Restriction map for
pFN21AE0434
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BanII BbeI BbrPI BbvCI BcgI
BciVI BcnI BfaI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmFI BsoBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstUI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII HapII HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI Ksp632I KspI
MaeI MaeII MfeI MluI MroI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI SwaI
TaqI Tth111I TthHB8I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AciI 100 GCGG CCGC(-3/-1)
AlwNI 221 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 940 GAGCAC G[AT]GC[AT]^C
BalI 872 TGGCCA TGG^CCA
BamHI 714 GGATCC G^GATCC
BanI 186 GGTGCC G^G[CT][GA]CC
BbsI 46 GTCTTC GAAGAC(2/6)
BclI 660 TGATCA T^GATCA
BmyI 940 GAGCAC G[AGT]GC[ACT]^C
BpmI 761 CTGGAG CTGGAG(16/14)
BpuAI 46 GTCTTC GAAGAC(2/6)
BsaBI 888 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 56 GAGACC GGTCTC(1/5)
BsiHKAI 940 GAGCAC G[AT]GC[AT]^C
BsiYI 503 CCAATGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 503 CCAATGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 940 GAGCAC G[AGT]GC[ACT]^C
BspHI 840 TCATGA T^CATGA
BstAPI 211 GCAGAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 314 GGTTACC G^GT[ACGT]ACC
BstPI 314 GGTTACC G^GT[ACGT]ACC
BstXI 582 CCATAGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 58 GACCAGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 872 TGGCCA [CT]^GGCC[GA]
EcoO65I 314 GGTTACC G^GT[ACGT]ACC
FbaI 660 TGATCA T^GATCA
HgaI 831 GACGC GACGC(5/10)
MamI 888 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 872 TGGCCA TGG^CCA
MscI 872 TGGCCA TGG^CCA
MseI 805 TTAA T^TAA
MslI 664 CATCGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 858 GCATGC [GA]CATG^[CT]
PflMI 503 CCAATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 209 CTGCAG CTGCA^G
RcaI 840 TCATGA T^CATGA
ScaI 643 AGTACT AGT^ACT
SmlI 380 CTCAAG C^T[CT][GA]AG
SphI 858 GCATGC GCATG^C
StuI 921 AGGCCT AGG^CCT
Tru9I 805 TTAA T^TAA
Van91I 503 CCAATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcsI 196 AAATTT [GA]^AATT[CT]
780 GAATTT
AfaI 13 GTAC GT^AC
644 GTAC
AocI 319 CCTGAGG CC^T[ACGT]AGG
439 CCTGAGG
ApoI 196 AAATTT [GA]^AATT[CT]
780 GAATTT
BglII 90 AGATCT A^GATCT
726 AGATCT
BsmI 498 GAATGC GAATGC(1/-1)
880 GCATTC
BssKI 137 CCTGG ^CC[ACGT]GG
696 CCAGG
BstF5I 447 GGATG GGATG(2/0)
887 GGATG
BstNI 137 CCTGG CC^[AT]GG
696 CCAGG
Bsu36I 319 CCTGAGG CC^T[ACGT]AGG
439 CCTGAGG
Eco81I 319 CCTGAGG CC^T[ACGT]AGG
439 CCTGAGG
EcoRII 137 CCTGG ^CC[AT]GG
696 CCAGG
EcoT14I 78 CCTTGG C^C[AT][AT]GG
543 CCAAGG
FokI 447 GGATG GGATG(9/13)
887 GGATG
MspA1I 33 CAGCTG C[AC]G^C[GT]G
61 CAGCTG
MvaI 137 CCTGG CC^[AT]GG
696 CCAGG
MwoI 211 GCAGAGATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
493 GCCCTGAATGC
PvuII 33 CAGCTG CAG^CTG
61 CAGCTG
RsaI 13 GTAC GT^AC
644 GTAC
ScrFI 137 CCTGG CC^[ACGT]GG
696 CCAGG
SfcI 209 CTGCAG C^T[GA][CT]AG
467 CTACAG
StyI 78 CCTTGG C^C[AT][AT]GG
543 CCAAGG
TspRI 299 CACTG CA[GC]TG[ACGT][ACGT]^
759 CACTG
XcmI 69 CCAACATACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
719 CCATGTTAGATCTGG
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AluI AlwI AvaII BbvI BsaJI
BseRI BsmAI BsrI BstYI Cac8I
Cfr13I DdeI DpnII Fnu4HI HaeIII
HinfI HphI ItaI MaeIII MboI
MboII MflI MnlI NdeII NlaIII
NlaIV Sau3AI Sau96I SfaNI TfiI
TseI Tsp45I Tsp509I XhoII