################################## # NO CUT ENZYMES ( 151 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AfaI AflIII AgeI AhdI Alw44I AlwI AocI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BbrPI BbsI BcgI BciVI BclI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DpnII DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRV FbaI FseI FspI HgaI HincII HindII HindIII HpaI KpnI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MunI NcoI NdeI NdeII NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SalI SapI Sau3AI SbfI ScaI SexAI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccII 93 CGCG CG^CG AcyI 205 GGCGCC G[GA]^CG[CT]C AflII 736 CTTAAG C^TTAAG AlwNI 19 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 66 AGCGCT AGC^GCT BbeI 205 GGCGCC GGCGC^C BbvCI 472 GCTGAGG CCTCAGC(-5/-2) BfrI 736 CTTAAG C^TTAAG BsaHI 205 GGCGCC G[GA]^CG[CT]C BsmFI 336 GTCCC GGGAC(10/14) BsmI 996 GCATTC GAATGC(1/-1) BsrBI 895 GAGCGG CCGCTC(-3/-3) BsrFI 211 GCCGGC [GA]^CCGG[CT] BstUI 93 CGCG CG^CG Cfr10I 211 GCCGGC [GA]^CCGG[CT] EaeI 285 TGGCCG [CT]^GGCC[GA] EarI 564 GAAGAG CTCTTC(1/4) Eco47III 66 AGCGCT AGC^GCT EcoT14I 830 CCTTGG C^C[AT][AT]GG EcoT22I 994 ATGCAT ATGCA^T Hin1I 205 GGCGCC G[GA]^CG[CT]C KasI 205 GGCGCC G^GCGCC Ksp632I 564 GAAGAG CTCTTC(1/4) KspI 92 CCGCGG CCGC^GG MslI 721 CACTGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 93 CGCG CG^CG NaeI 211 GCCGGC GCC^GGC NarI 205 GGCGCC GG^CGCC NgoMIV 211 GCCGGC G^CCGGC NsiI 994 ATGCAT ATGCA^T PflMI 819 CCACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 25 CTGCAG CTGCA^G SacII 92 CCGCGG CCGC^GG SfaNI 586 GCATC GCATC(5/9) SfcI 25 CTGCAG C^T[GA][CT]AG SfiI 237 GGCCGTGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 205 GGCGCC GGC^GCC SmlI 736 CTTAAG C^T[CT][GA]AG StyI 830 CCTTGG C^C[AT][AT]GG TaqI 560 TCGA T^CGA TthHB8I 560 TCGA T^CGA Van91I 819 CCACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 874 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AcsI 316 GAATTC [GA]^AATT[CT] 510 GAATTC ApoI 316 GAATTC [GA]^AATT[CT] 510 GAATTC BfaI 367 CTAG C^TAG 875 CTAG BglI 238 GCCGTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 913 GCCCGACTGGC BseRI 255 GAGGAG GAGGAG(10/8) 483 GAGGAG BsmAI 4 GAGAC GTCTC(1/5) 571 GTCTC EcoRI 316 GAATTC G^AATTC 510 GAATTC HaeII 66 AGCGCT [GA]GCGC^[CT] 205 GGCGCC MaeI 367 CTAG C^TAG 875 CTAG MaeIII 82 GTGAC ^GT[ACGT]AC 418 GTCAC NlaIII 651 CATG CATG^ 744 CATG Tsp45I 82 GTGAC ^GT[GC]AC 418 GTCAC Tsp509I 317 AATT ^AATT 511 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AciI AluI ApaI BanI BanII BbvI BcnI BmyI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DraII DsaI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MboII MnlI MseI MspA1I MspI MvaI MwoI NciI NlaIV Sau96I ScrFI TfiI Tru9I TseI TspRI