(Circular) (Six-base) MAPSORT of: pFN21AE0341.seq Check: 2813 from: 1 to: 6757 pFN21AE0341 6757 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3534 4567 278 Size: 53 83 186 936 1998 1033 2468 Fragments arranged by size: 2468 1998 1033 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4100 1937 Size: 2163 4594 Acc65I G'GTAC_C Cuts at: 3858 4078 3858 Size: 220 6537 AclI AA'CG_TT Cuts at: 1563 4884 5257 1563 Size: 3321 373 3063 Fragments arranged by size: 3321 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2320 2891 4820 6204 6472 1632 Size: 688 571 1929 1384 268 1917 Fragments arranged by size: 1929 1917 1384 688 571 268 AfeI AGC'GCT Cuts at: 2076 2076 Size: 6757 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6560 AflIII A'CryG_T Cuts at: 1203 5656 6633 1203 Size: 4453 977 1327 Fragments arranged by size: 4453 1327 977 AgeI A'CCGG_T Cuts at: 3546 5588 3546 Size: 2042 4715 AhdI GACnn_n'nnGTC Cuts at: 5486 5486 Size: 6757 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6757 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3820 3941 1184 Size: 2636 121 4000 Fragments arranged by size: 4000 2636 121 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3626 3658 3626 Size: 32 6725 AlwNI CAG_nnn'CTG Cuts at: 3433 4500 6072 3433 Size: 1067 1572 4118 Fragments arranged by size: 4118 1572 1067 ApaI G_GGCC'C Cuts at: 2124 2191 2124 Size: 67 6690 ApaLI G'TGCA_C Cuts at: 4814 5970 4814 Size: 1156 5601 ApoI r'AATT_y Cuts at: 1493 2906 3388 3508 4066 4292 4444 6553 1493 Size: 1413 482 120 558 226 152 2109 1697 Fragments arranged by size: 2109 1697 1413 558 482 226 152 120 AseI AT'TA_AT Cuts at: 160 1032 5311 160 Size: 872 4279 1606 Fragments arranged by size: 4279 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6757 AvaI C'yCGr_G Cuts at: 1870 1943 2041 2739 2840 3070 4082 1870 Size: 73 98 698 101 230 1012 4545 Fragments arranged by size: 4545 1012 698 230 101 98 73 BamHI G'GATC_C Cuts at: 4087 4454 4087 Size: 367 6390 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2083 2226 3858 3951 4078 Size: 325 217 627 296 143 1632 93 127 Cuts at: 4078 5533 618 Size: 1455 1842 Fragments arranged by size: 1842 1632 1455 627 325 296 217 143 127 93 BanII G_rGCy'C Cuts at: 1391 2124 2191 2418 3285 3342 3480 4056 4076 Size: 733 67 227 867 57 138 576 20 Cuts at: 4076 1391 Size: 4072 Fragments arranged by size: 4072 867 733 576 227 138 67 57 20 BbeI G_GCGC'C Cuts at: 3955 3955 Size: 6757 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3526 3705 928 Size: 971 1627 179 3980 Fragments arranged by size: 3980 1627 971 179 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4948 4982 1903 Size: 34 3011 34 3678 Fragments arranged by size: 3678 3011 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2800 4649 5859 2800 Size: 1849 1210 3698 Fragments arranged by size: 3698 1849 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6757 BfrBI ATG'CAT Cuts at: 3080 3080 Size: 6757 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5368 136 Size: 107 122 71 631 605 3696 1525 Fragments arranged by size: 3696 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6752 6752 Size: 6757 BlpI GC'TnA_GC Cuts at: 2312 2729 4174 2312 Size: 417 1445 4895 Fragments arranged by size: 4895 1445 417 Bme1580I G_kGCm'C Cuts at: 1790 2088 2124 2191 2997 3561 3735 4818 5974 Size: 298 36 67 806 564 174 1083 1156 Cuts at: 5974 1790 Size: 2573 Fragments arranged by size: 2573 1156 1083 806 564 298 174 67 36 BmgBI CAC'GTC Cuts at: 1318 2449 1318 Size: 1131 5626 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4488 5446 442 Size: 416 53 83 399 24 3071 958 1753 Fragments arranged by size: 3071 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3114 1056 Size: 2058 4699 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3263 3431 3464 3779 3977 3996 5417 1239 Size: 2024 168 33 315 198 19 