(Circular) (Six-base) MAPSORT of: pFN21AE0434.seq Check: 5560 from: 1 to: 5650 pFN21AE0434 5650 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3460 278 Size: 53 83 186 936 1924 2468 Fragments arranged by size: 2468 1924 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2993 1937 Size: 1056 4594 Acc65I G'GTAC_C Cuts at: 2971 2971 Size: 5650 AclI AA'CG_TT Cuts at: 1563 3777 4150 1563 Size: 2214 373 3063 Fragments arranged by size: 3063 2214 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2379 3713 5097 5365 1632 Size: 747 1334 1384 268 1917 Fragments arranged by size: 1917 1384 1334 747 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5453 AflIII A'CryG_T Cuts at: 1203 4549 5526 1203 Size: 3346 977 1327 Fragments arranged by size: 3346 1327 977 AgeI A'CCGG_T Cuts at: 4481 4481 Size: 5650 AhdI GACnn_n'nnGTC Cuts at: 4379 4379 Size: 5650 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5650 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5650 AlwNI CAG_nnn'CTG Cuts at: 2231 3393 4965 2231 Size: 1162 1572 2916 Fragments arranged by size: 2916 1572 1162 ApaLI G'TGCA_C Cuts at: 3707 4863 3707 Size: 1156 4494 ApoI r'AATT_y Cuts at: 1493 2201 2785 2959 3185 3337 5446 1493 Size: 708 584 174 226 152 2109 1697 Fragments arranged by size: 2109 1697 708 584 226 174 152 AseI AT'TA_AT Cuts at: 160 1032 4204 160 Size: 872 3172 1606 Fragments arranged by size: 3172 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5650 AvaI C'yCGr_G Cuts at: 1870 1943 2975 1870 Size: 73 1032 4545 Fragments arranged by size: 4545 1032 73 BamHI G'GATC_C Cuts at: 2719 2980 3347 2719 Size: 261 367 5022 Fragments arranged by size: 5022 367 261 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2191 2971 4426 618 Size: 325 217 627 404 780 1455 1842 Fragments arranged by size: 1842 1455 780 627 404 325 217 BanII G_rGCy'C Cuts at: 1391 2969 1391 Size: 1578 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2044 928 Size: 971 145 4534 Fragments arranged by size: 4534 971 145 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3841 3875 1903 Size: 34 1904 34 3678 Fragments arranged by size: 3678 1904 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3542 4752 3542 Size: 1210 4440 BclI T'GATC_A Cuts at: 1548 2665 1548 Size: 1117 4533 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4261 136 Size: 107 122 71 631 605 2589 1525 Fragments arranged by size: 2589 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2095 2731 5645 2095 Size: 636 2914 2100 Fragments arranged by size: 2914 2100 636 BlpI GC'TnA_GC Cuts at: 3067 3067 Size: 5650 Bme1580I G_kGCm'C Cuts at: 1790 3711 4867 1790 Size: 1921 1156 2573 Fragments arranged by size: 2573 1921 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5650 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3381 4339 442 Size: 416 53 83 399 24 1964 958 1753 Fragments arranged by size: 1964 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5650 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2787 4310 1239 Size: 1548 1523 2579 Fragments arranged by size: 2579 1548 1523 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2370 3775 4640 4938 2370 Size: 1405 865 298 3082 Fragments arranged by size: 3082 1405 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2055 4313 882 Size: 617 556 2258 2219 Fragments arranged by size: 2258 2219 617 556 BsaAI yAC'GTr Cuts at: 493 1204 3441 493 Size: 711 2237 2702 Fragments arranged by size: 2702 2237 711 BsaBI GATnn'nnATC Cuts at: 2897 5644 2897 Size: 2747 2903 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3457 3839 4474 275 Size: 53 83 186 936 1924 382 635 1451 Fragments arranged by size: 1924 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4083 4481 4755 4902 5313 4083 Size: 398 274 147 411 4420 Fragments arranged by size: 4420 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2763 2793 2934 2964 1178 Size: 30 165 30 1360 30 141 30 3864 Fragments arranged by size: 3864 1360 165 141 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2035 2281 2450 2462 2784 2787 1186 Size: 849 246 169 12 322 3 4049 Fragments arranged by size: 4049 849 322 246 169 12 3 BseYI C'CCAG_C Cuts at: 4853 5349 4853 Size: 496 5154 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5077 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3100 3861 4889 1540 Size: 275 186 1099 761 1028 2301 Fragments arranged by size: 2301 1099 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2949 3711 3796 4867 2949 Size: 762 85 1071 3732 Fragments arranged by size: 3732 1071 762 85 BsmI GAATG_Cn' Cuts at: 2509 2885 3161 3254 2509 Size: 376 276 93 4905 Fragments arranged by size: 4905 376 276 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2949 2969 3711 3796 4867 1391 Size: 399 1159 20 742 85 1071 2174 Fragments arranged by size: 2174 1159 1071 742 399 85 20 BspEI T'CCGG_A Cuts at: 5313 5313 Size: 5650 BspHI T'CATG_A Cuts at: 2845 3539 5269 2845 Size: 694 1730 3226 Fragments arranged by size: 3226 1730 694 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3005 844 Size: 2161 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3537 1394 Size: 429 1714 3507 Fragments arranged by size: 3507 1714 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4145 4319 65 Size: 1183 2897 174 1396 Fragments arranged by size: 2897 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4294 4481 1700 Size: 110 2484 187 2869 Fragments arranged by size: 2869 2484 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5650 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5650 BssSI C'ACGA_G Cuts at: 3710 4722 3710 Size: 1012 4638 BstAPI GCAn_nnn'nTGC Cuts at: 794 2222 794 Size: 1428 4222 BstBI TT'CG_AA Cuts at: 4454 4454 Size: 5650 BstEII G'GTnAC_C Cuts at: 1184 2319 1184 Size: 1135 4515 BstXI CCAn_nnnn'nTGG Cuts at: 2594 2594 Size: 5650 BstYI r'GATC_y Cuts at: 1972 2095 2605 2719 2731 2980 3347 3732 3749 Size: 123 510 114 12 249 367 385 17 Cuts at: 3749 5201 5287 5299 5645 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 510 385 367 346 249 123 114 86 17 12 12 Bsu36I CC'TnA_GG Cuts at: 2325 2445 2325 Size: 120 5530 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4159 Fragments arranged by size: 4159 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4911 Fragments arranged by size: 4911 683 56 BtsI GCAGTG_nn' Cuts at: 3166 3960 3980 3166 Size: 794 20 4836 Fragments arranged by size: 4836 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5650 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5615 DraI TTT'AAA Cuts at: 2954 2954 Size: 5650 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2069 4657 809 Size: 507 753 2588 1802 Fragments arranged by size: 2588 1802 753 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2877 3097 3986 Size: 54 1425 50 275 18 1047 220 889 Cuts at: 3986 5615 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1047 889 275 220 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3097 1537 Size: 275 1285 4090 Fragments arranged by size: 4090 1285 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3580 1353 Size: 2227 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4237 4611 4757 387 Size: 1064 138 2648 374 146 1280 Fragments arranged by size: 2648 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2379 2787 3713 4310 5097 5365 1239 Size: 393 747 408 926 597 787 268 1524 Fragments arranged by size: 1524 926 787 747 597 408 393 268 EcoRI G'AATT_C Cuts at: 2959 3337 2959 Size: 378 5272 FspI TGC'GCA Cuts at: 4156 4156 Size: 5650 HaeII r_GCGC'y Cuts at: 1678 4797 1678 Size: 3119 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2122 2154 2273 2305 2339 Size: 32 135 32 550 32 119 32 34 Cuts at: 2339 2371 2827 2859 4291 4323 4365 4397 1373 Size: 32 456 32 1432 32 42 32 2626 Fragments arranged by size: 2626 1432 550 456 135 119 42 34 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2994 3240 669 Size: 988 281 1056 246 3079 Fragments arranged by size: 3079 1056 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5650 HpaI GTT'AAC Cuts at: 3240 3240 Size: 5650 KpnI G_GTAC'C Cuts at: 2975 2975 Size: 5650 MfeI C'AATT_G Cuts at: 3249 3249 Size: 5650 MluI A'CGCG_T Cuts at: 5526 5526 Size: 5650 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2098 4764 4948 1305 Size: 793 2666 184 2007 Fragments arranged by size: 2666 2007 793 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2879 10 Size: 54 1425 343 1047 2781 Fragments arranged by size: 2781 1425 1047 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2673 3608 3967 4126 518 Size: 648 1507 935 359 159 2042 Fragments arranged by size: 2042 1507 935 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2040 2068 3066 3743 4891 5136 1606 Size: 434 28 998 677 1148 245 2120 Fragments arranged by size: 2120 1148 998 677 434 245 28 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4159 Fragments arranged by size: 4159 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5650 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5650 NotI GC'GGCC_GC Cuts at: 3097 3097 Size: 5650 NspI r_CATG'y Cuts at: 1132 1229 2867 3008 4553 1132 Size: 97 1638 141 1545 2229 Fragments arranged by size: 2229 1638 1545 141 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5650 PciI A'CATG_T Cuts at: 4549 4549 Size: 5650 PflMI CCAn_nnn'nTGG Cuts at: 1108 2514 1108 Size: 1406 4244 PmeI GTTT'AAAC Cuts at: 2954 2954 Size: 5650 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3719 3751 5232 5264 3719 Size: 32 1481 32 4105 Fragments arranged by size: 4105 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5650 PsiI TTA'TAA Cuts at: 3220 3220 Size: 5650 PstI C_TGCA'G Cuts at: 830 2218 3002 830 Size: 1388 784 3478 Fragments arranged by size: 3478 1388 784 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5650 PvuII CAG'CTG Cuts at: 2040 2068 2040 Size: 28 5622 SalI G'TCGA_C Cuts at: 1936 2992 1936 Size: 1056 4594 SbfI CC_TGCA'GG Cuts at: 3002 3002 Size: 5650 ScaI AGT'ACT Cuts at: 2650 3898 5610 2650 Size: 1248 1712 2690 Fragments arranged by size: 2690 1712 1248 SfcI C'TryA_G Cuts at: 826 1045 2214 2472 2998 4133 4814 5005 826 Size: 219 1169 258 526 1135 681 191 1471 Fragments arranged by size: 1471 1169 1135 681 526 258 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5650 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5650 SmaI CCC'GGG Cuts at: 1872 2977 1872 Size: 1105 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2385 3754 4655 4917 820 Size: 197 926 442 1369 901 262 1553 Fragments arranged by size: 1553 1369 926 901 442 262 197 SnaBI TAC'GTA Cuts at: 493 3441 493 Size: 2948 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5650 SphI G_CATG'C Cuts at: 1132 2867 3008 1132 Size: 1735 141 3774 Fragments arranged by size: 3774 1735 141 SspI AAT'ATT Cuts at: 5 52 3574 5 Size: 47 3522 2081 Fragments arranged by size: 3522 2081 47 StuI AGG'CCT Cuts at: 1517 2928 1517 Size: 1411 4239 StyI C'CwwG_G Cuts at: 513 1065 2004 2083 2548 3089 513 Size: 552 939 79 465 541 3074 Fragments arranged by size: 3074 939 552 541 465 79 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2631 3677 3694 3847 4032 4477 325 Size: 1255 1051 1046 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1051 1046 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2648 3896 5608 Size: 274 80 33 51 1443 671 1248 1712 Cuts at: 5608 96 Size: 138 Fragments arranged by size: 1712 1443 1248 671 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2763 2795 5610 5642 2763 Size: 32 2815 32 2771 Fragments arranged by size: 2815 2771 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5650 XbaI T'CTAG_A Cuts at: 2986 2986 Size: 5650 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2081 2731 2081 Size: 650 5000 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5650 XmaI C'CCGG_G Cuts at: 1870 2975 1870 Size: 1105 4545 XmnI GAAnn'nnTTC Cuts at: 3779 3779 Size: 5650 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3458 276 Size: 53 83 186 936 1924 2468 Fragments arranged by size: 2468 1924 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI