(Circular) (Six-base) MAPSORT of: pFN21AE0421.seq Check: 4091 from: 1 to: 5272 pFN21AE0421 5272 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3082 278 Size: 53 83 186 936 1546 2468 Fragments arranged by size: 2468 1546 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2615 1937 Size: 678 4594 Acc65I G'GTAC_C Cuts at: 2593 2593 Size: 5272 AclI AA'CG_TT Cuts at: 1563 3399 3772 1563 Size: 1836 373 3063 Fragments arranged by size: 3063 1836 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2217 2345 2462 3335 4719 4987 1632 Size: 585 128 117 873 1384 268 1917 Fragments arranged by size: 1917 1384 873 585 268 128 117 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5075 AflIII A'CryG_T Cuts at: 1203 2089 4171 5148 1203 Size: 886 2082 977 1327 Fragments arranged by size: 2082 1327 977 886 AgeI A'CCGG_T Cuts at: 4103 4103 Size: 5272 AhdI GACnn_n'nnGTC Cuts at: 4001 4001 Size: 5272 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5272 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5272 AlwNI CAG_nnn'CTG Cuts at: 3015 4587 3015 Size: 1572 3700 ApaLI G'TGCA_C Cuts at: 2290 3329 4485 2290 Size: 1039 1156 3077 Fragments arranged by size: 3077 1156 1039 ApoI r'AATT_y Cuts at: 1493 2152 2177 2581 2807 2959 5068 1493 Size: 659 25 404 226 152 2109 1697 Fragments arranged by size: 2109 1697 659 404 226 152 25 AseI AT'TA_AT Cuts at: 160 1032 3826 160 Size: 872 2794 1606 Fragments arranged by size: 2794 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5272 AvaI C'yCGr_G Cuts at: 1870 1943 2597 1870 Size: 73 654 4545 Fragments arranged by size: 4545 654 73 BamHI G'GATC_C Cuts at: 2602 2969 2602 Size: 367 4905 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2593 4048 618 Size: 325 217 627 806 1455 1842 Fragments arranged by size: 1842 1455 806 627 325 217 BanII G_rGCy'C Cuts at: 1391 2591 1391 Size: 1200 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4301 BbvCI CC'TCA_GC Cuts at: 2490 2490 Size: 5272 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3463 3497 1903 Size: 34 1526 34 3678 Fragments arranged by size: 3678 1526 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3164 4374 3164 Size: 1210 4062 BclI T'GATC_A Cuts at: 1548 1548 Size: 5272 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2200 3883 136 Size: 107 122 71 631 605 528 1683 1525 Fragments arranged by size: 1683 1525 631 605 528 122 107 71 BglII A'GATC_T Cuts at: 5267 5267 Size: 5272 BlpI GC'TnA_GC Cuts at: 2689 2689 Size: 5272 Bme1580I G_kGCm'C Cuts at: 1790 2294 3333 4489 1790 Size: 504 1039 1156 2573 Fragments arranged by size: 2573 1156 1039 504 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5272 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2388 2541 3003 Size: 416 53 83 399 24 971 153 462 Cuts at: 3003 3961 442 Size: 958 1753 Fragments arranged by size: 1753 971 958 462 416 399 153 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5272 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2126 2126 Size: 5272 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3932 1239 Size: 2693 2579 Bpu10I CC'TnA_GC Cuts at: 2490 2490 Size: 5272 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3397 4262 4560 3397 Size: 865 298 4109 Fragments arranged by size: 4109 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3935 882 Size: 617 2436 2219 Fragments arranged by size: 2436 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2024 2090 3063 493 Size: 711 820 66 973 2702 Fragments arranged by size: 2702 973 820 711 66 BsaBI GATnn'nnATC Cuts at: 5266 5266 Size: 5272 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3079 3461 4096 275 Size: 53 83 186 936 1546 382 635 1451 Fragments arranged by size: 1546 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3705 4103 4377 4524 4935 3705 Size: 398 274 147 411 4042 Fragments arranged by size: 4042 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2486 2516 1178 Size: 30 165 