17 1452 86 12 Cuts at: 5308 5654 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 920 385 367 346 97 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2037 2952 513 Size: 552 972 915 3220 Fragments arranged by size: 3220 972 915 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2230 487 Size: 683 56 1004 3916 Fragments arranged by size: 3916 1004 683 56 BtsI GCAGTG_nn' Cuts at: 2727 3175 3969 3989 2727 Size: 448 794 20 4397 Fragments arranged by size: 4397 794 448 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5659 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5624 DraI TTT'AAA Cuts at: 2963 2963 Size: 5659 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4666 809 Size: 507 3350 1802 Fragments arranged by size: 3350 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3106 3995 5624 Size: 54 1425 50 275 18 1276 889 1629 Cuts at: 5624 8 Size: 43 Fragments arranged by size: 1629 1425 1276 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3106 1537 Size: 275 1294 4090 Fragments arranged by size: 4090 1294 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2266 2581 3589 1353 Size: 913 315 1008 3423 Fragments arranged by size: 3423 1008 913 315 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2680 4246 4620 4766 387 Size: 1064 138 1091 1566 374 146 1280 Fragments arranged by size: 1566 1280 1091 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2162 2615 2736 2918 3722 4319 5106 Size: 393 530 453 121 182 804 597 787 Cuts at: 5106 5374 1239 Size: 268 1524 Fragments arranged by size: 1524 804 787 597 530 453 393 268 182 121 EcoRI G'AATT_C Cuts at: 2233 2340 2968 3346 2233 Size: 107 628 378 4546 Fragments arranged by size: 4546 628 378 107 EcoRV GAT'ATC Cuts at: 2113 2113 Size: 5659 FspI TGC'GCA Cuts at: 4165 4165 Size: 5659 HaeII r_GCGC'y Cuts at: 1678 4806 1678 Size: 3128 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2208 2240 2641 2673 4300 Size: 32 135 32 636 32 401 32 1627 Cuts at: 4300 4332 4374 4406 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1627 636 401 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3003 3249 669 Size: 988 281 1065 246 3079 Fragments arranged by size: 3079 1065 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5659 HpaI GTT'AAC Cuts at: 3249 3249 Size: 5659 KpnI G_GTAC'C Cuts at: 2022 2984 2022 Size: 962 4697 MfeI C'AATT_G Cuts at: 3258 3258 Size: 5659 MluI A'CGCG_T Cuts at: 5535 5535 Size: 5659 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2251 2833 4773 4957 1305 Size: 946 582 1940 184 2007 Fragments arranged by size: 2007 1940 946 582 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3837 Fragments arranged by size: 3837 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3617 3976 4135 518 Size: 648 2451 359 159 2042 Fragments arranged by size: 2451 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3075 3752 4900 5145 1606 Size: 1469 677 1148 245 2120 Fragments arranged by size: 2120 1469 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5107 NdeI CA'TA_TG Cuts at: 387 387 Size: 5659 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5659 NotI GC'GGCC_GC Cuts at: 3106 3106 Size: 5659 NspI r_CATG'y Cuts at: 1132 1229 3017 4562 1132 Size: 97 1788 1545 2229 Fragments arranged by size: 2229 1788 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5659 PciI A'CATG_T Cuts at: 4558 4558 Size: 5659 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5659 PfoI T'CCnGG_A Cuts at: 2713 2713 Size: 5659 PmeI GTTT'AAAC Cuts at: 2963 2963 Size: 5659 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3728 3760 5241 5273 3728 Size: 32 1481 32 4114 Fragments arranged by size: 4114 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5659 PsiI TTA'TAA Cuts at: 3229 3229 Size: 5659 PstI C_TGCA'G Cuts at: 830 2835 3011 830 Size: 2005 176 3478 Fragments arranged by size: 3478 2005 176 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5659 SalI G'TCGA_C Cuts at: 1936 3001 1936 Size: 1065 4594 SbfI CC_TGCA'GG Cuts at: 3011 3011 Size: 5659 ScaI AGT'ACT Cuts at: 3907 5619 3907 Size: 1712 3947 SfcI C'TryA_G Cuts at: 826 1045 2831 3007 4142 4823 5014 826 Size: 219 1786 176 1135 681 191 1471 Fragments arranged by size: 1786 1471 1135 681 219 191 176 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5659 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5659 SmaI CCC'GGG Cuts at: 1872 2986 1872 Size: 1114 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2474 3763 4664 4926 820 Size: 197 926 531 1289 901 262 1553 Fragments arranged by size: 1553 1289 926 901 531 262 197 SnaBI TAC'GTA Cuts at: 493 3450 493 Size: 2957 2702 SpeI A'CTAG_T Cuts at: 152 2252 152 Size: 2100 3559 SphI G_CATG'C Cuts at: 1132 3017 1132 Size: 1885 3774 SspI AAT'ATT Cuts at: 5 52 2402 3583 5 Size: 47 2350 1181 2081 Fragments arranged by size: 2350 2081 1181 47 StuI AGG'CCT Cuts at: 1517 2592 1517 Size: 1075 4584 StyI C'CwwG_G Cuts at: 513 1065 3098 513 Size: 552 2033 3074 Fragments arranged by size: 3074 2033 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3686 3703 3856 4041 4486 325 Size: 1255 2106 17 153 185 445 1498 Fragments arranged by size: 2106 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3905 5617 96 Size: 274 80 33 51 1443 1928 1712 138 Fragments arranged by size: 1928 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5619 5651 5619 Size: 32 5627 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5659 XbaI T'CTAG_A Cuts at: 2995 2995 Size: 5659 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2234 2234 Size: 5659 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5659 XmaI C'CCGG_G Cuts at: 1870 2984 1870 Size: 1114 4545 XmnI GAAnn'nnTTC Cuts at: 3788 3788 Size: 5659 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2294 2803 3467 276 Size: 53 83 186 936 760 509 664 2468 Fragments arranged by size: 2468 936 760 664 509 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI