(Circular) (Six-base) MAPSORT of: pFN21AF0156.seq Check: 9025 from: 1 to: 10279 pFN21AF0156 10279 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2961 2961 Size: 10279 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6114 7155 8089 278 Size: 53 83 186 936 4578 1041 934 2468 Fragments arranged by size: 4578 2468 1041 936 934 186 83 53 AccI GT'mk_AC Cuts at: 1937 3201 4313 5340 7622 1937 Size: 1264 1112 1027 2282 4594 Fragments arranged by size: 4594 2282 1264 1112 1027 Acc65I G'GTAC_C Cuts at: 2301 7600 2301 Size: 5299 4980 AclI AA'CG_TT Cuts at: 1563 8406 8779 1563 Size: 6843 373 3063 Fragments arranged by size: 6843 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2386 2574 2659 3122 3269 3392 3627 5618 Size: 754 188 85 463 147 123 235 1991 Cuts at: 5618 5934 6748 6769 7015 8342 9726 9994 1632 Size: 316 814 21 246 1327 1384 268 1917 Fragments arranged by size: 1991 1917 1384 1327 814 754 463 316 268 246 235 188 147 123 85 21 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 10082 AflIII A'CryG_T Cuts at: 1203 9178 10155 1203 Size: 7975 977 1327 Fragments arranged by size: 7975 1327 977 AgeI A'CCGG_T Cuts at: 9110 9110 Size: 10279 AhdI GACnn_n'nnGTC Cuts at: 3802 9008 3802 Size: 5206 5073 AleI CACnn'nnGTG Cuts at: 1166 2295 2969 6557 6917 7097 7426 1166 Size: 1129 674 3588 360 180 329 4019 Fragments arranged by size: 4019 3588 1129 674 360 329 180 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2987 7444 1184 Size: 1803 4457 4019 Fragments arranged by size: 4457 4019 1803 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2000 2032 2000 Size: 32 10247 AlwNI CAG_nnn'CTG Cuts at: 2381 2836 3394 3404 3736 3905 3952 4435 4459 Size: 455 558 10 332 169 47 483 24 Cuts at: 4459 6607 6792 7219 8022 9594 2381 Size: 2148 185 427 803 1572 3066 Fragments arranged by size: 3066 2148 1572 803 558 483 455 427 332 185 169 47 24 10 ApaI G_GGCC'C Cuts at: 3261 7247 7286 3261 Size: 3986 39 6254 Fragments arranged by size: 6254 3986 39 ApaLI G'TGCA_C Cuts at: 3337 8336 9492 3337 Size: 4999 1156 4124 Fragments arranged by size: 4999 4124 1156 ApoI r'AATT_y Cuts at: 1493 2355 5879 6251 7588 7814 7966 10075 1493 Size: 862 3524 372 1337 226 152 2109 1697 Fragments arranged by size: 3524 2109 1697 1337 862 372 226 152 AseI AT'TA_AT Cuts at: 160 1032 8833 160 Size: 872 7801 1606 Fragments arranged by size: 7801 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10279 AvaI C'yCGr_G Cuts at: 1870 1943 4676 6955 7604 1870 Size: 73 2733 2279 649 4545 Fragments arranged by size: 4545 2733 2279 649 73 BamHI G'GATC_C Cuts at: 3050 4836 7609 7976 3050 Size: 1786 2773 367 5353 Fragments arranged by size: 5353 2773 1786 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2301 3303 3347 3413 6475 Size: 325 217 627 514 1002 44 66 3062 Cuts at: 6475 7160 7600 9055 618 Size: 685 440 1455 1842 Fragments arranged by size: 3062 1842 1455 1002 685 627 514 440 325 217 66 44 BanII G_rGCy'C Cuts at: 1391 2463 2493 2770 3261 3502 3550 3705 4299 Size: 1072 30 277 491 241 48 155 594 Cuts at: 4299 4985 5857 6936 7247 7286 7469 7598 1391 Size: 686 872 1079 311 39 183 129 4072 Fragments arranged by size: 4072 1079 