30 1083 30 3934 Fragments arranged by size: 3934 1083 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2507 1186 Size: 1321 3951 BseYI C'CCAG_C Cuts at: 2259 4475 4971 2259 Size: 2216 496 2560 Fragments arranged by size: 2560 2216 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4699 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2722 3483 4511 1540 Size: 275 186 721 761 1028 2301 Fragments arranged by size: 2301 1028 761 721 275 186 BsiHKAI G_wGCw'C Cuts at: 2294 3333 3418 4489 2294 Size: 1039 85 1071 3077 Fragments arranged by size: 3077 1071 1039 85 BsmI GAATG_Cn' Cuts at: 2783 2876 2783 Size: 93 5179 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2294 2591 3333 3418 4489 1391 Size: 399 504 297 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 504 399 297 85 BspEI T'CCGG_A Cuts at: 4935 4935 Size: 5272 BspHI T'CATG_A Cuts at: 3161 4891 3161 Size: 1730 3542 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2627 844 Size: 1783 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3159 1394 Size: 429 1336 3507 Fragments arranged by size: 3507 1336 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3767 3941 65 Size: 1183 2519 174 1396 Fragments arranged by size: 2519 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3916 4103 1700 Size: 110 2106 187 2869 Fragments arranged by size: 2869 2106 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5272 BssHII G'CGCG_C Cuts at: 1925 2054 1925 Size: 129 5143 BssSI C'ACGA_G Cuts at: 3332 4344 3332 Size: 1012 4260 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5272 BstBI TT'CG_AA Cuts at: 4076 4076 Size: 5272 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5272 BstXI CCAn_nnnn'nTGG Cuts at: 2315 2315 Size: 5272 BstYI r'GATC_y Cuts at: 1972 2080 2445 2602 2969 3354 3371 4823 4909 Size: 108 365 157 367 385 17 1452 86 Cuts at: 4909 4921 5267 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 365 346 157 108 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2064 2195 513 Size: 552 939 60 131 3590 Fragments arranged by size: 3590 939 552 131 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4533 Fragments arranged by size: 4533 683 56 BtsI GCAGTG_nn' Cuts at: 2788 3582 3602 2788 Size: 794 20 4458 Fragments arranged by size: 4458 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5272 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2319 2354 529 Size: 35 1755 35 3447 Fragments arranged by size: 3447 1755 35 35 DraI TTT'AAA Cuts at: 2576 2576 Size: 5272 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4279 809 Size: 507 2963 1802 Fragments arranged by size: 2963 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2121 2719 3608 Size: 54 1425 50 275 18 291 598 889 Cuts at: 3608 5237 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 598 291 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2719 1537 Size: 275 907 4090 Fragments arranged by size: 4090 907 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3202 1353 Size: 1849 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3859 4233 4379 387 Size: 1064 138 2270 374 146 1280 Fragments arranged by size: 2270 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2217 2345 2462 3335 3932 4719 4987 Size: 393 585 128 117 873 597 787 268 Cuts at: 4987 1239 Size: 1524 Fragments arranged by size: 1524 873 787 597 585 393 268 128 117 EcoRI G'AATT_C Cuts at: 2581 2959 2581 Size: 378 4894 FspI TGC'GCA Cuts at: 3778 3778 Size: 5272 HaeII r_GCGC'y Cuts at: 1678 4419 1678 Size: 2741 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2340 2372 2486 2518 3913 Size: 32 135 32 768 32 114 32 1395 Cuts at: 3913 3945 3987 4019 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1395 768 135 114 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2395 2616 2862 669 Size: 988 281 457 221 246 3079 Fragments arranged by size: 3079 988 457 281 246 221 HindIII A'AGCT_T Cuts at: 748 748 Size: 5272 HpaI