Restriction map for
pFN21AB6806
(Linear) (Six-base) MAPSORT of: KIBB6806.seq Check: 4414 from: 1 to: 552
KIBB6806.orf
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 514 552
Size: 514 38
ApaI G_GGCC'C
Cuts at: 0 177 552
Size: 177 375
BamHI G'GATC_C
Cuts at: 0 97 552
Size: 97 455
BanI G'GyrC_C
Cuts at: 0 16 552
Size: 16 536
BanII G_rGCy'C
Cuts at: 0 177 552
Size: 177 375
BbeI G_GCGC'C
Cuts at: 0 20 552
Size: 20 532
BbvCI CC'TCA_GC
Cuts at: 0 112 552
Size: 112 440
BglI GCCn_nnn'nGGC
Cuts at: 0 62 552
Size: 62 490
BlpI GC'TnA_GC
Cuts at: 0 157 552
Size: 157 395
Bme1580I G_kGCm'C
Cuts at: 0 177 552
Size: 177 375
Bpu10I CC'TnA_GC
Cuts at: 0 112 552
Size: 112 440
BsaHI Gr'CG_yC
Cuts at: 0 17 552
Size: 17 535
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 412 552
Size: 412 140
BseYI C'CCAG_C
Cuts at: 0 10 82 552
Size: 10 72 470
Fragments arranged by size:
470 72 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 552
Size: 58 494
BsiEI CG_ry'CG
Cuts at: 0 62 66 154 552
Size: 62 4 88 398
Fragments arranged by size:
398 88 62 4
Bsp1286I G_dGCh'C
Cuts at: 0 177 552
Size: 177 375
BsrFI r'CCGG_y
Cuts at: 0 61 65 149 552
Size: 61 4 84 403
Fragments arranged by size:
403 84 61 4
BssHII G'CGCG_C
Cuts at: 0 30 552
Size: 30 522
BstXI CCAn_nnnn'nTGG
Cuts at: 0 537 552
Size: 537 15
BstYI r'GATC_y
Cuts at: 0 97 552
Size: 97 455
EaeI y'GGCC_r
Cuts at: 0 59 63 552
Size: 59 4 489
Fragments arranged by size:
489 59 4
EagI C'GGCC_G
Cuts at: 0 59 63 552
Size: 59 4 489
Fragments arranged by size:
489 59 4
EcoO109I rG'GnC_Cy
Cuts at: 0 86 552
Size: 86 466
FseI GG_CCGG'CC
Cuts at: 0 65 552
Size: 65 487
HaeII r_GCGC'y
Cuts at: 0 20 552
Size: 20 532
KasI G'GCGC_C
Cuts at: 0 16 552
Size: 16 536
MslI CAynn'nnrTG
Cuts at: 0 368 535 552
Size: 368 167 17
Fragments arranged by size:
368 167 17
MspA1I CmG'CkG
Cuts at: 0 10 104 552
Size: 10 94 448
Fragments arranged by size:
448 94 10
NaeI GCC'GGC
Cuts at: 0 63 552
Size: 63 489
NarI GG'CG_CC
Cuts at: 0 17 552
Size: 17 535
NdeI CA'TA_TG
Cuts at: 0 423 552
Size: 423 129
NgoMIV G'CCGG_C
Cuts at: 0 61 552
Size: 61 491
NspI r_CATG'y
Cuts at: 0 317 411 552
Size: 317 94 141
Fragments arranged by size:
317 141 94
PpuMI rG'GwC_Cy
Cuts at: 0 86 552
Size: 86 466
PsiI TTA'TAA
Cuts at: 0 214 552
Size: 214 338
PspOMI G'GGCC_C
Cuts at: 0 173 552
Size: 173 379
SanDI GG'GwC_CC
Cuts at: 0 86 552
Size: 86 466
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 62 552
Size: 62 490
SfoI GGC'GCC
Cuts at: 0 18 552
Size: 18 534
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 70 102 552
Size: 70 32 450
Fragments arranged by size:
450 70 32
XbaI T'CTAG_A
Cuts at: 0 291 552
Size: 291 261
Enzymes that do cut:
AlwNI ApaI BamHI BanI BanII BbeI BbvCI BglI
BlpI Bme1580I Bpu10I BsaHI BseRI BseYI BsgI BsiEI
Bsp1286I BsrFI BssHII BstXI BstYI EaeI EagI EcoO109I
FseI HaeII KasI MslI MspA1I NaeI NarI NdeI
NgoMIV NspI PpuMI PsiI PspOMI SanDI SfiI SfoI
TstI XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BbsI BcgI
BciVI BclI BfrBI BglII BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrGI BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI NcoI NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TaqII TatI Tth111I XcmI XhoI XmaI
XmnI ZraI