(Circular) (Six-base) MAPSORT of: pFN21AB9728.seq Check: 9270 from: 1 to: 6037 pFN21AB9728 6037 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3847 278 Size: 53 83 186 936 2311 2468 Fragments arranged by size: 2468 2311 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3380 1937 Size: 1443 4594 Acc65I G'GTAC_C Cuts at: 2953 3358 2953 Size: 405 5632 AclI AA'CG_TT Cuts at: 1563 4164 4537 1563 Size: 2601 373 3063 Fragments arranged by size: 3063 2601 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2575 4100 5484 5752 1632 Size: 943 1525 1384 268 1917 Fragments arranged by size: 1917 1525 1384 943 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5840 AflIII A'CryG_T Cuts at: 1203 2829 4936 5913 1203 Size: 1626 2107 977 1327 Fragments arranged by size: 2107 1626 1327 977 AgeI A'CCGG_T Cuts at: 4868 4868 Size: 6037 AhdI GACnn_n'nnGTC Cuts at: 4766 4766 Size: 6037 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6037 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3078 1184 Size: 1894 4143 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2552 2584 2552 Size: 32 6005 AlwNI CAG_nnn'CTG Cuts at: 2392 3780 5352 2392 Size: 1388 1572 3077 Fragments arranged by size: 3077 1572 1388 ApaLI G'TGCA_C Cuts at: 4094 5250 4094 Size: 1156 4881 ApoI r'AATT_y Cuts at: 1493 3346 3572 3724 5833 1493 Size: 1853 226 152 2109 1697 Fragments arranged by size: 2109 1853 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4591 160 Size: 872 3559 1606 Fragments arranged by size: 3559 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6037 AvaI C'yCGr_G Cuts at: 1870 1943 2281 2297 2498 2842 2885 3362 1870 Size: 73 338 16 201 344 43 477 4545 Fragments arranged by size: 4545 477 344 338 201 73 43 16 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2945 2978 2945 Size: 33 6004 BamHI G'GATC_C Cuts at: 3367 3734 3367 Size: 367 5670 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2249 2264 2420 2689 2701 Size: 325 217 627 462 15 156 269 12 Cuts at: 2701 2777 2872 2953 3035 3358 4813 618 Size: 76 95 81 82 323 1455 1842 Fragments arranged by size: 1842 1455 627 462 325 323 269 217 156 95 82 81 76 15 12 BanII G_rGCy'C Cuts at: 1391 2093 2282 2351 2567 3011 3029 3356 1391 Size: 702 189 69 216 444 18 327 4072 Fragments arranged by size: 4072 702 444 327 216 189 69 18 BbeI G_GCGC'C Cuts at: 2253 2268 2424 2693 2705 2781 3039 2253 Size: 15 156 269 12 76 258 5251 Fragments arranged by size: 5251 269 258 156 76 15 12 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5066 BbvCI CC'TCA_GC Cuts at: 3268 3268 Size: 6037 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4228 4262 1903 Size: 34 2291 34 3678 Fragments arranged by size: 3678 2291 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3929 5139 3929 Size: 1210 4827 BclI T'GATC_A Cuts at: 1548 1548 Size: 6037 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2213 2273 2727 Size: 107 122 71 631 605 541 60 454 Cuts at: 2727 3095 4648 136 Size: 368 1553 1525 Fragments arranged by size: 1553 1525 631 605 541 454 368 122 107 71 60 BglII A'GATC_T Cuts at: 2379 6032 2379 Size: 3653 2384 BlpI GC'TnA_GC Cuts at: 2372 3454 2372 Size: 1082 4955 Bme1580I G_kGCm'C Cuts at: 1790 2877 4098 5254 1790 Size: 1087 1221 1156 2573 Fragments arranged by size: 2573 1221 1156 1087 BmgBI CAC'GTC Cuts at: 1318 2821 1318 Size: 1503 4534 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3768 4726 442 Size: 416 53 83 399 24 2351 958 1753 Fragments arranged by size: 2351 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3276 1056 Size: 2220 3817 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2155 2581 3187 2155 Size: 426 606 5005 Fragments arranged by size: 5005 606 426 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2057 3043 3094 3152 3192 4697 1239 