(Circular) (Six-base) MAPSORT of: pFN21AB6165.seq Check: 5472 from: 1 to: 6649 pFN21AB6165 6649 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3776 3776 Size: 6649 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2037 2406 4459 278 Size: 53 83 186 936 501 369 2053 2468 Fragments arranged by size: 2468 2053 936 501 369 186 83 53 AccI GT'mk_AC Cuts at: 1937 3992 1937 Size: 2055 4594 Acc65I G'GTAC_C Cuts at: 3970 3970 Size: 6649 AclI AA'CG_TT Cuts at: 1563 4776 5149 1563 Size: 3213 373 3063 Fragments arranged by size: 3213 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2125 2260 4712 6096 6364 1632 Size: 493 135 2452 1384 268 1917 Fragments arranged by size: 2452 1917 1384 493 268 135 AflII C'TTAA_G Cuts at: 820 1017 2855 820 Size: 197 1838 4614 Fragments arranged by size: 4614 1838 197 AflIII A'CryG_T Cuts at: 1203 5548 6525 1203 Size: 4345 977 1327 Fragments arranged by size: 4345 1327 977 AgeI A'CCGG_T Cuts at: 5480 5480 Size: 6649 AhdI GACnn_n'nnGTC Cuts at: 5378 5378 Size: 6649 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6649 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6649 AlwNI CAG_nnn'CTG Cuts at: 4392 5964 4392 Size: 1572 5077 ApaI G_GGCC'C Cuts at: 2051 2229 2274 3046 3454 3759 2051 Size: 178 45 772 408 305 4941 Fragments arranged by size: 4941 772 408 305 178 45 ApaLI G'TGCA_C Cuts at: 4706 5862 4706 Size: 1156 5493 ApoI r'AATT_y Cuts at: 1493 2953 3958 4184 4336 6445 1493 Size: 1460 1005 226 152 2109 1697 Fragments arranged by size: 2109 1697 1460 1005 226 152 AseI AT'TA_AT Cuts at: 160 1032 5203 160 Size: 872 4171 1606 Fragments arranged by size: 4171 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6649 AvaI C'yCGr_G Cuts at: 1870 1943 2228 3802 3974 1870 Size: 73 285 1574 172 4545 Fragments arranged by size: 4545 1574 285 172 73 BamHI G'GATC_C Cuts at: 3979 4346 3979 Size: 367 6282 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2092 2773 2796 3244 3435 Size: 325 217 627 305 681 23 448 191 Cuts at: 3435 3461 3970 5425 618 Size: 26 509 1455 1842 Fragments arranged by size: 1842 1455 681 627 509 448 325 305 217 191 26 23 BanII G_rGCy'C Cuts at: 1391 2051 2229 2274 2556 2747 3046 3185 3454 Size: 660 178 45 282 191 299 139 269 Cuts at: 3454 3759 3879 3968 1391 Size: 305 120 89 4072 Fragments arranged by size: 4072 660 305 299 282 269 191 178 139 120 89 45 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2398 928 Size: 971 499 5179 Fragments arranged by size: 5179 971 499 BbvCI CC'TCA_GC Cuts at: 3917 3917 Size: 6649 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4840 4874 1903 Size: 34 2903 34 3678 Fragments arranged by size: 3678 2903 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2310 4541 5751 2310 Size: 2231 1210 3208 Fragments arranged by size: 3208 2231 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6649 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2106 2256 2278 Size: 107 122 71 631 605 434 150 22 Cuts at: 2278 3211 3433 5260 136 Size: 933 222 1827 1525 Fragments arranged by size: 1827 1525 933 631 605 434 222 150 122 107 71 22 BglII A'GATC_T Cuts at: 2892 6644 2892 Size: 3752 2897 BlpI GC'TnA_GC Cuts at: 4066 4066 Size: 6649 Bme1580I G_kGCm'C Cuts at: 1790 2051 2229 2274 2799 3046 3342 3438 3454 Size: 261 178 45 525 247 296 96 16 Cuts at: 3454 3759 3826 4710 5866 1790 Size: 305 67 884 1156 2573 Fragments arranged by size: 2573 1156 884 525 305 296 261 247 178 96 67 45 16 BmgBI CAC'GTC Cuts at: 1318 2497 1318 Size: 1179 5470 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2483 2612 2880 Size: 416 53 83 399 24 1066 129 268 Cuts at: 2880 3836 4380 5338 442 Size: 956 544 958 1753 Fragments arranged by size: 1753 1066 958 956 544 416 399 268 129 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6649 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2081 2157 2489 2645 3276 3893 5309 1239 Size: 842 76 332 156 631 617 1416 2579 Fragments arranged by size: 2579 1416 842 631 617 332 156 76 Bpu10I CC'TnA_GC Cuts at: 3223 3917 3223 Size: 694 5955 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2336 3461 4774 5639 5937 2336 Size: 1125 1313 865 298 3048 Fragments arranged by size: 3048 1313 1125 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2414 3567 5312 882 Size: 617 915 1153 1745 2219 Fragments arranged by size: 2219 1745 1153 915 617 BsaAI yAC'GTr Cuts at: 493 1204 4440 493 Size: 711 3236 2702 Fragments arranged by size: 3236 2702 711 BsaBI GATnn'nnATC Cuts at: 6643 6643 Size: 6649 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2034 2403 4456 4838 Size: 53 83 186 936 501 369 2053 382 Cuts at: 4838 5473 275 Size: 635 1451 Fragments arranged by size: 2053 1451 936 635 501 382 369 186 83 53 BsaWI w'CCGG_w Cuts at: 5082 5480 5754 5901 6312 5082 Size: 398 274 147 411 5419 Fragments arranged by size: 5419 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2085 2115 2666 2696 2729 Size: 30 165 30 682 30 551 30 33 Cuts at: 2729 2759 3332 3362 1178 Size: 30 573 30 4465 Fragments arranged by size: 4465 682 573 551 165 33 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2333 2495 2671 2737 3092 1186 Size: 1147 162 176 66 355 4743 Fragments arranged by size: 4743 1147 355 176 162 66 BseYI C'CCAG_C Cuts at: 2251 2564 2636 2685 3178 3250 3286 3453 3500 Size: 313 72 49 493 72 36 167 47 Cuts at: 3500 3535 3726 3923 5852 6348 2251 Size: 35 191 197 1929 496 2552 Fragments arranged by size: 2552 1929 496 493 313 197 191 167 72 72 49 47 36 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3257 3821 1163 Size: 573 1521 564 3991 Fragments arranged by size: 3991 1521 573 564 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4099 4860 5888 1540 Size: 275 186 2098 761 1028 2301 Fragments arranged by size: 2301 2098 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2916 4710 4795 5866 2916 Size: 1794 85 1071 3699 Fragments arranged by size: 3699 1794 1071 85 BsmI GAATG_Cn' Cuts at: 4160 4253 4160 Size: 93 6556 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2051 2229 2274 2556 2747 2799 2916 Size: 399 261 178 45 282 191 52 117 Cuts at: 2916 3046 3185 3342 3438 3454 3759 3826 3879 Size: 130 139 157 96 16 305 67 53 Cuts at: 3879 3968 4710 4795 5866 1391 Size: 89 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 305 282 261 191 178 157 139 130 117 96 89 85 67 53 52 45 16 BspEI T'CCGG_A Cuts at: 6312 6312 Size: 6649 BspHI T'CATG_A Cuts at: 4538 6268 4538 Size: 1730 4919 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3776 3843 4004 844 Size: 2932 67 161 3489 Fragments arranged by size: 3489 2932 161 67 BsrBI CCG'CTC Cuts at: 1394 1823 4536 1394 Size: 429 2713 3507 Fragments arranged by size: 3507 2713 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2211 3108 5144 5318 65 Size: 1183 963 897 2036 174 1396 Fragments arranged by size: 2036 1396 1183 963 897 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2135 2255 5293 5480 1700 Size: 110 325 120 3038 187 2869 Fragments arranged by size: 3038 2869 325 187 120 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6649 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6649 BssSI C'ACGA_G Cuts at: 4709 5721 4709 Size: 1012 5637 BstAPI GCAn_nnn'nTGC Cuts at: 794 3033 794 Size: 2239 4410 BstBI TT'CG_AA Cuts at: 5453 5453 Size: 6649 BstEII G'GTnAC_C Cuts at: 1184 3688 3830 1184 Size: 2504 142 4003 Fragments arranged by size: 4003 2504 142 BstXI CCAn_nnnn'nTGG Cuts at: 2059 2576 3520 3937 2059 Size: 517 944 417 4771 Fragments arranged by size: 4771 944 517 417 BstYI r'GATC_y Cuts at: 1972 2484 2892 3103 3979 4346 4731 4748 6200 Size: 512 408 211 876 367 385 17 1452 Cuts at: 6200 6286 6298 6644 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 876 512 408 385 367 346 211 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3888 3936 513 Size: 552 939 1884 48 3226 Fragments arranged by size: 3226 1884 939 552 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5910 Fragments arranged by size: 5910 683 56 BtsI GCAGTG_nn' Cuts at: 2189 2245 3269 3739 3784 3814 4165 4959 4979 Size: 56 1024 470 45 30 351 794 20 Cuts at: 4979 2189 Size: 3859 Fragments arranged by size: 3859 1024 794 470 351 56 45 30 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6649 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6614 DraI TTT'AAA Cuts at: 3953 3953 Size: 6649 DraIII CAC_nnn'GTG Cuts at: 3830 3830 Size: 6649 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5656 809 Size: 507 4340 1802 Fragments arranged by size: 4340 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3054 3129 3522 Size: 54 1425 50 275 18 1224 75 393 Cuts at: 3522 4096 4985 6614 8 Size: 574 889 1629 43 Fragments arranged by size: 1629 1425 1224 889 574 393 275 75 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4096 1537 Size: 275 2284 4090 Fragments arranged by size: 4090 2284 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2813 3089 3135 4579 1353 Size: 1460 276 46 1444 3423 Fragments arranged by size: 3423 1460 1444 276 46 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2516 3192 5236 5610 5756 387 Size: 1064 138 927 676 2044 374 146 1280 Fragments arranged by size: 2044 1280 1064 927 676 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2081 2125 2157 2260 2489 2645 3276 Size: 393 449 44 32 103 229 156 631 Cuts at: 3276 3893 4712 5309 6096 6364 1239 Size: 617 819 597 787 268 1524 Fragments arranged by size: 1524 819 787 631 617 597 449 393 268 229 156 103 44 32 EcoNI CCTnn'n_nnAGG Cuts at: 2087 2087 Size: 6649 EcoO109I rG'GnC_Cy Cuts at: 2048 2080 2225 2271 3043 3227 3296 3325 3756 Size: 32 145 46 772 184 69 29 431 Cuts at: 3756 3902 2048 Size: 146 4795 Fragments arranged by size: 4795 772 431 184 146 145 69 46 32 29 EcoRI G'AATT_C Cuts at: 2953 3958 4336 2953 Size: 1005 378 5266 Fragments arranged by size: 5266 1005 378 FspI TGC'GCA Cuts at: 3119 5155 3119 Size: 2036 4613 HaeII r_GCGC'y Cuts at: 1678 2074 5796 1678 Size: 396 3722 2531 Fragments arranged by size: 3722 2531 396 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2993 3025 3088 3120 5290 Size: 32 135 32 1421 32 63 32 2170 Cuts at: 5290 5322 5364 5396 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2170 1421 135 63 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3993 4239 669 Size: 988 281 2055 246 3079 Fragments arranged by size: 3079 2055 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6649 HpaI GTT'AAC Cuts at: 4239 4239 Size: 6649 KpnI G_GTAC'C Cuts at: 3974 3974 Size: 6649 MfeI C'AATT_G Cuts at: 4248 4248 Size: 6649 MluI A'CGCG_T Cuts at: 6525 6525 Size: 6649 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2187 5763 5947 1305 Size: 882 3576 184 2007 Fragments arranged by size: 3576 2007 882 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3131 10 Size: 54 1425 343 1299 3528 Fragments arranged by size: 3528 1425 1299 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2846 3113 4607 4966 5125 518 Size: 648 1680 267 1494 359 159 2042 Fragments arranged by size: 2042 1680 1494 648 359 267 159 MspA1I CmG'CkG Cuts at: 1606 2145 2467 3254 4065 4742 5890 6135 1606 Size: 539 322 787 811 677 1148 245 2120 Fragments arranged by size: 2120 1148 811 787 677 539 322 245 NaeI GCC'GGC Cuts at: 2137 2257 2137 Size: 120 6529 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5158 Fragments arranged by size: 5158 939 552 NdeI CA'TA_TG Cuts at: 387 2670 387 Size: 2283 4366 NgoMIV G'CCGG_C Cuts at: 2135 2255 2135 Size: 120 6529 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6649 NotI GC'GGCC_GC Cuts at: 4096 4096 Size: 6649 NspI r_CATG'y Cuts at: 1132 1229 2135 4007 5552 1132 Size: 97 906 1872 1545 2229 Fragments arranged by size: 2229 1872 1545 906 97 PasI CC'CwG_GG Cuts at: 1348 2267 2339 2459 3209 3752 1348 Size: 919 72 120 750 543 4245 Fragments arranged by size: 4245 919 750 543 120 72 PciI A'CATG_T Cuts at: 5548 5548 Size: 6649 PflMI CCAn_nnn'nTGG Cuts at: 1108 2590 2717 2770 1108 Size: 1482 127 53 4987 Fragments arranged by size: 4987 1482 127 53 PfoI T'CCnGG_A Cuts at: 3397 3397 Size: 6649 PmeI GTTT'AAAC Cuts at: 3953 3953 Size: 6649 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4718 4750 6231 6263 4718 Size: 32 1481 32 5104 Fragments arranged by size: 5104 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2080 3325 2080 Size: 1245 5404 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6649 PsiI TTA'TAA Cuts at: 4219 4219 Size: 6649 PspOMI G'GGCC_C Cuts at: 2047 2225 2270 3042 3450 3755 2047 Size: 178 45 772 408 305 4941 Fragments arranged by size: 4941 772 408 305 178 45 PstI C_TGCA'G Cuts at: 830 3324 3901 4001 830 Size: 2494 577 100 3478 Fragments arranged by size: 3478 2494 577 100 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6649 PvuII CAG'CTG Cuts at: 2145 3254 2145 Size: 1109 5540 SalI G'TCGA_C Cuts at: 1936 3991 1936 Size: 2055 4594 SapI GCTCTTCn'nnn_ Cuts at: 3135 3135 Size: 6649 SbfI CC_TGCA'GG Cuts at: 3324 3901 4001 3324 Size: 577 100 5972 Fragments arranged by size: 5972 577 100 ScaI AGT'ACT Cuts at: 2705 4897 6609 2705 Size: 2192 1712 2745 Fragments arranged by size: 2745 2192 1712 SfcI C'TryA_G Cuts at: 826 1045 2830 3320 3897 3997 5132 5813 6004 Size: 219 1785 490 577 100 1135 681 191 Cuts at: 6004 826 Size: 1471 Fragments arranged by size: 1785 1471 1135 681 577 490 219 191 100 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6649 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6649 SmaI CCC'GGG Cuts at: 1872 3804 3976 1872 Size: 1932 172 4545 Fragments arranged by size: 4545 1932 172 SmlI C'TyrA_G Cuts at: 820 1017 1943 2351 2855 3440 4753 5654 5916 Size: 197 926 408 504 585 1313 901 262 Cuts at: 5916 820 Size: 1553 Fragments arranged by size: 1553 1313 926 901 585 504 408 262 197 SnaBI TAC'GTA Cuts at: 493 4440 493 Size: 3947 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6649 SphI G_CATG'C Cuts at: 1132 2135 4007 1132 Size: 1003 1872 3774 Fragments arranged by size: 3774 1872 1003 SspI AAT'ATT Cuts at: 5 52 4573 5 Size: 47 4521 2081 Fragments arranged by size: 4521 2081 47 StuI AGG'CCT Cuts at: 1517 2474 2513 2540 3387 1517 Size: 957 39 27 847 4779 Fragments arranged by size: 4779 957 847 39 27 StyI C'CwwG_G Cuts at: 513 1065 2004 2074 3577 4088 513 Size: 552 939 70 1503 511 3074 Fragments arranged by size: 3074 1503 939 552 511 70 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3300 3514 3818 4676 4693 4846 5031 Size: 1255 1720 214 304 858 17 153 185 Cuts at: 5031 5476 325 Size: 445 1498 Fragments arranged by size: 1720 1498 1255 858 445 304 214 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2673 2703 4895 Size: 274 80 33 51 1443 696 30 2192 Cuts at: 4895 6607 96 Size: 1712 138 Fragments arranged by size: 2192 1712 1443 696 274 138 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2085 2117 2957 2989 3332 3364 6609 6641 2085 Size: 32 840 32 343 32 3245 32 2093 Fragments arranged by size: 3245 2093 840 343 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6649 XbaI T'CTAG_A Cuts at: 3985 3985 Size: 6649 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2737 3238 2737 Size: 501 6148 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6649 XmaI C'CCGG_G Cuts at: 1870 3802 3974 1870 Size: 1932 172 4545 Fragments arranged by size: 4545 1932 172 XmnI GAAnn'nnTTC Cuts at: 4778 4778 Size: 6649 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2035 2404 4457 276 Size: 53 83 186 936 501 369 2053 2468 Fragments arranged by size: 2468 2053 936 501 369 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI