Restriction map for
pFN21AB9596
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BbsI BbvCI
BcgI BciVI BfrI BglII BlnI
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BsiWI BsmI BspDI BspEI
BspMI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DraIII DrdI Eam1105I EarI
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
KpnI Ksp632I MamI MfeI MluI
MroI MseI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SalI SapI SbfI
ScaI SfiI SfuI SnaBI SpeI
Sse8387I SspI StuI SwaI TfiI
Tru9I Tsp45I Tth111I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AlwI 1445 GATCC GGATC(4/5)
AocI 199 CCTAAGG CC^T[ACGT]AGG
Aor51HI 1108 AGCGCT AGC^GCT
ApaI 539 GGGCCC GGGCC^C
AspHI 772 GAGCAC G[AT]GC[AT]^C
BclI 635 TGATCA T^GATCA
BfaI 598 CTAG C^TAG
BpmI 961 CTGGAG CTGGAG(16/14)
BseRI 532 GAGGAG GAGGAG(10/8)
BsiEI 1030 CGGCCG CG[GA][CT]^CG
BsiHKAI 772 GAGCAC G[AT]GC[AT]^C
BsmAI 685 GAGAC GTCTC(1/5)
BsmBI 685 GAGACG CGTCTC(1/5)
Bsp1407I 1013 TGTACA T^GTACA
BspHI 824 TCATGA T^CATGA
BsrDI 1418 GCAATG GCAATG(2/0)
BsrGI 1013 TGTACA T^GTACA
BstYI 1444 AGATCC [GA]^GATC[CT]
Bsu36I 199 CCTAAGG CC^T[ACGT]AGG
EagI 1030 CGGCCG C^GGCCG
EclXI 1030 CGGCCG C^GGCCG
Eco47III 1108 AGCGCT AGC^GCT
Eco52I 1030 CGGCCG C^GGCCG
Eco81I 199 CCTAAGG CC^T[ACGT]AGG
EcoNI 1059 CCTGGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 635 TGATCA T^GATCA
MaeI 598 CTAG C^TAG
MaeIII 431 GTTAC ^GT[ACGT]AC
MflI 1444 AGATCC [GA]^GATC[CT]
PflMI 1368 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 709 CTGCAG CTGCA^G
RcaI 824 TCATGA T^CATGA
SexAI 206 ACCTGGT A^CC[AT]GGT
SgrAI 490 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 547 CTCAAG C^T[CT][GA]AG
SphI 995 GCATGC GCATG^C
SspBI 1013 TGTACA T^GTACA
Tsp509I 67 AATT ^AATT
TspRI 988 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 1368 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1444 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 141 GTAC GT^AC
1014 GTAC
AlwNI 429 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
955 CAGGGCCTG
BalI 101 TGGCCA TGG^CCA
248 TGGCCA
BlpI 690 GCTGAGC GC^T[ACGT]AGC
858 GCTGAGC
Bpu1102I 690 GCTGAGC GC^T[ACGT]AGC
858 GCTGAGC
BsgI 1491 CTGCAC GTGCAG(16/14)
990 GTGCAG
BspLU11I 461 ACATGT A^CATGT
1040 ACATGT
CelII 690 GCTGAGC GC^T[ACGT]AGC
858 GCTGAGC
HinfI 451 GACTC G^A[ACGT]TC
1402 GAGTC
MluNI 101 TGGCCA TGG^CCA
248 TGGCCA
MscI 101 TGGCCA TGG^CCA
248 TGGCCA
MslI 240 CATCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
675 CACCAAGGTG
NcoI 110 CCATGG C^CATGG
245 CCATGG
PleI 451 GACTC GAGTC(4/5)
1402 GAGTC
PpuMI 203 AGGACCT [GA]G^G[AT]CC[CT]
1289 GGGACCT
PvuII 1425 CAGCTG CAG^CTG
1432 CAGCTG
RsaI 141 GTAC GT^AC
1014 GTAC
SfaNI 290 GCATC GCATC(5/9)
356 GCATC
SmaI 864 CCCGGG CCC^GGG
1240 CCCGGG
TaqI 467 TCGA T^CGA
1390 TCGA
TthHB8I 467 TCGA T^CGA
1390 TCGA
XmaCI 864 CCCGGG C^CCGGG
1240 CCCGGG
XmaI 864 CCCGGG C^CCGGG
1240 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AcyI AflIII AluI
AvaI AvaII BanI BanII BbeI
BbvI BcnI BglI BmyI BsaHI
BsaJI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BsrI BssHII
BssKI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII HphI ItaI
KasI KspI MaeII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV NspI SacII
Sau3AI Sau96I ScrFI SfcI SfoI
StyI TseI