Size: 818 986 51 58 40 1505 2579 Fragments arranged by size: 2579 1505 986 818 58 51 40 Bpu10I CC'TnA_GC Cuts at: 3268 3268 Size: 6037 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2597 3035 4162 5027 5325 2597 Size: 438 1127 865 298 3309 Fragments arranged by size: 3309 1127 865 438 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2126 4700 882 Size: 617 627 2574 2219 Fragments arranged by size: 2574 2219 627 617 BsaAI yAC'GTr Cuts at: 493 1204 3828 493 Size: 711 2624 2702 Fragments arranged by size: 2702 2624 711 BsaBI GATnn'nnATC Cuts at: 6031 6031 Size: 6037 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2250 2265 2421 2657 Size: 53 83 186 936 717 15 156 236 Cuts at: 2657 2690 2702 2766 2778 2892 3036 3071 3844 Size: 33 12 64 12 114 144 35 773 Cuts at: 3844 4226 4861 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 773 717 635 382 236 186 156 144 114 83 64 53 35 33 15 12 12 BsaWI w'CCGG_w Cuts at: 4470 4868 5142 5289 5700 4470 Size: 398 274 147 411 4807 Fragments arranged by size: 4807 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2552 2582 1178 Size: 30 165 30 1149 30 4633 Fragments arranged by size: 4633 1149 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2131 2134 2137 2179 2573 1186 Size: 945 3 3 42 394 4650 Fragments arranged by size: 4650 945 394 42 3 3 BseYI C'CCAG_C Cuts at: 2354 2783 3074 5240 5736 2354 Size: 429 291 2166 496 2655 Fragments arranged by size: 2655 2166 496 429 291 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2071 3089 1163 Size: 573 335 1018 4111 Fragments arranged by size: 4111 1018 573 335 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2582 2904 3487 4248 5276 1540 Size: 275 186 581 322 583 761 1028 2301 Fragments arranged by size: 2301 1028 761 583 581 322 275 186 BsiHKAI G_wGCw'C Cuts at: 2282 2351 3029 4098 4183 5254 2282 Size: 69 678 1069 85 1071 3065 Fragments arranged by size: 3065 1071 1069 678 85 69 BsmI GAATG_Cn' Cuts at: 2993 3548 3641 2993 Size: 555 93 5389 Fragments arranged by size: 5389 555 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2093 2282 2351 2567 2877 3011 3029 Size: 399 303 189 69 216 310 134 18 Cuts at: 3029 3356 4098 4183 5254 1391 Size: 327 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 327 310 303 216 189 134 85 69 18 BspEI T'CCGG_A Cuts at: 5700 5700 Size: 6037 BspHI T'CATG_A Cuts at: 3926 5656 3926 Size: 1730 4307 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3392 844 Size: 2548 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2038 2259 2640 3924 1394 Size: 429 215 221 381 1284 3507 Fragments arranged by size: 3507 1284 429 381 221 215 BsrDI GCAATG_nn' Cuts at: 65 1248 4532 4706 65 Size: 1183 3284 174 1396 Fragments arranged by size: 3284 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2029 2577 2768 4681 4868 1700 Size: 110 219 548 191 1913 187 2869 Fragments arranged by size: 2869 1913 548 219 191 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6037 BssHII G'CGCG_C Cuts at: 1925 2067 2399 1925 Size: 142 332 5563 Fragments arranged by size: 5563 332 142 BssSI C'ACGA_G Cuts at: 3004 4097 5109 3004 Size: 1093 1012 3932 Fragments arranged by size: 3932 1093 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6037 BstBI TT'CG_AA Cuts at: 4841 4841 Size: 6037 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6037 BstXI CCAn_nnnn'nTGG Cuts at: 2857 2857 Size: 6037 BstYI r'GATC_y Cuts at: 1972 2379 3367 3734 4119 4136 5588 5674 5686 Size: 407 988 367 385 17 1452 86 12 Cuts at: 5686 6032 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 988 407 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2490 2490 Size: 6037 BtgI C'CryG_G Cuts at: 513 1065 2416 2754 2877 2970 513 Size: 552 1351 338 123 93 3580 Fragments arranged by size: 3580 1351 552 338 123 93 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2305 487 Size: 683 56 1079 4219 Fragments arranged by size: 4219 1079 683 56 BtsI GCAGTG_nn' Cuts at: 3553 4347 4367 3553 Size: 794 20 5223 Fragments arranged by size: 5223 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6037 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6002 DraI TTT'AAA Cuts at: 3341 3341 Size: 6037 DraIII CAC_nnn'GTG Cuts at: 2632 2632 Size: 6037 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5044 809 Size: 507 3728 1802 Fragments arranged by size: 3728 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2199 2337 2538 Size: 54 1425 50 275 18 369 138 201 Cuts at: 2538 2579 2859 2901 2967 3484 4373 6002 8 Size: 41 280 42 66 517 889 1629 43 Fragments arranged by size: 1629 1425 889 517 369 280 275 201 138 66 54 50 43 42 41 18 EagI C'GGCC_G Cuts at: 1537 1812 2579 2901 3484 1537 Size: 275 767 322 583 4090 Fragments arranged by size: 4090 767 583 322 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3967 1353 Size: 2614 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2030 2152 2561 2611 4624 4998 Size: 1064 138 441 122 409 50 2013 374 Cuts at: 4998 5144 387 Size: 146 1280 Fragments arranged by size: 2013 1280 1064 441 409 374 146 138 122 50 EcoICRI GAG'CTC Cuts at: 2280 2349 3027 2280 Size: 69 678 5290 Fragments arranged by size: 5290 678 69 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 2575 3043 3094 3152 3192 4100 Size: 393 425 518 468 51 58 40 908 Cuts at: 4100 4697 5484 5752 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 908 787 597 518 468 425 393 268 58 51 40 EcoO109I rG'GnC_Cy Cuts at: 2494 2882 3166 2494 Size: 388 284 5365 Fragments arranged by size: 5365 388 284 EcoRI G'AATT_C Cuts at: 3346 3724 3346 Size: 378 5659 FspI TGC'GCA Cuts at: 4543 4543 Size: 6037 HaeII r_GCGC'y Cuts at: 1678 2208 2253 2268 2365 2424 2612 2693 2705 Size: 530 45 15 97 59 188 81 12 Cuts at: 2705 2781 2982 3039 5184 1678 Size: 76 201 57 2145 2531 Fragments arranged by size: 2531 2145 530 201 188 97 81 76 59 57 45 15 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3295 3327 4678 4710 4752 Size: 32 135 32 1723 32 1351 32 42 Cuts at: 4752 4784 1373 Size: 32 2626 Fragments arranged by size: 2626 1723 1351 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3381 3627 669 Size: 988 281 1443 246 3079 Fragments arranged by size: 3079 1443 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6037 HpaI GTT'AAC Cuts at: 3627 3627 Size: 6037 KasI G'GCGC_C Cuts at: 2249 2264 2420 2689 2701 2777 3035 2249 Size: 15 156 269 12 76 258 5251 Fragments arranged by size: 5251 269 258 156 76 15 12 KpnI G_GTAC'C Cuts at: 2957 3362 2957 Size: 405 5632 MfeI C'AATT_G Cuts at: 3636 3636 Size: 6037 MluI A'CGCG_T Cuts at: 5913 5913 Size: 6037 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5151 5335 1305 Size: 3846 184 2007 Fragments arranged by size: 3846 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2339 2861 10 Size: 54 1425 343 507 522 3186 Fragments arranged by size: 3186 1425 522 507 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2333 2855 3995 4354 4513 518 Size: 648 1167 522 1140 359 159 2042 Fragments arranged by size: 2042 1167 1140 648 522 359 159 MspA1I CmG'CkG Cuts at: 1606 2418 2756 2865 2879 3453 4130 5278 5523 Size: 812 338 109 14 574 677 1148 245 Cuts at: 5523 1606 Size: 2120 Fragments arranged by size: 2120 1148 812 677 574 338 245 109 14 NaeI GCC'GGC Cuts at: 2031 2579 2770 2031 Size: 548 191 5298 Fragments arranged by size: 5298 548 191 NarI GG'CG_CC Cuts at: 2250 2265 2421 2690 2702 2778 3036 2250 Size: 15 156 269 12 76 258 5251 Fragments arranged by size: 5251 269 258 156 76 15 12 NcoI C'CATG_G Cuts at: 513 1065 2970 513 Size: 552 1905 3580 Fragments arranged by size: 3580 1905 