(Circular) (Six-base) MAPSORT of: pFN21AF0414.seq Check: 2053 from: 1 to: 7879 pFN21AF0414 7879 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2338 3484 5077 2338 Size: 1146 1593 5140 Fragments arranged by size: 5140 1593 1146 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2742 5689 278 Size: 53 83 186 936 1206 2947 2468 Fragments arranged by size: 2947 2468 1206 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5222 1937 Size: 3285 4594 Acc65I G'GTAC_C Cuts at: 5200 5200 Size: 7879 AclI AA'CG_TT Cuts at: 1563 6006 6379 1563 Size: 4443 373 3063 Fragments arranged by size: 4443 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2087 3234 3335 3490 4093 5942 7326 7594 Size: 455 1147 101 155 603 1849 1384 268 Cuts at: 7594 1632 Size: 1917 Fragments arranged by size: 1917 1849 1384 1147 603 455 268 155 101 AfeI AGC'GCT Cuts at: 2339 2339 Size: 7879 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7682 AflIII A'CryG_T Cuts at: 1203 2616 4135 4137 6778 7755 1203 Size: 1413 1519 2 2641 977 1327 Fragments arranged by size: 2641 1519 1413 1327 977 2 AgeI A'CCGG_T Cuts at: 6710 6710 Size: 7879 AhdI GACnn_n'nnGTC Cuts at: 3226 6608 3226 Size: 3382 4497 AleI CACnn'nnGTG Cuts at: 1166 2814 1166 Size: 1648 6231 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4136 1184 Size: 2952 4927 AlwNI CAG_nnn'CTG Cuts at: 2065 2137 2539 2848 3532 4219 5622 7194 2065 Size: 72 402 309 684 687 1403 1572 2750 Fragments arranged by size: 2750 1572 1403 687 684 402 309 72 ApaI G_GGCC'C Cuts at: 2679 3421 4449 4485 4720 4741 4930 2679 Size: 742 1028 36 235 21 189 5628 Fragments arranged by size: 5628 1028 742 235 189 36 21 ApaLI G'TGCA_C Cuts at: 2516 2696 4019 5936 7092 2516 Size: 180 1323 1917 1156 3303 Fragments arranged by size: 3303 1917 1323 1156 180 ApoI r'AATT_y Cuts at: 1493 5188 5414 5566 7675 1493 Size: 3695 226 152 2109 1697 Fragments arranged by size: 3695 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6433 160 Size: 872 5401 1606 Fragments arranged by size: 5401 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7879 AvaI C'yCGr_G Cuts at: 1870 1943 2378 3361 3414 3803 4442 4500 4574 Size: 73 435 983 53 389 639 58 74 Cuts at: 4574 4672 4713 4719 4771 4870 5204 1870 Size: 98 41 6 52 99 334 4545 Fragments arranged by size: 4545 983 639 435 389 334 99 98 74 73 58 53 52 41 6 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4124 4157 4124 Size: 33 7846 BamHI G'GATC_C Cuts at: 4317 4619 5209 5576 4317 Size: 302 590 367 6620 Fragments arranged by size: 6620 590 367 302 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4435 4510 4907 5200 6655 Size: 325 217 627 2648 75 397 293 1455 Cuts at: 6655 618 Size: 1842 Fragments arranged by size: 2648 1842 1455 627 397 325 293 217 75 BanII G_rGCy'C Cuts at: 1391 2480 2679 2691 3421 3933 4449 4485 4538 Size: 1089 199 12 730 512 516 36 53 Cuts at: 4538 4720 4741 4930 5198 1391 Size: 182 21 189 268 4072 Fragments arranged by size: 4072 1089 730 516 512 268 199 189 182 53 36 21 12 BbeI G_GCGC'C Cuts at: 4514 4514 Size: 7879 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3404 928 Size: 971 1505 5403 Fragments arranged by size: 5403 1505 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2926 2960 6070 6104 1903 Size: 34 989 34 3110 34 3678 Fragments arranged by size: 3678 3110 989 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5136 5771 6981 5136 Size: 635 1210 6034 Fragments arranged by size: 6034 1210 635 BclI T'GATC_A Cuts at: 1548 1548 Size: 7879 BfrBI ATG'CAT Cuts at: 3062 4602 3062 Size: 1540 6339 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4230 4371 4444 Size: 107 122 71 631 605 2558 141 73 Cuts at: 4444 6490 136 Size: 2046 1525 Fragments arranged by size: 2558 2046 1525 631 605 141 122 107 73 71 BglII A'GATC_T Cuts at: 7874 7874 Size: 7879 BlpI GC'TnA_GC Cuts at: 2540 5296 2540 Size: 2756 5123 Bme1580I G_kGCm'C Cuts at: 1790 2520 2679 2700 3421 4023 4440 4449 4485 Size: 730 159 21 721 602 417 9 36 Cuts at: 4485 4720 4741 4930 5940 7096 1790 Size: 235 21 189 1010 1156 2573 Fragments arranged by size: 2573 1156 1010 730 721 602 417 235 189 159 36 21 21 9 BmgBI CAC'GTC Cuts at: 1318 3223 1318 Size: 1905 5974 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2596 2780 2836 Size: 416 53 83 399 24 1179 184 56 Cuts at: 2836 4742 5171 5610 6568 442 Size: 1906 429 439 958 1753 Fragments arranged by size: 1906 1753 1179 958 439 429 416 399 184 83 56 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7879 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3271 3598 4831 4837 4988 6539 1239 Size: 2032 327 1233 6 151 1551 2579 Fragments arranged by size: 2579 2032 1551 1233 327 151 6 Bpu10I CC'TnA_GC Cuts at: 2111 2502 2111 Size: 391 7488 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2915 3731 3797 6004 6869 7167 2915 Size: 816 66 2207 865 298 3627 Fragments arranged by size: 3627 2207 865 816 298 66 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3506 3995 6542 882 Size: 617 2007 489 2547 2219 Fragments arranged by size: 2547 2219 2007 617 489 BsaAI yAC'GTr Cuts at: 493 1204 3742 4138 5670 493 Size: 711 2538 396 1532 2702 Fragments arranged by size: 2702 2538 1532 711 396 BsaBI GATnn'nnATC Cuts at: 7873 7873 Size: 7879 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2739 3482 4511 5686 Size: 53 83 186 936 1206 743 1029 1175 Cuts at: 5686 6068 6703 275 Size: 382 635 1451 Fragments arranged by size: 1451 1206 1175 1029 936 743 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3831 4459 6312 6710 6984 7131 7542 3831 Size: 628 1853 398 274 147 411 4168 Fragments arranged by size: 4168 1853 628 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3146 3176 3467 3497 3581 Size: 30 165 30 1743 30 291 30 84 Cuts at: 3581 3611 4394 4424 4804 4834 1178 Size: 30 783 30 380 30 4223 Fragments arranged by size: 4223 1743 783 380 291 165 84 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3038 3350 3586 4013 4985 1186 Size: 1852 312 236 427 972 4080 Fragments arranged by size: 4080 1852 972 427 312 236 BseYI C'CCAG_C Cuts at: 2221 2443 2667 2965 3877 4280 4468 4583 4667 Size: 222 224 298 912 403 188 115 84 Cuts at: 4667 5054 7082 7578 2221 Size: 387 2028 496 2522 Fragments arranged by size: 2522 2028 912 496 403 387 298 224 222 188 115 84 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2123 1163 Size: 573 387 6919 Fragments arranged by size: 6919 573 387 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5329 6090 7118 1540 Size: 275 186 3328 761 1028 2301 Fragments arranged by size: 3328 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2508 2520 2700 3831 4023 5109 5115 5940 6025 Size: 12 180 1131 192 1086 6 825 85 Cuts at: 6025 7096 2508 Size: 1071 3291 Fragments arranged by size: 3291 1131 1086 1071 825 192 180 85 12 6 BsmI GAATG_Cn' Cuts at: 5390 5483 5390 Size: 93 7786 BsmBI CGTCTCn'nnnn_ Cuts at: 3172 3172 Size: 7879 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2480 2508 2520 2679 2691 2700 3421 Size: 399 690 28 12 159 12 9 721 Cuts at: 3421 3831 3933 4023 4440 4449 4485 4538 4720 Size: 410 102 90 417 9 36 53 182 Cuts at: 4720 4741 4930 5109 5115 5198 5940 6025 7096 Size: 21 189 179 6 83 742 85 1071 Cuts at: 7096 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 721 690 417 410 399 189 182 179 159 102 90 85 83 53 36 28 21 12 12 9 9 6 BspEI T'CCGG_A Cuts at: 3831 4459 7542 3831 Size: 628 3083 4168 Fragments arranged by size: 4168 3083 628 BspHI T'CATG_A Cuts at: 5768 7498 5768 Size: 1730 6149 