Restriction map for
pFN21AB6727
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AfaI AflII AflIII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspHI AspI AvaI
AvaII AviII AvrII BanI BanII
BbeI BbrPI BbsI BbvCI BciVI
BclI BfaI BfrI BglI BglII
BlnI BmyI BpmI BpuAI BsaAI
BsaBI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CfoI
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HhaI HinP1I HincII
HindII HindIII HinfI HpaI KasI
KpnI KspI MaeI MaeII MaeIII
MamI MfeI MluI MroI MseI
MslI MspA1I MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI TfiI Tru9I
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcyI 3 GGCGTC G[GA]^CG[CT]C
AhdI 94 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 244 AGCT AG^CT
AlwNI 100 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 94 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 134 GGATCC G^GATCC
BcgI 152 GCATCCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 131 CCGGG CC^[GC]GG
BlpI 165 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 165 GCTGAGC GC^T[ACGT]AGC
BsaHI 3 GGCGTC G[GA]^CG[CT]C
BsaI 37 GGTCTC GGTCTC(1/5)
BsmBI 5 CGTCTC CGTCTC(1/5)
BsmFI 102 GTCCC GGGAC(10/14)
BsrFI 179 GCCGGT [GA]^CCGG[CT]
BstAPI 170 GCAGAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 147 CCTGG CC^[AT]GG
BstYI 134 GGATCC [GA]^GATC[CT]
Cac8I 269 GCAGGC GC[ACGT]^[ACGT]GC
CelII 165 GCTGAGC GC^T[ACGT]AGC
Cfr10I 179 GCCGGT [GA]^CCGG[CT]
DdeI 166 CTGAG C^T[ACGT]AG
DsaI 47 CCATGG C^C[GA][CT]GG
Eam1105I 94 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRII 147 CCTGG ^CC[AT]GG
FauI 266 CCCGC CCCGC(4/6)
HgaI 4 GCGTC GACGC(5/10)
Hin1I 3 GGCGTC G[GA]^CG[CT]C
HphI 44 TCACC GGTGA(8/7)
MflI 134 GGATCC [GA]^GATC[CT]
MvaI 147 CCTGG CC^[AT]GG
NciI 131 CCGGG CC^[GC]GG
NcoI 47 CCATGG C^CATGG
NlaIII 48 CATG CATG^
PflMI 15 CCACATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 106 CTGCAG CTGCA^G
SfcI 106 CTGCAG C^T[GA][CT]AG
StuI 117 AGGCCT AGG^CCT
Van91I 15 CCACATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 134 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 28 enzymes) #
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AlwI 135 GATCC GGATC(4/5)
134 GGATC
BalI 12 TGGCCA TGG^CCA
50 TGGCCA
BbvI 198 GCAGC GCAGC(8/12)
242 GCAGC
BseRI 40 CTCCTC GAGGAG(10/8)
226 GAGGAG
BsmAI 6 GTCTC GTCTC(1/5)
38 GTCTC
BsrI 208 ACTGG ACTGG(1/-1)
99 CCAGT
BssKI 131 CCGGG ^CC[ACGT]GG
147 CCTGG
DpnII 111 GATC ^GATC
135 GATC
EaeI 12 TGGCCA [CT]^GGCC[GA]
50 TGGCCA
EarI 139 CTCTTC CTCTTC(1/4)
223 GAAGAG
EcoT14I 47 CCATGG C^C[AT][AT]GG
53 CCAAGG
Fnu4HI 198 GCAGC GC^[ACGT]GC
242 GCAGC
HapII 131 CCGG C^CGG
180 CCGG
HpaII 131 CCGG C^CGG
180 CCGG
ItaI 198 GCAGC GC^[ACGT]GC
242 GCAGC
Ksp632I 139 CTCTTC CTCTTC(1/4)
223 GAAGAG
MboI 111 GATC ^GATC
135 GATC
MluNI 12 TGGCCA TGG^CCA
50 TGGCCA
MscI 12 TGGCCA TGG^CCA
50 TGGCCA
MspI 131 CCGG C^CGG
180 CCGG
MwoI 170 GCAGAAGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
245 GCTCCATCCGC
NdeII 111 GATC ^GATC
135 GATC
NlaIV 134 GGATCC GG[ACGT]^[ACGT]CC
213 GGAACC
Sau3AI 111 GATC ^GATC
135 GATC
ScrFI 131 CCGGG CC^[ACGT]GG
147 CCTGG
SfaNI 176 GATGC GCATC(5/9)
152 GCATC
StyI 47 CCATGG C^C[AT][AT]GG
53 CCAAGG
TseI 198 GCAGC G^C[AT]GC
242 GCAGC
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# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
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AciI BsaJI BsiYI BslI BstF5I
FokI HaeIII MboII MnlI