(Linear) (Six-base) MAPSORT of: KIBB9596.seq Check: 5883 from: 1 to: 1503 KIBB9596.orf With 182 enzymes: SgfI * March 22, 2012 15:46 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 792 1503 Size: 792 711 AfeI AGC'GCT Cuts at: 0 1110 1503 Size: 1110 393 AflIII A'CryG_T Cuts at: 0 461 515 1040 1313 1503 Size: 461 54 525 273 190 Fragments arranged by size: 525 461 273 190 54 AleI CACnn'nnGTG Cuts at: 0 679 1503 Size: 679 824 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 499 1503 Size: 499 1004 AlwNI CAG_nnn'CTG Cuts at: 0 434 960 1503 Size: 434 526 543 Fragments arranged by size: 543 526 434 ApaI G_GGCC'C Cuts at: 0 543 1503 Size: 543 960 AvaI C'yCGr_G Cuts at: 0 265 697 864 1083 1240 1396 1503 Size: 265 432 167 219 157 156 107 Fragments arranged by size: 432 265 219 167 157 156 107 BanI G'GyrC_C Cuts at: 0 45 195 396 488 577 786 891 1194 Size: 45 150 201 92 89 209 105 303 Cuts at: 1194 1503 Size: 309 Fragments arranged by size: 309 303 209 201 150 105 92 89 45 BanII G_rGCy'C Cuts at: 0 543 697 865 1503 Size: 543 154 168 638 Fragments arranged by size: 638 543 168 154 BbeI G_GCGC'C Cuts at: 0 199 492 581 790 1198 1503 Size: 199 293 89 209 408 305 Fragments arranged by size: 408 305 293 209 199 89 BclI T'GATC_A Cuts at: 0 635 1503 Size: 635 868 BglI GCCn_nnn'nGGC Cuts at: 0 113 795 1136 1252 1503 Size: 113 682 341 116 251 Fragments arranged by size: 682 341 251 116 113 BlpI GC'TnA_GC Cuts at: 0 691 859 1503 Size: 691 168 644 Fragments arranged by size: 691 644 168 Bme1580I G_kGCm'C Cuts at: 0 50 401 543 1503 Size: 50 351 142 960 Fragments arranged by size: 960 351 142 50 BmgBI CAC'GTC Cuts at: 0 516 1097 1503 Size: 516 581 406 Fragments arranged by size: 581 516 406 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1089 1503 Size: 1089 414 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 982 1503 Size: 982 521 Bpu10I CC'TnA_GC Cuts at: 0 55 953 1105 1503 Size: 55 898 152 398 Fragments arranged by size: 898 398 152 55 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 532 1503 Size: 532 971 BsaHI Gr'CG_yC Cuts at: 0 116 196 489 568 578 605 652 787 Size: 116 80 293 79 10 27 47 135 Cuts at: 787 1195 1231 1503 Size: 408 36 272 Fragments arranged by size: 408 293 272 135 116 80 79 47 36 27 10 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 436 466 1503 Size: 436 30 1037 Fragments arranged by size: 1037 436 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 547 1503 Size: 547 956 BseYI C'CCAG_C Cuts at: 0 1217 1223 1325 1374 1503 Size: 1217 6 102 49 129 Fragments arranged by size: 1217 129 102 49 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1011 1476 1503 Size: 1011 465 27 Fragments arranged by size: 1011 465 27 BsiEI CG_ry'CG Cuts at: 0 1033 1503 Size: 1033 470 BsiHKAI G_wGCw'C Cuts at: 0 776 1503 Size: 776 727 BsmBI CGTCTCn'nnnn_ Cuts at: 0 679 1503 Size: 679 824 Bsp1286I G_dGCh'C Cuts at: 0 50 401 543 697 776 865 1503 Size: 50 351 142 154 79 89 638 Fragments arranged by size: 638 351 154 142 89 79 50 BspHI T'CATG_A Cuts at: 0 824 1503 Size: 824 679 BsrBI CCG'CTC Cuts at: 0 161 537 1029 1088 1503 Size: 161 376 492 59 415 Fragments arranged by size: 492 415 376 161 59 BsrDI GCAATG_nn' Cuts at: 0 1425 1503 Size: 1425 78 BsrFI r'CCGG_y Cuts at: 0 491 666 745 1032 1126 1209 1370 1503 Size: 491 175 79 287 94 83 161 133 Fragments arranged by size: 491 287 175 161 133 94 83 79 BsrGI T'GTAC_A Cuts at: 0 1013 1503 Size: 1013 490 BssHII G'CGCG_C Cuts at: 0 7 704 1139 1503 Size: 7 697 435 364 Fragments arranged by size: 697 435 364 7 BstXI CCAn_nnnn'nTGG Cuts at: 0 36 111 1414 1503 Size: 36 75 1303 89 Fragments arranged by size: 1303 89 75 36 BstYI r'GATC_y Cuts at: 0 1444 1503 Size: 1444 59 Bsu36I CC'TnA_GG Cuts at: 0 200 1503 Size: 200 1303 BtgI C'CryG_G Cuts at: 0 110 191 245 782 1151 1173 1250 1503 Size: 110 81 54 537 369 22 77 253 Fragments arranged by size: 537 369 253 110 81 77 54 22 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 216 1018 1503 Size: 216 802 485 Fragments arranged by size: 802 485 216 EaeI y'GGCC_r Cuts at: 0 101 126 248 584 939 1030 1128 1176 Size: 101 25 122 336 355 91 98 48 Cuts at: 1176 1308 1503 Size: 132 195 Fragments arranged by size: 355 336 195 132 122 101 98 91 48 25 EagI C'GGCC_G Cuts at: 0 1030 1503 Size: 1030 473 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 349 866 1503 Size: 349 517 637 Fragments arranged by size: 637 517 349 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 792 982 1503 Size: 792 190 521 Fragments arranged by size: 792 521 190 EcoNI CCTnn'n_nnAGG Cuts at: 0 1063 1503 Size: 1063 440 EcoO109I rG'GnC_Cy Cuts at: 0 204 957 1068 1290 1503 Size: 204 753 111 222 213 Fragments arranged by size: 753 222 213 204 111 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 297 1503 Size: 297 1206 HaeII r_GCGC'y Cuts at: 0 199 492 501 581 664 790 907 1112 Size: 199 293 9 80 83 126 117 205 Cuts at: 1112 1198 1503 Size: 86 305 Fragments arranged by size: 305 293 205 199 126 117 86 83 80 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 436 468 637 669 1503 Size: 436 32 169 32 834 Fragments arranged by size: 834 436 169 32 32 KasI G'GCGC_C Cuts at: 0 195 488 577 786 1194 1503 Size: 195 293 89 209 408 309 Fragments arranged by size: 408 309 293 209 195 89 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 47 1503 Size: 47 1456 MscI TGG'CCA Cuts at: 0 103 250 1503 Size: 103 147 1253 Fragments arranged by size: 1253 147 103 MslI CAynn'nnrTG Cuts at: 0 244 679 1503 Size: 244 435 824 Fragments arranged by size: 824 435 244 MspA1I CmG'CkG Cuts at: 0 193 784 900 1252 1286 1427 1434 1503 Size: 193 591 116 352 34 141 7 69 Fragments arranged by size: 591 352 193 141 116 69 34 7 NaeI GCC'GGC Cuts at: 0 493 747 1034 1128 1211 1503 Size: 493 254 287 94 83 292 Fragments arranged by size: 493 292 287 254 94 83 NarI GG'CG_CC Cuts at: 0 196 489 578 787 1195 1503 Size: 196 293 89 209 408 308 Fragments arranged by size: 408 308 293 209 196 89 NcoI C'CATG_G Cuts at: 0 110 245 1503 Size: 110 135 1258 Fragments arranged by size: 1258 135 110 NgoMIV G'CCGG_C Cuts at: 0 491 745 1032 1126 1209 1503 Size: 491 254 287 94 83 294 Fragments arranged by size: 491 294 287 254 94 83 NspI r_CATG'y Cuts at: 0 465 999 1044 1503 Size: 465 534 45 459 Fragments arranged by size: 534 465 459 45 PasI CC'CwG_GG Cuts at: 0 36 1503 Size: 36 1467 PciI A'CATG_T Cuts at: 0 461 1040 1503 Size: 461 579 463 Fragments arranged by size: 579 463 461 PflMI CCAn_nnn'nTGG Cuts at: 0 1374 1503 Size: 1374 129 PpuMI rG'GwC_Cy Cuts at: 0 204 1290 1503 Size: 204 1086 213 Fragments arranged by size: 1086 213 204 PspOMI G'GGCC_C Cuts at: 0 539 1503 Size: 539 964 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 277 309 1503 Size: 277 32 1194 Fragments arranged by size: 1194 277 32 PstI C_TGCA'G Cuts at: 0 713 1503 Size: 713 790 PvuII CAG'CTG Cuts at: 0 1427 1434 1503 Size: 1427 7 69 Fragments arranged by size: 1427 69 7 SacII CC_GC'GG Cuts at: 0 194 785 1253 1503 Size: 194 591 468 250 Fragments arranged by size: 591 468 250 194 SexAI A'CCwGG_T Cuts at: 0 206 1503 Size: 206 1297 SfcI C'TryA_G Cuts at: 0 222 709 1440 1503 Size: 222 487 731 63 Fragments arranged by size: 731 487 222 63 SfoI GGC'GCC Cuts at: 0 197 490 579 788 1196 1503 Size: 197 293 89 209 408 307 Fragments arranged by size: 408 307 293 209 197 89 SgrAI Cr'CCGG_yG Cuts at: 0 491 1503 Size: 491 1012 SmaI CCC'GGG Cuts at: 0 866 1242 1503 Size: 866 376 261 Fragments arranged by size: 866 376 261 SmlI C'TyrA_G Cuts at: 0 547 1503 Size: 547 956 SphI G_CATG'C Cuts at: 0 999 1503 Size: 999 504 StyI C'CwwG_G Cuts at: 0 110 245 521 572 611 677 1503 Size: 110 135 276 51 39 66 826 Fragments arranged by size: 826 276 135 110 66 51 39 TatI w'GTAC_w Cuts at: 0 140 1013 1503 Size: 140 873 490 Fragments arranged by size: 873 490 140 XmaI C'CCGG_G Cuts at: 0 864 1240 1503 Size: 864 376 263 Fragments arranged by size: 864 376 263 Enzymes that do cut: AcuI AfeI AflIII AleI AlfI AlwNI ApaI AvaI BanI BanII BbeI BclI BglI BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BsrBI BsrDI BsrFI BsrGI BssHII BstXI BstYI Bsu36I BtgI BtgZI EaeI EagI EciI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PpuMI PspOMI PsrI PstI PvuII SacII SexAI SfcI SfoI SgrAI SmaI SmlI SphI StyI TatI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AgeI AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BfrBI BglII BmrI BmtI BsaI BsaAI BsaBI BsaWI BsiWI BsmI BspEI BspMI BssSI BstAPI BstBI BstEII BstZ17I BtsI ClaI CspCI DraI DraIII DrdI EarI EcoICRI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NruI NsiI PacI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SfiI SgfI SnaBI SpeI SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmnI ZraI