(Circular) (Six-base) MAPSORT of: pFN21AB5667.seq Check: 5338 from: 1 to: 5716 pFN21AB5667 5716 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3526 278 Size: 53 83 186 936 1990 2468 Fragments arranged by size: 2468 1990 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3059 1937 Size: 1122 4594 Acc65I G'GTAC_C Cuts at: 3037 3037 Size: 5716 AclI AA'CG_TT Cuts at: 1563 3843 4216 1563 Size: 2280 373 3063 Fragments arranged by size: 3063 2280 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2300 3779 5163 5431 1632 Size: 668 1479 1384 268 1917 Fragments arranged by size: 1917 1479 1384 668 268 AfeI AGC'GCT Cuts at: 2073 2073 Size: 5716 AflII C'TTAA_G Cuts at: 820 1017 2741 820 Size: 197 1724 3795 Fragments arranged by size: 3795 1724 197 AflIII A'CryG_T Cuts at: 1203 4615 5592 1203 Size: 3412 977 1327 Fragments arranged by size: 3412 1327 977 AgeI A'CCGG_T Cuts at: 4547 4547 Size: 5716 AhdI GACnn_n'nnGTC Cuts at: 4445 4445 Size: 5716 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5716 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2954 1184 Size: 1770 3946 AlwNI CAG_nnn'CTG Cuts at: 2029 3459 5031 2029 Size: 1430 1572 2714 Fragments arranged by size: 2714 1572 1430 ApaI G_GGCC'C Cuts at: 2129 2188 2806 2129 Size: 59 618 5039 Fragments arranged by size: 5039 618 59 ApaLI G'TGCA_C Cuts at: 3773 4929 3773 Size: 1156 4560 ApoI r'AATT_y Cuts at: 1493 2321 2515 3025 3251 3403 5512 1493 Size: 828 194 510 226 152 2109 1697 Fragments arranged by size: 2109 1697 828 510 226 194 152 AseI AT'TA_AT Cuts at: 160 1032 4270 160 Size: 872 3238 1606 Fragments arranged by size: 3238 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5716 AvaI C'yCGr_G Cuts at: 1870 1943 3041 1870 Size: 73 1098 4545 Fragments arranged by size: 4545 1098 73 BamHI G'GATC_C Cuts at: 3046 3413 3046 Size: 367 5349 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2045 2210 2642 3037 4492 Size: 325 217 627 258 165 432 395 1455 Cuts at: 4492 618 Size: 1842 Fragments arranged by size: 1842 1455 627 432 395 325 258 217 165 BanII G_rGCy'C Cuts at: 1391 2129 2188 2371 2806 3035 1391 Size: 738 59 183 435 229 4072 Fragments arranged by size: 4072 738 435 229 183 59 BbeI G_GCGC'C Cuts at: 2214 2214 Size: 5716 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4745 BbvCI CC'TCA_GC Cuts at: 2478 2478 Size: 5716 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3907 3941 1903 Size: 34 1970 34 3678 Fragments arranged by size: 3678 1970 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3608 4818 3608 Size: 1210 4506 BclI T'GATC_A Cuts at: 1548 1548 Size: 5716 BfrBI ATG'CAT Cuts at: 3001 3001 Size: 5716 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2249 2924 4327 Size: 107 122 71 631 605 577 675 1403 Cuts at: 4327 136 Size: 1525 Fragments arranged by size: 1525 1403 675 631 605 577 122 107 71 BglII A'GATC_T Cuts at: 5711 5711 Size: 5716 BlpI GC'TnA_GC Cuts at: 3133 3133 Size: 5716 Bme1580I G_kGCm'C Cuts at: 1790 2048 2063 2129 2188 2806 2875 3777 4933 Size: 258 15 66 59 618 69 902 1156 Cuts at: 4933 1790 Size: 2573 Fragments arranged by size: 2573 1156 902 618 258 69 66 59 15 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5716 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2100 2851 3447 Size: 416 53 83 399 24 683 751 596 Cuts at: 3447 4405 442 Size: 958 1753 Fragments arranged by size: 1753 958 751 683 596 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5716 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2079 2908 2079 Size: 829 4887 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4376 1239 Size: 3137 2579 Bpu10I CC'TnA_GC Cuts at: 2478 2478 Size: 5716 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3841 4706 5004 3841 Size: 865 298 4553 Fragments arranged by size: 4553 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4379 882 Size: 617 2880 2219 Fragments arranged by size: 2880 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3507 493 Size: 711 2303 2702 Fragments arranged by size: 2702 2303 711 BsaBI GATnn'nnATC Cuts at: 5710 5710 Size: 5716 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2211 3523 3905 4540 Size: 53 83 186 936 678 1312 382 635 Cuts at: 4540 275 Size: 1451 Fragments arranged by size: 1451 1312 936 678 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4149 4547 4821 4968 5379 4149 Size: 398 274 147 411 4486 Fragments arranged by size: 4486 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5491 Fragments arranged by size: 5491 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2275 2503 1186 Size: 1089 228 4399 Fragments arranged by size: 4399 1089 228 BseYI C'CCAG_C Cuts at: 2981 4919 5415 2981 Size: 1938 496 3282 Fragments arranged by size: 3282 1938 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5143 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3166 3927 4955 1540 Size: 275 186 1165 761 1028 2301 Fragments arranged by size: 2301 1165 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3777 3862 4933 3777 Size: 85 1071 4560 Fragments arranged by size: 4560 1071 85 BsmI GAATG_Cn' Cuts at: 3001 3227 3320 3001 Size: 226 93 5397 Fragments arranged by size: 5397 226 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2048 2063 2129 2188 2371 2806 2875 Size: 399 258 15 66 59 183 435 69 Cuts at: 2875 3035 3777 3862 4933 1391 Size: 160 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 435 399 258 183 160 85 69 66 59 15 BspEI T'CCGG_A Cuts at: 5379 5379 Size: 5716 BspHI T'CATG_A Cuts at: 3605 5335 3605 Size: 1730 3986 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3071 844 Size: 2227 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2902 3603 1394 Size: 429 1079 701 3507 Fragments arranged by size: 3507 1079 701 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4211 4385 65 Size: 1183 2963 174 1396 Fragments arranged by size: 2963 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2216 4360 4547 1700 Size: 110 406 2144 187 2869 Fragments arranged by size: 2869 2144 406 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5716 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5716 BssSI C'ACGA_G Cuts at: 3776 4788 3776 Size: 1012 4704 BstAPI GCAn_nnn'nTGC Cuts at: 794 2029 2504 2863 794 Size: 1235 475 359 3647 Fragments arranged by size: 3647 1235 475 359 BstBI TT'CG_AA Cuts at: 4520 4520 Size: 5716 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5716 BstYI r'GATC_y Cuts at: 1972 3046 3413 3798 3815 5267 5353 5365 5711 Size: 1074 367 385 17 1452 86 12 346 Cuts at: 5711 1972 Size: 1977 Fragments arranged by size: 1977 1452 1074 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2097 2244 2253 2344 513 Size: 552 939 93 147 9 91 3885 Fragments arranged by size: 3885 939 552 147 93 91 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4977 Fragments arranged by size: 4977 683 56 BtsI GCAGTG_nn' Cuts at: 2235 3232 4026 4046 2235 Size: 997 794 20 3905 Fragments arranged by size: 3905 997 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5716 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5681 DraI TTT'AAA Cuts at: 3020 3020 Size: 5716 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4723 809 Size: 507 3407 1802 Fragments arranged by size: 3407 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2290 3163 4052 Size: 54 1425 50 275 18 460 873 889 Cuts at: 4052 5681 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 873 460 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3163 1537 Size: 275 1351 4090 Fragments arranged by size: 4090 1351 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2564 3646 1353 Size: 1211 1082 3423 Fragments arranged by size: 3423 1211 1082 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4303 4677 4823 387 Size: 1064 138 2714 374 146 1280 Fragments arranged by size: 2714 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2300 3779 4376 5163 5431 1239 Size: 393 668 1479 597 787 268 1524 Fragments arranged by size: 1524 1479 787 668 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2126 2184 2185 2584 2803 2126 Size: 58 1 399 219 5039 Fragments arranged by size: 5039 399 219 58 1 EcoRI G'AATT_C Cuts at: 2321 2515 3025 3403 2321 Size: 194 510 378 4634 Fragments arranged by size: 4634 510 378 194 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2984 2984 Size: 5716 FspI TGC'GCA Cuts at: 4222 4222 Size: 5716 HaeII r_GCGC'y Cuts at: 1678 2075 2214 4863 1678 Size: 397 139 2649 2531 Fragments arranged by size: 2649 2531 397 139 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4357 4389 4431 4463 1373 Size: 32 135 32 2785 32 42 32 2626 Fragments arranged by size: 2785 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3060 3306 669 Size: 988 281 1122 246 3079 