Restriction map for
pFN21AE5101
(Circular) (Six-base) MAPSORT of: pFN21AE5101.seq Check: 6383 from: 1 to: 4990
pFN21AE5101 4990 bp
With 182 enzymes: SgfI *
January 30, 2014 17:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2172 2172
Size: 4990
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2800 278
Size: 53 83 186 936 1264 2468
Fragments arranged by size:
2468 1264 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2333 1937
Size: 396 4594
Acc65I G'GTAC_C
Cuts at: 2311 2311
Size: 4990
AclI AA'CG_TT
Cuts at: 1563 2289 3117 3490 1563
Size: 726 828 373 3063
Fragments arranged by size:
3063 828 726 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3053 4437 4705 1632
Size: 1421 1384 268 1917
Fragments arranged by size:
1917 1421 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4793
AflIII A'CryG_T
Cuts at: 1203 3889 4866 1203
Size: 2686 977 1327
Fragments arranged by size:
2686 1327 977
AgeI A'CCGG_T
Cuts at: 3821 3821
Size: 4990
AhdI GACnn_n'nnGTC
Cuts at: 3719 3719
Size: 4990
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4990
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4990
AlwNI CAG_nnn'CTG
Cuts at: 2113 2733 4305 2113
Size: 620 1572 2798
Fragments arranged by size:
2798 1572 620
ApaI G_GGCC'C
Cuts at: 2218 2218
Size: 4990
ApaLI G'TGCA_C
Cuts at: 3047 4203 3047
Size: 1156 3834
ApoI r'AATT_y
Cuts at: 1493 2299 2525 2677 4786 1493
Size: 806 226 152 2109 1697
Fragments arranged by size:
2109 1697 806 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3544 160
Size: 872 2512 1606
Fragments arranged by size:
2512 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4990
AvaI C'yCGr_G
Cuts at: 1870 1943 2211 2315 1870
Size: 73 268 104 4545
Fragments arranged by size:
4545 268 104 73
BamHI G'GATC_C
Cuts at: 2160 2320 2687 2160
Size: 160 367 4463
Fragments arranged by size:
4463 367 160
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2311 3766 618
Size: 325 217 627 524 1455 1842
Fragments arranged by size:
1842 1455 627 524 325 217
BanII G_rGCy'C
Cuts at: 1391 2218 2274 2309 1391
Size: 827 56 35 4072
Fragments arranged by size:
4072 827 56 35
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2014 928
Size: 971 115 3904
Fragments arranged by size:
3904 971 115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3181 3215 1903
Size: 34 1244 34 3678
Fragments arranged by size:
3678 1244 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2882 4092 2882
Size: 1210 3780
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4990
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3601 136
Size: 107 122 71 631 605 1929 1525
Fragments arranged by size:
1929 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4985 4985
Size: 4990
BlpI GC'TnA_GC
Cuts at: 2407 2407
Size: 4990
Bme1580I G_kGCm'C
Cuts at: 1790 2218 3051 4207 1790
Size: 428 833 1156 2573
Fragments arranged by size:
2573 1156 833 428
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4990
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2721 3679 442
Size: 416 53 83 399 24 1304 958 1753
Fragments arranged by size:
1753 1304 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4990
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3650 1239
Size: 2411 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3115 3980 4278 3115
Size: 865 298 3827
Fragments arranged by size:
3827 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3653 882
Size: 617 2154 2219
Fragments arranged by size:
2219 2154 617
BsaAI yAC'GTr
Cuts at: 493 1204 2781 493
Size: 711 1577 2702
Fragments arranged by size:
2702 1577 711
BsaBI GATnn'nnATC
Cuts at: 4984 4984
Size: 4990
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2797 3179 3814 275
Size: 53 83 186 936 1264 382 635 1451
Fragments arranged by size:
1451 1264 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3423 3821 4095 4242 4653 3423
Size: 398 274 147 411 3760
Fragments arranged by size:
3760 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4765
Fragments arranged by size:
4765 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4990
BseYI C'CCAG_C
Cuts at: 4193 4689 4193
Size: 496 4494
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2082 2212 1163
Size: 573 346 130 3941
Fragments arranged by size:
3941 573 346 130
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2440 3201 4229 1540
Size: 275 186 439 761 1028 2301
Fragments arranged by size:
2301 1028 761 439 275 186
BsiHKAI G_wGCw'C
Cuts at: 3051 3136 4207 3051
Size: 85 1071 3834
Fragments arranged by size:
3834 1071 85
BsmI GAATG_Cn'
Cuts at: 2501 2594 2501
Size: 93 4897
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2218 2274 2309 3051 3136 4207 1391
Size: 399 428 56 35 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 428 399 85 56 35
BspEI T'CCGG_A
Cuts at: 4653 4653
Size: 4990
BspHI T'CATG_A
Cuts at: 2879 4609 2879
Size: 1730 3260
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2172 2345 844
Size: 1328 173 3489
Fragments arranged by size:
3489 1328 173
BsrBI CCG'CTC
Cuts at: 1394 1823 2877 1394
Size: 429 1054 3507
Fragments arranged by size:
3507 1054 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3485 3659 65
Size: 1183 2237 174 1396
Fragments arranged by size:
2237 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3634 3821 1700
Size: 110 1824 187 2869
Fragments arranged by size:
2869 1824 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4990
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4990
BssSI C'ACGA_G
Cuts at: 3050 4062 3050
Size: 1012 3978
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4990
BstBI TT'CG_AA
Cuts at: 3794 3794
Size: 4990
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4990
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2053 2011
Size: 42 4948
BstYI r'GATC_y
Cuts at: 1972 2160 2320 2687 3072 3089 4541 4627 4639
Size: 188 160 367 385 17 1452 86 12
