(Circular) (Six-base) MAPSORT of: pFN21AB9596.seq Check: 3484 from: 1 to: 6208 pFN21AB9596 6208 bp With 182 enzymes: SgfI * March 28, 2012 12:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4018 278 Size: 53 83 186 936 2482 2468 Fragments arranged by size: 2482 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3551 1937 Size: 1614 4594 Acc65I G'GTAC_C Cuts at: 3529 3529 Size: 6208 AclI AA'CG_TT Cuts at: 1563 4335 4708 1563 Size: 2772 373 3063 Fragments arranged by size: 3063 2772 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2797 4271 5655 5923 1632 Size: 1165 1474 1384 268 1917 Fragments arranged by size: 1917 1474 1384 1165 268 AfeI AGC'GCT Cuts at: 3115 3115 Size: 6208 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6011 AflIII A'CryG_T Cuts at: 1203 2466 2520 3045 3318 5107 6084 1203 Size: 1263 54 525 273 1789 977 1327 Fragments arranged by size: 1789 1327 1263 977 525 273 54 AgeI A'CCGG_T Cuts at: 5039 5039 Size: 6208 AhdI GACnn_n'nnGTC Cuts at: 4937 4937 Size: 6208 AleI CACnn'nnGTG Cuts at: 1166 2684 1166 Size: 1518 4690 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2504 1184 Size: 1320 4888 AlwNI CAG_nnn'CTG Cuts at: 2439 2965 3951 5523 2439 Size: 526 986 1572 3124 Fragments arranged by size: 3124 1572 986 526 ApaI G_GGCC'C Cuts at: 2548 2548 Size: 6208 ApaLI G'TGCA_C Cuts at: 4265 5421 4265 Size: 1156 5052 ApoI r'AATT_y Cuts at: 1493 3517 3743 3895 6004 1493 Size: 2024 226 152 2109 1697 Fragments arranged by size: 2109 2024 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4762 160 Size: 872 3730 1606 Fragments arranged by size: 3730 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 AvaI C'yCGr_G Cuts at: 1870 1943 2270 2702 2869 3088 3245 3401 3533 Size: 73 327 432 167 219 157 156 132 Cuts at: 3533 1870 Size: 4545 Fragments arranged by size: 4545 432 327 219 167 157 156 132 73 BamHI G'GATC_C Cuts at: 3538 3905 3538 Size: 367 5841 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2050 2200 2401 2493 2582 Size: 325 217 627 263 150 201 92 89 Cuts at: 2582 2791 2896 3199 3529 4984 618 Size: 209 105 303 330 1455 1842 Fragments arranged by size: 1842 1455 627 330 325 303 263 217 209 201 150 105 92 89 BanII G_rGCy'C Cuts at: 1391 2548 2702 2870 3527 1391 Size: 1157 154 168 657 4072 Fragments arranged by size: 4072 1157 657 168 154 BbeI G_GCGC'C Cuts at: 2204 2497 2586 2795 3203 2204 Size: 293 89 209 408 5209 Fragments arranged by size: 5209 408 293 209 89 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5237 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4399 4433 1903 Size: 34 50 34 2378 34 3678 Fragments arranged by size: 3678 2378 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4100 5310 4100 Size: 1210 4998 BclI T'GATC_A Cuts at: 1548 2640 1548 Size: 1092 5116 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2118 2800 Size: 107 122 71 631 605 337 109 682 Cuts at: 2800 3141 3257 4819 136 Size: 341 116 1562 1525 Fragments arranged by size: 1562 1525 682 631 605 341 337 122 116 109 107 71 BglII A'GATC_T Cuts at: 6203 6203 Size: 6208 BlpI GC'TnA_GC Cuts at: 2696 2864 3625 2696 Size: 168 761 5279 Fragments arranged by size: 5279 761 168 Bme1580I G_kGCm'C Cuts at: 1790 2055 2406 2548 4269 5425 1790 Size: 265 351 142 1721 1156 2573 Fragments arranged by size: 2573 1721 1156 351 265 142 BmgBI CAC'GTC Cuts at: 1318 2521 3102 1318 Size: 1203 581 4424 Fragments arranged by size: 4424 1203 581 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3939 4897 442 Size: 416 53 83 399 24 2522 958 1753 Fragments arranged by size: 2522 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6208 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3094 3094 Size: 6208 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2987 4868 1239 Size: 1748 1881 2579 Fragments arranged by size: 2579 1881 1748 Bpu10I CC'TnA_GC Cuts at: 2060 2958 3110 2060 Size: 898 152 5158 Fragments arranged by size: 5158 898 152 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2537 4333 5198 5496 2537 Size: 1796 865 298 3249 Fragments arranged by size: 3249 1796 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4871 882 Size: 617 3372 2219 Fragments arranged by size: 3372 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3999 493 Size: 711 2795 2702 Fragments arranged by size: 2795 2702 711 BsaBI GATnn'nnATC Cuts at: 6202 6202 Size: 6208 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2121 2201 2494 2573 Size: 53 83 186 936 588 80 293 79 Cuts at: 2573 2583 2610 2657 2792 3200 3236 4015 4397 Size: 10 27 47 135 408 36 779 382 Cuts at: 4397 5032 275 Size: 635 1451 Fragments arranged by size: 1451 936 779 635 588 408 382 293 186 135 83 80 79 53 47 36 27 10 BsaWI w'CCGG_w Cuts at: 4641 5039 5313 5460 5871 4641 Size: 398 274 147 411 4978 Fragments arranged by size: 4978 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2441 2471 1178 Size: 30 165 30 1038 30 4915 Fragments arranged by size: 4915 1038 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2552 1186 Size: 1366 4842 BseYI C'CCAG_C Cuts at: 3222 3228 3330 3379 5411 5907 3222 Size: 6 102 49 2032 496 3523 Fragments arranged by size: 3523 2032 496 102 49 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3016 3481 1163 Size: 573 1280 465 3890 Fragments arranged by size: 3890 1280 573 465 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3038 3658 4419 5447 1540 Size: 275 186 1037 620 761 1028 2301 Fragments arranged by size: 2301 1037 1028 761 620 275 186 BsiHKAI G_wGCw'C Cuts at: 2781 4269 4354 5425 2781 Size: 1488 85 1071 3564 Fragments arranged by size: 3564 1488 1071 85 BsmI GAATG_Cn' Cuts at: 3719 3812 3719 Size: 93 6115 BsmBI CGTCTCn'nnnn_ Cuts at: 2684 2684 Size: 6208 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2406 2548 2702 2781 2870 3527 Size: 399 265 351 142 154 79 89 657 Cuts at: 3527 4269 4354 5425 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 657 399 351 265 154 142 89 85 79 BspEI T'CCGG_A Cuts at: 5871 5871 Size: 6208 BspHI T'CATG_A Cuts at: 2829 4097 5827 2829 Size: 1268 1730 3210 Fragments arranged by size: 3210 1730 1268 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3563 844 Size: 2719 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2166 2542 3034 3093 4095 1394 Size: 429 343 376 492 59 1002 3507 Fragments arranged by size: 3507 1002 492 429 376 343 59 BsrDI GCAATG_nn' Cuts at: 65 1248 3430 4703 4877 65 Size: 1183 2182 1273 174 1396 Fragments arranged by size: 2182 1396 1273 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2496 2671 2750 3037 3131 3214 3375 Size: 110 686 175 79 287 94 83 161 Cuts at: 3375 4852 5039 1700 Size: 1477 187 2869 Fragments arranged by size: 2869 1477 686 287 187 175 161 110 94 83 79 BsrGI T'GTAC_A Cuts at: 96 3018 96 Size: 2922 3286 BssHII G'CGCG_C Cuts at: 1925 2012 2709 3144 1925 Size: 87 697 435 4989 Fragments arranged by size: 4989 697 435 87 BssSI C'ACGA_G Cuts at: 4268 5280 4268 Size: 1012 5196 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6208 BstBI TT'CG_AA Cuts at: 5012 5012 Size: 6208 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6208 BstXI CCAn_nnnn'nTGG Cuts at: 2041 2116 3419 2041 Size: 75 1303 4830 Fragments arranged by size: 4830 1303 75 BstYI r'GATC_y Cuts at: 1972 3449 3538 3905 4290 4307 5759 5845 5857 Size: 1477 89 367 385 17 1452 86 12 Cuts at: 5857 6203 1972 Size: 346 1977 Fragments arranged by size: 1977 1477 1452 385 367 346 89 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2205 2205 Size: 6208 BtgI C'CryG_G Cuts at: 513 1065 2115 2196 2250 2787 3156 3178 3255 Size: 552 1050 81 54 537 369 22 77 Cuts at: 3255 513 Size: 3466 Fragments arranged by size: 3466 1050 552 537 369 81 77 54 22 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2221 3023 487 Size: 683 56 995 802 3672 Fragments arranged by size: 3672 995 802 683 56 BtsI GCAGTG_nn' Cuts at: 3724 4518 4538 3724 Size: 794 20 5394 Fragments arranged by size: 5394 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6208 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6173 DraI TTT'AAA Cuts at: 3512 3512 Size: 6208 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5215 809 Size: 507 3899 1802 Fragments arranged by size: 3899 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2106 2131 2253 Size: 54 1425 50 275 18 276 25 122 Cuts at: 2253 2589 2944 3035 3133 3181 3313 3655 4544 Size: 336 355 91 98 48 132 342 889 Cuts at: 4544 6173 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 355 342 336 276 275 132 122 98 91 54 50 48 43 25 18 EagI C'GGCC_G Cuts at: 1537 1812 3035 3655 1537 Size: 275 1223 620 4090 Fragments arranged by size: 4090 1223 620 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4138 1353 Size: 2785 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2354 2871 4795 5169 5315 387 Size: 1064 138 765 517 1924 374 146 1280 Fragments arranged by size: 1924 1280 1064 765 517 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2797 2987 4271 4868 5655 5923 1239 Size: 393 1165 190 1284 597 787 268 1524 Fragments arranged by size: 1524 1284 1165 787 597 393 268 190 EcoNI CCTnn'n_nnAGG Cuts at: 3068 3068 Size: 6208 EcoO109I rG'GnC_Cy Cuts at: 2209 2962 3073 3295 2209 Size: 753 111 222 5122 Fragments arranged by size: 5122 753 222 111 EcoRI G'AATT_C Cuts at: 3517 3895 3517 Size: 378 5830 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2302 2302 Size: 6208 FspI TGC'GCA Cuts at: 4714 4714 Size: 6208 HaeII r_GCGC'y Cuts at: 1678 2204 2497 2506 2586 2669 2795 2912 3117 Size: 526 293 9 80 83 126 117 205 Cuts at: 3117 3203 5355 1678 Size: 86 2152 2531 Fragments arranged by size: 2531 2152 526 293 205 126 117 86 83 80 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2441 2473 2642 2674 4849 Size: 32 135 32 869 32 169 32 2175 Cuts at: 4849 4881 4923 4955 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2175 869 169 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3552 3798 669 Size: 988 281 1614 246 3079 Fragments arranged by size: 3079 1614 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6208 HpaI GTT'AAC Cuts at: 3798 3798 Size: 6208 KasI G'GCGC_C Cuts at: 2200 2493 2582 2791 3199 2200 Size: 293 89 209 408 5209 Fragments arranged by size: 5209 408 293 209 89 KpnI G_GTAC'C Cuts at: 3533 3533 Size: 6208 MfeI C'AATT_G Cuts at: 3807 3807 Size: 6208 MluI A'CGCG_T Cuts at: 6084 6084 Size: 6208 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2052 5322 5506 1305 Size: 747 3270 184 2007 Fragments arranged by size: 3270 2007 747 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2108 2255 10 Size: 54 1425 343 276 147 3963 Fragments arranged by size: 3963 1425 343 276 147 54 MslI CAynn'nnrTG Cuts at: 518 1166 2249 2684 4166 4525 4684 518 Size: 648 1083 435 1482 359 159 2042 Fragments arranged by size: 2042 1482 1083 648 435 359 159 MspA1I CmG'CkG Cuts at: 1606 2198 2789 2905 3257 3291 3432 3439 3624 Size: 592 591 116 352 34 141 7 185 Cuts at: 3624 4301 5449 5694 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 592 591 352 245 185 141 116 34 7 NaeI GCC'GGC Cuts at: 2498 2752 3039 3133 3216 2498 Size: 254 287 94 83 5490 Fragments arranged by size: 5490 287 254 94 83 NarI GG'CG_CC Cuts at: 2201 2494 2583 2792 3200 2201 Size: 293 89 209 408 5209 Fragments arranged by size: 5209 408 293 209 89 NcoI C'CATG_G Cuts at: 513 1065 2115 2250 513 Size: 552 1050 135 4471 Fragments arranged by size: 4471 1050 552 135 NdeI CA'TA_TG Cuts at: 387 387 Size: 6208 NgoMIV G'CCGG_C Cuts at: 2496 2750 3037 3131 3214 2496 Size: 254 287 94 83 5490 Fragments arranged by size: 5490 287 254 94 83 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6208 NotI GC'GGCC_GC Cuts at: 3655 3655 Size: 6208 NspI r_CATG'y Cuts at: 1132 1229 2470 3004 3049 3566 5111 1132 Size: 97 1241 534 45 517 1545 2229 Fragments arranged by size: 2229 1545 1241 534 517 97 45 PasI CC'CwG_GG Cuts at: 1348 2041 1348 Size: 693 5515 PciI A'CATG_T Cuts at: 2466 3045 5107 2466 Size: 579 2062 3567 Fragments arranged by size: 3567 2062 579 PflMI CCAn_nnn'nTGG Cuts at: 1108 3379 1108 Size: 2271 3937 PmeI GTTT'AAAC Cuts at: 3512 3512 Size: 6208 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4277 4309 5790 5822 4277 Size: 32 1481 32 4663 Fragments arranged by size: 4663 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2209 3295 2209 Size: 1086 5122 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6208 PsiI TTA'TAA Cuts at: 3778 3778 Size: 6208 PspOMI G'GGCC_C Cuts at: 2544 2544 Size: 6208 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2282 2314 2282 Size: 32 6176 PstI C_TGCA'G Cuts at: 830 2718 3560 830 Size: 1888 842 3478 Fragments arranged by size: 3478 1888 842 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6208 PvuII CAG'CTG Cuts at: 3432 3439 3432 Size: 7 6201 SacII CC_GC'GG Cuts at: 2199 2790 3258 2199 Size: 591 468 5149 Fragments arranged by size: 5149 591 468 SalI G'TCGA_C Cuts at: 1936 3550 1936 Size: 1614 4594 SbfI CC_TGCA'GG Cuts at: 3560 3560 Size: 6208 ScaI AGT'ACT Cuts at: 4456 6168 4456 Size: 1712 4496 SexAI A'CCwGG_T Cuts at: 2211 2211 Size: 6208 SfcI C'TryA_G Cuts at: 826 1045 2227 2714 3445 3556 4691 5372 5563 Size: 219 1182 487 731 111 1135 681 191 Cuts at: 5563 826 Size: 1471 Fragments arranged by size: 1471 1182 1135 731 681 487 219 191 111 SfoI GGC'GCC Cuts at: 2202 2495 2584 2793 3201 2202 Size: 293 89 209 408 5209 Fragments arranged by size: 5209 408 293 209 89 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 SgrAI Cr'CCGG_yG Cuts at: 1700 2496 1700 Size: 796 5412 SmaI CCC'GGG Cuts at: 1872 2871 3247 3535 1872 Size: 999 376 288 4545 Fragments arranged by size: 4545 999 376 288 SmlI C'TyrA_G Cuts at: 820 1017 1943 2552 4312 5213 5475 820 Size: 197 926 609 1760 901 262 1553 Fragments arranged by size: 1760 1553 926 901 609 262 197 SnaBI TAC'GTA Cuts at: 493 3999 493 Size: 3506 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6208 SphI G_CATG'C Cuts at: 1132 3004 3566 1132 Size: 1872 562 3774 Fragments arranged by size: 3774 1872 562 SspI AAT'ATT Cuts at: 5 52 4132 5 Size: 47 4080 2081 Fragments arranged by size: 4080 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6208 StyI C'CwwG_G Cuts at: 513 1065 2115 2250 2526 2577 2616 2682 3647 Size: 552 1050 135 276 51 39 66 965 Cuts at: 3647 513 Size: 3074 Fragments arranged by size: 3074 1050 965 552 276 135 66 51 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4235 4252 4405 4590 5035 325 Size: 1255 2655 17 153 185 445 1498 Fragments arranged by size: 2655 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2145 3018 4454 Size: 274 80 33 51 1443 168 873 1436 Cuts at: 4454 6166 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1436 873 274 168 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6168 6200 6168 Size: 32 6176 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6208 XbaI T'CTAG_A Cuts at: 3544 3544 Size: 6208 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6208 XmaI C'CCGG_G Cuts at: 1870 2869 3245 3533 1870 Size: 999 376 288 4545 Fragments arranged by size: 4545 999 376 288 XmnI GAAnn'nnTTC Cuts at: 4337 4337 Size: 6208 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4016 276 Size: 53 83 186 936 2482 2468 Fragments arranged by size: 2482 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspXI RsrII SacI SanDI SapI SfiI SrfI SwaI XcmI