Restriction map for
pFN21AE5101
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# NO CUT ENZYMES ( 183 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiHKAI BsiWI BsiYI BslI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FokI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaI KasI
KpnI KspI MaeI MaeII MaeIII
MamI MfeI MluI MroI MseI
MslI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tru9I Tsp45I
Tsp509I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AciI 251 CCGC CCGC(-3/-1)
AluI 60 AGCT AG^CT
AlwNI 103 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 108 CCTGAGG CC^T[ACGT]AGG
ApaI 209 GGGCCC GGGCC^C
AvaI 206 CCCGGG C^[CT]CG[GA]G
BalI 50 TGGCCA TGG^CCA
BamHI 155 GGATCC G^GATCC
BbsI 16 GTCTTC GAAGAC(2/6)
BpuAI 16 GTCTTC GAAGAC(2/6)
BsmFI 120 GGGAC GGGAC(10/14)
BsoBI 206 CCCGGG C^[CT]CG[GA]G
BspMI 176 GCAGGT ACCTGC(4/8)
BstXI 41 CCATGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 155 GGATCC [GA]^GATC[CT]
Bsu36I 108 CCTGAGG CC^T[ACGT]AGG
DpnII 156 GATC ^GATC
DraII 209 GGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 41 CCATGG C^C[GA][CT]GG
EaeI 50 TGGCCA [CT]^GGCC[GA]
EarI 142 CTCTTC CTCTTC(1/4)
Eco81I 108 CCTGAGG CC^T[ACGT]AGG
EcoO109I 209 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 41 CCATGG C^C[AT][AT]GG
HapII 207 CCGG C^CGG
HpaII 207 CCGG C^CGG
HphI 248 TCACC GGTGA(8/7)
Ksp632I 142 CTCTTC CTCTTC(1/4)
MboI 156 GATC ^GATC
MflI 155 GGATCC [GA]^GATC[CT]
MluNI 50 TGGCCA TGG^CCA
MscI 50 TGGCCA TGG^CCA
MspA1I 59 CAGCTG C[AC]G^C[GT]G
MspI 207 CCGG C^CGG
MwoI 52 GCCAGTGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 41 CCATGG C^CATGG
NdeII 156 GATC ^GATC
NlaIII 42 CATG CATG^
PstI 24 CTGCAG CTGCA^G
PvuII 59 CAGCTG CAG^CTG
Sau3AI 156 GATC ^GATC
SfcI 24 CTGCAG C^T[GA][CT]AG
SmaI 206 CCCGGG CCC^GGG
StyI 41 CCATGG C^C[AT][AT]GG
TspRI 54 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 155 GGATCC [GA]^GATC[CT]
XmaCI 206 CCCGGG C^CCGGG
XmaI 206 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AlwI 156 GATCC GGATC(4/5)
155 GGATC
AvaII 45 GGTCC G^G[AT]CC
81 GGACC
BanII 209 GGGCCC G[GA]GC[CT]^C
265 GAGCCC
BbvI 26 GCAGC GCAGC(8/12)
58 GCAGC
BcnI 206 CCCGG CC^[GC]GG
207 CCGGG
BmyI 209 GGGCCC G[AGT]GC[ACT]^C
265 GAGCCC
BsgI 56 GTGCAG GTGCAG(16/14)
186 GTGCAG
Bsp1286I 209 GGGCCC G[AGT]GC[ACT]^C
265 GAGCCC
BsrI 123 ACTGG ACTGG(1/-1)
53 CCAGT
Cac8I 29 GCCTGC GC[ACGT]^[ACGT]GC
33 GCTGGC
DdeI 109 CTGAG C^T[ACGT]AG
169 CTAAG
Fnu4HI 26 GCAGC GC^[ACGT]GC
58 GCAGC
ItaI 26 GCAGC GC^[ACGT]GC
58 GCAGC
MboII 17 TCTTC GAAGA(8/7)
143 TCTTC
NciI 206 CCCGG CC^[GC]GG
207 CCGGG
TseI 26 GCAGC G^C[AT]GC
58 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
BsaJI BssKI BstNI Cfr13I EcoRII
HaeIII MnlI MvaI NlaIV Sau96I
ScrFI