1072 872 686 594 491 311 277 241 183 155 129 48 39 30 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2425 4321 4353 4374 4415 5160 5530 Size: 971 526 1896 32 21 41 745 370 Cuts at: 5530 6710 7355 928 Size: 1180 645 3852 Fragments arranged by size: 3852 1896 1180 971 745 645 526 370 41 32 21 BbvCI CC'TCA_GC Cuts at: 2048 2663 3455 3671 3888 4070 4657 5073 6410 Size: 615 792 216 217 182 587 416 1337 Cuts at: 6410 6608 7437 2048 Size: 198 829 4890 Fragments arranged by size: 4890 1337 829 792 615 587 416 217 216 198 182 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6946 6980 8470 8504 1903 Size: 34 5009 34 1490 34 3678 Fragments arranged by size: 5009 3678 1490 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7079 8171 9381 7079 Size: 1092 1210 7977 Fragments arranged by size: 7977 1210 1092 BclI T'GATC_A Cuts at: 1548 5017 1548 Size: 3469 6810 BfrBI ATG'CAT Cuts at: 4243 4766 4243 Size: 523 9756 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2567 4681 6570 Size: 107 122 71 631 605 895 2114 1889 Cuts at: 6570 6960 8890 136 Size: 390 1930 1525 Fragments arranged by size: 2114 1930 1889 1525 895 631 605 390 122 107 71 BglII A'GATC_T Cuts at: 2628 2751 5502 10274 2628 Size: 123 2751 4772 2633 Fragments arranged by size: 4772 2751 2633 123 BlpI GC'TnA_GC Cuts at: 2492 6935 7696 2492 Size: 4443 761 5075 Fragments arranged by size: 5075 4443 761 Bme1580I G_kGCm'C Cuts at: 1790 3261 3341 3424 6480 6878 7247 7286 8340 Size: 1471 80 83 3056 398 369 39 1054 Cuts at: 8340 9496 1790 Size: 1156 2573 Fragments arranged by size: 3056 2573 1471 1156 1054 398 369 83 80 39 BmgBI CAC'GTC Cuts at: 1318 3184 4351 6043 6214 1318 Size: 1866 1167 1692 171 5383 Fragments arranged by size: 5383 1866 1692 1167 171 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2924 4195 5756 Size: 416 53 83 399 24 1507 1271 1561 Cuts at: 5756 7465 8010 8968 442 Size: 1709 545 958 1753 Fragments arranged by size: 1753 1709 1561 1507 1271 958 545 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10279 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4063 6106 4063 Size: 2043 8236 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2119 2507 2858 3308 3497 3617 3703 3750 Size: 880 388 351 450 189 120 86 47 Cuts at: 3750 3764 3776 4037 4481 4518 4939 5519 6076 Size: 14 12 261 444 37 421 580 557 Cuts at: 6076 8939 1239 Size: 2863 2579 Fragments arranged by size: 2863 2579 880 580 557 450 444 421 388 351 261 189 120 86 47 37 14 12 Bpu10I CC'TnA_GC Cuts at: 2048 2200 2663 2693 3123 3455 3671 3888 4070 Size: 152 463 30 430 332 216 217 182 Cuts at: 4070 4657 4979 4984 5073 6410 6608 7437 7463 Size: 587 322 5 89 1337 198 829 26 Cuts at: 7463 2048 Size: 4864 Fragments arranged by size: 4864 1337 829 587 463 430 332 322 217 216 198 182 152 89 30 26 5 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2708 3715 4538 7497 8404 9269 9567 2708 Size: 1007 823 2959 907 865 298 3420 Fragments arranged by size: 3420 2959 1007 907 865 823 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2082 3635 8942 882 Size: 617 583 1553 5307 2219 Fragments arranged by size: 5307 2219 1553 617 583 BsaAI yAC'GTr Cuts at: 493 