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6037 NgoMIV G'CCGG_C Cuts at: 2029 2577 2768 2029 Size: 548 191 5298 Fragments arranged by size: 5298 548 191 NheI G'CTAG_C Cuts at: 1052 3272 1052 Size: 2220 3817 NotI GC'GGCC_GC Cuts at: 3484 3484 Size: 6037 NruI TCG'CGA Cuts at: 3162 3162 Size: 6037 NspI r_CATG'y Cuts at: 1132 1229 2833 3118 3395 4940 1132 Size: 97 1604 285 277 1545 2229 Fragments arranged by size: 2229 1604 1545 285 277 97 PasI CC'CwG_GG Cuts at: 1348 2671 1348 Size: 1323 4714 PciI A'CATG_T Cuts at: 2829 4936 2829 Size: 2107 3930 PflMI CCAn_nnn'nTGG Cuts at: 1108 2632 1108 Size: 1524 4513 PmeI GTTT'AAAC Cuts at: 3341 3341 Size: 6037 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2537 2552 2569 2584 4106 4138 5619 5651 2537 Size: 15 17 15 1522 32 1481 32 2923 Fragments arranged by size: 2923 1522 1481 32 32 17 15 15 PpuMI rG'GwC_Cy Cuts at: 2882 3166 2882 Size: 284 5753 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6037 PsiI TTA'TAA Cuts at: 3607 3607 Size: 6037 PstI C_TGCA'G Cuts at: 830 2397 3389 830 Size: 1567 992 3478 Fragments arranged by size: 3478 1567 992 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6037 PvuII CAG'CTG Cuts at: 2865 2865 Size: 6037 SacI G_AGCT'C Cuts at: 2282 2351 3029 2282 Size: 69 678 5290 Fragments arranged by size: 5290 678 69 SacII CC_GC'GG Cuts at: 2419 2757 2880 2419 Size: 338 123 5576 Fragments arranged by size: 5576 338 123 SalI G'TCGA_C Cuts at: 1936 3379 1936 Size: 1443 4594 SanDI GG'GwC_CC Cuts at: 2882 2882 Size: 6037 SbfI CC_TGCA'GG Cuts at: 2397 3389 2397 Size: 992 5045 ScaI AGT'ACT Cuts at: 4285 5997 4285 Size: 1712 4325 SfcI C'TryA_G Cuts at: 826 1045 2393 3385 4520 5201 5392 826 Size: 219 1348 992 1135 681 191 1471 Fragments arranged by size: 1471 1348 1135 992 681 219 191 SfoI GGC'GCC Cuts at: 2251 2266 2422 2691 2703 2779 3037 2251 Size: 15 156 269 12 76 258 5251 Fragments arranged by size: 5251 269 258 156 76 15 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6037 SgrAI Cr'CCGG_yG Cuts at: 1700 2768 1700 Size: 1068 4969 SmaI CCC'GGG Cuts at: 1872 2844 2887 3364 1872 Size: 972 43 477 4545 Fragments arranged by size: 4545 972 477 43 SmlI C'TyrA_G Cuts at: 820 1017 1943 2612 3050 4141 5042 5304 820 Size: 197 926 669 438 1091 901 262 1553 Fragments arranged by size: 1553 1091 926 901 669 438 262 197 SnaBI TAC'GTA Cuts at: 493 3828 493 Size: 3335 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6037 SphI G_CATG'C Cuts at: 1132 3395 1132 Size: 2263 3774 SspI AAT'ATT Cuts at: 5 52 3961 5 Size: 47 3909 2081 Fragments arranged by size: 3909 2081 47 StuI AGG'CCT Cuts at: 1517 3102 1517 Size: 1585 4452 StyI C'CwwG_G Cuts at: 513 1065 2970 3476 513 Size: 552 1905 506 3074 Fragments arranged by size: 3074 1905 552 506 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4064 4081 4234 4419 4864 325 Size: 1255 2484 17 153 185 445 1498 Fragments arranged by size: 2484 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4283 5995 96 Size: 274 80 33 51 1443 2306 1712 138 Fragments arranged by size: 2306 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5997 6029 5997 Size: 32 6005 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6037 XbaI T'CTAG_A Cuts at: 3373 3373 Size: 6037 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6037 XmaI C'CCGG_G Cuts at: 1870 2842 2885 3362 1870 Size: 972 43 477 4545 Fragments arranged by size: 4545 972 477 43 XmnI GAAnn'nnTTC Cuts at: 4166 4166 Size: 6037 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3845 276 Size: 53 83 186 936 2311 2468 Fragments arranged by size: 2468 2311 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BfrBI BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FseI FspAI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SapI SexAI SfiI SrfI SwaI XcmI