Restriction map for
pFN21AE0421
##################################
# NO CUT ENZYMES ( 169 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbsI BcgI
BciVI BclI BcnI BfrI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssKI BssSI Bst1107I
BstAPI BstBI BstEII BstNI BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRII EcoRV EcoT22I FauI
FbaI FseI FspI HaeII Hin1I
HindIII HpaI KasI KpnI Ksp632I
MamI MfeI MluI MluNI MroI
MscI MseI MunI MvaI NaeI
NarI NciI NdeI NgoMIV NheI
NlaIV NotI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SalI
SapI SbfI ScaI ScrFI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tru9I Tth111I TthHB8I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AfaI 378 GTAC GT^AC
AflIII 84 ACGTGT A^C[GA][CT]GT
Alw44I 285 GTGCAC G^TGCAC
ApaLI 285 GTGCAC G^TGCAC
AspHI 285 GTGCAC G[AT]GC[AT]^C
BbrPI 17 CACGTG CAC^GTG
BbvCI 484 GCTGAGG CCTCAGC(-5/-2)
BfaI 520 CTAG C^TAG
BglI 189 GCCATGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 285 GTGCAC G[AGT]GC[ACT]^C
BseRI 487 GAGGAG GAGGAG(10/8)
BsiHKAI 285 GTGCAC G[AT]GC[AT]^C
BsmAI 98 GAGAC GTCTC(1/5)
Bsp1286I 285 GTGCAC G[AGT]GC[ACT]^C
BssHII 49 GCGCGC G^CGCGC
BstXI 303 CCAGAAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 116 TGGCCG [CT]^GGCC[GA]
EcoT14I 190 CCATGG C^C[AT][AT]GG
HapII 23 CCGG C^CGG
HgaI 121 GCGTC GACGC(5/10)
HincII 388 GTTGAC GT[CT]^[GA]AC
HindII 388 GTTGAC GT[CT]^[GA]AC
HpaII 23 CCGG C^CGG
HphI 107 TCACC GGTGA(8/7)
KspI 59 CCGCGG CCGC^GG
MaeI 520 CTAG C^TAG
MslI 108 CACCGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 23 CCGG C^CGG
NcoI 190 CCATGG C^CATGG
NruI 94 TCGCGA TCG^CGA
PflMI 30 CCAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 154 GAGTC GAGTC(4/5)
PmaCI 17 CACGTG CAC^GTG
PmlI 17 CACGTG CAC^GTG
RsaI 378 GTAC GT^AC
SacII 59 CCGCGG CCGC^GG
SfaNI 562 GCATC GCATC(5/9)
StyI 190 CCATGG C^C[AT][AT]GG
TfiI 217 GAATC G^A[AT]TC
Van91I 30 CCAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 255 CCAGCTGTTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 147 AAATTT [GA]^AATT[CT]
172 GAATTT
AlwI 76 GATCC GGATC(4/5)
441 GATCC
ApoI 147 AAATTT [GA]^AATT[CT]
172 GAATTT
AvaII 162 GGACC G^G[AT]CC
223 GGACC
BsaAI 17 CACGTG [CT]AC^GT[GA]
83 TACGTG
BsaJI 59 CCGCGG C^C[ACGT][ACGT]GG
190 CCATGG
BstF5I 563 CATCC GGATG(2/0)
280 GGATG
BstYI 75 AGATCC [GA]^GATC[CT]
440 AGATCC
Cac8I 49 GCGCGC GC[ACGT]^[ACGT]GC
360 GCTTGC
Cfr13I 162 GGACC G^G[ACGT]CC
223 GGACC
DpnII 76 GATC ^GATC
441 GATC
DsaI 59 CCGCGG C^C[GA][CT]GG
190 CCATGG
FokI 563 CATCC GGATG(9/13)
280 GGATG
HaeIII 63 GGCC GG^CC
117 GGCC
HinfI 154 GAGTC G^A[ACGT]TC
217 GAATC
MaeII 18 ACGT A^CGT
84 ACGT
MboI 76 GATC ^GATC
441 GATC
MflI 75 AGATCC [GA]^GATC[CT]
440 AGATCC
NdeII 76 GATC ^GATC
441 GATC
Sau3AI 76 GATC ^GATC
441 GATC
Sau96I 162 GGACC G^G[ACGT]CC
223 GGACC
Tsp45I 156 GTCAC ^GT[GC]AC
274 GTCAC
Tsp509I 148 AATT ^AATT
173 AATT
TspRI 158 CACTG CA[GC]TG[ACGT][ACGT]^
276 CACTG
XhoII 75 AGATCC [GA]^GATC[CT]
440 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AccII AciI AluI BbvI BsiYI
BslI BsrI BstUI CfoI DdeI
Fnu4HI HhaI HinP1I ItaI MaeIII
MboII MnlI MspA1I MvnI MwoI
NlaIII PvuII TseI