Restriction map for
pFN21AF0417
(Circular) (Six-base) MAPSORT of: pFN21AF0417.seq Check: 2074 from: 1 to: 6322
pFN21AF0417 6322 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2073 3520 2073
Size: 1447 4875
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4132 278
Size: 53 83 186 936 2596 2468
Fragments arranged by size:
2596 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3665 1937
Size: 1728 4594
Acc65I G'GTAC_C
Cuts at: 3643 3643
Size: 6322
AclI AA'CG_TT
Cuts at: 1563 4449 4822 1563
Size: 2886 373 3063
Fragments arranged by size:
3063 2886 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2536 4385 5769 6037 1632
Size: 904 1849 1384 268 1917
Fragments arranged by size:
1917 1849 1384 904 268
AfeI AGC'GCT
Cuts at: 2324 2324
Size: 6322
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6125
AflIII A'CryG_T
Cuts at: 1203 2578 2580 5221 6198 1203
Size: 1375 2 2641 977 1327
Fragments arranged by size:
2641 1375 1327 977 2
AgeI A'CCGG_T
Cuts at: 5153 5153
Size: 6322
AhdI GACnn_n'nnGTC
Cuts at: 5051 5051
Size: 6322
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6322
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2579 1184
Size: 1395 4927
AlwNI CAG_nnn'CTG
Cuts at: 2662 4065 5637 2662
Size: 1403 1572 3347
Fragments arranged by size:
3347 1572 1403
ApaI G_GGCC'C
Cuts at: 2892 2928 3163 3184 3373 2892
Size: 36 235 21 189 5841
Fragments arranged by size:
5841 235 189 36 21
ApaLI G'TGCA_C
Cuts at: 2462 4379 5535 2462
Size: 1917 1156 3249
Fragments arranged by size:
3249 1917 1156
ApoI r'AATT_y
Cuts at: 1493 3631 3857 4009 6118 1493
Size: 2138 226 152 2109 1697
Fragments arranged by size:
2138 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4876 160
Size: 872 3844 1606
Fragments arranged by size:
3844 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6322
AvaI C'yCGr_G
Cuts at: 1870 1943 2885 2943 3017 3115 3156 3162 3214
Size: 73 942 58 74 98 41 6 52
Cuts at: 3214 3313 3647 1870
Size: 99 334 4545
Fragments arranged by size:
4545 942 334 99 98 74 73 58
52 41 6
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2567 2600 2567
Size: 33 6289
BamHI G'GATC_C
Cuts at: 2071 2760 3062 3652 4019 2071
Size: 689 302 590 367 4374
Fragments arranged by size:
4374 689 590 367 302
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2135 2878 2953 3350 3643
Size: 325 217 627 348 743 75 397 293
Cuts at: 3643 5098 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 743 627 397 348 325 293
217 75
BanII G_rGCy'C
Cuts at: 1391 2376 2892 2928 2981 3163 3184 3373 3641
Size: 985 516 36 53 182 21 189 268
Cuts at: 3641 1391
Size: 4072
Fragments arranged by size:
4072 985 516 268 189 182 53 36
21
BbeI G_GCGC'C
Cuts at: 2957 2957
Size: 6322
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2308 928
Size: 971 409 4942
Fragments arranged by size:
4942 971 409
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4513 4547 1903
Size: 34 2576 34 3678
Fragments arranged by size:
3678 2576 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3579 4214 5424 3579
Size: 635 1210 4477
Fragments arranged by size:
4477 1210 635
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6322
BfrBI ATG'CAT
Cuts at: 3045 3045
Size: 6322
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2673 2814
Size: 107 122 71 631 605 337 664 141
Cuts at: 2814 2887 4933 136
Size: 73 2046 1525
Fragments arranged by size:
2046 1525 664 631 605 337 141 122
107 73 71
BglII A'GATC_T
Cuts at: 2265 6317 2265
Size: 4052 2270
BlpI GC'TnA_GC
Cuts at: 3739 3739
Size: 6322
Bme1580I G_kGCm'C
Cuts at: 1790 2466 2883 2892 2928 3163 3184 3373 4383
Size: 676 417 9 36 235 21 189 1010
Cuts at: 4383 5539 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1010 676 417 235 189 36
21 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6322
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3185 3614 4053
Size: 416 53 83 399 24 1768 429 439
Cuts at: 4053 5011 442
Size: 958 1753
Fragments arranged by size:
1768 1753 958 439 429 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6322
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2236 2236
Size: 6322
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2254 3274 3280 3431 4982 1239
Size: 1015 1020 6 151 1551 2579
Fragments arranged by size:
2579 1551 1020 1015 151 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2116 4447 5312 5610 2116
Size: 2331 865 298 2828
Fragments arranged by size:
2828 2331 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2438 4985 882
Size: 617 939 2547 2219
Fragments arranged by size:
2547 2219 939 617
BsaAI yAC'GTr
Cuts at: 493 1204 2581 4113 493
Size: 711 1377 1532 2702
Fragments arranged by size:
2702 1532 1377 711
BsaBI GATnn'nnATC
Cuts at: 2270 6316 2270
Size: 4046 2276
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2954 4129 4511 5146
Size: 53 83 186 936 1421 1175 382 635
Cuts at: 5146 275
Size: 1451
Fragments arranged by size:
1451 1421 1175 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2074 2902 4755 5153 5427 5574 5985 2074
Size: 828 1853 398 274 147 411 2411
Fragments arranged by size:
2411 1853 828 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2069 2099 2837 2867 3247
Size: 30 165 30 666 30 738 30 380
Cuts at: 3247 3277 1178
Size: 30 4223
Fragments arranged by size:
4223 738 666 380 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2456 3428 1186
Size: 1270 972 4080
Fragments arranged by size:
4080 1270 972
BseYI C'CCAG_C
Cuts at: 2041 2723 2911 3026 3110 3497 5525 6021 2041
Size: 682 188 115 84 387 2028 496 2342
Fragments arranged by size:
2342 2028 682 496 387 188 115 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5749
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3772 4533 5561 1540
Size: 275 186 1771 761 1028 2301
Fragments arranged by size:
2301 1771 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2217 2466 3552 3558 4383 4468 5539 2217
Size: 249 1086 6 825 85 1071 3000
Fragments arranged by size:
3000 1086 1071 825 249 85 6
BsmI GAATG_Cn'
Cuts at: 3833 3926 3833
Size: 93 6229
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2217 2376 2466 2883 2892 2928 2981
Size: 399 427 159 90 417 9 36 53
Cuts at: 2981 3163 3184 3373 3552 3558 3641 4383 4468
Size: 182 21 189 179 6 83 742 85
Cuts at: 4468 5539 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 427 417 399 189 182
179 159 90 85 83 53 36 21
9 6
BspEI T'CCGG_A
Cuts at: 2074 2902 5985 2074
Size: 828 3083 2411
Fragments arranged by size:
3083 2411 828
BspHI T'CATG_A
Cuts at: 4211 5941 4211
Size: 1730 4592
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2073 2905 3520 3677 844
Size: 1229 832 615 157 3489
Fragments arranged by size:
3489 1229 832 615 157
BsrBI CCG'CTC
Cuts at: 1394 1823 2125 2220 3103 4209 1394
Size: 429 302 95 883 1106 3507
Fragments arranged by size:
3507 1106 883 429 302 95
BsrDI GCAATG_nn'
Cuts at: 65 1248 4817 4991 65
Size: 1183 3569 174 1396
Fragments arranged by size:
3569 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2024 3071 3446 4966 5153 1700
Size: 110 214 1047 375 1520 187 2869
Fragments arranged by size:
2869 1520 1047 375 214 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6322
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6322
BssSI C'ACGA_G
Cuts at: 3543 3551 3557 4382 5394 3543
Size: 8 6 825 1012 4471
Fragments arranged by size:
4471 1012 825 8 6
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6322
BstBI TT'CG_AA
Cuts at: 5126 5126
Size: 6322
BstEII G'GTnAC_C
Cuts at: 1184 3507 1184
Size: 2323 3999
BstXI CCAn_nnnn'nTGG
Cuts at: 2188 2863 2188
Size: 675 5647
BstYI r'GATC_y
Cuts at: 1972 2071 2265 2760 3062 3652 4019 4404 4421
Size: 99 194 495 302 590 367 385 17
Cuts at: 4421 5873 5959 5971 6317 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 590 495 385 367 346 302
194 99 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2055 3345 2055
Size: 1290 5032
BtgI C'CryG_G
Cuts at: 513 1065 2115 3572 3586 513
Size: 552 1050 1457 14 3249
Fragments arranged by size:
3249 1457 1050 552 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5583
Fragments arranged by size:
5583 683 56
BtsI GCAGTG_nn'
Cuts at: 3838 4632 4652 3838
Size: 794 20 5508
Fragments arranged by size:
5508 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6322
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6287
DraI TTT'AAA
Cuts at: 3626 3626
Size: 6322
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5329 809
Size: 507 4013 1802
Fragments arranged by size:
4013 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2533 2775 3033
Size: 54 1425 50 275 18 703 242 258
Cuts at: 3033 3104 3769 4658 6287 8
Size: 71 665 889 1629 43
Fragments arranged by size:
1629 1425 889 703 665 275 258 242
71 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3769 1537
Size: 275 1957 4090
Fragments arranged by size:
4090 1957 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2052 2595 2684 2969 4252 1353
Size: 699 543 89 285 1283 3423
Fragments arranged by size:
3423 1283 699 543 285 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2040 3433 4909 5283 5429 387
Size: 1064 138 451 1393 1476 374 146 1280
Fragments arranged by size:
1476 1393 1280 1064 451 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2254 2536 3274 3280 3431 4385 4982
Size: 393 622 282 738 6 151 954 597
Cuts at: 4982 5769 6037 1239
Size: 787 268 1524
Fragments arranged by size:
1524 954 787 738 622 597 393 282
268 151 6
EcoO109I rG'GnC_Cy
Cuts at: 2059 2185 2238 2924 3088 3119 3180 3181 3232
Size: 126 53 686 164 31 61 1 51
Cuts at: 3232 3242 3249 3330 3369 3370 2059
Size: 10 7 81 39 1 5011
Fragments arranged by size:
5011 686 164 126 81 61 53 51
39 31 10 7 1 1
EcoRI G'AATT_C
Cuts at: 3631 4009 3631
Size: 378 5944
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3475 3475
Size: 6322
FspI TGC'GCA
Cuts at: 4828 4828
Size: 6322
HaeII r_GCGC'y
Cuts at: 1678 2103 2326 2784 2957 5469 1678
Size: 425 223 458 173 2512 2531
Fragments arranged by size:
2531 2512 458 425 223 173
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2837 2869 3247 3279 4963
Size: 32 135 32 1265 32 378 32 1684
Cuts at: 4963 4995 5037 5069 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1684 1265 378 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3666 3912 669
Size: 988 281 1728 246 3079
Fragments arranged by size:
3079 1728 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6322
HpaI GTT'AAC
Cuts at: 3912 3912
Size: 6322
KasI G'GCGC_C
Cuts at: 2953 2953
Size: 6322
KpnI G_GTAC'C
Cuts at: 3647 3647
Size: 6322
MfeI C'AATT_G
Cuts at: 3921 3921
Size: 6322
MluI A'CGCG_T
Cuts at: 6198 6198
Size: 6322
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2289 5436 5620 1305
Size: 984 3147 184 2007
Fragments arranged by size:
3147 2007 984 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2535 10
Size: 54 1425 343 703 3797
Fragments arranged by size:
3797 1425 703 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2615 3044 4280 4639 4798 518
Size: 648 1449 429 1236 359 159 2042
Fragments arranged by size:
2042 1449 1236 648 429 359 159
MspA1I CmG'CkG
Cuts at: 1606 2232 2643 2758 2934 2960 3339 3738 4415
Size: 626 411 115 176 26 379 399 677
Cuts at: 4415 5563 5808 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 626 411 399 379 245
176 115 26
NaeI GCC'GGC
Cuts at: 2026 3073 2026
Size: 1047 5275
NarI GG'CG_CC
Cuts at: 2954 2954
Size: 6322
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5770
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6322
NgoMIV G'CCGG_C
Cuts at: 2024 3071 2024
Size: 1047 5275
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6322
NotI GC'GGCC_GC
Cuts at: 3769 3769
Size: 6322
NsiI A_TGCA'T
Cuts at: 3047 3047
Size: 6322
NspI r_CATG'y
Cuts at: 1132 1229 2470 2624 3045 3680 5225 1132
Size: 97 1241 154 421 635 1545 2229
Fragments arranged by size:
2229 1545 1241 635 421 154 97
PasI CC'CwG_GG
Cuts at: 1348 2063 1348
Size: 715 5607
PciI A'CATG_T
Cuts at: 5221 5221
Size: 6322
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6322
PfoI T'CCnGG_A
Cuts at: 2283 2820 3006 2283
Size: 537 186 5599
Fragments arranged by size:
5599 537 186
PmeI GTTT'AAAC
Cuts at: 3626 3626
Size: 6322
PmlI CAC'GTG
Cuts at: 2581 2581
Size: 6322
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4391 4423 5904 5936 4391
Size: 32 1481 32 4777
Fragments arranged by size:
4777 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2059 3242 3330 2059
Size: 1183 88 5051
Fragments arranged by size:
5051 1183 88
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6322
PsiI TTA'TAA
Cuts at: 3892 3892
Size: 6322
PspOMI G'GGCC_C
Cuts at: 2888 2924 3159 3180 3369 2888
Size: 36 235 21 189 5841
Fragments arranged by size:
5841 235 189 36 21
PstI C_TGCA'G
Cuts at: 830 2390 3096 3359 3378 3674 830
Size: 1560 706 263 19 296 3478
Fragments arranged by size:
3478 1560 706 296 263 19
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6322
PvuII CAG'CTG
Cuts at: 2960 2960
Size: 6322
SalI G'TCGA_C
Cuts at: 1936 3664 1936
Size: 1728 4594
SapI GCTCTTCn'nnn_
Cuts at: 2052 2969 2052
Size: 917 5405
SbfI CC_TGCA'GG
Cuts at: 3359 3378 3674 3359
Size: 19 296 6007
Fragments arranged by size:
6007 296 19
ScaI AGT'ACT
Cuts at: 4570 6282 4570
Size: 1712 4610
SfcI C'TryA_G
Cuts at: 826 1045 2386 2572 3092 3355 3374 3670 4805
Size: 219 1341 186 520 263 19 296 1135
Cuts at: 4805 5486 5677 826
Size: 681 191 1471
Fragments arranged by size:
1471 1341 1135 681 520 296 263 219
191 186 19
SfiI GGCCn_nnn'nGGCC
Cuts at: 2673 2673
Size: 6322
SfoI GGC'GCC
Cuts at: 2955 2955
Size: 6322
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6322
SgrAI Cr'CCGG_yG
Cuts at: 1700 3071 1700
Size: 1371 4951
SmaI CCC'GGG
Cuts at: 1872 2887 3019 3117 3158 3164 3216 3315 3649
Size: 1015 132 98 41 6 52 99 334
Cuts at: 3649 1872
Size: 4545
Fragments arranged by size:
4545 1015 334 132 99 98 52 41
6
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2095 4426 5327 5589 820
Size: 197 926 152 2331 901 262 1553
Fragments arranged by size:
2331 1553 926 901 262 197 152
SnaBI TAC'GTA
Cuts at: 493 4113 493
Size: 3620 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6322
SphI G_CATG'C
Cuts at: 1132 3680 1132
Size: 2548 3774
SrfI GCCC'GGGC
Cuts at: 3164 3315 3164
Size: 151 6171
SspI AAT'ATT
Cuts at: 5 52 4246 5
Size: 47 4194 2081
Fragments arranged by size:
4194 2081 47
StuI AGG'CCT
Cuts at: 1517 2606 2676 1517
Size: 1089 70 5163
Fragments arranged by size:
5163 1089 70
StyI C'CwwG_G
Cuts at: 513 1065 2181 2856 3237 3602 3761 513
Size: 552 1116 675 381 365 159 3074
Fragments arranged by size:
3074 1116 675 552 381 365 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2123 3124 4349 4366 4519 4704 5149
Size: 1255 543 1001 1225 17 153 185 445
Cuts at: 5149 325
Size: 1498
Fragments arranged by size:
1498 1255 1225 1001 543 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4568 6280 96
Size: 274 80 33 51 1443 2591 1712 138
Fragments arranged by size:
2591 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2069 2101 6282 6314 2069
Size: 32 4181 32 2077
Fragments arranged by size:
4181 2077 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3535 1316
Size: 2219 4103
XbaI T'CTAG_A
Cuts at: 3658 3658
Size: 6322
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2513 2513
Size: 6322
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6322
XmaI C'CCGG_G
Cuts at: 1870 2885 3017 3115 3156 3162 3214 3313 3647
Size: 1015 132 98 41 6 52 99 334
Cuts at: 3647 1870
Size: 4545
Fragments arranged by size:
4545 1015 334 132 99 98 52 41
6
XmnI GAAnn'nnTTC
Cuts at: 4451 4451
Size: 6322
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4130 276
Size: 53 83 186 936 2596 2468
Fragments arranged by size:
2596 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbvCI Bpu10I BsiWI BsmBI BstZ17I
DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI PacI
PspXI PsrI RsrII SacI SacII SanDI SexAI SwaI