Fragments arranged by size: 3079 1122 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5716 HpaI GTT'AAC Cuts at: 3306 3306 Size: 5716 KasI G'GCGC_C Cuts at: 2210 2210 Size: 5716 KpnI G_GTAC'C Cuts at: 3041 3041 Size: 5716 MfeI C'AATT_G Cuts at: 3315 3315 Size: 5716 MluI A'CGCG_T Cuts at: 5592 5592 Size: 5716 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2421 2667 2691 3033 4830 5014 1305 Size: 1116 246 24 342 1797 184 2007 Fragments arranged by size: 2007 1797 1116 342 246 184 24 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3894 Fragments arranged by size: 3894 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2730 3674 4033 4192 518 Size: 648 1564 944 359 159 2042 Fragments arranged by size: 2042 1564 944 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2026 2052 2099 2170 3132 3809 4957 5202 Size: 420 26 47 71 962 677 1148 245 Cuts at: 5202 1606 Size: 2120 Fragments arranged by size: 2120 1148 962 677 420 245 71 47 26 NaeI GCC'GGC Cuts at: 2218 2218 Size: 5716 NarI GG'CG_CC Cuts at: 2211 2211 Size: 5716 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4225 Fragments arranged by size: 4225 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5716 NgoMIV G'CCGG_C Cuts at: 2216 2216 Size: 5716 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5716 NotI GC'GGCC_GC Cuts at: 3163 3163 Size: 5716 NsiI A_TGCA'T Cuts at: 3003 3003 Size: 5716 NspI r_CATG'y Cuts at: 1132 1229 3074 4619 1132 Size: 97 1845 1545 2229 Fragments arranged by size: 2229 1845 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5716 PciI A'CATG_T Cuts at: 4615 4615 Size: 5716 PflMI CCAn_nnn'nTGG Cuts at: 1108 2830 1108 Size: 1722 3994 PfoI T'CCnGG_A Cuts at: 2694 2694 Size: 5716 PmeI GTTT'AAAC Cuts at: 3020 3020 Size: 5716 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3785 3817 5298 5330 3785 Size: 32 1481 32 4171 Fragments arranged by size: 4171 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5716 PsiI TTA'TAA Cuts at: 3286 3286 Size: 5716 PspOMI G'GGCC_C Cuts at: 2125 2184 2802 2125 Size: 59 618 5039 Fragments arranged by size: 5039 618 59 PstI C_TGCA'G Cuts at: 830 2034 3068 830 Size: 1204 1034 3478 Fragments arranged by size: 3478 1204 1034 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5716 SacII CC_GC'GG Cuts at: 2100 2100 Size: 5716 SalI G'TCGA_C Cuts at: 1936 3058 1936 Size: 1122 4594 SbfI CC_TGCA'GG Cuts at: 3068 3068 Size: 5716 ScaI AGT'ACT Cuts at: 3964 5676 3964 Size: 1712 4004 SfcI C'TryA_G Cuts at: 826 1045 2030 3064 4199 4880 5071 826 Size: 219 985 1034 1135 681 191 1471 Fragments arranged by size: 1471 1135 1034 985 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2249 2249 Size: 5716 SfoI GGC'GCC Cuts at: 2212 2212 Size: 5716 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5716 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5716 SmaI CCC'GGG Cuts at: 1872 3043 1872 Size: 1171 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2741 3820 4721 4983 820 Size: 197 926 798 1079 901 262 1553 Fragments arranged by size: 1553 1079 926 901 798 262 197 SnaBI TAC'GTA Cuts at: 493 3507 493 Size: 3014 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5716 SphI G_CATG'C Cuts at: 1132 3074 1132 Size: 1942 3774 SspI AAT'ATT Cuts at: 5 52 3640 5 Size: 47 3588 2081 Fragments arranged by size: 3588 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5716 StyI C'CwwG_G Cuts at: 513 1065 2004 2835 3155 513 Size: 552 939 831 320 3074 Fragments arranged by size: 3074 939 831 552 320 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2833 3743 3760 3913 4098 4543 325 Size: 1255 1253 910 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1253 910 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3962 5674 96 Size: 274 80 33 51 1443 1985 1712 138 Fragments arranged by size: 1985 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5676 5708 5676 Size: 32 5684 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5716 XbaI T'CTAG_A Cuts at: 2879 3052 2879 Size: 173 5543 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5716 XmaI C'CCGG_G Cuts at: 1870 3041 1870 Size: 1171 4545 XmnI GAAnn'nnTTC Cuts at: 3845 3845 Size: 5716 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3524 276 Size: 53 83 186 936 1990 2468 Fragments arranged by size: 2468 1990 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SrfI SwaI XcmI