1421 2579 Fragments arranged by size: 2579 2024 1421 315 198 168 33 19 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2804 4882 5747 6045 2804 Size: 2078 865 298 3516 Fragments arranged by size: 3516 2078 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2769 5420 882 Size: 617 1270 2651 2219 Fragments arranged by size: 2651 2219 1270 617 BsaAI yAC'GTr Cuts at: 493 1204 4548 493 Size: 711 3344 2702 Fragments arranged by size: 3344 2702 711 BsaBI GATnn'nnATC Cuts at: 6751 6751 Size: 6757 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2367 3531 3952 4564 Size: 53 83 186 936 834 1164 421 612 Cuts at: 4564 4946 5581 275 Size: 382 635 1451 Fragments arranged by size: 1451 1164 936 834 635 612 421 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3177 3546 5190 5588 5862 6009 6420 3177 Size: 369 1644 398 274 147 411 3514 Fragments arranged by size: 3514 1644 411 398 369 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3626 3656 1178 Size: 30 165 30 2223 30 4279 Fragments arranged by size: 4279 2223 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3428 4064 1186 Size: 2242 636 3879 Fragments arranged by size: 3879 2242 636 BseYI C'CCAG_C Cuts at: 2071 2110 2117 2410 3188 3438 5960 6456 2071 Size: 39 7 293 778 250 2522 496 2372 Fragments arranged by size: 2522 2372 778 496 293 250 39 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2051 2548 3636 1163 Size: 573 315 497 1088 4284 Fragments arranged by size: 4284 1088 573 497 315 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3729 4207 4968 5996 1540 Size: 275 186 1728 478 761 1028 2301 Fragments arranged by size: 2301 1728 1028 761 478 275 186 BsiHKAI G_wGCw'C Cuts at: 3249 3285 4056 4818 4903 5974 3249 Size: 36 771 762 85 1071 4032 Fragments arranged by size: 4032 1071 771 762 85 36 BsmI GAATG_Cn' Cuts at: 3080 4268 4361 3080 Size: 1188 93 5476 Fragments arranged by size: 5476 1188 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2088 2124 2191 2418 2997 3249 3285 Size: 399 298 36 67 227 579 252 36 Cuts at: 3285 3342 3480 3561 3735 4056 4076 4818 4903 Size: 57 138 81 174 321 20 742 85 Cuts at: 4903 5974 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 579 399 321 298 252 227 174 138 85 81 67 57 36 36 20 BspEI T'CCGG_A Cuts at: 6420 6420 Size: 6757 BspHI T'CATG_A Cuts at: 4646 6376 4646 Size: 1730 5027 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4112 844 Size: 3268 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2185 2736 4644 1394 Size: 429 362 551 1908 3507 Fragments arranged by size: 3507 1908 551 429 362 BsrDI GCAATG_nn' Cuts at: 65 1248 5252 5426 65 Size: 1183 4004 174 1396 Fragments arranged by size: 4004 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3234 3546 5401 5588 1700 Size: 110 1424 312 1855 187 2869 Fragments arranged by size: 2869 1855 1424 312 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6757 BssHII G'CGCG_C Cuts at: 1925 2155 1925 Size: 230 6527 BssSI C'ACGA_G Cuts at: 3878 4817 5829 3878 Size: 939 1012 4806 Fragments arranged by size: 4806 1012 939 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6757 BstBI TT'CG_AA Cuts at: 5561 5561 Size: 6757 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6757 BstXI CCAn_nnnn'nTGG Cuts at: 2771 3977 2771 Size: 1206 5551 BstYI r'GATC_y Cuts at: 1972 2857 4087 4454 4839 4856 6308 6394 6406 Size: 885 1230 367 385 17 1452 86 12 Cuts at: 6406 6752 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1230 885 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2628 3863 2628 Size: 1235 5522 BtgI C'CryG_G Cuts at: 513 1065 2004 2124 2192 2222 2619 3202 3393 Size: 552 939 120 68 30 397 583 191 Cuts at: 3393 3675 513 Size: 282 3595 Fragments arranged by size: 3595 939 583 552 397 282 191 120 68 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2044 2425 487 Size: 683 56 818 381 4819 Fragments arranged by size: 4819 818 683 381 56 BtsI GCAGTG_nn' Cuts at: 2181 4273 5067 5087 2181 Size: 2092 794 20 3851 Fragments arranged by size: 3851 2092 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6757 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3382 3417 529 Size: 35 2818 35 3869 Fragments arranged by size: 3869 2818 35 35 DraI TTT'AAA Cuts at: 4061 4061 Size: 6757 DraIII CAC_nnn'GTG Cuts at: 3169 3694 3910 3169 Size: 525 216 6016 Fragments arranged by size: 6016 525 216 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3530 5764 809 Size: 507 2214 2234 1802 Fragments arranged by size: 2234 2214 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2482 2862 Size: 54 1425 50 275 18 225 427 380 Cuts at: 2862 3363 3678 4204 5093 6722 8 Size: 501 315 526 889 1629 43 Fragments arranged by size: 1629 1425 889 526 501 427 380 315 275 225 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4204 1537 Size: 275 2392 4090 Fragments arranged by size: 4090 2392 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2339 3485 4687 1353 Size: 986 1146 1202 3423 Fragments arranged by size: 3423 1202 1146 986 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2279 3635 5344 5718 5864 387 Size: 1064 138 690 1356 1709 374 146 1280 Fragments arranged by size: 1709 1356 1280 1064 690 374 146 138 EcoICRI GAG'CTC Cuts at: 3283 4054 3283 Size: 771 5986 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2320 2891 3263 3431 3464 3779 3977 Size: 393 688 571 372 168 33 315 198 Cuts at: 3977 3996 4820 5417 6204 6472 1239 Size: 19 824 597 787 268 1524 Fragments arranged by size: 1524 824 787 688 597 571 393 372 315 268 198 168 33 19 EcoNI CCTnn'n_nnAGG Cuts at: 2960 3380 2960 Size: 420 6337 EcoO109I rG'GnC_Cy Cuts at: 2036 2188 4016 2036 Size: 152 1828 4777 Fragments arranged by size: 4777 1828 152 EcoRI G'AATT_C Cuts at: 3388 4066 4444 3388 Size: 678 378 5701 Fragments arranged by size: 5701 678 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3550 3550 Size: 6757 FspI TGC'GCA Cuts at: 5263 5263 Size: 6757 HaeII r_GCGC'y Cuts at: 1678 2078 2118 3754 3955 5904 1678 Size: 400 40 1636 201 1949 2531 Fragments arranged by size: 2531 1949 1636 400 201 40 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2288 2320 2600 2632 2647 Size: 32 135 32 716 32 280 32 15 Cuts at: 2647 2679 3533 3565 5398 5430 5472 5504 1373 Size: 32 854 32 1833 32 42 32 2626 Fragments arranged by size: 2626 1833 854 716 280 135 42 32 32 32 32 32 32 32 32 15 HincII GTy'rAC Cuts at: 669 1657 1938 2575 4101 4347 669 Size: 988 281 637 1526 246 3079 Fragments arranged by size: 3079 1526 988 637 281 246 HindIII A'AGCT_T Cuts at: 748 3787 748 Size: 3039 3718 HpaI GTT'AAC Cuts at: 4347 4347 Size: 6757 KasI G'GCGC_C Cuts at: 3951 3951 Size: 6757 KpnI G_GTAC'C Cuts at: 3862 4082 3862 Size: 220 6537 MfeI C'AATT_G Cuts at: 4356 4356 Size: 6757 MluI A'CGCG_T Cuts at: 6633 6633 Size: 6757 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2752 5871 6055 1305 Size: 1447 3119 184 2007 Fragments arranged by size: 3119 2007 1447 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2057 2484 2864 10 Size: 54 1425 343 225 427 380 3903 Fragments arranged by size: 3903 1425 427 380 343 225 54 MslI CAynn'nnrTG Cuts at: 518 1166 2990 3428 3491 4715 5074 5233 518 Size: 648 1824 438 63 1224 359 159 2042 Fragments arranged by size: 2042 1824 1224 648 438 359 159 63 MspA1I CmG'CkG Cuts at: 1606 2018 2126 2194 2224 3015 3100 3298 3442 Size: 412 108 68 30 791 85 198 144 Cuts at: 3442 4173 4850 5998 6243 1606 Size: 731 677 1148 245 2120 Fragments arranged by size: 2120 1148 791 731 677 412 245 198 144 108 85 68 30 NarI GG'CG_CC Cuts at: 3952 3952 Size: 6757 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5266 Fragments arranged by size: 5266 939 552 NdeI CA'TA_TG Cuts at: 387 2724 387 Size: 2337 4420 NheI G'CTAG_C Cuts at: 1052 3110 1052 Size: 