GTT'AAC Cuts at: 2862 2862 Size: 5272 KpnI G_GTAC'C Cuts at: 2597 2597 Size: 5272 MfeI C'AATT_G Cuts at: 2871 2871 Size: 5272 MluI A'CGCG_T Cuts at: 5148 5148 Size: 5272 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2296 4386 4570 1305 Size: 991 2090 184 2007 Fragments arranged by size: 2090 2007 991 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3450 Fragments arranged by size: 3450 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2117 3230 3589 3748 518 Size: 648 951 1113 359 159 2042 Fragments arranged by size: 2042 1113 951 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2066 2263 2336 2440 2688 3365 4513 4758 Size: 460 197 73 104 248 677 1148 245 Cuts at: 4758 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 460 248 245 197 104 73 NcoI C'CATG_G Cuts at: 513 1065 2004 2195 513 Size: 552 939 191 3590 Fragments arranged by size: 3590 939 552 191 NdeI CA'TA_TG Cuts at: 387 387 Size: 5272 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5272 NotI GC'GGCC_GC Cuts at: 2719 2719 Size: 5272 NruI TCG'CGA Cuts at: 2101 2101 Size: 5272 NspI r_CATG'y Cuts at: 1132 1229 2630 4175 1132 Size: 97 1401 1545 2229 Fragments arranged by size: 2229 1545 1401 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5272 PciI A'CATG_T Cuts at: 4171 4171 Size: 5272 PflMI CCAn_nnn'nTGG Cuts at: 1108 2041 1108 Size: 933 4339 PmeI GTTT'AAAC Cuts at: 2576 2576 Size: 5272 PmlI CAC'GTG Cuts at: 2024 2024 Size: 5272 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3341 3373 4854 4886 3341 Size: 32 1481 32 3727 Fragments arranged by size: 3727 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5272 PsiI TTA'TAA Cuts at: 2842 2842 Size: 5272 PstI C_TGCA'G Cuts at: 830 2624 830 Size: 1794 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5272 PvuII CAG'CTG Cuts at: 2263 2336 2440 2263 Size: 73 104 5095 Fragments arranged by size: 5095 104 73 SacII CC_GC'GG Cuts at: 2067 2067 Size: 5272 SalI G'TCGA_C Cuts at: 1936 2614 1936 Size: 678 4594 SbfI CC_TGCA'GG Cuts at: 2624 2624 Size: 5272 ScaI AGT'ACT Cuts at: 3520 5232 3520 Size: 1712 3560 SfcI C'TryA_G Cuts at: 826 1045 2620 3755 4436 4627 826 Size: 219 1575 1135 681 191 1471 Fragments arranged by size: 1575 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5272 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5272 SmaI CCC'GGG Cuts at: 1872 2599 1872 Size: 727 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3376 4277 4539 820 Size: 197 926 1433 901 262 1553 Fragments arranged by size: 1553 1433 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3063 493 Size: 2570 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5272 SphI G_CATG'C Cuts at: 1132 2630 1132 Size: 1498 3774 SspI AAT'ATT Cuts at: 5 52 3196 5 Size: 47 3144 2081 Fragments arranged by size: 3144 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5272 StyI C'CwwG_G Cuts at: 513 1065 2004 2195 2711 513 Size: 552 939 191 516 3074 Fragments arranged by size: 3074 939 552 516 191 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3299 3316 3469 3654 4099 325 Size: 1255 1719 17 153 185 445 1498 Fragments arranged by size: 1719 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3518 5230 96 Size: 274 80 33 51 1443 1541 1712 138 Fragments arranged by size: 1712 1541 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2022 2054 5232 5264 2022 Size: 32 3178 32 2030 Fragments arranged by size: 3178 2030 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5272 XbaI T'CTAG_A Cuts at: 2608 2608 Size: 5272 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2267 2267 Size: 5272 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5272 XmaI C'CCGG_G Cuts at: 1870 2597 1870 Size: 727 4545 XmnI GAAnn'nnTTC Cuts at: 3401 3401 Size: 5272 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3080 276 Size: 53 83 186 936 1546 2468 Fragments arranged by size: 2468 1546 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI