Restriction map for
pFN21AE0434
(Linear) (Six-base) MAPSORT of: KIEE0434.seq Check: 4689 from: 1 to: 945
KIEE0434.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 945
Size: 374 571
AlwNI CAG_nnn'CTG
Cuts at: 0 226 945
Size: 226 719
ApoI r'AATT_y
Cuts at: 0 196 780 945
Size: 196 584 165
Fragments arranged by size:
584 196 165
BamHI G'GATC_C
Cuts at: 0 714 945
Size: 714 231
BanI G'GyrC_C
Cuts at: 0 186 945
Size: 186 759
BbsI GAAGACnn'nnnn_
Cuts at: 0 39 945
Size: 39 906
BclI T'GATC_A
Cuts at: 0 660 945
Size: 660 285
BglII A'GATC_T
Cuts at: 0 90 726 945
Size: 90 636 219
Fragments arranged by size:
636 219 90
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 782 945
Size: 782 163
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 365 945
Size: 365 580
BsaI GGTCTCn'nnnn_
Cuts at: 0 50 945
Size: 50 895
BsaBI GATnn'nnATC
Cuts at: 0 892 945
Size: 892 53
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 758 788 945
Size: 758 30 157
Fragments arranged by size:
758 157 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 30 276 445 457 779 782 945
Size: 30 246 169 12 322 3 163
Fragments arranged by size:
322 246 169 163 30 12 3
BsiHKAI G_wGCw'C
Cuts at: 0 944 945
Size: 944 1
BsmI GAATG_Cn'
Cuts at: 0 504 880 945
Size: 504 376 65
Fragments arranged by size:
504 376 65
Bsp1286I G_dGCh'C
Cuts at: 0 944 945
Size: 944 1
BspHI T'CATG_A
Cuts at: 0 840 945
Size: 840 105
BstAPI GCAn_nnn'nTGC
Cuts at: 0 217 945
Size: 217 728
BstEII G'GTnAC_C
Cuts at: 0 314 945
Size: 314 631
BstXI CCAn_nnnn'nTGG
Cuts at: 0 589 945
Size: 589 356
BstYI r'GATC_y
Cuts at: 0 90 600 714 726 945
Size: 90 510 114 12 219
Fragments arranged by size:
510 219 114 90 12
Bsu36I CC'TnA_GG
Cuts at: 0 320 440 945
Size: 320 120 505
Fragments arranged by size:
505 320 120
DrdI GACnn_nn'nnGTC
Cuts at: 0 64 945
Size: 64 881
EaeI y'GGCC_r
Cuts at: 0 872 945
Size: 872 73
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 782 945
Size: 374 408 163
Fragments arranged by size:
408 374 163
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 117 149 268 300 334 366 822 854
Size: 117 32 119 32 34 32 456 32
Cuts at: 854 945
Size: 91
Fragments arranged by size:
456 119 117 91 34 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 945
Size: 93 852
MscI TGG'CCA
Cuts at: 0 874 945
Size: 874 71
MslI CAynn'nnrTG
Cuts at: 0 668 945
Size: 668 277
MspA1I CmG'CkG
Cuts at: 0 35 63 945
Size: 35 28 882
Fragments arranged by size:
882 35 28
NspI r_CATG'y
Cuts at: 0 862 945
Size: 862 83
PflMI CCAn_nnn'nTGG
Cuts at: 0 509 945
Size: 509 436
PstI C_TGCA'G
Cuts at: 0 213 945
Size: 213 732
PvuII CAG'CTG
Cuts at: 0 35 63 945
Size: 35 28 882
Fragments arranged by size:
882 35 28
ScaI AGT'ACT
Cuts at: 0 645 945
Size: 645 300
SfcI C'TryA_G
Cuts at: 0 209 467 945
Size: 209 258 478
Fragments arranged by size:
478 258 209
SmlI C'TyrA_G
Cuts at: 0 380 945
Size: 380 565
SphI G_CATG'C
Cuts at: 0 862 945
Size: 862 83
StuI AGG'CCT
Cuts at: 0 923 945
Size: 923 22
StyI C'CwwG_G
Cuts at: 0 78 543 945
Size: 78 465 402
Fragments arranged by size:
465 402 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 626 945
Size: 626 319
TatI w'GTAC_w
Cuts at: 0 643 945
Size: 643 302
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 758 790 945
Size: 758 32 155
Fragments arranged by size:
758 155 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 76 726 945
Size: 76 650 219
Fragments arranged by size:
650 219 76
Enzymes that do cut:
AcuI AlwNI ApoI BamHI BanI BbsI BclI BglII
BpmI BpuEI BsaI BsaBI BsaXI BseRI BsiHKAI BsmI
Bsp1286I BspHI BstAPI BstEII BstXI BstYI Bsu36I DrdI
EaeI Eco57MI Hin4I MmeI MscI MslI MspA1I NspI
PflMI PstI PvuII ScaI SfcI SmlI SphI StuI
StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BanII BbeI BbvCI
BcgI BciVI BfrBI BglI BlpI Bme1580I BmgBI BmrI
BmtI BplI Bpu10I BsaAI BsaHI BsaWI BseYI BsgI
BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII EagI EarI EciI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI Tth111I XbaI XhoI XmaI XmnI ZraI