1204 6078 7008 7099 8070 493 Size: 711 4874 930 91 971 2702 Fragments arranged by size: 4874 2702 971 930 711 91 BsaBI GATnn'nnATC Cuts at: 7310 10273 7310 Size: 2963 7316 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6111 7152 8086 8468 Size: 53 83 186 936 4578 1041 934 382 Cuts at: 8468 9103 275 Size: 635 1451 Fragments arranged by size: 4578 1451 1041 936 934 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5988 8712 9110 9384 9531 9942 5988 Size: 2724 398 274 147 411 6325 Fragments arranged by size: 6325 2724 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3950 3980 5308 5338 1178 Size: 30 165 30 2547 30 1328 30 6119 Fragments arranged by size: 6119 2547 1328 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3347 3680 3995 4556 5947 6735 1186 Size: 2161 333 315 561 1391 788 4730 Fragments arranged by size: 4730 2161 1391 788 561 333 315 BseYI C'CCAG_C Cuts at: 2395 2868 3329 3487 4125 4861 5109 5125 5591 Size: 473 461 158 638 736 248 16 466 Cuts at: 5591 5729 6505 6768 7285 9482 9978 2395 Size: 138 776 263 517 2197 496 2696 Fragments arranged by size: 2696 2197 776 736 638 517 496 473 466 461 263 248 158 138 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2228 3066 3918 4436 5089 5129 5267 Size: 573 492 838 852 518 653 40 138 Cuts at: 5267 5416 6236 6248 7045 1163 Size: 149 820 12 797 4397 Fragments arranged by size: 4397 852 838 820 797 653 573 518 492 149 138 40 12 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7729 8490 9518 1540 Size: 275 186 5728 761 1028 2301 Fragments arranged by size: 5728 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2463 2493 3341 3705 3753 4023 4185 4985 6077 Size: 30 848 364 48 270 162 800 1092 Cuts at: 6077 6936 7186 7469 8340 8425 9496 2463 Size: 859 250 283 871 85 1071 3246 Fragments arranged by size: 3246 1092 1071 871 859 848 800 364 283 270 250 162 85 48 30 BsmI GAATG_Cn' Cuts at: 2554 4148 7790 7883 2554 Size: 1594 3642 93 4950 Fragments arranged by size: 4950 3642 1594 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3217 3867 4538 6204 7159 3217 Size: 650 671 1666 955 6337 Fragments arranged by size: 6337 1666 955 671 650 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2463 2493 2770 3261 3341 3424 3502 Size: 399 673 30 277 491 80 83 78 Cuts at: 3502 3550 3705 3753 4023 4185 4299 4985 5857 Size: 48 155 48 270 162 114 686 872 Cuts at: 5857 6077 6480 6878 6936 7186 7247 7286 7469 Size: 220 403 398 58 250 61 39 183 Cuts at: 7469 7598 8340 8425 9496 1391 Size: 129 742 85 1071 2174 Fragments arranged by size: 2174 1071 872 742 686 673 491 403 399 398 277 270 250 220 183 162 155 129 114 85 83 80 78 61 58 48 48 39 30 BspEI T'CCGG_A Cuts at: 5988 9942 5988 Size: 3954 6325 BspHI T'CATG_A Cuts at: 8168 9898 8168 Size: 1730 8549 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2961 3389 5747 6150 7634 844 Size: 2117 428 2358 403 1484 3489 Fragments arranged by size: 3489 2358 2117 1484 428 403 BsrBI CCG'CTC Cuts at: 1394 1823 4534 7047 8166 1394 Size: 429 2711 2513 1119 3507 Fragments arranged by size: 3507 2711 2513 1119 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4243 5749 8774 8948 65 Size: 1183 2995 1506 3025 174 1396 Fragments arranged by size: 3025 2995 1506 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 8923 9110 1700 Size: 110 7113 187 2869 Fragments arranged by size: 7113 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2715 3966 96 Size: 2619 1251 6409 Fragments arranged by size: 6409 2619 1251 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10279 BssSI C'ACGA_G Cuts at: 5425 8339 9351 5425 Size: 2914 1012 6353 Fragments arranged by size: 6353 2914 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3736 3905 6362 794 Size: 2942 169 2457 4711 Fragments arranged by size: 4711 2942 2457 169 BstBI TT'CG_AA Cuts at: 9083 9083 Size: 10279 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 10279 BstXI CCAn_nnnn'nTGG Cuts at: 2155 2185 2804 2974 5971 6916 2155 Size: 30 619 170 2997 945 5518 Fragments arranged by size: 5518 2997 945 619 170 30 BstYI r'GATC_y Cuts at: 1972 2628 2751 3050 4836 5502 5833 7078 7609 Size: 656 123 299 1786 666 331 1245 531 Cuts at: 7609 7976 8361 8378 9830 9916 9928 10274 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1786 1452 1245 666 656 531 385 367 346 331 299 123 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2324 3594 2324 Size: 1270 9009 BtgI C'CryG_G Cuts at: 513 1065 4904 5328 5724 5847 7559 513 Size: 552 3839 424 396 123 1712 3233 Fragments arranged by size: 3839 3233 1712 552 424 396 123 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3878 5476 487 Size: 683 56 2652 1598 5290 Fragments arranged by size: 5290 2652 1598 683 56 BtsI GCAGTG_nn' Cuts at: 3116 3737 5353 6363 7795 8589 8609 3116 Size: 621 1616 1010 1432 794 20 4786 Fragments arranged by size: 4786 1616 1432 1010 794 621 20 ClaI AT'CG_AT Cuts at: 1554 4479 1554 Size: 2925 7354 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2623 2658 529 Size: 35 2059 35 8150 Fragments arranged by size: 8150 2059 35 35 DraI TTT'AAA Cuts at: 4092 5569 5896 7583 4092 Size: 1477 327 1687 6788 Fragments arranged by size: 6788 1687 1477 327 DraIII CAC_nnn'GTG Cuts at: 4638 5260 6067 4638 Size: 622 807 8850 Fragments arranged by size: 8850 807 622 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5927 7334 9286 809 Size: 507 4611 1407 1952 1802 Fragments arranged by size: 4611 1952 1802 1407 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2062 3435 3522 Size: 54 1425 50 275 18 232 1373 87 Cuts at: 3522 5284 5961 6571 6682 7221 7562 7726 8615 Size: 1762 677 610 111 539 341 164 889 Cuts at: 8615 10244 8 Size: 1629 43 Fragments arranged by size: 1762 1629 1425 1373 889 677 610 539 341 275 232 164 111 87 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7726 1537 Size: 275 5914 4090 Fragments arranged by size: 5914 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2892 3098 4013 4054 4515 4741 6065 7391 Size: 1539 206 915 41 461 226 1324 1326 Cuts at: 7391 8209 1353 Size: 818 3423 Fragments arranged by size: 3423 1539 1326 1324 915 818 461 226 206 41 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6045 8866 9240 9386 387 Size: 1064 138 4456 2821 374 146 1280 Fragments arranged by size: 4456 2821 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2461 2491 3703 4983 6934 7467 2461 Size: 30 1212 1280 1951 533 5273 Fragments arranged by size: 5273 1951 1280 1212 533 30 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2119 2386 2507 2574 2659 2858 3122 Size: 393 487 267 121 67 85 199 264 Cuts at: 3122 3269 3308 3392 3497 3617 3627 3703 3750 Size: 147 39 84 105 120 10 76 47 Cuts at: 3750 3764 3776 4037 4481 4518 4939 5519 5618 Size: 14 12 261 444 37 421 580 99 Cuts at: 5618 5934 6076 6748 6769 7015 8342 8939 9726 Size: 316 142 672 21 246 1327 597 787 Cuts at: 9726 9994 1239 Size: 268 1524 Fragments arranged by size: 1524 1327 787 672 597 580 487 444 421 393 316 268 267 264 261 246 199 147 142 121 120 105 99 85 84 76 67 47 39 37 21 14 12 10 EcoNI CCTnn'n_nnAGG Cuts at: 4831 5483 6974 7277 4831 Size: 652 1491 303 7833 Fragments arranged by size: 7833 1491 652 303 EcoO109I rG'GnC_Cy Cuts at: 2540 2918 3484 3811 4693 4975 5272 5776 6241 Size: 378 566 327 882 282 297 504 465 Cuts at: 6241 6274 6612 6759 6820 7243 7282 7459 7492 Size: 33 338 147 61 423 39 177 33 Cuts at: 7492 7521 2540 Size: 29 5298 Fragments arranged by size: 5298 882 566 504 465 423 378 338 327 297 282 177 147 61 39 33 33 29 EcoRI G'AATT_C Cuts at: 7588 7966 7588 Size: 378 9901 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2608 3287 3814 4121 4349 5219 2608 Size: 679 527 307 228 870 7668 Fragments arranged by size: 7668 870 679 527 307 228 FspI TGC'GCA Cuts at: 8785 8785 Size: 10279 HaeII r_GCGC'y Cuts at: 1678 9426 1678 Size: 7748 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3788 3820 4574 4606 4706 Size: 32 135 32 2216 32 754 32 100 Cuts at: 4706 4738 5408 5440 5777 5809 8920 8952 8994 Size: 32 670 32 337 32 3111 32 42 Cuts at: 8994 9026 1373 Size: 32 2626 Fragments arranged by size: 3111 2626 2216 754 670 337 135 100 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 7623 7869 669 Size: 988 281 5685 246 3079 Fragments arranged by size: 5685 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 5572 6034 748 Size: 4824 462 4993 Fragments arranged by size: 4993 4824 462 HpaI GTT'AAC Cuts at: 7869 7869 Size: 10279 KpnI G_GTAC'C Cuts at: 2305 7604 2305 Size: 5299 4980 MfeI C'AATT_G Cuts at: 7878 7878 Size: 10279 MluI A'CGCG_T Cuts at: 10155 10155 Size: 10279 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4239 4417 5388 5611 6837 9393 9577 1305 Size: 2934 178 971 223 1226 2556 184 2007 Fragments arranged by size: 2934 2556 2007 1226 971 223 184 178 MscI TGG'CCA Cuts at: 10 64 1489 1832 2064 3524 5286 6573 6684 Size: 54 1425 343 232 1460 1762 1287 111 Cuts at: 6684 7223 10 Size: 539 3066 Fragments arranged by size: 3066 1762 1460 1425 1287 539 343 232 111 54 MslI CAynn'nnrTG Cuts at: 518 1166 2036 2295 2955 2969 4028 4188 5969 Size: 648 870 259 660 14 1059 160 1781 Cuts at: 5969 6557 6917 7097 7426 8237 8596 8755 518 Size: 588 360 180 329 811 359 159 2042 Fragments arranged by size: 2042 1781 1059 870 811 660 648 588 360 359 329 259 180 160 159 14 MspA1I CmG'CkG Cuts at: 1606 2365 2662 3605 4435 5113 5726 6768 6789 Size: 759 297 943 830 678 613 1042 21 Cuts at: 6789 6858 7695 8372 9520 9765 1606 Size: 69 837 677 1148 245 2120 Fragments arranged by size: 2120 1148 1042 943 837 830 759 678 677 613 297 245 69 21 NcoI C'CATG_G Cuts at: 513 1065 4904 5847 7559 513 Size: 552 3839 943 1712 3233 Fragments arranged by size: 3839 3233 1712 943 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 10279 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10279 NotI GC'GGCC_GC Cuts at: 7726 7726 Size: 10279 NsiI A_TGCA'T Cuts at: 4245 4768 4245 Size: 523 9756 NspI r_CATG'y Cuts at: 1132 1229 2960 5455 5755 6190 7637 9182 1132 Size: 97 1731 2495 300 435 1447 1545 2229 Fragments arranged by size: 2495 2229 1731 1545 1447 435 300 97 PasI CC'CwG_GG Cuts at: 1348 3541 5772 6228 7489 1348 Size: 2193 2231 456 1261 4138 Fragments arranged by size: 4138 2231 2193 1261 456 PciI A'CATG_T Cuts at: 9178 9178 Size: 10279 PflMI CCAn_nnn'nTGG Cuts at: 1108 2311 2512 2836 3394 3520 3732 3952 4827 Size: 1203 201 324 558 126 212 220 875 Cuts at: 4827 7239 1108 Size: 2412 4148 Fragments arranged by size: 4148 2412 1203 875 558 324 220 212 201 126 PfoI T'CCnGG_A Cuts at: 3360 3753 4014 4839 5829 3360 Size: 393 261 825 990 7810 Fragments arranged by size: 7810 990 825 393 261 PmeI GTTT'AAAC Cuts at: 7583 7583 Size: 10279 PmlI CAC'GTG Cuts at: 6078 7008 7099 6078 Size: 930 91 9258 Fragments arranged by size: 9258 930 91 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8348 8380 9861 9893 8348 Size: 32 1481 32 8734 Fragments arranged by size: 8734 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2540 3484 3811 4693 5272 5776 6241 6759 6820 Size: 944 327 882 579 504 465 518 61 Cuts at: 6820 2540 Size: 5999 Fragments arranged by size: 5999 944 882 579 518 504 465 327 61 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 10279 PsiI TTA'TAA Cuts at: 7849 7849 Size: 10279 PspOMI G'GGCC_C Cuts at: 3257 7243 7282 3257 Size: 3986 39 6254 Fragments arranged by size: 6254 3986 39 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5750 5782 5750 Size: 32 10247 PstI C_TGCA'G Cuts at: 830 2479 2946 3231 3300 3450 4179 5487 5670 Size: 1649 467 285 69 150 729 1308 183 Cuts at: 5670 6027 6520 6603 7386 7546 7631 830 Size: 357 493 83 783 160 85 3478 Fragments arranged by size: 3478 1649 1308 783 729 493 467 357 285 183 160 150 85 83 69 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10279 PvuII CAG'CTG Cuts at: 2365 2662 3605 4435 5113 6768 6789 2365 Size: 297 943 830 678 1655 21 5855 Fragments arranged by size: 5855 1655 943 830 678 297 21 SacI G_AGCT'C Cuts at: 2463 2493 3705 4985 6936 7469 2463 Size: 30 1212 1280 1951 533 5273 Fragments arranged by size: 5273 1951 1280 1212 533 30 SacII CC_GC'GG Cuts at: 5727 5727 Size: 10279 SalI G'TCGA_C Cuts at: 1936 7621 1936 Size: 5685 4594 SanDI GG'GwC_CC Cuts at: 6241 6241 Size: 10279 SapI GCTCTTCn'nnn_ Cuts at: 3098 6065 3098 Size: 2967 7312 SbfI CC_TGCA'GG Cuts at: 2946 5487 7631 2946 Size: 2541 2144 5594 Fragments arranged by size: 5594 2541 2144 ScaI AGT'ACT Cuts at: 8527 10239 8527 Size: 1712 8567 SexAI A'CCwGG_T Cuts at: 5256 5982 5256 Size: 726 9553 SfcI C'TryA_G Cuts at: 826 1045 2475 2942 3227 3296 3446 4175 4738 Size: 219 1430 467 285 69 150 729 563 Cuts at: 4738 5483 5666 6023 6516 6592 6599 6717 7382 Size: 745 183 357 493 76 7 118 665 Cuts at: 7382 7474 7542 7627 8762 9443 9634 826 Size: 92 68 85 1135 681 191 1471 Fragments arranged by size: 1471 1430 1135 745 729 681 665 563 493 467 357 285 219 191 183 150 118 92 85 76 69 68 7 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10279 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10279 SmaI CCC'GGG Cuts at: 1872 4678 7606 1872 Size: 2806 2928 4545 Fragments arranged by size: 4545 2928 2806 SmlI C'TyrA_G Cuts at: 820 1017 1943 2723 3694 4553 7512 8383 9284 Size: 197 926 780 971 859 2959 871 901 Cuts at: 9284 9546 820 Size: 262 1553 Fragments arranged by size: 2959 1553 971 926 901 871 859 780 262 197 SnaBI TAC'GTA Cuts at: 493 8070 493 Size: 7577 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 10279 SphI G_CATG'C Cuts at: 1132 2960 6190 7637 1132 Size: 1828 3230 1447 3774 Fragments arranged by size: 3774 3230 1828 1447 SrfI GCCC'GGGC Cuts at: 4678 4678 Size: 10279 SspI AAT'ATT Cuts at: 5 52 8203 5 Size: 47 8151 2081 Fragments arranged by size: 8151 2081 47 StuI AGG'CCT Cuts at: 1517 2336 4559 5564 6011 6980 1517 Size: 819 2223 1005 447 969 4816 Fragments arranged by size: 4816 2223 1005 969 819 447 StyI C'CwwG_G Cuts at: 513 1065 2531 3519 3645 4689 4904 5847 5862 Size: 552 1466 988 126 1044 215 943 15 Cuts at: 5862 7559 7718 513 Size: 1697 159 3074 Fragments arranged by size: 3074 1697 1466 1044 988 943 552 215 159 126 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2239 2696 3046 3959 5123 5963 6020 Size: 1255 659 457 350 913 1164 840 57 Cuts at: 6020 6519 6591 7109 8306 8323 8476 8661 9106 Size: 499 72 518 1197 17 153 185 445 Cuts at: 9106 325 Size: 1498 Fragments arranged by size: 1498 1255 1197 1164 913 840 659 518 499 457 445 350 185 153 72 57 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2715 2994 3966 Size: 274 80 33 51 1443 738 279 972 Cuts at: 3966 8525 10237 96 Size: 4559 1712 138 Fragments arranged by size: 4559 1712 1443 972 738 279 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3042 3074 3414 3446 3476 3508 3574 3606 4685 Size: 32 340 32 30 32 66 32 1079 Cuts at: 4685 4717 5308 5340 5345 5377 10239 10271 3042 Size: 32 591 32 5 32 4862 32 3050 Fragments arranged by size: 4862 3050 1079 591 340 66 32 32 32 32 32 32 32 32 30 5 Tth111I GACn'n_nGTC Cuts at: 1316 4064 6290 6818 1316 Size: 2748 2226 528 4777 Fragments arranged by size: 4777 2748 2226 528 XbaI T'CTAG_A Cuts at: 7615 7615 Size: 10279 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3557 3845 5769 7439 3557 Size: 288 1924 1670 6397 Fragments arranged by size: 6397 1924 1670 288 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10279 XmaI C'CCGG_G Cuts at: 1870 4676 7604 1870 Size: 2806 2928 4545 Fragments arranged by size: 4545 2928 2806 XmnI GAAnn'nnTTC Cuts at: 2520 5825 8408 2520 Size: 3305 2583 4391 Fragments arranged by size: 4391 3305 2583 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6112 7153 8087 276 Size: 53 83 186 936 4578 1041 934 2468 Fragments arranged by size: 4578 2468 1041 936 934 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BsiWI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspXI RsrII SfiI SfoI SwaI