################################## # NO CUT ENZYMES ( 179 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbsI BciVI BclI BcnI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HapII HhaI HinP1I HindIII HinfI HpaI HpaII KasI Ksp632I KspI MaeI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MspI MunI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### Acc65I 275 GGTACC G^GTACC AccII 343 CGCG CG^CG AcyI 127 GACGCC G[GA]^CG[CT]C AlwNI 331 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 418 CCTGAGG CC^T[ACGT]AGG ApaI 51 GGGCCC GGGCC^C Asp718I 275 GGTACC G^GTACC AspHI 297 GAGCAC G[AT]GC[AT]^C AvaII 288 GGTCC G^G[AT]CC BanI 275 GGTACC G^G[CT][GA]CC BcgI 144 GCAGCTCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaHI 127 GACGCC G[GA]^CG[CT]C BsaI 312 GGTCTC GGTCTC(1/5) BsiHKAI 297 GAGCAC G[AT]GC[AT]^C BsmAI 313 GTCTC GTCTC(1/5) BspMI 285 GCAGGT ACCTGC(4/8) BstUI 343 CGCG CG^CG Bsu36I 418 CCTGAGG CC^T[ACGT]AGG Cac8I 210 GCTGGC GC[ACGT]^[ACGT]GC DsaI 79 CCACGG C^C[GA][CT]GG Eco81I 418 CCTGAGG CC^T[ACGT]AGG FauI 161 CCCGC CCCGC(4/6) Hin1I 127 GACGCC G[GA]^CG[CT]C HincII 304 GTCAAC GT[CT]^[GA]AC HindII 304 GTCAAC GT[CT]^[GA]AC KpnI 275 GGTACC GGTAC^C MaeIII 167 GTCAC ^GT[ACGT]AC MboII 349 GAAGA GAAGA(8/7) MslI 327 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 139 CAGCTG C[AC]G^C[GT]G MvnI 343 CGCG CG^CG PflMI 330 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 287 AGGTCCC [GA]G^G[AT]CC[CT] PvuII 139 CAGCTG CAG^CTG SmlI 223 CTCAAG C^T[CT][GA]AG Tsp45I 167 GTCAC ^GT[GC]AC TspRI 201 CAGTG CA[GC]TG[ACGT][ACGT]^ Van91I 330 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AciI 162 CCGC CCGC(-3/-1) 237 CCGC AfaI 36 GTAC GT^AC 276 GTAC AluI 140 AGCT AG^CT 146 AGCT BanII 51 GGGCCC G[GA]GC[CT]^C 181 GAGCCC BbvCI 270 GCTGAGG CCTCAGC(-5/-2) 424 GCTGAGG BglI 205 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 236 GCCGCAAGGGC DraII 227 AGGCCCT [GA]G^G[ACGT]CC[CT] 287 AGGTCCC EcoNI 279 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 472 CCTGGTTCAGG EcoO109I 227 AGGCCCT [GA]G^G[ACGT]CC[CT] 287 AGGTCCC HgaI 127 GACGC GACGC(5/10) 267 GACGC NlaIII 91 CATG CATG^ 388 CATG PstI 85 CTGCAG CTGCA^G 142 CTGCAG RsaI 36 GTAC GT^AC 276 GTAC SfcI 85 CTGCAG C^T[GA][CT]AG 142 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### BbvI BmyI BpmI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI Cfr13I DdeI EcoRII Fnu4HI FokI HaeIII HphI ItaI MnlI MvaI MwoI NlaIV Sau96I ScrFI SfaNI TseI