2058 4699 NotI GC'GGCC_GC Cuts at: 4204 4204 Size: 6757 NsiI A_TGCA'T Cuts at: 3082 3082 Size: 6757 NspI r_CATG'y Cuts at: 1132 1229 4115 5660 1132 Size: 97 2886 1545 2229 Fragments arranged by size: 2886 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6757 PciI A'CATG_T Cuts at: 5656 5656 Size: 6757 PflMI CCAn_nnn'nTGG Cuts at: 1108 3169 1108 Size: 2061 4696 PfoI T'CCnGG_A Cuts at: 2913 3240 3756 2913 Size: 327 516 5914 Fragments arranged by size: 5914 516 327 PmeI GTTT'AAAC Cuts at: 4061 4061 Size: 6757 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3626 3658 4826 4858 6339 6371 3626 Size: 32 1168 32 1481 32 4012 Fragments arranged by size: 4012 1481 1168 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2036 2036 Size: 6757 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6757 PsiI TTA'TAA Cuts at: 4327 4327 Size: 6757 PspOMI G'GGCC_C Cuts at: 2120 2187 2120 Size: 67 6690 PspXI vC'TCGA_Gb Cuts at: 2840 2840 Size: 6757 PstI C_TGCA'G Cuts at: 830 2136 2154 4109 830 Size: 1306 18 1955 3478 Fragments arranged by size: 3478 1955 1306 18 PvuI CG_AT'CG Cuts at: 2001 3729 2001 Size: 1728 5029 PvuII CAG'CTG Cuts at: 3298 3442 3298 Size: 144 6613 SacI G_AGCT'C Cuts at: 3285 4056 3285 Size: 771 5986 SacII CC_GC'GG Cuts at: 2127 2195 2225 2127 Size: 68 30 6659 Fragments arranged by size: 6659 68 30 SalI G'TCGA_C Cuts at: 1936 4099 1936 Size: 2163 4594 SanDI GG'GwC_CC Cuts at: 2036 2036 Size: 6757 SapI GCTCTTCn'nnn_ Cuts at: 2339 3485 2339 Size: 1146 5611 SbfI CC_TGCA'GG Cuts at: 4109 4109 Size: 6757 ScaI AGT'ACT Cuts at: 5005 6717 5005 Size: 1712 5045 SexAI A'CCwGG_T Cuts at: 2568 2568 Size: 6757 SfcI C'TryA_G Cuts at: 826 1045 2132 2150 3610 4105 5240 5921 6112 Size: 219 1087 18 1460 495 1135 681 191 Cuts at: 6112 826 Size: 1471 Fragments arranged by size: 1471 1460 1135 1087 681 495 219 191 18 SfoI GGC'GCC Cuts at: 3953 3953 Size: 6757 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6757 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6757 SmaI CCC'GGG Cuts at: 1872 2043 2741 4084 1872 Size: 171 698 1343 4545 Fragments arranged by size: 4545 1343 698 171 SmlI C'TyrA_G Cuts at: 820 1017 1943 2819 2840 4861 5762 6024 820 Size: 197 926 876 21 2021 901 262 1553 Fragments arranged by size: 2021 1553 926 901 876 262 197 21 SnaBI TAC'GTA Cuts at: 493 4548 493 Size: 4055 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6757 SphI G_CATG'C Cuts at: 1132 4115 1132 Size: 2983 3774 SspI AAT'ATT Cuts at: 5 52 4681 5 Size: 47 4629 2081 Fragments arranged by size: 4629 2081 47 StuI AGG'CCT Cuts at: 1517 2474 1517 Size: 957 5800 StyI C'CwwG_G Cuts at: 513 1065 2004 4196 513 Size: 552 939 2192 3074 Fragments arranged by size: 3074 2192 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2085 2381 2481 2693 2777 4784 4801 Size: 1255 505 296 100 212 84 2007 17 Cuts at: 4801 4954 5139 5584 325 Size: 153 185 445 1498 Fragments arranged by size: 2007 1498 1255 505 445 296 212 185 153 100 84 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2427 5003 6715 Size: 274 80 33 51 1443 450 2576 1712 Cuts at: 6715 96 Size: 138 Fragments arranged by size: 2576 1712 1443 450 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6717 6749 6717 Size: 32 6725 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6757 XbaI T'CTAG_A Cuts at: 4093 4093 Size: 6757 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3166 3430 3166 Size: 264 6493 XhoI C'TCGA_G Cuts at: 1943 2840 1943 Size: 897 5860 XmaI C'CCGG_G Cuts at: 1870 2041 2739 4082 1870 Size: 171 698 1343 4545 Fragments arranged by size: 4545 1343 698 171 XmnI GAAnn'nnTTC Cuts at: 2909 4886 2909 Size: 1977 4780 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3532 4565 276 Size: 53 83 186 936 1998 1033 2468 Fragments arranged by size: 2468 1998 1033 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PsrI RsrII SfiI SrfI SwaI