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2166 2289 2338 3484 4204 4462 5077 5234 Size: 1322 123 49 1146 720 258 615 157 Cuts at: 5234 844 Size: 3489 Fragments arranged by size: 3489 1322 1146 720 615 258 157 123 49 BsrBI CCG'CTC Cuts at: 1394 1823 4660 5766 1394 Size: 429 2837 1106 3507 Fragments arranged by size: 3507 2837 1106 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3265 6374 6548 65 Size: 1183 2017 3109 174 1396 Fragments arranged by size: 3109 2017 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2009 3544 3647 4628 5003 6523 6710 Size: 110 199 1535 103 981 375 1520 187 Cuts at: 6710 1700 Size: 2869 Fragments arranged by size: 2869 1535 1520 981 375 199 187 110 103 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7879 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7879 BssSI C'ACGA_G Cuts at: 2579 2945 5100 5108 5114 5939 6951 2579 Size: 366 2155 8 6 825 1012 3507 Fragments arranged by size: 3507 2155 1012 825 366 8 6 BstAPI GCAn_nnn'nTGC Cuts at: 794 2137 3826 794 Size: 1343 1689 4847 Fragments arranged by size: 4847 1689 1343 BstBI TT'CG_AA Cuts at: 6683 6683 Size: 7879 BstEII G'GTnAC_C Cuts at: 1184 2585 5064 1184 Size: 1401 2479 3999 Fragments arranged by size: 3999 2479 1401 BstXI CCAn_nnnn'nTGG Cuts at: 3451 4420 3451 Size: 969 6910 BstYI r'GATC_y Cuts at: 1972 2724 2940 4317 4619 5209 5576 5961 5978 Size: 752 216 1377 302 590 367 385 17 Cuts at: 5978 7430 7516 7528 7874 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1377 752 590 385 367 346 302 216 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4902 4902 Size: 7879 BtgI C'CryG_G Cuts at: 513 1065 2004 2701 3450 3886 5129 5143 513 Size: 552 939 697 749 436 1243 14 3249 Fragments arranged by size: 3249 1243 939 749 697 552 436 14 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7140 Fragments arranged by size: 7140 683 56 BtsI GCAGTG_nn' Cuts at: 2809 2821 2899 3090 5395 6189 6209 2809 Size: 12 78 191 2305 794 20 4479 Fragments arranged by size: 4479 2305 794 191 78 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7879 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7844 DraI TTT'AAA Cuts at: 5183 5183 Size: 7879 DraIII CAC_nnn'GTG Cuts at: 2515 2515 Size: 7879 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6886 809 Size: 507 5570 1802 Fragments arranged by size: 5570 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3262 3324 Size: 54 1425 50 275 18 177 1255 62 Cuts at: 3324 3453 3844 4090 4332 4590 4661 5326 6215 Size: 129 391 246 242 258 71 665 889 Cuts at: 6215 7844 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1255 889 665 391 275 258 246 242 177 129 71 62 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5326 1537 Size: 275 3514 4090 Fragments arranged by size: 4090 3514 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2226 2735 3210 4152 4241 4526 5809 1353 Size: 873 509 475 942 89 285 1283 3423 Fragments arranged by size: 3423 1283 942 873 509 475 285 89 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3703 4990 6466 6840 6986 387 Size: 1064 138 2114 1287 1476 374 146 1280 Fragments arranged by size: 2114 1476 1287 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 3234 3271 3335 3490 3598 4093 Size: 393 455 1147 37 64 155 108 495 Cuts at: 4093 4831 4837 4988 5942 6539 7326 7594 1239 Size: 738 6 151 954 597 787 268 1524 Fragments arranged by size: 1524 1147 954 787 738 597 495 455 393 268 155 151 108 64 37 6 EcoNI CCTnn'n_nnAGG Cuts at: 2948 2948 Size: 7879 EcoO109I rG'GnC_Cy Cuts at: 2125 2272 2629 3148 3418 3793 4481 4645 4676 Size: 147 357 519 270 375 688 164 31 Cuts at: 4676 4737 4738 4789 4799 4806 4887 4926 4927 Size: 61 1 51 10 7 81 39 1 Cuts at: 4927 2125 Size: 5077 Fragments arranged by size: 5077 688 519 375 357 270 164 147 81 61 51 39 31 10 7 1 1 EcoRI G'AATT_C Cuts at: 5188 5566 5188 Size: 378 7501 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2746 5032 2746 Size: 2286 5593 FspI TGC'GCA Cuts at: 6385 6385 Size: 7879 HaeII r_GCGC'y Cuts at: 1678 2341 3723 4341 4514 7026 1678 Size: 663 1382 618 173 2512 2531 Fragments arranged by size: 2531 2512 1382 663 618 173 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2867 2899 3212 3244 3467 Size: 32 135 32 1295 32 313 32 223 Cuts at: 3467 3499 4394 4426 4804 4836 6520 6552 6594 Size: 32 895 32 378 32 1684 32 42 Cuts at: 6594 6626 1373 Size: 32 2626 Fragments arranged by size: 2626 1684 1295 895 378 313 223 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5223 5469 669 Size: 988 281 3285 246 3079 Fragments arranged by size: 3285 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 3280 748 Size: 2532 5347 HpaI GTT'AAC Cuts at: 5469 5469 Size: 7879 KasI G'GCGC_C Cuts at: 4510 4510 Size: 7879 KpnI G_GTAC'C Cuts at: 5204 5204 Size: 7879 MfeI C'AATT_G Cuts at: 5478 5478 Size: 7879 MluI A'CGCG_T Cuts at: 7755 7755 Size: 7879 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6993 7177 1305 Size: 5688 184 2007 Fragments arranged by size: 5688 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3455 3846 4092 10 Size: 54 1425 343 1623 391 246 3797 Fragments arranged by size: 3797 1623 1425 391 343 246 54 MslI CAynn'nnrTG Cuts at: 518 1166 2319 2511 2814 3470 4172 4601 5837 Size: 648 1153 192 303 656 702 429 1236 Cuts at: 5837 6196 6355 518 Size: 359 159 2042 Fragments arranged by size: 2042 1236 1153 702 656 648 429 359 303 192 159 MspA1I CmG'CkG Cuts at: 1606 2134 2539 2649 3489 3700 3823 4200 4315 Size: 528 405 110 840 211 123 377 115 Cuts at: 4315 4491 4517 4896 5295 5972 7120 7365 1606 Size: 176 26 379 399 677 1148 245 2120 Fragments arranged by size: 2120 1148 840 677 528 405 399 379 377 245 211 176 123 115 110 26 NaeI GCC'GGC Cuts at: 2011 3546 3649 4630 2011 Size: 1535 103 981 5260 Fragments arranged by size: 5260 1535 981 103 NarI GG'CG_CC Cuts at: 4511 4511 Size: 7879 NcoI C'CATG_G Cuts at: 513 1065 2004 2701 513 Size: 552 939 697 5691 Fragments arranged by size: 5691 939 697 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7879 NgoMIV G'CCGG_C Cuts at: 2009 3544 3647 4628 2009 Size: 1535 103 981 5260 Fragments arranged by size: 5260 1535 981 103 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7879 NotI GC'GGCC_GC Cuts at: 5326 5326 Size: 7879 NsiI A_TGCA'T Cuts at: 3064 4604 3064 Size: 1540 6339 NspI r_CATG'y Cuts at: 1132 1229 2620 3763 4027 4181 4602 5237 6782 Size: 97 1391 1143 264 154 421 635 1545 Cuts at: 6782 1132 Size: 2229 Fragments arranged by size: 2229 1545 1391 1143 635 421 264 154 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7879 PciI A'CATG_T Cuts at: 2616 6778 2616 Size: 4162 3717 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7879 PfoI T'CCnGG_A Cuts at: 4377 4563 4377 Size: 186 7693 PmeI GTTT'AAAC Cuts at: 5183 5183 Size: 7879 PmlI CAC'GTG Cuts at: 4138 4138 Size: 7879 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5948 5980 7461 7493 5948 Size: 32 1481 32 6334 Fragments arranged by size: 6334 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2125 2272 2629 3148 3793 4799 4887 2125 Size: 147 357 519 645 1006 88 5117 Fragments arranged by size: 5117 1006 645 519 357 147 88 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7879 PsiI TTA'TAA Cuts at: 5449 5449 Size: 7879 PspOMI G'GGCC_C Cuts at: 2675 3417 4445 4481 4716 4737 4926 2675 Size: 742 1028 36 235 21 189 5628 Fragments arranged by size: 5628 1028 742 235 189 36 21 PstI C_TGCA'G Cuts at: 830 2133 2337 2349 2637 2816 3537 3699 3947 Size: 1303 204 12 288 179 721 162 248 Cuts at: 3947 4653 4916 4935 5231 830 Size: 706 263 19 296 3478 Fragments arranged by size: 3478 1303 721 706 296 288 263 248 204 179 162 19 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7879 PvuII CAG'CTG Cuts at: 2134 3489 3700 3823 4517 2134 Size: 1355 211 123 694 5496 Fragments arranged by size: 5496 1355 694 211 123 SalI G'TCGA_C Cuts at: 1936 5221 1936 Size: 3285 4594 SapI GCTCTTCn'nnn_ Cuts at: 4526 4526 Size: 7879 SbfI CC_TGCA'GG Cuts at: 2349 2637 4916 4935 5231 2349 Size: 288 2279 19 296 4997 Fragments arranged by size: 4997 2279 296 288 19 ScaI AGT'ACT Cuts at: 6127 7839 6127 Size: 1712 6167 SexAI A'CCwGG_T Cuts at: 3429 3429 Size: 7879 SfcI C'TryA_G Cuts at: 826 1045 2129 2296 2333 2345 2633 2812 3533 Size: 219 1084 167 37 12 288 179 721 Cuts at: 3533 3695 3943 4129 4649 4912 4931 5227 6362 Size: 162 248 186 520 263 19 296 1135 Cuts at: 6362 7043 7234 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1084 721 681 520 296 288 263 248 219 191 186 179 167 162 37 19 12 SfiI GGCCn_nnn'nGGCC Cuts at: 4230 4230 Size: 7879 SfoI GGC'GCC Cuts at: 4512 4512 Size: 7879 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7879 SgrAI Cr'CCGG_yG Cuts at: 1700 4628 1700 Size: 2928 4951 SmaI CCC'GGG Cuts at: 1872 3363 3416 3805 4444 4576 4674 4715 4721 Size: 1491 53 389 639 132 98 41 6 Cuts at: 4721 4773 4872 5206 1872 Size: 52 99 334 4545 Fragments arranged by size: 4545 1491 639 389 334 132 99 98 53 52 41 6 SmlI C'TyrA_G Cuts at: 820 1017 1943 2378 2930 3746 3812 5983 6884 Size: 197 926 435 552 816 66 2171 901 Cuts at: 6884 7146 820 Size: 262 1553 Fragments arranged by size: 2171 1553 926 901 816 552 435 262 197 66 SnaBI TAC'GTA Cuts at: 493 3742 5670 493 Size: 3249 1928 2702 Fragments arranged by size: 3249 2702 1928 SpeI A'CTAG_T Cuts at: 152 152 Size: 7879 SphI G_CATG'C Cuts at: 1132 5237 1132 Size: 4105 3774 SrfI GCCC'GGGC Cuts at: 3805 4721 4872 3805 Size: 916 151 6812 Fragments arranged by size: 6812 916 151 SspI AAT'ATT Cuts at: 5 52 5803 5 Size: 47 5751 2081 Fragments arranged by size: 5751 2081 47 StuI AGG'CCT Cuts at: 1517 4163 4233 1517 Size: 2646 70 5163 Fragments arranged by size: 5163 2646 70 StyI C'CwwG_G Cuts at: 513 1065 2004 2212 2701 2860 3516 4413 4794 Size: 552 939 208 489 159 656 897 381 Cuts at: 4794 5159 5318 513 Size: 365 159 3074 Fragments arranged by size: 3074 939 897 656 552 489 381 365 208 159 159 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2573 3473 3744 4681 5906 5923 6076 Size: 1255 993 900 271 937 1225 17 153 Cuts at: 6076 6261 6706 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1225 993 937 900 445 271 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3438 6125 7837 Size: 274 80 33 51 1443 1461 2687 1712 Cuts at: 7837 96 Size: 138 Fragments arranged by size: 2687 1712 1461 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3826 3858 7839 7871 3826 Size: 32 3981 32 3834 Fragments arranged by size: 3981 3834 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5092 1316 Size: 3776 4103 XbaI T'CTAG_A Cuts at: 5215 5215 Size: 7879 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4070 4070 Size: 7879 XhoI C'TCGA_G Cuts at: 1943 2378 1943 Size: 435 7444 XmaI C'CCGG_G Cuts at: 1870 3361 3414 3803 4442 4574 4672 4713 4719 Size: 1491 53 389 639 132 98 41 6 Cuts at: 4719 4771 4870 5204 1870 Size: 52 99 334 4545 Fragments arranged by size: 4545 1491 639 389 334 132 99 98 53 52 41 6 XmnI GAAnn'nnTTC Cuts at: 6008 6008 Size: 7879 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2740 5687 276 Size: 53 83 186 936 1206 2947 2468 Fragments arranged by size: 2947 2468 1206 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbvCI BplI BsiWI BstZ17I EcoICRI EcoRV FseI FspAI NruI PacI PspXI PsrI RsrII SacI SacII SanDI SwaI