Cuts at: 4639 4985 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 188 160 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2114 2114
Size: 4990
BtgI C'CryG_G
Cuts at: 513 1065 2004 2046 513
Size: 552 939 42 3457
Fragments arranged by size:
3457 939 552 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4251
Fragments arranged by size:
4251 683 56
BtsI GCAGTG_nn'
Cuts at: 2506 3300 3320 2506
Size: 794 20 4176
Fragments arranged by size:
4176 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4990
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4955
DraI TTT'AAA
Cuts at: 2294 2294
Size: 4990
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3997 809
Size: 507 2681 1802
Fragments arranged by size:
2681 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2437 3326
Size: 54 1425 50 275 18 225 382 889
Cuts at: 3326 4955 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 382 275 225 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2437 1537
Size: 275 625 4090
Fragments arranged by size:
4090 625 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2153 2920 1353
Size: 800 767 3423
Fragments arranged by size:
3423 800 767
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3577 3951 4097 387
Size: 1064 138 1988 374 146 1280
Fragments arranged by size:
1988 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3053 3650 4437 4705 1239
Size: 393 1421 597 787 268 1524
Fragments arranged by size:
1524 1421 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2215 2215
Size: 4990
EcoRI G'AATT_C
Cuts at: 2299 2677 2299
Size: 378 4612
FspI TGC'GCA
Cuts at: 3496 3496
Size: 4990
HaeII r_GCGC'y
Cuts at: 1678 4137 1678
Size: 2459 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3631 3663 3705 3737 1373
Size: 32 135 32 2059 32 42 32 2626
Fragments arranged by size:
2626 2059 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2334 2580 669
Size: 988 281 396 246 3079
Fragments arranged by size:
3079 988 396 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4990
HpaI GTT'AAC
Cuts at: 2580 2580
Size: 4990
KpnI G_GTAC'C
Cuts at: 2315 2315
Size: 4990
MfeI C'AATT_G
Cuts at: 2589 2589
Size: 4990
MluI A'CGCG_T
Cuts at: 4866 4866
Size: 4990
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4104 4288 1305
Size: 2799 184 2007
Fragments arranged by size:
2799 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2057 10
Size: 54 1425 343 225 2943
Fragments arranged by size:
2943 1425 343 225 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2948 3307 3466 518
Size: 648 1782 359 159 2042
Fragments arranged by size:
2042 1782 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2066 2406 3083 4231 4476 1606
Size: 460 340 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 460 340 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2046 513
Size: 552 939 42 3457
Fragments arranged by size:
3457 939 552 42
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4990
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4990
NotI GC'GGCC_GC
Cuts at: 2437 2437
Size: 4990
NspI r_CATG'y
Cuts at: 1132 1229 2348 3893 1132
Size: 97 1119 1545 2229
Fragments arranged by size:
2229 1545 1119 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4990
PciI A'CATG_T
Cuts at: 3889 3889
Size: 4990
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4990
PmeI GTTT'AAAC
Cuts at: 2294 2294
Size: 4990
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3059 3091 4572 4604 3059
Size: 32 1481 32 3445
Fragments arranged by size:
3445 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4990
PsiI TTA'TAA
Cuts at: 2560 2560
Size: 4990
PspOMI G'GGCC_C
Cuts at: 2214 2214
Size: 4990
PstI C_TGCA'G
Cuts at: 830 2033 2342 830
Size: 1203 309 3478
Fragments arranged by size:
3478 1203 309
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4990
PvuII CAG'CTG
Cuts at: 2066 2066
Size: 4990
SalI G'TCGA_C
Cuts at: 1936 2332 1936
Size: 396 4594
SbfI CC_TGCA'GG
Cuts at: 2342 2342
Size: 4990
ScaI AGT'ACT
Cuts at: 3238 4950 3238
Size: 1712 3278
SfcI C'TryA_G
Cuts at: 826 1045 2029 2338 3473 4154 4345 826
Size: 219 984 309 1135 681 191 1471
Fragments arranged by size:
1471 1135 984 681 309 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4990
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4990
SmaI CCC'GGG
Cuts at: 1872 2213 2317 1872
Size: 341 104 4545
Fragments arranged by size:
4545 341 104
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3094 3995 4257 820
Size: 197 926 1151 901 262 1553
Fragments arranged by size:
1553 1151 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2781 493
Size: 2288 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4990
SphI G_CATG'C
Cuts at: 1132 2348 1132
Size: 1216 3774
SspI AAT'ATT
Cuts at: 5 52 2914 5
Size: 47 2862 2081
Fragments arranged by size:
2862 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4990
StyI C'CwwG_G
Cuts at: 513 1065 2004 2046 2429 513
Size: 552 939 42 383 3074
Fragments arranged by size:
3074 939 552 383 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3017 3034 3187 3372 3817 325
Size: 1255 1437 17 153 185 445 1498
Fragments arranged by size:
1498 1437 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3236 4948 96
Size: 274 80 33 51 1443 1259 1712 138
Fragments arranged by size:
1712 1443 1259 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4950 4982 4950
Size: 32 4958
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4990
XbaI T'CTAG_A
Cuts at: 2326 2326
Size: 4990
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4990
XmaI C'CCGG_G
Cuts at: 1870 2211 2315 1870
Size: 341 104 4545
Fragments arranged by size:
4545 341 104
XmnI GAAnn'nnTTC
Cuts at: 3119 3119
Size: 4990
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2798 276
Size: 53 83 186 936 1264 2468
Fragments arranged by size:
2468 1264 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI