Restriction map for
pFN21AB6806
##################################
# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BbrPI BbsI
BcgI BciVI BclI BfrI BglII
BlnI BpmI BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BsiHKAI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BsrI BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI DsaI Eam1105I
EarI Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FokI FspI HgaI HincII HindII
HindIII HpaI KpnI Ksp632I KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MunI NcoI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tsp509I Tth111I
Van91I XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AcyI 16 GGCGCC G[GA]^CG[CT]C
AluI 184 AGCT AG^CT
AlwNI 509 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 173 GGGCCC GGGCC^C
AvaII 86 GGTCC G^G[AT]CC
BamHI 97 GGATCC G^GATCC
BanI 16 GGCGCC G^G[CT][GA]CC
BanII 173 GGGCCC G[GA]GC[CT]^C
BbeI 16 GGCGCC GGCGC^C
BbvCI 111 GCTGAGG CCTCAGC(-5/-2)
BglI 56 GCCCGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 156 GCTGAGC GC^T[ACGT]AGC
BmyI 173 GGGCCC G[AGT]GC[ACT]^C
Bpu1102I 156 GCTGAGC GC^T[ACGT]AGC
BsaHI 16 GGCGCC G[GA]^CG[CT]C
BseRI 397 GAGGAG GAGGAG(10/8)
BsgI 73 CTGCAC GTGCAG(16/14)
BsmFI 87 GTCCC GGGAC(10/14)
Bsp1286I 173 GGGCCC G[AGT]GC[ACT]^C
BssHII 30 GCGCGC G^CGCGC
BstNI 327 CCTGG CC^[AT]GG
BstXI 530 CCATTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 97 GGATCC [GA]^GATC[CT]
CelII 156 GCTGAGC GC^T[ACGT]AGC
DraII 85 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 85 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRII 327 CCTGG ^CC[AT]GG
FseI 60 GGCCGGCC GGCCGG^CC
HaeII 16 GGCGCC [GA]GCGC^[CT]
Hin1I 16 GGCGCC G[GA]^CG[CT]C
HinfI 259 GATTC G^A[ACGT]TC
KasI 16 GGCGCC G^GCGCC
MaeIII 344 GTCAC ^GT[ACGT]AC
MflI 97 GGATCC [GA]^GATC[CT]
MseI 235 TTAA T^TAA
MvaI 327 CCTGG CC^[AT]GG
NaeI 61 GCCGGC GCC^GGC
NarI 16 GGCGCC GG^CGCC
NdeI 422 CATATG CA^TATG
NgoMIV 61 GCCGGC G^CCGGC
PpuMI 85 GGGTCCC [GA]G^G[AT]CC[CT]
SfiI 55 GGCCCGGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 16 GGCGCC GGC^GCC
TfiI 259 GATTC G^A[AT]TC
Tru9I 235 TTAA T^TAA
Tsp45I 344 GTCAC ^GT[GC]AC
XbaI 291 TCTAGA T^CTAGA
XhoII 97 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 98 GATCC GGATC(4/5)
97 GGATC
BfaI 268 CTAG C^TAG
292 CTAG
BsiYI 62 CCGGCCGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
120 CCGGGGGCCGG
BslI 62 CCGGCCGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
120 CCGGGGGCCGG
EaeI 59 CGGCCG [CT]^GGCC[GA]
63 CGGCCG
EagI 59 CGGCCG C^GGCCG
63 CGGCCG
EclXI 59 CGGCCG C^GGCCG
63 CGGCCG
Eco52I 59 CGGCCG C^GGCCG
63 CGGCCG
MaeI 268 CTAG C^TAG
292 CTAG
MboII 190 GAAGA GAAGA(8/7)
358 GAAGA
MslI 364 CACATATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
531 CATTGATGTG
MspA1I 8 CCGCTG C[AC]G^C[GT]G
102 CAGCGG
NlaIII 314 CATG CATG^
408 CATG
NspI 313 GCATGT [GA]CATG^[CT]
407 ACATGC
SfaNI 382 GATGC GCATC(5/9)
517 GATGC
TaqI 413 TCGA T^CGA
495 TCGA
TspRI 390 CACTG CA[GC]TG[ACGT][ACGT]^
509 CAGTG
TthHB8I 413 TCGA T^CGA
495 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AccII AciI BbvI BcnI BsaJI
BsiEI BsrFI BssKI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
FauI Fnu4HI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MboI
MnlI MspI MvnI MwoI NciI
NdeII NlaIV Sau3AI Sau96I ScrFI
TseI